CN108676746A - A kind of preparation method and product for integrating microorganism group microbial inoculum - Google Patents
A kind of preparation method and product for integrating microorganism group microbial inoculum Download PDFInfo
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- CN108676746A CN108676746A CN201810495113.1A CN201810495113A CN108676746A CN 108676746 A CN108676746 A CN 108676746A CN 201810495113 A CN201810495113 A CN 201810495113A CN 108676746 A CN108676746 A CN 108676746A
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- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 39
- 244000005700 microbiome Species 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000000855 fermentation Methods 0.000 claims abstract description 54
- 230000004151 fermentation Effects 0.000 claims abstract description 54
- 241000894006 Bacteria Species 0.000 claims abstract description 19
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 14
- 239000007787 solid Substances 0.000 claims abstract description 10
- 230000000813 microbial effect Effects 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 210000003608 fece Anatomy 0.000 claims abstract description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 7
- 239000010871 livestock manure Substances 0.000 claims abstract description 6
- 230000036284 oxygen consumption Effects 0.000 claims abstract description 6
- 238000012545 processing Methods 0.000 claims abstract description 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 5
- 238000004806 packaging method and process Methods 0.000 claims abstract description 5
- 239000000843 powder Substances 0.000 claims abstract description 5
- 238000003971 tillage Methods 0.000 claims abstract description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 16
- 241000282887 Suidae Species 0.000 claims description 8
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 8
- 230000010354 integration Effects 0.000 claims description 7
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 4
- 230000013872 defecation Effects 0.000 claims description 4
- 239000010903 husk Substances 0.000 claims description 4
- 235000014655 lactic acid Nutrition 0.000 claims description 4
- 239000004310 lactic acid Substances 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 244000144987 brood Species 0.000 claims description 2
- 244000060011 Cocos nucifera Species 0.000 claims 1
- 238000010564 aerobic fermentation Methods 0.000 abstract description 5
- 201000010099 disease Diseases 0.000 abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 5
- 235000015277 pork Nutrition 0.000 abstract 1
- 241000282898 Sus scrofa Species 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 4
- 238000009335 monocropping Methods 0.000 description 4
- 239000005416 organic matter Substances 0.000 description 4
- 241000737241 Cocos Species 0.000 description 3
- 241000227653 Lycopersicon Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000835 fiber Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
- 241001288350 Aerophilus Species 0.000 description 1
- 241001037822 Bacillus bacterium Species 0.000 description 1
- 241000268545 Bacillus eiseniae Species 0.000 description 1
- 241000363516 Bacillus filamentosus Species 0.000 description 1
- 241001357355 Bacillus kochii Species 0.000 description 1
- 241000025050 Bacillus rhizosphaerae Species 0.000 description 1
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 241001468261 Lysinibacillus macroides Species 0.000 description 1
- 241000361919 Metaphire sieboldi Species 0.000 description 1
- 241000634765 Oceanobacillus caeni Species 0.000 description 1
- 241001076676 Ornithinibacillus scapharcae Species 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 241001349191 Virgibacillus oceani Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 229910052804 chromium Inorganic materials 0.000 description 1
- 239000011651 chromium Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 208000012153 swine disease Diseases 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The present invention provides a kind of preparation method and product for integrating microorganism group microbial inoculum, and steps are as follows for the preparation method：The new bedding and padding of one layer of 10cm thickness are covered on the surface of fermentation bed, and the new bedding and padding include 25% 35% beancake powders and 65% 75% mushroom brans；50% 60%, daily pork chop just adds as nitrogen stream fermentation bed humid control, evens up bedding and padding with tractor rotary tillage mixing pig manure daily, carries out secondary solid oxygen consumption fermentation, operation 20 days of continuously fermenting；Fermentation ends enter product processing, take out the tunning of upper layer 20cm thickness, carry out dryness in the sun 5 days, when tunning water content<When 30%, into crushing, sub-sieve, packaging, is formed and integrate microorganism group microbial inoculum.Using microbial fermentation bed as fermentation tank, secondary aerobic fermentation produces high bacteria containing amount and integrates microorganism group microbial inoculum the present invention, for the biological control as plant disease, while realizing that dropping source application is discarded in cultivation.
Description
【Technical field】
The present invention relates to a kind of preparation methods and product for integrating microorganism group microbial inoculum.
【Background technology】
As modern agriculture industrial structure adjusts, specialization, compartmentalization and the large-scale planting area of crop are continuously increased,
But cultivated land resource is limited so that continuous cropping and multiple crop index are continuously increased, and the continuous cropping obstacles of getting worse have become limitation agricultural
Produce one of the bottleneck of sustainable development.The crop species that continuous cropping obstacle generation area is wide, is related to are more, gently then the underproduction caused to subtract
It receives, it is heavy then have no harvest, cause serious economic loss.Continuous cropping process farming operations and soil microbial community variation relation are close
It cuts, this case provides a kind of preparation method for integrating microorganism group microbial inoculum, secondary good using microbial fermentation bed as fermentation tank
Aerobe fermentation produces high bacteria containing amount and integrates microorganism group microbial inoculum, for the biological control as plant disease.Realize that cultivation is useless simultaneously
Abandon dropping source application.
【Invention content】
The technical problem to be solved in the present invention is to provide a kind of preparation method and product for integrating microorganism group microbial inoculum,
It is using microbial fermentation bed as fermentation tank, and secondary aerobic fermentation produces high bacteria containing amount and integrates microorganism group microbial inoculum, for making
For the biological control of plant disease, while realizing that dropping source application is discarded in cultivation.
The invention is realized in this way：
A kind of preparation method for integrating microorganism group microbial inoculum, steps are as follows for the preparation method：It is covered on the surface of fermentation bed
The new bedding and padding of one layer of 8-15cm thickness of lid, the new bedding and padding include 25%-35% beancake powders and 65%-75% mushroom brans；Fermentation bed is wet
In 50%-60%, daily growing and fattening pigs defecation adds as nitrogen stream, is evened up and is mixed with tractor rotary tillage mixing pig manure daily for degree control
New and old bedding and padding are closed, secondary solid oxygen consumption fermentation is carried out, operation 20-25 days of continuously fermenting；
Fermentation ends enter product processing, take out the tunning of upper layer 18-22cm thickness, carry out dryness in the sun 5-6 days, when
Tunning water content<When 30%, into crushing, sub-sieve, packaging, is formed and integrate microorganism group microbial inoculum；
Preparation method production solid fermentation tank used uses the big column pig raising microbial fermentation using 1 year or more
Bed, fermentation bed area are 1000-2000m2, fermentation groove depth 60-80cm；The fermentation tank is underground type, and fermenting bed padding is thick
Degree is 60-80cm；The basic formula of padding of the fermenting bed padding is that 25%-35% saws chaff, 15%-25% husks, 45%-
55% coconut palm chaff, contain in the fermenting bed padding bacillus generated in multiple-microorganism, including fermentation process, lactic acid bacteria and
Pseudomonad.
Further, the growing and fattening pigs are the 4-6 monthly ages, and cultivation density is 1.2-1.5m2/ pigs.
Further, the invention also includes a kind of above-mentioned preparation method for integrating microorganism group microbial inoculum is preparation-obtained whole
Close microorganism group microbial inoculum.
The invention has the advantages that：
The present invention makees strain inoculation using microbial fermentation bed as fermentation tank, using fermenting bed padding as microorganism group
Body and Source Organic Matter, addition integrate microorganism group fermentating formula and carry out the high bacteria containing amount integration microorganism of secondary aerobic fermentation production
Group microbial inoculum, can promote plant growth and disease prevention and control, for the biological control as plant disease, while realize that cultivation is discarded
The high-valued resource application of object.
The microbial inoculum quality that the present invention obtains：Microorganism group type is up to 1000 kinds or more, living bacteria count>3000000000/gram, wherein
Can cultivate bacillus bacterium number up to 200,000,000/gram, swine disease opportunistic pathogen is not detected；The content of organic matter 44.46%, total nitrogen content 2.23% are rotten
Grow acid content 11.2%, crude fiber content 14.06%.
【Specific implementation mode】
The present invention relates to a kind of preparation methods for integrating microorganism group microbial inoculum, and steps are as follows for the preparation method：It is fermenting
The surface of bed covers the new bedding and padding of one layer of 8-15cm cm thickness, and the new bedding and padding include 25%-35% beancake powders and 65%-75%
Mushroom bran；Fermentation bed humid control adds in 50%-60%, daily growing and fattening pigs defecation as nitrogen stream, and the growing and fattening pigs are the 4-6 months
Age, cultivation density 1.2-1.5m2/ pig.New and old bedding and padding are evened up and mixed with tractor rotary tillage mixing pig manure daily, are carried out secondary
Solid oxygen consumption ferments, operation 20-25 days of continuously fermenting；
Fermentation ends enter product processing, take out the tunning of upper layer 18-20cm thickness, carry out dryness in the sun 5-6 days, when
Tunning water content<When 30%, into crushing, sub-sieve, packaging, is formed and integrate microorganism group microbial inoculum；
Preparation method production solid fermentation tank used uses the big column pig raising microbial fermentation using 1 year or more
Bed, fermentation bed area are 1000-2000m2, fermentation groove depth 60-80cm；The fermentation tank is underground type, and fermenting bed padding is thick
Degree is 60-80cm；The basic formula of padding of the fermenting bed padding is that 25%-35% saws chaff, 15%-25% husks, 45%-
55% coconut palm chaff, contain in the fermenting bed padding bacillus generated in multiple-microorganism, including fermentation process, lactic acid bacteria and
Pseudomonad.
The invention further relates to a kind of above-mentioned products that preparation method that integrating microorganism group microbial inoculum is worth.
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment：
Using microbial fermentation bed as fermentation tank：Solid fermentation tank used uses micro- using big column pig raising in 1.5 years
Biological fermentation bed, fermentation bed area is 1500m2, fermentation groove depth 70cm；The fermentation tank is underground type, and fermenting bed padding is thick
Degree is 70cm；The basic formula of padding of the fermenting bed padding is 30% saw chaff, 20% husk, 50% coconut palm chaff, the fermentation bed
Contain the bacillus, lactic acid bacteria and pseudomonad generated in multiple-microorganism, including fermentation process, wherein brood cell's bar in bedding and padding
Strain class total content is up to 4.41 × 108cfu·g-1, provide Inoculant of the abundant microorganism group as microbial inoculum.
Secondary aerobic fermentation produces high bacteria containing amount and integrates microorganism group microbial inoculum：One layer of 10cm thickness is covered on the surface of fermentation bed
New bedding and padding, the new bedding and padding include 30% beancake powder and 70% mushroom bran；Fermentation bed humid control in 50%-60%,
Daily growing and fattening pigs defecation adds as nitrogen stream, and the growing and fattening pigs are 5 months monthly ages, cultivation density 1.5m2/ pig.
New and old bedding and padding are evened up and mixed with tractor rotary tillage mixing pig manure daily, carry out secondary solid oxygen consumption fermentation, operation of continuously fermenting
25 days；
Fermentation ends enter product processing, take out the tunning of upper layer 20cm thickness, carry out dryness in the sun 5 days, work as fermentation
Product water content<When 30%, into crushing, sub-sieve, packaging, is formed and integrate microorganism group microbial inoculum.
The above-mentioned integration microorganism group microbial inoculum product water content 29.74% produced, pH value 7.56, the content of organic matter
44.46%, total nitrogen content 2.23%, content of humic acid 11.2%, crude fiber content 14.06%, the bacillus of viable bacteria separation
Viable count 2.62 × 108cfu/g；Macro genome measurement result shows that the short sequence of microbial inoculum sample (reads) average value is
99701.75, every gram of microbial inoculum contains Bacteriophyta 39, and bacterium guiding principle 96,189, bacterium mesh, bacterium section 383, bacterium belongs to 786
It is a, bacterium kind 1281；46 kinds of the bacillus that macro genome detects, 9 kinds are new Chinese record species, i.e.,：①Bacillus
Aerophilus (thermophilic gas bacillus), 2. Bacillus eiseniae (earthworm bacillus), 3. Bacillus
Filamentosus (Filamentous bacillus), 4. Bacillus kochii (Ke He bacillus), 5. Bacillus
Rhizosphaerae (rhizosphere bacillus), 6. Lysinibacillus macroides (cow dung lysine bacillus), 7.
Oceanobacillus caeni (mud ocean bacillus), 8. Ornithinibacillus scapharcae (pick up clam bird
Propylhomoserin bacillus), 9. Virgibacillus oceani (ocean broadshoot bacillus)；Pig bacteria pathogeny is not found；Total abundance
It is 1.42%, the effective bacterium total content of microbial inoculum 280 × 10 in prediction on such basis8cfu/g。
Integrate Rectifying efficacies of the microorganism group microbial inoculum to bacterial wilt of tomato：It integrates microorganism group microbial inoculum leachate and handles mung bean
Germination percentage is 96.67%；Compared with clear water control treatment, radicle length 58.08% can be increased；It is educated with the microbial inoculum preparation of 5%-10%
Seedling matrix can significantly increase tomato emergence rate 3.5%, increase height growth 25.1%, to the Rectifying efficacies of bacterial wilt of tomato
Up to 79.41%.
Microorganism group microbial inoculum is integrated to determine：Organic matter >=40%, water content≤30%, pH5.5~7.5, excrement colibacillus group
≤ 100/g of number, induced worm egg death rate>95%, the term of validity>June；Content of beary metal meets standard requirement：Arsenic<15mg/kg, cadmium
≤ 15mg/kg, lead≤15mg/kg, chromium≤15mg/kg, mercury≤15mg/kg；Living bacteria count is adjusted to：Bacillus >=2 ×
108Cfu/g+ bacterial micro-organisms group >=30 × 108cfu/g.It integrates microorganism group microbial inoculum quality and meets Ministry of Agriculture's biological organic fertilizer agriculture
The requirement of industry professional standard (NY884-2012).
The present invention is formed using solid oxygen consumption fermentation processing and integrates microorganism group microbial inoculum, and method is simple, combines hair
Ferment bed to raise pig process effectively utilizes pig manure resource aerobic fermentation and produces high-valued integration microorganism group microbial inoculum, for as plant
The biological control of object disease.
Although specific embodiments of the present invention have been described above, those familiar with the art should manage
Solution, we are merely exemplary described specific embodiment, rather than for the restriction to the scope of the present invention, it is familiar with this
The technical staff in field modification and variation equivalent made by the spirit according to the present invention, should all cover the present invention's
In scope of the claimed protection.
Claims (3)
1. a kind of preparation method for integrating microorganism group microbial inoculum, it is characterised in that：Steps are as follows for the preparation method：In fermentation bed
Surface cover one layer of 8-15cm thickness new bedding and padding, the new bedding and padding include 25%-35% beancake powders and 65%-75% mushroom brans；
Fermentation bed humid control adds in 50%-60%, daily growing and fattening pigs defecation as nitrogen stream, daily with tractor rotary tillage mixing pig manure
New and old bedding and padding are evened up and mixed, secondary solid oxygen consumption fermentation is carried out, operation 20-25 days of continuously fermenting；
Fermentation ends enter product processing, take out the tunning of upper layer 18-22cm thickness, carry out dryness in the sun 5-6 days, work as fermentation
Product water content<When 30%, into crushing, sub-sieve, packaging, is formed and integrate microorganism group microbial inoculum；
Preparation method production solid fermentation tank used uses the big column pig raising microbial fermentation bed using 1 year or more, institute
It is underground type to state fermentation tank, and fermenting bed padding thickness is 60-80cm；The basic formula of padding of the fermenting bed padding is 25%-
35% saw chaff, 15%-25% husks, 45%-55% coconut palm chaffs contain multiple-microorganism, including brood cell in the fermenting bed padding
Bacillus, lactic acid bacteria and pseudomonad.
2. a kind of preparation method of integration microorganism group microbial inoculum according to claims 1, it is characterised in that：It is described to educate
Hog is 4-6 monthly ages, cultivation density 1.2-1.5m2/ pig.
3. a kind of integration microorganism group microbial inoculum, it is characterised in that：The integration microorganism group microbial inoculum is according to claims 1 or 2
A kind of preparation method of integration microorganism group microbial inoculum is preparation-obtained.
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| CN201810495113.1A CN108676746A (en) | 2018-05-22 | 2018-05-22 | A kind of preparation method and product for integrating microorganism group microbial inoculum |
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Family
ID=63807456
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Cited By (3)
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| CN110050709A (en) * | 2019-06-10 | 2019-07-26 | 刘坚 | A kind of fermenting bed padding that raising pregnant sow and its application and preparation method |
| CN110698229A (en) * | 2018-07-10 | 2020-01-17 | 领袖生物技术农业会社法人 | Preparation for producing fermentation liquid fertilizer with livestock and poultry manure odor reducing effect and fermentation liquid fertilizer circulating device for reducing livestock and poultry manure odor |
| CN110951650A (en) * | 2019-12-27 | 2020-04-03 | 原起科技(杭州)有限公司 | Bacillus khaki and application thereof |
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| CN110698229B (en) * | 2018-07-10 | 2022-03-08 | 领袖生物技术农业会社法人 | Preparation for producing fermentation liquid fertilizer with livestock and poultry manure odor reducing effect and fermentation liquid fertilizer circulating device for reducing livestock and poultry manure odor |
| CN110050709A (en) * | 2019-06-10 | 2019-07-26 | 刘坚 | A kind of fermenting bed padding that raising pregnant sow and its application and preparation method |
| CN110951650A (en) * | 2019-12-27 | 2020-04-03 | 原起科技(杭州)有限公司 | Bacillus khaki and application thereof |
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