CN107325991A - Composite bacteria agent of degraded kitchen garbage and preparation method thereof and the plastics and the clean method of foam class waste polluted by kitchen garbage - Google Patents
Composite bacteria agent of degraded kitchen garbage and preparation method thereof and the plastics and the clean method of foam class waste polluted by kitchen garbage Download PDFInfo
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- CN107325991A CN107325991A CN201710747788.6A CN201710747788A CN107325991A CN 107325991 A CN107325991 A CN 107325991A CN 201710747788 A CN201710747788 A CN 201710747788A CN 107325991 A CN107325991 A CN 107325991A
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- kitchen garbage
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- 241000894006 Bacteria Species 0.000 title claims abstract description 71
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- 239000006260 foam Substances 0.000 title claims abstract description 49
- 239000002699 waste material Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
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- 238000000034 method Methods 0.000 title claims abstract description 28
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 39
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- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
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- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical class [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The plastics and the clean method of foam class waste polluted the invention discloses a kind of composite bacteria agent for kitchen garbage of degrading and preparation method thereof and by kitchen garbage, the composite bacteria agent includes the strain of following parts by weight：28~33 parts of 18~29 parts of Candida lipolytica, 12~21 parts of aspergillus oryzae and bacillus licheniformis.The present invention using composite bacteria agent effectively degrade kitchen garbage realize kitchen garbage from polluted polybag, foam class waste surface efficiently peel off and fully, rapidly realize Biological clean purpose to having polluted polybag, foam class waste, the plastics after the processing of Biological clean method, foam class waste are recycled again, effectively reduce cost, the generation of harmful substance is greatly reduced, and is effectively simplified and has polluted polybag, foam class treatment of wastes produced flow.
Description
Technical field
Processing technology field is polluted the present invention relates to kitchen garbage, in particular to a kind of answering for kitchen garbage of degrading
Close microbial inoculum and preparation method thereof and the plastics and the clean method of foam class waste that are polluted by kitchen garbage.
Background technology
The polybag and the yield of foam class waste polluted every year by kitchen garbage is surprising, current traditional treatment method bag
Include：Plastics, foam class waste, which will have been polluted, will be sent directly into that incineration plant is burned, will to have polluted plastics, foam class waste straight
Pick into landfill yard carry out landfill disposal or with clear water will pollute plastics, the pollutant of foam class waste surface adhesion is washed off,
It is recycled.
But there is problems with above-mentioned three kinds of traditional processing methods：
(1) burn：The poisonous and harmful substance control effects such as a large amount of incineration flue gas , bioxin can be produced poor；Incineration plant is built
Operation cost is high.
(2) fill：Floor space is big；The organic matter of only surface adhesion is biodegradable, and plastics, foam class waste can not be certainly
So degraded；Percolate from garbage filling field amount is big, processing cost is high, easy polluted underground water.
(3) wash：A large amount of cleaning sullages, processing cost can be produced high；Plastics, foam class waste are difficult to clean up, clearly
Wash rear reuse value limited.
In a word, Cheap highly effective technology is lacked in the market to be effectively treated to having polluted plastics, foam class waste, and
There is cost height, take up an area big, heavy-polluted problem, do not possess marketing in traditional processing method as burned, filling or wash
Value.
The content of the invention
In view of this, composite bacteria agent of a kind of degraded kitchen garbage for providing of the present invention and preparation method thereof and by kitchen rubbish
The plastics of rubbish pollution and the clean method of foam class waste, preferably overcome the problem of above-mentioned prior art is present and defect,
Kitchen garbage of effectively being degraded using composite bacteria agent is realized kitchen garbage and efficiently shelled from polybag, foam class waste surface has been polluted
From and fully, rapidly realize Biological clean purpose to having polluted polybag, foam class waste, then Biological clean method will be passed through
Plastics, foam class waste after processing carry out recycling processing, effectively reduce cost, greatly reduce the production of harmful substance
It is raw, and be effectively simplified and polluted polybag, foam class treatment of wastes produced flow.
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 18~29
28~33 parts of part, 12~21 parts of aspergillus oryzae and bacillus licheniformis.
Further, the strain of following parts by weight is included：12~26 parts of Trichoderma viride.
Further, the strain of following parts by weight is included：10~22 parts of blown-ball Azotobacter.
Present invention also offers a kind of preparation method of the composite bacteria agent for kitchen garbage of degrading, the composite bacteria agent is above-mentioned
Composite bacteria agent；The preparation method includes：
Each strain is each added in kitchen garbage minimal medium, the culture medium is then positioned over culture shaking table
Middle progress domestication culture；
The composite bacteria agent that mixing compounding obtains degraded kitchen garbage will be carried out through each strain tamed after cultivating.
Further, the temperature of the culture shaking table is 25~35 DEG C.
Further, the rotating speed of the culture shaking table is 100~200rmin-1。
Further, the domestication incubation time is 3~8d.
Further, the preparation method includes：The domestication incubation is repeated 4~8 times.
Present invention also offers a kind of plastics polluted by kitchen garbage and the clean method of foam class waste, including：It is raw
Thing is cleaned；The Biological clean is inoculated on the plastics and foam class waste using above-mentioned composite bacteria agent and carries out biology clearly
It is clean.
Further, the mass ratio of the composite bacteria agent and the plastics and foam class waste is 1:800~1:1200.
Compared with prior art, composite bacteria agent of a kind of degraded kitchen garbage of the invention and preparation method thereof and by kitchen
The beneficial effect of the plastics of refuse pollution and the clean method of foam class waste is：
The present invention makes full use of the association of the strains such as Candida lipolytica, aspergillus oryzae and the bacillus licheniformis in composite bacteria agent
Same-action, can effectively degrade kitchen garbage, realize kitchen garbage and efficiently shelled from polybag, foam class waste surface has been polluted
From and fully, rapidly realize Biological clean purpose to having polluted polybag, foam class waste, then Biological clean method will be passed through
Plastics, foam class waste after processing are recycled, and are effectively reduced cost, are greatly reduced the generation of harmful substance, and
It is effectively simplified and has polluted polybag, foam class treatment of wastes produced flow, realizes the circular regeneration profit of polybag, foam class waste
With.
To enable the above objects, features and advantages of the present invention to become apparent, preferred embodiment cited below particularly makees detailed
It is described as follows.
Embodiment
For the ease of understanding the present invention, technical scheme is elaborated with reference to the mode of embodiment,
Many details are elaborated in the following description to fully understand the present invention.
But the invention can be embodied in many other ways as described herein, those skilled in the art can be with
Similar improvement is done in the case of without prejudice to intension of the present invention, therefore the present invention is not limited to the specific embodiments disclosed below.
Unless otherwise defined, all technologies used herein and scientific terminology have and the common skill of art of the present invention
The identical implication that art personnel are generally understood that.When there is contradiction, the definition in this specification is defined.
Term as used herein：
Term "comprising" used herein, " comprising ", " having ", " containing " or its any other deformation, it is intended that covering
Non-exclusionism includes.Wanted for example, composition, step, method, product or device comprising listed elements are not necessarily limited to those
Element, but can include not expressly listed other key elements or such a composition, step, method, product or device it is intrinsic
Key element.
Conjunction " by ... constitute " exclude any key element do not pointed out, step or component.If be used in claim,
This phrase will make claim be closed, it is not included the material in addition to materials of those descriptions, but relative
Except customary impurities.When phrase " by ... constitute " be rather than immediately following theme in the clause that appears in claim main body after
When, it is only limited to the key element described in the clause；Other key elements be not excluded as the overall claim it
Outside.
Equivalent, concentration or other values or parameter are excellent with scope, preferred scope or a series of upper limit preferred values and lower limit
During the Range Representation that choosing value is limited, this, which is appreciated that, specifically discloses by any range limit or preferred value and any scope
All scopes that any pairing of lower limit or preferred value is formed, regardless of whether whether the scope separately discloses.For example, when open
Scope " when 1~5 ", described scope should be interpreted as including scope " 1~4 ", " 1~3 ", " 1~2 ", " 1~2 and 4~
5 ", " 1~3 and 5 " etc..When number range is described herein, unless otherwise indicated, otherwise the scope is intended to include its end
Value and all integers and fraction within the range.
"and/or" is used to represent that one of illustrated situation or both may to occur, for example, A and/or B includes (A
And B) and (A or B).
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：
Such as 18 parts, 19 parts, 20 parts, 22 parts, 25 parts, 26 parts, 27 parts or 29 parts of 18~29 parts of Candida lipolytica；
Such as 12 parts, 13 parts, 14 parts, 15 parts, 16 parts, 17 parts, 18 parts, 19 parts, 20 parts or 21 parts of 12~21 parts of aspergillus oryzae；
With 28~33 parts such as 28 parts, 29 parts, 30 parts, 31 parts, 32 parts or 33 parts of bacillus licheniformis.
Preferably, the strain of following parts by weight is included：12~26 parts of Trichoderma viride as 12 parts, 15 parts, 18 parts, 20
Part, 22 parts, 24 parts or 26 parts etc..
Further, the strain of following parts by weight is included：10~22 parts of blown-ball Azotobacter as 10 parts, 12 parts, 15 parts,
18 parts, 20 parts or 22 parts etc..
It is to be appreciated that Candida lipolytica reduce fat it is very capable with protein.
Aspergillus oryzae be a class produce complex enzyme bacterial strain, except produce protease in addition to, can also produce amylase, carbohydrase, cellulase,
Phytase etc..In the presence of amylase, the straight chain in raw material, amylopectin are degraded to dextrin and various low molecule carbohydrates,
Such as maltose, glucose；In the presence of protease, macro-molecular protein can be degraded to peptone, polypeptide and various
Amino acid, and the mass degradation that can absorb the hardly possible such as crude fibre in auxiliary material, phytic acid.
Bacillus licheniformis cell form and arrangement are in shaft-like, Dan Sheng, and adjustable flora imbalance reaches therapeutic purposes, can promoted
Body is set to produce antibacterial substance, kill pathogenic bacteria；Resistant activity material can be produced, and takes oxygen effect machine by force with unique biology
System, can suppress the growth and breeding of pathogenic bacteria.In addition, bacillus licheniformis can decompose poisonous and harmful substance, purify water, with compared with
Strong protease, lipase, the activity of amylase, promote nutrient degraded.
Trichoderma viride is one kind of Trichoderma, belongs to Deuteromycotina, Hyphomycetes, hyphomycetales, spore mushroom is glued, in nature
It is widely distributed, it is often saprophytic on timber, seed and plant residue.Trichoderma viride can produce a variety of enzyme systems with bioactivity,
Such as：Cellulase, chitinase, zytase etc.；Trichoderma viride is one of active highest bacterial strain of cellulase-producing, produced
Cellulase have degradation to crop, effect is very good.
Blown-ball Azotobacter is Pseudomonadales, pseudomonadaceae, blown-ball Azotobacter category, is mainly used in bacterial fertilizer, has
Good nitrogenase activity, can effectively fixed nitrogen, and fixed nitrogen product ammonia directly can provide nitrogen source for homobium.
It is to be appreciated that the present invention is by directive breeding proliferative functionality microorganism fungus kind, can direct quick penetration
The incrustation scale and grease of pollutant (kitchen garbage), the interface that arrival polybag, foam class waste are contacted with kitchen garbage, at interface
Upper functional microbial strain is specifically degraded the target components such as starch, cellulose, fat, protein of kitchen garbage, together
When complete metabolism and the breeding of oneself.On the one hand, the vital movement of microorganism, which reduces to stick to, has polluted plastics
Bag, the kitchen garbage amount on foam class waste surface；On the other hand, microorganism fungus kind is decomposing biodegradable using kitchen garbage
During can produce a large amount of tiny bubbles, by physical shock and air supporting effect by a small amount of kitchen garbage from having polluted plastics
Bag, foam class waste are stripped out, and reach that kitchen garbage is separated with polybag, foam class waste has been polluted, while to having polluted
Polybag, foam class waste carry out the purpose of Biological clean recovery.
Present invention also offers a kind of preparation method of the composite bacteria agent for kitchen garbage of degrading, the composite bacteria agent is above-mentioned
Composite bacteria agent；The preparation method includes：
Each strain is each added in kitchen garbage minimal medium, the culture medium is then positioned over culture shaking table
Middle progress domestication culture；
The composite bacteria agent that mixing compounding obtains degraded kitchen garbage will be carried out through each strain tamed after cultivating.
Further, the temperature of the culture shaking table is 25~35 DEG C such as 25 DEG C, 28 DEG C, 30 DEG C, 32 DEG C or 35 DEG C.
Further, the rotating speed of the culture shaking table is 100~200rmin-1Such as 100rmin-1、120r·min-1、
150r·min-1、180r·min-1Or 200rmin-1Deng.
Further, the domestication incubation time is 3~8d such as 3d, 4d, 5d, 6d, 7d or 8d etc..
Further, the preparation method includes：By it is described domestication incubation repeat 4~8 times as 4 times, 5 times, 6 times, 7
It is secondary or 8 times.
It should be noted that above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis, Trichoderma viride and brown ball fixed nitrogen
Bacterium both can be purchased in market, oneself can also cultivate and obtain.
Preferably, the Candida lipolytica can by the following method be cultivated and obtained, and be specially：By Candida lipolytica
Freeze-dried powder is cultivated in YPD culture mediums, and the temperature of culture is 35~37 DEG C, and the time of culture is 5~8 days, then in 4~6 DEG C
Preserved in refrigerator, last enriched culture obtains Candida lipolytica pregnant solution.It should be noted that YPD culture mediums are called ferment
Female cream peptone glucose (YPD) agar medium, including 1% yeast extract, 2% peptone, 2% glucose and 2% agar powder.
Preferably, the aspergillus oryzae is isolated from composting production, is specially：Composting production is diluted to 100,000 times,
Then mixed with isolation medium, i.e. Czapek's medium (sodium nitrate 3g；Dipotassium hydrogen phosphate 1g；Epsom salt 0.5g；Potassium chloride
0.5g；Ferrous sulfate 0.01g；Sucrose 30g；Agar 20g；Distilled water 1000mL), dissolve by heating, 121 DEG C of sterilizings after packing
20min, then paves plate, and 28 DEG C incubated 7 days or so, and last enriched culture obtains aspergillus oryzae pregnant solution.
Preferably, the bacillus licheniformis is used as raw material from certain brewery's production delicate fragrance Daqu, brew house fermented grain
It is isolated, be specially：(1) LB culture mediums (tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, fine jade are first prepared
Fat 20g/L), the pH of the culture medium is adjusted with NaOH, 7.2~7.5 are reached, then the culture medium sterilized at 121 DEG C
Half an hour.(2) Daqu dry powder or fermented grain 1.5g are taken, is added in the sterilized water triangular flask with bead, shaking table culture half an hour,
Again 60 DEG C of water-bath half an hour, then dipped with oese, separation of being rule on LB culture medium flat plates, picking single bacterium colony continues to rule
Separation, untill obtaining the pure bacterial strain of single bacterium colony.(3) eventually pass enrichment culture and obtain bacillus licheniformis pregnant solution.
Preferably, the Trichoderma viride is obtained using following methods：Trichoderma viride bacterium powder is purchased, in PDA culture medium (horse
200 grams of bell potato, 20 grams of glucose, 15~20 grams of agar, running water 1L, natural pH) on draw flat board, cultivate the white bacterium that grows
Silk, Trichoderma viride spore, culture a period of time, long spore is more just into green.
Preferably, the blown-ball Azotobacter is obtained using following methods, is specially：(1) 6~8 centimetres are taken in appropriate garden
The soil of depth, is wrapped with aseptic paper, aseptically, is weighed 1g soil and is dissolved in 10ml sterilized waters, earthquake culture
5~10min is shaken in case, the soil liquid is obtained.(2) dip Soil Slurry with sterile spreader and be applied to culture medium (sweet dew
8~12g of alcohol, 0.1~0.4g of potassium dihydrogen phosphate, 0.2~0.4g of magnesium sulfate, 0.1~0.3g of sodium chloride, 0.2~0.5g of calcium sulfate,
Calcium carbonate 5-10g and distilled water 1L) among, it is placed in 28~30 DEG C of constant incubator and cultivates 3~4 days, treats occur on flat board
Mucoid colony, is purified in culture medium lining out until there is single bacterium colony.(3) last enriched culture obtains blown-ball Azotobacter
Nutrient solution.
It should be noted that kitchen garbage minimal medium be by kitchen garbage sample grind to form pasty state be added to it is inorganic
Obtained in salt culture medium；Wherein, the composition of minimal medium includes：0.0005g copper chlorides, 0.005g ferrous sulfate,
0.005g anhydrous Manganese chloride, 0.02g calcium chloride, 4g dipotassium hydrogen phosphates, 0.5g potassium dihydrogen phosphates, 1g anhydrous magnesium sulfates, 1g chlorinations
Sodium and 1000ml sterilized waters (pH is 7.5).
Above-mentioned enrichment culture, refers to since microorganism mixing group, the quantitative proportion of specific kind is constantly increased and guided into pure
A kind of cultural method of culture.The physiologic factor controls such as carbon source, nitrogen source, pH, temperature and the oxygen demand of the main basis of enrichment culture
System.
Present invention also offers a kind of plastics polluted by kitchen garbage and the clean method of foam class waste, including：It is raw
Thing is cleaned；The Biological clean is inoculated on the plastics and foam class waste using above-mentioned composite bacteria agent and carries out biology clearly
It is clean.
Further, the mass ratio of the composite bacteria agent and the plastics and foam class waste is 1:800~1:1200 such as
1:800、1:900、1:1000、1:1100 or 1:1200 etc..
Seen from the above description, the present invention makes full use of Candida lipolytica, aspergillus oryzae and the lichens bud in composite bacteria agent
The synergy of the strains such as spore bacillus, can effectively degrade kitchen garbage, realize kitchen garbage from having polluted polybag, foam
The Biological clean purpose to having polluted polybag, foam class waste is efficiently peeled off and fully, rapidly realized on class waste surface, then
Plastics after the processing of Biological clean method, foam class waste are recycled, cost is effectively reduced, has greatly reduced
The generation of evil material, and be effectively simplified and polluted polybag, foam class treatment of wastes produced flow, realize polybag, foam class
The recycling of waste.
For the ease of understanding the present invention, technical scheme is further illustrated with reference to embodiment.Applicant
Statement, the present invention illustrates detailed process equipment and the technological process of the present invention, but not office of the invention by following embodiments
It is limited to following detailed process equipments and technological process, that is, does not mean that the present invention should rely on following detailed process equipments and technique stream
Cheng Caineng is implemented.Person of ordinary skill in the field is it will be clearly understood that any improvement in the present invention, to each original of product of the present invention
The equivalence replacement of material and the addition of auxiliary element, the selection of concrete mode etc., all fall within protection scope of the present invention and open model
Within enclosing.
Embodiment 1
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 18 part, meter
33 parts of 12 parts of aspergillus and bacillus licheniformis.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) 18 parts of Candida lipolyticas, 12 parts of aspergillus oryzaes and 33 parts of bacillus licheniformis are each individually added into kitchen rubbish
In rubbish minimal medium, the culture medium is then positioned over 25 DEG C, domestication training is carried out in 100rmin-1 culture shaking table
It is a cycle to support 3d, so repeats to tame after 4 cycles, Candida lipolytica, aspergillus oryzae and lichens gemma bar are obtained respectively
The domestication product of bacterium.
(2) above-mentioned Candida lipolytica, aspergillus oryzae and the domestication of bacillus licheniformis product are carried out into mixing compounding to obtain
The composite bacteria agent of degraded kitchen garbage.
Embodiment 2
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 25 part, meter
30 parts of 17 parts of aspergillus and bacillus licheniformis.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) 25 parts of Candida lipolyticas, 17 parts of aspergillus oryzaes and 30 parts of bacillus licheniformis are each individually added into kitchen rubbish
In rubbish minimal medium, the culture medium is then positioned over 30 DEG C, 150rmin-1Culture shaking table in carry out domestication training
It is a cycle to support 5d, so repeats to tame after 6 cycles, Candida lipolytica, aspergillus oryzae and lichens gemma bar are obtained respectively
The domestication product of bacterium.
(2) above-mentioned Candida lipolytica, aspergillus oryzae and the domestication of bacillus licheniformis product are carried out into mixing compounding to obtain
The composite bacteria agent of degraded kitchen garbage.
Embodiment 3
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 29 part, meter
28 parts of 21 parts of aspergillus and bacillus licheniformis.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) 29 parts of Candida lipolyticas, 21 parts of aspergillus oryzaes and 28 parts of bacillus licheniformis are each individually added into kitchen rubbish
In rubbish minimal medium, the culture medium is then positioned over 35 DEG C, 200rmin-1Culture shaking table in carry out domestication training
It is a cycle to support 8d, so repeats to tame after 8 cycles, Candida lipolytica, aspergillus oryzae and lichens gemma bar are obtained respectively
The domestication product of bacterium.
(2) above-mentioned Candida lipolytica, aspergillus oryzae and the domestication of bacillus licheniformis product are carried out into mixing compounding to obtain
The composite bacteria agent of degraded kitchen garbage.
Embodiment 4
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 20 part, meter
12 parts of 14 parts of aspergillus, 32 parts of bacillus licheniformis and Trichoderma viride.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) it is 20 parts of Candida lipolyticas, 14 parts of aspergillus oryzaes, 32 parts of bacillus licheniformis and 12 parts of Trichoderma virides are each single
Solely add in kitchen garbage minimal medium, the culture medium is then positioned over 28 DEG C, 120rmin-1 culture shaking table
It is middle carry out domestication culture 4d be a cycle, so repeat tame 4 cycles after, respectively obtain Candida lipolytica, aspergillus oryzae,
Bacillus licheniformis and the domestication product of Trichoderma viride.
(2) the domestication product of above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis and Trichoderma viride is mixed
Compounding obtains the composite bacteria agent of degraded kitchen garbage.
Embodiment 5
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 22 part, meter
15 parts of aspergillus, 31 parts of bacillus licheniformis, 18 parts and 10 parts blown-ball Azotobacters of Trichoderma viride.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) by 22 parts of Candida lipolyticas, 15 parts of aspergillus oryzaes, 31 parts of bacillus licheniformis, 18 parts of Trichoderma virides and 10 parts
Blown-ball Azotobacter is each individually added into kitchen garbage minimal medium, then by the culture medium be positioned over 32 DEG C,
150r·min-1Culture shaking table in carry out domestication culture 5d be a cycle, so repeat tame 5 cycles after, obtain respectively
Candida lipolytica, aspergillus oryzae, bacillus licheniformis, the domestication product of Trichoderma viride and blown-ball Azotobacter.
(2) by the domestication of above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis, Trichoderma viride and blown-ball Azotobacter
Product carries out the composite bacteria agent that mixing compounding obtains degraded kitchen garbage.
Embodiment 6
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 26 part, meter
17 parts of aspergillus, 30 parts of bacillus licheniformis, 22 parts and 15 parts blown-ball Azotobacters of Trichoderma viride.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) by 26 parts of Candida lipolyticas, 17 parts of aspergillus oryzaes, 30 parts of bacillus licheniformis, 22 parts of Trichoderma virides and 15 parts
Blown-ball Azotobacter is each individually added into kitchen garbage minimal medium, then by the culture medium be positioned over 30 DEG C,
180r·min-1Culture shaking table in carry out domestication culture 6d be a cycle, so repeat tame 6 cycles after, obtain respectively
Candida lipolytica, aspergillus oryzae, bacillus licheniformis, the domestication product of Trichoderma viride and blown-ball Azotobacter.
(2) by the domestication of above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis, Trichoderma viride and blown-ball Azotobacter
Product carries out the composite bacteria agent that mixing compounding obtains degraded kitchen garbage.
Embodiment 7
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 29 part, meter
20 parts of aspergillus, 29 parts of bacillus licheniformis, 22 parts and 20 parts blown-ball Azotobacters of Trichoderma viride.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) by 29 parts of Candida lipolyticas, 20 parts of aspergillus oryzaes, 29 parts of bacillus licheniformis, 22 parts of Trichoderma virides and 20 parts
Blown-ball Azotobacter is each individually added into kitchen garbage minimal medium, then by the culture medium be positioned over 35 DEG C,
200r·min-1Culture shaking table in carry out domestication culture 8d be a cycle, so repeat tame 8 cycles after, obtain respectively
Candida lipolytica, aspergillus oryzae, bacillus licheniformis, the domestication product of Trichoderma viride and blown-ball Azotobacter.
(2) by the domestication of above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis, Trichoderma viride and blown-ball Azotobacter
Product carries out the composite bacteria agent that mixing compounding obtains degraded kitchen garbage.
Embodiment 8
A kind of composite bacteria agent for kitchen garbage of degrading, includes the strain of following parts by weight：Candida lipolytica 25 part, meter
20 parts of 15 parts of aspergillus, 30 parts of bacillus licheniformis and Trichoderma viride.
The preparation method of the composite bacteria agent of above-mentioned degraded kitchen garbage, specific preparation process is as follows：
(1) it is 25 parts of Candida lipolyticas, 15 parts of aspergillus oryzaes, 30 parts of bacillus licheniformis and 20 parts of Trichoderma virides are each single
Solely add in kitchen garbage minimal medium, the culture medium is then positioned over 30 DEG C, 150rmin-1Culture shaking table
It is middle carry out domestication culture 5d be a cycle, so repeat tame 5 cycles after, respectively obtain Candida lipolytica, aspergillus oryzae,
Bacillus licheniformis and the domestication product of Trichoderma viride.
(2) the domestication product of above-mentioned Candida lipolytica, aspergillus oryzae, bacillus licheniformis and Trichoderma viride is mixed
Compounding obtains the composite bacteria agent of degraded kitchen garbage.
The composite bacteria agent that above-described embodiment 1~8 is prepared is used for plastics and foam class to being polluted by kitchen garbage
Waste carries out Biological clean, can reach following technical indicator：
1st, Biological clean cycle≤10 day；
2nd, polybag and the foam class waste Biological clean rate of recovery >=85%；
3rd, polybag and foam class waste surface grease residual rate≤5%.
The preferred embodiments of the present invention are above are only, are not intended to limit the invention, for those skilled in the art
For member, inventive formulation and preparation technology can have various modifications and variations.Within the spirit and principles of the invention, institute
Any modification, equivalent substitution and improvements of work etc., should be included in the scope of the protection.
Claims (10)
1. a kind of composite bacteria agent for kitchen garbage of degrading, it is characterised in that：Include the strain of following parts by weight：Solve the false silk ferment of fat
28~33 parts of female 18~29 parts, 12~21 parts of aspergillus oryzae and bacillus licheniformis.
2. the composite bacteria agent of degraded kitchen garbage according to claim 1, it is characterised in that：Also include following parts by weight
Strain：12~26 parts of Trichoderma viride.
3. the composite bacteria agent of degraded kitchen garbage according to claim 1, it is characterised in that：Also include following parts by weight
Strain：10~22 parts of blown-ball Azotobacter.
4. a kind of preparation method of the composite bacteria agent for kitchen garbage of degrading, it is characterised in that：The composite bacteria agent is claim 1
Composite bacteria agent described in~3 any one；The preparation method includes：
Each strain is each added in kitchen garbage minimal medium, then is positioned in culture shaking table by the culture medium
Row domestication culture；
Each strain after the domestication culture be mixed to get to the composite bacteria agent of degraded kitchen garbage.
5. the preparation method of the composite bacteria agent of degraded kitchen garbage according to claim 4, it is characterised in that：The culture
The temperature of shaking table is 25~35 DEG C.
6. the preparation method of the composite bacteria agent of degraded kitchen garbage according to claim 4, it is characterised in that：The culture
The rotating speed of shaking table is 100~200rmin-1。
7. the preparation method of the composite bacteria agent of degraded kitchen garbage according to claim 4, it is characterised in that：The domestication
Incubation time is 3~8d.
8. the preparation method of the composite bacteria agent of degraded kitchen garbage according to claim 4, it is characterised in that：It is described to prepare
Method includes：The domestication incubation is repeated 4~8 times.
9. the clean method of a kind of plastics polluted by kitchen garbage and foam class waste, it is characterised in that：Including：It is biological clear
It is clean；The Biological clean using composite bacteria agent as described in any one of claims 1 to 3 be inoculated into as described in plastics and foam class give up
Biological clean is carried out on thing.
10. the clean method of the plastics according to claim 9 polluted by kitchen garbage and foam class waste, its feature exists
In：The mass ratio of the composite bacteria agent and the plastics and foam class waste is 1:800~1:1200.
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| CN112024575A (en) * | 2020-08-29 | 2020-12-04 | 西南大学 | Method for treating kitchen waste biogas residues by using microbial agent |
| CN112876297A (en) * | 2021-01-31 | 2021-06-01 | 重庆工程职业技术学院 | Method for preparing organic fertilizer by using kitchen garbage |
| CN113278539A (en) * | 2021-03-18 | 2021-08-20 | 惠州市通用机电设备有限公司 | Microbial inoculum for treating organic garbage and preparation and use methods thereof |
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