CN102391876B - Composite biological soil modifier and application thereof - Google Patents
Composite biological soil modifier and application thereof Download PDFInfo
- Publication number
- CN102391876B CN102391876B CN201110292342.1A CN201110292342A CN102391876B CN 102391876 B CN102391876 B CN 102391876B CN 201110292342 A CN201110292342 A CN 201110292342A CN 102391876 B CN102391876 B CN 102391876B
- Authority
- CN
- China
- Prior art keywords
- soil
- bacillus subtilis
- fermentation
- liquid
- trichoderma pseudokiningii
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 239000002689 soil Substances 0.000 title claims abstract description 160
- 239000003607 modifier Substances 0.000 title abstract description 7
- 239000002131 composite material Substances 0.000 title abstract description 4
- 238000000855 fermentation Methods 0.000 claims abstract description 27
- 230000004151 fermentation Effects 0.000 claims abstract description 27
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 25
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 25
- 230000012010 growth Effects 0.000 claims abstract description 18
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000011591 potassium Substances 0.000 claims abstract description 17
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 15
- 241000223259 Trichoderma Species 0.000 claims description 44
- 239000007788 liquid Substances 0.000 claims description 41
- 239000000203 mixture Substances 0.000 claims description 35
- 150000001875 compounds Chemical class 0.000 claims description 32
- 239000000126 substance Substances 0.000 claims description 18
- 244000005700 microbiome Species 0.000 claims description 15
- 230000006872 improvement Effects 0.000 claims description 14
- 230000000050 nutritive effect Effects 0.000 claims description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 239000004615 ingredient Substances 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 229910052698 phosphorus Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 claims description 2
- 229910001950 potassium oxide Inorganic materials 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 29
- 238000012216 screening Methods 0.000 abstract description 9
- 239000005416 organic matter Substances 0.000 abstract description 7
- 229920002101 Chitin Polymers 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 208000035240 Disease Resistance Diseases 0.000 abstract description 2
- 230000002906 microbiologic effect Effects 0.000 abstract description 2
- 230000035699 permeability Effects 0.000 abstract description 2
- 241001304120 Trichoderma pseudokoningii Species 0.000 abstract 3
- 238000009472 formulation Methods 0.000 description 33
- 230000000694 effects Effects 0.000 description 25
- 239000000047 product Substances 0.000 description 20
- 239000003795 chemical substances by application Substances 0.000 description 18
- 238000012360 testing method Methods 0.000 description 17
- 239000003337 fertilizer Substances 0.000 description 14
- 238000002360 preparation method Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 102000004190 Enzymes Human genes 0.000 description 11
- 108090000790 Enzymes Proteins 0.000 description 11
- 229940088598 enzyme Drugs 0.000 description 11
- 241000894006 Bacteria Species 0.000 description 10
- 244000241257 Cucumis melo Species 0.000 description 10
- 238000000034 method Methods 0.000 description 10
- 210000003608 fece Anatomy 0.000 description 9
- 230000035558 fertility Effects 0.000 description 9
- 239000010871 livestock manure Substances 0.000 description 9
- 240000002319 Citrus sinensis Species 0.000 description 8
- 230000015556 catabolic process Effects 0.000 description 8
- 238000006731 degradation reaction Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 8
- 235000005976 Citrus sinensis Nutrition 0.000 description 7
- 238000005056 compaction Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 238000004321 preservation Methods 0.000 description 7
- 238000004162 soil erosion Methods 0.000 description 7
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 6
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000004016 soil organic matter Substances 0.000 description 6
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 5
- 241000223261 Trichoderma viride Species 0.000 description 5
- 239000013543 active substance Substances 0.000 description 5
- 201000010099 disease Diseases 0.000 description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000002703 mutagenesis Methods 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 239000007921 spray Substances 0.000 description 5
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 102000035195 Peptidases Human genes 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- 235000009508 confectionery Nutrition 0.000 description 4
- 230000008635 plant growth Effects 0.000 description 4
- 239000002028 Biomass Substances 0.000 description 3
- 108010059892 Cellulase Proteins 0.000 description 3
- 108010022172 Chitinases Proteins 0.000 description 3
- 102000012286 Chitinases Human genes 0.000 description 3
- 241000726221 Gemma Species 0.000 description 3
- 101710184309 Probable sucrose-6-phosphate hydrolase Proteins 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 102400000472 Sucrase Human genes 0.000 description 3
- 101710112652 Sucrose-6-phosphate hydrolase Proteins 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 229940106157 cellulase Drugs 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 235000011073 invertase Nutrition 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 235000021049 nutrient content Nutrition 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 2
- 108010013043 Acetylesterase Proteins 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- AFWTZXXDGQBIKW-UHFFFAOYSA-N C14 surfactin Natural products CCCCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 AFWTZXXDGQBIKW-UHFFFAOYSA-N 0.000 description 2
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 2
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 2
- 240000008067 Cucumis sativus Species 0.000 description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 2
- 241000223224 Fusarium oxysporum f. cucumerinum Species 0.000 description 2
- 241000508192 Fusarium oxysporum f. sp. niveum Species 0.000 description 2
- 229920001503 Glucan Polymers 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000004157 Hydrolases Human genes 0.000 description 2
- 108090000604 Hydrolases Proteins 0.000 description 2
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 2
- 229940074393 chlorogenic acid Drugs 0.000 description 2
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 2
- 235000001368 chlorogenic acid Nutrition 0.000 description 2
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 2
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000000378 dietary effect Effects 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 238000003760 magnetic stirring Methods 0.000 description 2
- 239000002075 main ingredient Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 239000011707 mineral Substances 0.000 description 2
- 235000010755 mineral Nutrition 0.000 description 2
- 239000002420 orchard Substances 0.000 description 2
- 230000020477 pH reduction Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 229920002451 polyvinyl alcohol Polymers 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 230000002786 root growth Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000012807 shake-flask culturing Methods 0.000 description 2
- 239000002688 soil aggregate Substances 0.000 description 2
- -1 specifically Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- NJGWOFRZMQRKHT-UHFFFAOYSA-N surfactin Natural products CC(C)CCCCCCCCCC1CC(=O)NC(CCC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)C)C(=O)NC(CC(O)=O)C(=O)NC(CC(C)C)C(=O)NC(CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-UHFFFAOYSA-N 0.000 description 2
- NJGWOFRZMQRKHT-WGVNQGGSSA-N surfactin C Chemical compound CC(C)CCCCCCCCC[C@@H]1CC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)O1 NJGWOFRZMQRKHT-WGVNQGGSSA-N 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- 101710130006 Beta-glucanase Proteins 0.000 description 1
- 102000003712 Complement factor B Human genes 0.000 description 1
- 108090000056 Complement factor B Proteins 0.000 description 1
- 108010001682 Dextranase Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102100032352 Leukemia inhibitory factor Human genes 0.000 description 1
- 108090000581 Leukemia inhibitory factor Proteins 0.000 description 1
- 108010028921 Lipopeptides Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- 229920001807 Urea-formaldehyde Polymers 0.000 description 1
- 108010046334 Urease Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 238000004177 carbon cycle Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 230000028023 exocytosis Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 239000010881 fly ash Substances 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 229940084434 fungoid Drugs 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000009332 manuring Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000000618 nitrogen fertilizer Substances 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000000243 photosynthetic effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses an unpolluted, green and high-efficiency composite biological soil modifier which comprises the following components: trichoderma pseudokoningii fermentation liquor, bacillus subtilis fermentation liquor, water-soluble chitin, potassium humate and decomposed organic matter. The trichoderma pseudokoningii and the bacillus subtilis are high-efficiency bacterial strains which are obtained through screening and mutating, the trichoderma pseudokoningii fermentation liquor and the bacillus subtilis fermentation liquor are obtained by a liquor submerged fermentation technology, and the unpolluted, green and high-efficiency composite biological soil modifier can be prepared in a combination way. The soil modifier has the following functions that the physicochemical property of the soil can be improved, the hardening of the soil can be broken up, the volume weight of the soil can be reduced, the air permeability of the soil can be improved, the microbiological activity of the soil can be promoted, the growth of the plant root system can be promoted, the disease resistance of the crop can be enhanced, the yield of the crop can be improved, the quality of the crop can be improved, the original ecology of the crop can be recovered and the like, and the soil modifier is the novel soil modifier which is high-efficiency, environment-friendly and convenient to use.
Description
Technical field
The present invention is a kind of compound soil modifier, specifically, potassium fulvate and decomposed organic matter is added centered by active composite bacteria, both soil physico-chemical property can be improved, there is provided crop growth necessary nutritive substance, can suppress Plant diseases again, be a kind of soil improvement agent of green high-efficient.
Background technology
Soil is the important carrier of plant growth environment, and it provides photosynthetic raw material and attaching substratum to plant, soil quality and crop yield closely bound up.Soil quality refers to that soil has animals and plants production persistence in the ecosystem boundary of nature or labor management, keeps and improve the ability of water, makings amount and human health and life.The basis of soil quality is soil fertility, and soil fertility is the ability that soil provided for plant-growth and coordinate nutritional condition and envrionment conditions, is the general performance of the various essential property of soil.The measure of increasing soil fertility comprises: applying organic manure material, green manuring and rational use of chemical fertilizer, and these measures are not only conducive to the high yield of this season crop, and is conducive to recovery and the raising of soil fertility.Current, the Soil degradation problem caused due to various irrational mankind's activity (depend on unduly and irrational use chemical fertilizer, excessively cultivation, single cropping system etc.) and natural cause serious threat, to agriculture sustainable development, causes Soil degradation.Soil degradation can be divided into physical degradation, chemical degradation and biodeterioration three types, and main manifestations is that the soil erosion, the soil salinization and compacting are hardened, soil acidification, the soil organism run off, heavy metal pollution of soil poisons.Current, the main forms of China's Soil degradation is the soil erosion, soil compaction.According to statistics, China's soil erosion area in 1996 reaches 1,830,000 surprising km
2, account for 19.00% of area, wherein southern red yellow earth Soils In The Region erosion area is 6.15 × 10
5km
2, account for 25.00% of soil, this area total area.South Red Soil Region now about has 2 × 10
5km
2soil there is serious acidifying situation.The soil erosion, soil compaction have had a strong impact on the development of China's agriculture production.
The soil erosion is also referred to as soil erosion, refer to that topsoil or soil parent material are under the effects such as water, wind, gravity, the various forms of phenomenon of degrading, carrying and piling up occurring, causes topsoil loss, soil desertification, soil compaction compacting, is the most important factor affecting Soil degradation.The vegetation deterioration that human activity causes and soil bareness are the major reasons of the aggravation soil erosion.
Soil compaction is that Soil structure destroys under rainfall or the external cause action such as to pour water, the phenomenon of poly-compacting in after dry.Applying excessive nitrogenous fertilizer in soil, cause the soil organic substance that microbial consumption is too much, affect microorganism active, spoiled soil aggregate structure, is the major reason of soil compaction.
From 19 end of the centurys so far, in order to solve Soil degradation problem, scientific worker's door of country variant develops multiple soil improvement agent.Natural amendments, synthetic modifying agent and biological modification agent can be divided into according to its raw material sources.Natural amendments, as natural mineral, inorganic and organic solid castoff, extraction high molecular polymer.Synthesis modifying agent is the high molecular polymer of the structure and function synthetic of simulation natural amendments, as polyacrylamide, polyvinyl alcohol resin, polyvinyl alcohol, urea-formaldehyde resin etc.In production application, natural amendments and synthesis modifying agent also exist some theoretical and practical problemss and negative effect.As: flyash has stronger absorption fixed action to P; Organic waste contains the objectionable impuritiess such as various pathogenic bacteria, heavy metal, polychlorobiphenyl; The production cost of polyacrylamide is higher and intermediate product acrylamide that is its degraded is poisonous etc.
The biological modification agent of current China of a great variety, mixed and disorderly, but mostly be extensive routine techniques, bacterial classification and formula are without specific aim, the effective constituent standard of product is chaotic, quality product and practical application effect undesirable, and seriously polluted in production process, large-scale promotion application example is less.The separation screening of high-efficiency strain, purifying cultivate problem, production cost and Environmental capacity and environmental safety assessment is still the bottleneck that the agent of restriction biological modification develops.
Summary of the invention
For Soil degradation and the problem such as existing soil improvement agent effect is not good, the object of the present invention is to provide a kind of pollution-free, efficient, practical compound biological soil improver, present invention also offers the application method of this soil improvement agent.
The component of compound biological soil improver of the present invention comprises trichoderma pseudokiningii fermented liquid and fermentation of bacillus subtilis liquid.
Compound biological soil improver of the present invention, comprises following main ingredient:
Trichoderma pseudokiningii fermented liquid
Fermentation of bacillus subtilis liquid
N,O-CMC
Potassium fulvate
The trichoderma pseudokiningii fermented liquid used in compound biological soil improver of the present invention, be by trichoderma pseudokiningii (
trichoderma pseudokiningii) TRIPI fermentation obtains.
Trichoderma pseudokiningii selected by the present invention (
trichoderma pseudokiningii) TRIPI be from stalk be separated, then through the novel strain of mutagenesis, screening, called after trichoderma pseudokiningii (
trichoderma pseudokiningii) TRIPI.This bacterial strain carries out preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, preserving number is CGMCC NO.1443, the preservation time is on August 22nd, 2005, and preservation address is No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica.
The present inventor will from the stalk of experimental plot isolated trichoderma pseudokiningii original strain in the usual way, through ultraviolet secondary multiple mutated (1 × 10
6the bacteria suspension of individual/ml is put in the culture dish of diameter 9 cm, ultraviolet lamp preheat 20 min, by culture dish as magnetic stirring apparatus makes culture dish at 10W ultraviolet lamp vertical range 25 about cm, irradiate 90S), filter out the bacterial strain with higher mycelial growth rate, cellulase activity and bacteriostatic activity, be trichoderma pseudokiningii that preserving number provided by the present invention is CGMCC NO.1443 (
trichoderma pseudokiningii) TRIPI.
Trichoderma pseudokiningii of the present invention (
trichoderma pseudokiningii) cultural method of TRIPI is:
1. with transfering loop by cryopreserved trichoderma pseudokiningii TRIPI strain inoculation in dull and stereotyped PDA substratum (potato 200g, sucrose 20g, agar 20g, distilled water 1000mL), transferred for 2 generations; 2. transfering loop picking edge mycelia is transferred in PDB substratum (potato 200g, sucrose 20g, distilled water 1000mL) in, culture temperature 25 DEG C ~ 30 DEG C, optimum temperuture is 28 DEG C, 150rpm, and the time is 7 ~ 10 days, fermented liquid 100 μm of sieved filters obtain spore suspension, and spore concentration of living is 3-9 × 10
8individual/ml.
This bacterial strain filtered out was transferred for 10 generations continuously, its good character inheritance stability.
Table 1 trichoderma pseudokiningii mutagenesis results
In the active and potted plant experiment of starting strain and mutant strain shake-flask culture dry mycelial weight and fermented liquid (1000r/min, centrifugal 10min) Filter paperlyase (FPA) after 5 days, endo-type-beta-glucanase (C1 enzyme), circumscribed-type-beta-glucanase (CBH enzyme) to cucumber verticillium wilt pathogen (
fusarium oxysporum f.sp. cucumerinum), withered germ of water-melon (
fusarium oxysporum f.sp. niveum) prevention effect.
。
The fermentation of bacillus subtilis liquid used in compound biological soil improver of the present invention, be by subtilis (
bacillus subtilis) BACI fermentation obtains.
Subtilis in the present invention (
bacillus subtilis) BACI be from plant rhizosphere soil be separated, then through the novel strain of mutagenesis, screening, called after subtilis (
bacillus subtilis) BACI.This bacterial strain carries out preservation at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC, preserving number is CGMCC NO.1442, the preservation time is on August 22nd, 2005, and preservation address is No. 13, North No.1 Row, Zhongguancun, Haidian District, Beijing City, Institute of Microorganism, Academia Sinica.
The present inventor will from the plant rhizosphere soil of experimental plot isolated subtilis original strain in the usual way, through ultraviolet secondary complex mutation (1 × 10
7the bacteria suspension of individual/ml is put in the culture dish of diameter 9 cm, ultraviolet lamp preheat 20 min, by culture dish as magnetic stirring apparatus makes culture dish at 20 W ultraviolet lamp vertical range 30 about cm, irradiate 150S) filter out the bacterial strain with higher growth rate, 'beta '-mannase, beta glucan enzyme, proteolytic enzyme and bacteriostatic activity, be subtilis that preserving number provided by the present invention is CGMCC NO.1442 (
bacillus subtilis) BACI.
Subtilis in the present invention (
bacillus subtilis) cultural method of BACI is: the mode adopting liquid submerged fermentation to cultivate is carried out fermentation culture and is obtained bacillus subtilis spore
1. by the subtilis of preservation (
bacillus subtilis) BACI bacterial classification, streak inoculation is in beef-protein medium (extractum carnis 0.3g, peptone 1g, sodium-chlor 0.5g, agar 2g, distilled water 100ml, pH7.0 ~ 7.2) activation 24h; 2. transfering loop picking thalline is inoculated in LB substratum (tryptone 10g, yeast extract 5g, NaCl 10g, distilled water 1000ml), enlarged culturing 24h; 3. (potato 200g, glucose 20g, distilled water 1000ml) in PDB substratum is inoculated in 1% inoculum size, fermentation condition is: temperature 37 DEG C, pH=7, and dissolved oxygen concentration maintains 20-30% saturation ratio, 220rpm, incubation time is 2 ~ 3 days, and effective cell concentration is 1-3 × 10
9individual/ml.
This bacterial strain filtered out was transferred for 10 generations continuously, its good character inheritance stability.
Table 2 subtilis mutagenesis results
After starting strain and mutant strain shake-flask culture 24h, in fermented liquid (8000r/min, centrifugal 20min) 'beta '-mannase, beta-glucanase, protease activity and potted plant experiment to cucumber verticillium wilt pathogen (
fusarium oxysporum f.sp. cucumerinum), withered germ of water-melon (
fusarium oxysporum f.sp. niveum) prevention effect.
。
Trichoderma pseudokiningii a kind ofly has very much the bacterial strain that soil improves potentiality, and widely distributed, growth potential is vigorous.It is varied that its soil improves mechanism.Trichoderma pseudokiningii has abundant mycelia, can adhere to and hand over around soil particle, improves soil stability and permeability, breaks soil compaction; Trichoderma pseudokiningii also can high expression β-1, the highly active specific enzymes systems such as 4-glucan hydrolase, chitinase, cellulase, proteolytic enzyme, the outer macromolecule polysaccharide of born of the same parents that secretion is abundant and glycoprotein, soil physico-chemical property can be improved, the release of promotion soil nutrient and root system of plant, to the absorption of nutritive ingredient, promote plant root growth; Produce the active substance of volatility or non-volatile suppression growth of pathogenic bacteria, as sticky broom toxin, coal Buddhist nun toxin and victory peptide element etc. suppress harmful microbe growth, β-1,4-glucan hydrolase and the cell walls of chitinase to plant pathogenic fungi have strong hydrolytic action, cause the disintegration of pathogenic agent and suppress the sprouting of pathogenic bacteria spore and gemma.In addition, trichoderma pseudokiningii adaptability is extremely strong, all can grow fast between 4 DEG C ~ 42 DEG C He under multiple edatope, can capture site, space and nutritional resource fast.
Subtilis, its resistance under gemma state is extremely strong, can the various inverse property environment such as resistance to oxidation, high temperature.Subtilis can produce the abundant physiologically active substance with high vigor and control of plant disease function in process of growth, comprise proteolytic enzyme, amylase, cellulase, dextranase, polygalacturonase, VITAMIN, chitinase, amino acid, somatomedin, lipopeptide antibiotic, iturin, the multiple compounds such as surfactin, they have promotion decomposition of soil organic matter, improve the fertile ability of soil control and soil enzyme activities, improve Soil Microorganism Bacterial community, promote plant root growth, suppress harmful fungoid, bacterium, the effects such as virus and bacterium substance, wherein Surfactin has inhibit activities to various plants pathogenic bacteria.Its meta-bolites strong stress resistance, can play effectiveness for a long time, reduces consumption, both can increase soil fertility, and promotes plant strain growth, can prevent disease again, improves crop yield.
Trichoderma pseudokiningii and subtilis all can secrete the active substance that exocellular polysaccharide, organic acid, glycoprotein, VITAMIN, specific enzymes system etc. have certain physiological function in process of growth, because the morphological properties of two kinds of bacterial classifications is different, thus, improving soil physico-chemical property, promote the growth of root system of plant, strengthen root system to the receptivity of nutritive ingredient, regulate rhizosphere soil microorganism Bacterial community many-sided, complementation and enhancement can be played.And a large amount of trichoderma pseudokiningii mycelia can firmly adhere to soil particle and be wound around, improve soil stability and porosity.
Chitin has " plant vaccine ", uses continuously and can induce the resistance factor of farm crop self generation to disease, reduce disease incidence, significantly reduce Pesticide use amount.In addition it also has extremely strong blade face adhesion property, can urge root strong sprout, promotes fruit maturation.
Potassium fulvate has high capacity amount and physiologically active, and it is plant utilization that its chelating constant and trace nutrient can make it better, excites plant microbiological active, can slow release fertilizer, improve the absorption of Plant To Nutrient, promote plant germination and growth, accelerate precipitation to decompose, strengthen Flood resistance.
Chitin and potassium fulvate carry out chelating with its different structural form, play mutual promoting action, are more conducive to the improvement of Soil structure.
In order to special effect of comprehensive and screening trichoderma pseudokiningii, subtilis, chitin, potassium fulvate and mutual synergism and best complex proportioning, the present inventor adopts orthonormal design of experiments, is optimized the quality proportioning number of component each in described compound biological soil improver.
Supplying to study thing is Japanese sweet treasured, and seed is produced by the sweet precious seed produces base of Qingdao Japan, and experiment location, field is positioned at Zhaoyuan City, and orthogonal and experimental result are in table 3, table 4.
The table 3 orthogonal experiment factor and horizontal L
9(3
4)
Table 4 Orthogonal experiment results L
9(3
4)
| Experiment number | A factor | B factor | C factor | D factor | The soil weight (g/cm 3） | Melon yield (kg/ mu) | Active soil organic matter (g/kg) |
| 1 | 1 | 1 | 1 | 1 | 1.55 | 3190 | 5.6 |
| 2 | 1 | 2 | 2 | 2 | 1.58 | 3005 | 6.3 |
| 3 | 2 | 1 | 2 | 3 | 1.51 | 3299 | 6.4 |
| 4 | 2 | 2 | 3 | 1 | 1.49 | 3364 | 7.1 |
| 5 | 2 | 3 | 1 | 2 | 1.50 | 3308 | 6.9 |
| 6 | 3 | 1 | 3 | 2 | 1.48 | 3469 | 7.5 |
| 7 | 3 | 2 | 1 | 3 | 1.46 | 3371 | 8.2 |
| 8 | 1 | 3 | 3 | 3 | 1.50 | 3505 | 7.3 |
| 9 | 3 | 3 | 2 | 1 | 1.43 | 3672 | 8.6 |
| Soil weight K1 | 1.47 | 1.51 | 1.50 | 1.51 | |||
| K2 | 1.54 | 1.52 | 1.50 | 1.49 | |||
| K3 | 1.49 | 1.46 | 1.50 | 1.50 | |||
| Extreme difference R | 0.07 | 0.06 | 0.01 | 0.02 | |||
| Melon yield k1 | 3483.00 | 3287.33 | 3370.67 | 3228.00 | |||
| k2 | 3233.33 | 3257.67 | 3343.33 | 3446.00 | |||
| k3 | 3344.67 | 3516.00 | 3347.00 | 3387.00 | |||
| Extreme difference R | 249.67 | 258.33 | 27.33 | 218.00 | |||
| Active soil organic matter k1 | 7.67 | 6.53 | 6.87 | 7.13 | |||
| k2 | 6.40 | 6.73 | 7.10 | 7.30 | |||
| k3 | 7.23 | 8.03 | 7.33 | 6.87 | |||
| Extreme difference R | 1.27 | 1.50 | 0.47 | 0.43 |
As can be seen from land for growing field crops Orthogonal experiment results, four kinds of components all have an impact to soil property and crop yield.Each factor pair soil weight influence order is successively: A > B > D > C; And to melon yield influence order successively: B > A > D > C; And to active soil organic matter content influence sequence of operation successively: A > B > C > D.In visible compound preparation, trichoderma pseudokiningii and subtilis play decisive role to soil property and crop yield, trichoderma pseudokiningii content is the most remarkable to the soil weight and active soil organic matter content influence, and subtilis is then remarkable on the impact of melon yield.Can be found to take the soil weight as screening target or take active soil organic matter as screening target by result, or be screening target with melon yield, No. 9 level of factor A
3b
3c
2d
1, be the optimal proportion under this experiment condition.
According to the result of orthogonal test, and take into account processing and the many factors in using, the present inventor determines, in compound biological soil improver of the present invention, the parts by weight of each main ingredient are as follows simultaneously:
Trichoderma pseudokiningii fermented liquid 38-42 part, fermentation of bacillus subtilis liquid 38-42 part, N,O-CMC 0.5-1.5 part, potassium fulvate 0.5-1.5 part;
Wherein, effective spore concentration>=3 × 10 in trichoderma pseudokiningii fermented liquid
8individual/ml; Fermentation of bacillus subtilis liquid gemma number>=1 × 10
9individual/ml; N,O-CMC: molecular weight M is about (1-5) × 10
5, viscosity 100-200mPa, de-ethyl ester degree DAC>=90%, purity>=90%; Potassium fulvate specification is: fineness < 120 order, water-soluble substance > 99.7%, water-soluble xanthohumic acid > 50%, potassium oxide 9.5-10%, nitrogen 2.5-3.0%, phosphorus 0.5-0.8%, organic 50-60%.(wherein percentage composition is % by weight)
For improving this preparation at the germination rate using initial stage trichoderma pseudokiningii spore and subtilis, can suitably add conventional decomposed organic matter, its interpolation weight is 5 ~ 20 parts and is advisable.
In above-mentioned weight consumption proportional range, compound biological soil improver of the present invention all can obtain comparatively ideal soil improvement and protected effect.
The preparation method of compound biological soil improver of the present invention is as follows:
By required parts by weight, trichoderma pseudokiningii spore and fermentation of bacillus subtilis liquid are mixed, then N,O-CMC is evenly added in bacterium liquid and dissolve, again potassium fulvate is added in mixed solution, be uniformly mixed, be processed as stable liquid compound biological soil improver.The chlorogenic acid isoreactivity material that also can add marine bacteria meta-bolites simultaneously and extract from halophytes.
Compound biological soil improver consumption of the present invention is: new stub land 500-1500ml/ mu, old stub land 1500-2500ml/ mu.
Embody rule mode is: by liquid soil improvement dilution agent 100-200 doubly, before field planting, turns over or fill with root after evenly spraying soil surface.
Compound biological soil improver of the present invention is applicable to the improvement of the multiple soil such as rice soil, hydromorphic soil, pedocal, alfisol, saline-alkali soil.
Compound biological soil improver of the present invention is subject to the favor of more and more agricultural science and technology worker with its efficient soil improvement ability and crop yield ability, and microorganism species is wherein important function ingredients.Soil microorganisms is circulated at the biochemistry of Zinc fractions, the ecosystem, have vital role in the degraded of extraneous contamination material and plant stress-resistance etc., the physico-chemical property of soil can be improved, promote the formation of soil aggregate and stablize, mineral nutrient in activating soil, effective improvement Soil Micro-environment, strengthens dietetic alimentation ability and its disease resistance, resistance etc. of plant.，
Compound biological soil improver of the present invention all has obvious production-increasing function to various crop such as muskmelon, wheat, navel oranges.
The present invention is component centered by the novel trichoderma pseudokiningii of separation, mutagenesis, screening and subtilis, and add chitin, potassium fulvate and decomposed organic matter, be intended to the physico-chemical property by improving soil, break soil compaction and compacting, promote the decomposition of the soil organism and the release of nutritive ingredient, improve the fertile ability of soil control, regulate soil pH, regulate microbial bacteria group structure, suppress harmful microbe growth, strengthen root system of plant and the receptivity of nutritive substance is promoted that it grow, reach improvement soil and increase production object.The two kinds of bacterial strain reproduction speeds used in the present invention are very fast, have high exocytosis active, utilize liquid submerged fermentation technology to achieve large-scale production; a large amount of thalline and spore can be obtained fast; reduce production cost, and product is liquid preparation, easy to use.
Embodiment
One. soil improvement agent liquid preparation amounts of components embodiment of the present invention is as follows:
Example of formulations 1
Component and parts by weight:
Trichoderma pseudokiningii fermented liquid 38 parts, fermentation of bacillus subtilis liquid 42 parts, N,O-CMC 0.5 part, potassium fulvate 1 part, decomposed organic matter 10 parts, chlorogenic acid isoreactivity agent 5 parts, steady quality agent 3.5 parts.
Liquid compound biological soil improver is processed as by aforementioned preparation process.
Example of formulations 2
Component and parts by weight:
Trichoderma pseudokiningii fermented liquid 40 parts, fermentation of bacillus subtilis liquid 40 parts, N,O-CMC 1.0 parts, potassium fulvate 1 part, decomposed organic matter 10 parts, 4.5 parts, tensio-active agent, steady quality agent 3.5 parts.
Liquid compound biological soil improver is processed as by aforementioned preparation process.
Example of formulations 3
Component and parts by weight:
Trichoderma pseudokiningii fermented liquid 42 parts, fermentation of bacillus subtilis liquid 38 parts, N,O-CMC 1.5 parts, potassium fulvate 0.5 part, decomposed organic matter 10 parts, 4.5 parts, tensio-active agent, steady quality agent 3.5 parts;
Liquid compound biological soil improver is processed as by aforementioned preparation process.
Two. soil improvement agent Comparison study test example of the present invention is as follows:
Test example 1
Application invention formulation embodiment 1, compared with single Trichoderma preparation, on the impact (Zhaoyuan, shandong) of Jimai 22 output and the soil weight, pH
Test group 1 is use this product formulation embodiment 1 of 1000ml/ mu, test group 2 is for using the single Trichoderma preparation containing identical effective spore quantity of 1000ml/ mu, evenly spray in soil surface before turning over, control group sprays equivalent clear water, follow-up process of topdressing is identical, plot area 100 ㎡, measure and calculation soil physico-chemical property and wheat yield after harvesting wheat.Result is as shown in table 5, decline relative to the control group soil weight after using this product formulation embodiment 1 and viride bacteria preparation, pH value is close to neutral, soil acidification situation is reversed, be suitable for plant growth, wheat yield increases, and the effect of this product formulation embodiment 1 is better than single Trichoderma preparation.
Table 5 invention formulation embodiment 1 compared with Trichoderma preparation, on the impact of soil physico-chemical property
Test example 2
Invention formulation embodiment 2 and existing trichoderma viride, subtilis compound formulation are on the impact (Zhaoyuan, shandong) of muskmelon soil for growth nutrient
Soil nutrient is the important symbol of soil fertility, is the basis of plant growth.Supplying to study thing is Japanese sweet treasured, and seed is produced by the sweet precious seed produces base of Qingdao Japan.Test group is get 1000ml/ mu invention formulation embodiment 2 before field planting to be sprayed on soil surface, and control group is trichoderma viride and the subtilis compound formulation of the identical effective thalline quantity using 1000ml/ mu, turns over.Result is as shown in table 6, and invention formulation embodiment 2 and control group process are improved to nutrient content in soil, facilitate the release of soil nutrient, and effect of invention formulation embodiment 2 is better than existing trichoderma viride and subtilis compound formulation.
This product formulation of table 6 embodiment 2 and existing viride, subtilis compound formulation are on the impact of melon yield and soil nutrient content
Test example 3 product formulation embodiments 2 and existing viride, subtilis compound formulation are on the impact (Zhaoyuan, shandong) of muskmelon Rhizospheric microorganisms' quantity
Test group 1 is the invention formulation embodiment 2 that before field planting, soil surface sprays 1000ml/ mu, and test group 2 sprays existing trichoderma viride and the subtilis compound formulation that equivalent has identical effective thalline quantity, and control group sprays equivalent clear water.Microorganism in soil not only can regulate plant strain growth, and has vital role in the maintenance of soil fertility and raising and Conversion of energy and material cycle etc., is the important indicator of soil quality.Result is as shown in table 7, trichoderma viride and the subtilis compound formulation of this product formulation embodiment 2 and identical effective thalline quantity all improve Soil Microorganism quantity compared to control group process, add microbial diversity, and the quantity of this product formulation embodiment 2 pairs of bacteriums and fungi promotes the most remarkable, apparently higher than test group 2.
Table 7 viride, subtilis compound formulation and this product formulation embodiment 2 are on the impact of muskmelon Rhizospheric microorganisms' quantity
Test example 4
This product formulation embodiment 2 and commercially available microbial-bacterial fertilizer are on the impact (Xinfeng, Jiangxi) of 20 years Planting Years Jiangxi south navel orange output and orchard Underground roots biomass
Test group 1: require to specifications flower early stage, strong fruit phase respectively every strain use commercially available microbial-bacterial fertilizer (central. set is divided into trace element and active subtilis) 1kg, test group 2: the embodiment of the present invention 2 liquid recombiner 100ml is used in every strain, ditching landfill or water within the scope of navel orange plant trunk radius 50cm before plant is sprouted respectively, control group is equivalent clear water, and follow-up management mode is identical.After fruit harvest, in institute's sampling ground, random selecting 3 standard navel orange trees, collect 0-45cm soil layer root system, the features such as the complexity that the diameter of foundation root, profile, color, elasticity, Gen Pi are separated with center pillar separate by heavy manual labour root (>2mm), middle root alive (0.5-2mm), fine work root (<0.5mm), dead root four kinds of situations respectively, then repeatedly clean with distilled water, dry at 85 DEG C to constant weight, claim its quality, be finally scaled biomass in unit area (gm
-2).Plant fine root is the most important part absorbing nutritive substance in root system, and their content is also the important indicator evaluating nutrition status of the plant.Use commercially available microbial-bacterial fertilizer and this product formulation embodiment 2 to be as can be seen from Table 8 significantly increased radicula content in navel orange root system, dead radical content also significantly declines, and facilitate the growth of the newborn root system of plant, crop yield improves.Fine work root, as component most important in root system, more can reflect the dietetic alimentation ability of plant, and this product formulation embodiment 2 promotes that the growth of navel orange fine work root and the raising effect of output are better than commercially available microbial-bacterial fertilizer.
This product formulation of table 8 embodiment 2 and commercially available microbial-bacterial fertilizer are on the impact of 20 years Planting Years Jiangxi south navel orange orchard Underground roots biomass and navel orange output
Test example 5
This product formulation embodiment 3 and commercially available microbial-bacterial fertilizer and farm manure are on the impact (Zhaoyuan, shandong) of black fallow soil enzyme activities
Test group 1: use commercially available microbial-bacterial fertilizer 100kg/ mu, test group 2: use this product 1000ml/ mu, control group: applications of farmyard manure (hog manure) 500kg/ mu, turns over after using and rakes, measure soil enzyme activities after 6 months.The height of soil enzyme activities is closely related with soil physico-chemical property (comprising Soil structure, organic content and soil nutrient content etc.) and Soil Microorganism amount, especially very close with the relation of soil fertility.Sucrase, Phosphoric acid esterase, urase three kinds of enzyme activities characterize carbon cycle in dynamic changes of soil process, the conversion of soil phosphorus element and utilization, the conversion of Soil Nitrogen element and utilization respectively.As can be seen from Table 9, after using commercially available microbial-bacterial fertilizer, farm manure (hog manure) and this product, in soil, the vigor of sucrase, Phosphoric acid esterase and urase three kinds of enzymes all has lifting, and display biological activity of soil improves, and soil nutrient release is accelerated, restoration of soil fertility.The raising of this product formulation embodiment 3 pairs of sucrases and urease activity is better than commercially available microbial-bacterial fertilizer and farm manure, suitable with commercially available microbial-bacterial fertilizer on the impact of phosphatase activity, higher than farm manure.
This product formulation of table 9 embodiment 3 and commercially available microbial-bacterial fertilizer and farm manure are on the impact of black fallow soil enzyme activities
Claims (3)
1. a compound biological soil improver, is characterized in that composed of the following components: fermentation of bacillus subtilis liquid, trichoderma pseudokiningii fermented liquid, N,O-CMC, potassium fulvate; The quality proportioning number of each component is: fermentation of bacillus subtilis liquid 38-42 part, trichoderma pseudokiningii fermented liquid 38-42 part, N,O-CMC 0.5-1.5 part, potassium fulvate 0.5-1.5 part;
Described fermentation of bacillus subtilis liquid be by preserving number be CGMCC NO.1442 subtilis (
bacillus subtilis) BACI fermentation obtains, fermentation of bacillus subtilis liquid viable count>=1 × 10
9individual/ml;
Described trichoderma pseudokiningii fermented liquid be by preserving number be CGMCC NO.1443 trichoderma pseudokiningii (
trichoderma pseudokiningii) TRIPI fermentation obtains, effective spore concentration>=3 × 10 in trichoderma pseudokiningii fermented liquid
8individual/ml;
Described N,O-CMC molecular weight M is (1-5) × 10
5, viscosity is 100-200mPa, de-ethyl ester degree DAC>=90%, purity>=90%;
Described potassium fulvate specification is: fineness < 120 order, water-soluble substance > 99.7%, water-soluble xanthohumic acid > 50%, potassium oxide 9.5-10%, nitrogen 2.5-3.0%, phosphorus 0.5-0.8%, organic 50-60%, wherein percentage composition is % by weight.
2. the compound biological soil improver as described in claim 1, is characterized in that described each constituent mass number is: fermentation of bacillus subtilis liquid 40 parts, trichoderma pseudokiningii fermented liquid 40 parts, N,O-CMC 1 part, potassium fulvate 1 part.
3. the application of compound biological soil improver as claimed in claim 1 or 2; it is characterized in that for improvement and the soil quality that protects the fields---improve soil physico-chemical property; regulate rhizosphere soil microorganism Bacterial community; promote the growth of root system of plant; strengthen root system to the receptivity of nutritive ingredient; improve soil stability and porosity, accelerate precipitation and decompose, improve Soil structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110292342.1A CN102391876B (en) | 2011-09-29 | 2011-09-29 | Composite biological soil modifier and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110292342.1A CN102391876B (en) | 2011-09-29 | 2011-09-29 | Composite biological soil modifier and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102391876A CN102391876A (en) | 2012-03-28 |
| CN102391876B true CN102391876B (en) | 2015-03-04 |
Family
ID=45859262
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110292342.1A Expired - Fee Related CN102391876B (en) | 2011-09-29 | 2011-09-29 | Composite biological soil modifier and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102391876B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713235A (en) * | 2020-05-29 | 2020-09-29 | 金正生物化工有限公司 | High-quality high-yield fertilization method for sweet potato continuous cropping planting |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103146617A (en) * | 2013-03-18 | 2013-06-12 | 济南巨微生物技术有限公司 | Bacillus subtilis and culture method and application thereof |
| CN103804101B (en) * | 2014-01-27 | 2015-11-25 | 威海索尔生物科技有限公司 | A kind of method using Chitosan powder soil improvement agent to improve soil |
| CN104108964B (en) * | 2014-07-08 | 2016-06-15 | 山东大学 | The preparation method of a kind of full vegetative microorganism biological and ecological methods to prevent plant disease, pests, and erosion fertilizer and application |
| CN105964680B (en) * | 2016-05-06 | 2019-04-09 | 山东省农业科学院农产品研究所 | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof |
| CN105918362A (en) * | 2016-06-05 | 2016-09-07 | 河南科技学院 | Composition for preventing plant diseases |
| CN106417386A (en) * | 2016-09-22 | 2017-02-22 | 黑龙江田力保生物科技有限公司 | Leaf type wettable biological agent and use method thereof |
| CN107231982A (en) * | 2017-06-16 | 2017-10-10 | 王化兰 | Plant protection method and application |
| CN107840688A (en) * | 2017-09-29 | 2018-03-27 | 北京市农业环境监测站 | Purposes of the composite biological agent in fruits and vegetables class product antistaling agent is prepared |
| CN107663118A (en) * | 2017-09-29 | 2018-02-06 | 北京市农业环境监测站 | A kind of purposes of composite biological agent in fruits and vegetables class product quality is improved |
| CN107690863A (en) * | 2017-10-27 | 2018-02-16 | 范瑶飞 | A kind of soil improvement method |
| CN108160690A (en) * | 2017-11-27 | 2018-06-15 | 浙江师范大学 | A kind of restorative procedure of heavy-metal contaminated soil |
| CN109521179A (en) * | 2018-10-06 | 2019-03-26 | 天津大学 | A method of soil function during evaluation thiocarbamide urea formaldehyde curing heavy metal contaminated soil |
| CN109678621A (en) * | 2018-12-26 | 2019-04-26 | 陕西省生物农业研究所 | A kind of method that basic soil is biological prosthetic |
| CN109880632A (en) * | 2019-03-08 | 2019-06-14 | 北京世纪阿姆斯生物工程有限公司 | It is acidified hardened soil remediation microbial inoculum and preparation method thereof and application method |
| CN111777439A (en) * | 2019-04-02 | 2020-10-16 | 河北晁群农业科技有限公司 | Soil loosening method for liquid compound microbial fertilizer |
| CN111205136A (en) * | 2020-03-09 | 2020-05-29 | 杜得信 | Soil conditioner containing sodium glutamate and amino-oligosaccharin |
| CN114621890A (en) * | 2021-03-24 | 2022-06-14 | 施可丰化工股份有限公司 | Compound microbial agent for adsorbing and removing heavy metal elements in soil and preparation method thereof |
| CN113293112B (en) * | 2021-06-21 | 2022-10-11 | 上海交通大学 | Composite microbial soil remediation agent and preparation method thereof |
| CN113402340A (en) * | 2021-07-14 | 2021-09-17 | 华联农业发展(辽宁省)有限公司 | Production method of microbial soil conditioner |
| CN113735651A (en) * | 2021-08-26 | 2021-12-03 | 黑龙江谷馨源肥业有限公司 | Soil remediation improver and preparation method thereof |
| CN115725431A (en) * | 2021-08-30 | 2023-03-03 | 武汉科诺生物科技股份有限公司 | Bacillus zoogloea and preparation method and application thereof |
| CN114085675B (en) * | 2021-11-18 | 2024-04-16 | 上海市园林工程有限公司 | Soil conditioner suitable for urban green land soil and preparation method thereof |
| CN114938814B (en) * | 2022-05-31 | 2023-08-01 | 山东京博农化科技股份有限公司 | Use of surfactant and composition thereof in promoting plant root growth |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334952C (en) * | 2005-08-31 | 2007-09-05 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
| CN101570457A (en) * | 2008-04-28 | 2009-11-04 | 安徽省文胜肥业有限责任公司 | Ecological nitrogen fertilizer and preparation method thereof |
| CN101781569A (en) * | 2009-11-27 | 2010-07-21 | 山东多利德生物科技有限公司 | Soil improvement and anti continuous cropping multifunctional biological agent |
-
2011
- 2011-09-29 CN CN201110292342.1A patent/CN102391876B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100334952C (en) * | 2005-08-31 | 2007-09-05 | 山东靠山生物科技有限公司 | Biologica land biochemical preparation and its preparing method and use |
| CN101570457A (en) * | 2008-04-28 | 2009-11-04 | 安徽省文胜肥业有限责任公司 | Ecological nitrogen fertilizer and preparation method thereof |
| CN101781569A (en) * | 2009-11-27 | 2010-07-21 | 山东多利德生物科技有限公司 | Soil improvement and anti continuous cropping multifunctional biological agent |
Non-Patent Citations (1)
| Title |
|---|
| 拟康氏木霉和枯草芽孢杆菌对黄瓜枯萎病的协同防治作用;柳春燕等;《中国生物防治》;20050808;第21卷(第3期);206-208 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111713235A (en) * | 2020-05-29 | 2020-09-29 | 金正生物化工有限公司 | High-quality high-yield fertilization method for sweet potato continuous cropping planting |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102391876A (en) | 2012-03-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102391876B (en) | Composite biological soil modifier and application thereof | |
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| CN103820348B (en) | The production method of one strain plant growth-promoting bacteria and microbial inoculum thereof and application | |
| CN107502572B (en) | Application of bacillus licheniformis in straw degradation, microbial agent containing bacillus licheniformis and application of bacillus licheniformis | |
| CN102409014B (en) | Ziziphus jujube growth-promoting rhizobacteria Bacillus subtilis and its uses | |
| CN110106126A (en) | One plant of colloid bacillus cereus and its preparing the application in saline-alkali soil conditioner | |
| CN103194410B (en) | Paenibacillus mucilaginosus and method for producing compound microorganism bacterium agent by utilizing same | |
| CN103304305A (en) | Compound-microorganism functional enteromorpha foliar fertilizer and preparation method thereof | |
| CN102071148A (en) | Trichoderma pseudokoningii (SMF2) strain and application thereof | |
| CN104130068B (en) | A kind of composite multi-functional biological foliage fertilizer | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN113321547A (en) | Rhizosphere growth promoting water-soluble microbial fertilizer and preparation method thereof | |
| CN107254420B (en) | Bacillus subtilis and application thereof | |
| CN104152149A (en) | Novel multifunctional composite biological soil improvement agent | |
| CN112592866A (en) | Carbon-based microbial agent for soil remediation and preparation method and application thereof | |
| CN103931660A (en) | Method utilizing critic acid wastewater to produce bacillus amyloliquefaciens bio-control inoculant | |
| CN109957535A (en) | Simple bacillus, microbial bacterial agent, bio-fertilizer and the application prepared using it | |
| CN117859593B (en) | Planting method for preventing and controlling bacterial wilt of continuous cropping tomatoes by using carbon-based biofertilizer | |
| CN111470899B (en) | Special organic liquid fertilizer for sugar beet and application of special organic liquid fertilizer in sugar beet planting | |
| CN102604872B (en) | Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application | |
| CN105624062B (en) | Eupatorium adenophorum organic fertilizer solid microbial inoculum, preparation method and application in organic fertilizer production | |
| CN105505819B (en) | A method of rice washing waste water production solution starch bud-fungus fertilizer is soaked using sugar industry | |
| CN104193549A (en) | Biological additive for promoting alfalfa green manure to increase crop yield | |
| CN108753657A (en) | A kind of slow-released system and application thereof | |
| CN113717001A (en) | Preparation method of disease-resistant biological bacterial fertilizer for tomatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Composite biological soil modifier and application thereof Effective date of registration: 20150717 Granted publication date: 20150304 Pledgee: Shandong agricultural financing Company limited by guarantee Pledgor: Kaoshan Biological Science and Technology Co., Ltd., Shandong Registration number: 2015990000558 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20160718 Granted publication date: 20150304 Pledgee: Shandong agricultural financing Company limited by guarantee Pledgor: Kaoshan Biological Science and Technology Co., Ltd., Shandong Registration number: 2015990000558 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Composite biological soil modifier and application thereof Effective date of registration: 20160722 Granted publication date: 20150304 Pledgee: Shandong agricultural financing Company limited by guarantee Pledgor: Kaoshan Biological Science and Technology Co., Ltd., Shandong Registration number: 2016370000049 |
|
| PLDC | Enforcement, change and cancellation of contracts on pledge of patent right or utility model | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150304 Termination date: 20200929 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |