CN106819355A - The preparation method of one seed oyster polypeptide capsule - Google Patents
The preparation method of one seed oyster polypeptide capsule Download PDFInfo
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- CN106819355A CN106819355A CN201611081142.0A CN201611081142A CN106819355A CN 106819355 A CN106819355 A CN 106819355A CN 201611081142 A CN201611081142 A CN 201611081142A CN 106819355 A CN106819355 A CN 106819355A
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- 241000237502 Ostreidae Species 0.000 title claims abstract description 166
- 235000020636 oyster Nutrition 0.000 title claims abstract description 166
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 83
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 76
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 75
- 239000002775 capsule Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 239000007788 liquid Substances 0.000 claims abstract description 34
- 239000000843 powder Substances 0.000 claims abstract description 34
- 102000004190 Enzymes Human genes 0.000 claims abstract description 32
- 108090000790 Enzymes Proteins 0.000 claims abstract description 32
- 235000013372 meat Nutrition 0.000 claims abstract description 28
- 238000001914 filtration Methods 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 12
- 238000005469 granulation Methods 0.000 claims abstract description 10
- 230000003179 granulation Effects 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 8
- 229940088598 enzyme Drugs 0.000 claims description 28
- 239000002781 deodorant agent Substances 0.000 claims description 17
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 15
- 239000002002 slurry Substances 0.000 claims description 15
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 10
- 108091005804 Peptidases Proteins 0.000 claims description 10
- 239000004365 Protease Substances 0.000 claims description 10
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 10
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 10
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 10
- 239000000194 fatty acid Substances 0.000 claims description 10
- 229930195729 fatty acid Natural products 0.000 claims description 10
- 150000004665 fatty acids Chemical class 0.000 claims description 10
- 239000011777 magnesium Substances 0.000 claims description 10
- 229910052749 magnesium Inorganic materials 0.000 claims description 10
- 235000013809 polyvinylpolypyrrolidone Nutrition 0.000 claims description 10
- 229920000523 polyvinylpolypyrrolidone Polymers 0.000 claims description 10
- 235000019419 proteases Nutrition 0.000 claims description 10
- -1 Sodium dialkyl sulfate Chemical class 0.000 claims description 9
- 239000011734 sodium Substances 0.000 claims description 9
- 229910052708 sodium Inorganic materials 0.000 claims description 9
- 239000000796 flavoring agent Substances 0.000 claims description 8
- 235000019634 flavors Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 101000693530 Staphylococcus aureus Staphylokinase Proteins 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 238000012423 maintenance Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 238000001694 spray drying Methods 0.000 claims description 5
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 4
- 102000057297 Pepsin A Human genes 0.000 claims description 4
- 108090000284 Pepsin A Proteins 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 229940111202 pepsin Drugs 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 abstract description 8
- 238000005516 engineering process Methods 0.000 abstract description 8
- 238000000605 extraction Methods 0.000 abstract description 4
- 239000007963 capsule composition Substances 0.000 abstract description 2
- 238000004108 freeze drying Methods 0.000 abstract description 2
- 230000007062 hydrolysis Effects 0.000 abstract description 2
- 238000006460 hydrolysis reaction Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000004537 pulping Methods 0.000 abstract description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 4
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 4
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 4
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- 235000004279 alanine Nutrition 0.000 description 4
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 4
- 229930182817 methionine Natural products 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000001976 enzyme digestion Methods 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 235000019658 bitter taste Nutrition 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000014103 egg white Nutrition 0.000 description 1
- 210000000969 egg white Anatomy 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000020995 raw meat Nutrition 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses the preparation method of a seed oyster polypeptide capsule.It solves the problems, such as that existing oyster polypeptide extraction efficiency is low, human body is low to oyster polypeptide absorption efficiency.It is to take oyster meat to obtain enzymolysis liquid through self-dissolving, external source enzyme resolving after raw material pulping；Enzymolysis liquid obtains oyster polypeptide powder through the enzyme that goes out, filtering, concentration, freeze-drying；Oyster polypeptide mixes with auxiliary material, granulation, is encapsulated into capsule shells, obtains oyster polypeptide capsule.It is the method being combined using self-dissolving and external source enzyme resolving, and the polypeptide hydrolysis degree of acquisition is good, and yield is high；The probability being inactivated can be reduced using capsule formulation, absorption of the human body to oyster polypeptide is improved.Preparation method rational technology of the invention, operation is feasible, easily accomplishes scale production.
Description
Technical field
The present invention relates to the preparation of peptide, the especially preparation method of a seed oyster polypeptide capsule.
Background technology
Oyster meat is fertile smooth, and delicious flavour is nutritious, have the laudatory title of " seabed milk ".Contain albumen in oyster meat
Matter is up to 45~57%, lipase 37~11%, glycogen 19~38%, additionally containing multivitamin and taurine and calcium, phosphorus,
The nutritional ingredients such as iron, zinc, close to 1 times of milk, iron content is 21 times of milk to calcium content, is beautifying and disease preventing and treating
Valuable foodstuff.Modern medicine thinks that oyster meat also has the functions such as hypotensive and nourishing yin and nourishing blood.
Due to the unique nutritional advantages of oyster, technical staff endeavours to study the healthcare applications of oyster, and at present, it is mostly with male
Oyster freeze-dried powder or oyster polypeptide extract are made various food and health food.Its treatment technology to oyster mainly has following behaviour
Make step：1st, fresh oyster is shelled and takes meat, is cleaned；2nd, water bath with thermostatic control is blended in using one or more complex enzyme to oyster meat gruel
Enzyme digestion reaction is carried out in pot；3rd, the enzyme that goes out is boiled to the oyster meat solution after enzymolysis；4th, the oyster meat solution after destroy the enzyme treatment is entered
Row hyperfiltration treatment；5th, ultrafiltration solution concentrated, freezed powder processed.In the above-mentioned extraction process to oyster meat polypeptide, exist as
Lower technical disadvantages：1st, when that can not be processed in time, active material is inactivated oyster fresh meat in causing oyster meat, reduces its battalion
Support value；2nd, common enzyme digestion reaction is most between 40~80 DEG C of temperature range, in this way, protein in oyster meat will be caused
Or the inactivation of part small-molecular peptides；3rd, the oyster polypeptide for extracting can be inactivated during preservation, reduce human small intestine to battalion
Support the absorption of material.Disadvantages mentioned above 1 and shortcoming 2 reduce effective recovery rate of oyster polypeptide, and shortcoming 3 then reduces human body
To the absorption efficiency of oyster polypeptide.
How to solve that oyster polypeptide extraction efficiency is relatively low, and how to improve the problem that human body absorbs to oyster polypeptide, into
For those skilled in the art's problem demanding prompt solution.
The content of the invention
In order to overcome the shortcomings of that oyster polypeptide extraction efficiency is low in the prior art, human body is low to oyster polypeptide absorption efficiency,
The present invention provides rational technology, operates the preparation method of a feasible seed oyster polypeptide capsule.
The technical solution adopted for the present invention to solve the technical problems is：The preparation method of one seed oyster polypeptide capsule, its
It is characterised by：It is by following process steps：
（1）The pretreatment of raw material
Fresh oyster is chosen for raw material, cleaned, peeled off, take oyster meat；By in oyster meat feeding pulper, its weight 2~5 is added
Water again, is made oyster slurries；
（2）Self-dissolving
By step（1）In obtain oyster slurries feeding aseptic tank in, use sodium hydroxide solution adjust pH value be 6~7.5, control
0~4 DEG C of temperature processed, 24~48h of self-dissolving；
（3）Enzymolysis
By step（2）In oyster slurries after the self-dissolving that obtains pH value to 4~5 is adjusted using citric acid solution, add oyster meat
The compound protease of weight 0.2~0.5%, controls 40~45 DEG C of temperature, digests 1~2h, obtains oyster enzymolysis liquid；
（4）Go out enzyme
By step（3）In the oyster enzymolysis liquid that obtains be heated to 90~100 DEG C, maintenance is gone out 20~30min of enzyme, the oyster of the enzyme that must go out
Enzymolysis liquid；
（5）Deodorant
By step（4）In oyster enzymolysis liquid after the enzyme that goes out that obtains add the glucose of its weight 0.2~0.4%, be heated to 90~
100 DEG C, 20~30min of defishying obtains the oyster enzymolysis liquid of deodorant；
（6）Filtering
By step（5）In after the oyster enzymolysis liquid coarse filtration of deodorant that obtains, through micro-pore-film filtration, remove tiny suspended particulate, obtain
Oyster clarified solution；
（7）Dry
By step（6）In obtain oyster clarified solution feeding vacuum concentration equipment in, control 45~65 DEG C of temperature, be concentrated into solid
Thing content is 12~15%, spray drying, makes its moisture less than 4%, obtains oyster polypeptide powder；
（8）Granulation
By step（7）In the oyster polypeptide powder that obtains be well mixed with auxiliary material, pelletize, be encapsulated into capsule shells, obtain oyster many
Peptide capsule.
Described compound protease be in pepsin, hay bacillus, neutral proteinase, flavor protease it is a kind of with
On.
Described auxiliary material includes sodium dialkyl sulfate, fatty acid magnesium, talcum powder and PVPP, wherein, described dioxane
The consumption of base sodium sulphate is the 5~6% of oyster polypeptide powder weight, and the consumption of described fatty acid magnesium is oyster polypeptide powder weight
5~6%, the consumption of described talcum powder is the 10~12% of oyster polypeptide powder weight, the consumption of described PVPP
It is the 10~12% of oyster polypeptide powder weight.
The present invention with oyster meat as raw material pulping after through self-dissolving, external source enzyme resolving acquisition enzymolysis liquid；Enzymolysis liquid is through the enzyme that goes out, mistake
Filter, concentration, freeze-drying obtains oyster polypeptide powder；Oyster polypeptide mixes with auxiliary material, granulation, is encapsulated into capsule shells, obtains male
Oyster polypeptide capsule.The method that the present invention is combined using self-dissolving and external source enzyme resolving, and the complex enzyme for being used is by stomach egg
White enzyme, hay bacillus, neutral proteinase, flavor protease are combined, and the polypeptide hydrolysis degree of acquisition is good, and yield is high, and wind
Taste protease carries out local flavor optimization to hydrolysate, removes bitter taste, improves mouthfeel.After testing, oyster of the molecular mass less than 1Kda
Peptide accounts for more than 80%, and gained polypeptide, histidine, alanine, methionine leucine content are high, with stronger antioxidation.
The present invention uses capsule formulation, makes oyster polypeptide that the probability being inactivated is reduced during preservation, improves human small intestine to nutrition
The absorption of material, preferably solves the problems, such as that oyster polypeptide absorbs.Preparation method rational technology of the invention, operation is feasible, easily
Accomplish scale production.
Specific embodiment
With reference to embodiment to being originally described further.
Embodiment 1
The preparation method of one seed oyster polypeptide capsule, it is characterised in that：It is by following process steps：
（1）The pretreatment of raw material
Fresh oyster is chosen for raw material, cleaned, peeled off, take oyster meat；By in oyster meat feeding pulper, 3 times of its weight is added
Water, be made oyster slurries；
（2）Self-dissolving
By step（1）In obtain oyster slurries feeding aseptic tank in, use sodium hydroxide solution adjust pH value be 7.0, control
2 DEG C of temperature, self-dissolving 36h；
（3）Enzymolysis
By step（2）In oyster slurries after the self-dissolving that obtains pH value to 4.5 is adjusted using citric acid solution, add oyster meat weight
0.2% pepsin, 0.1% neutral proteinase and 0.05% flavor protease is measured, 43 DEG C of temperature is controlled, 1.5h is digested, obtained
Oyster enzymolysis liquid；
（4）Go out enzyme
By step（3）In the oyster enzymolysis liquid that obtains be heated to 95 DEG C, maintenance is gone out enzyme 30min, the oyster enzymolysis liquid of the enzyme that must go out；
（5）Deodorant
By step（4）In oyster enzymolysis liquid after the enzyme that goes out that obtains add the glucose of its weight 0.3%, be heated to 95 DEG C, deodorant
Treatment 25min, obtains the oyster enzymolysis liquid of deodorant；
（6）Filtering
By step（5）In after the oyster enzymolysis liquid coarse filtration of deodorant that obtains, through micro-pore-film filtration, remove tiny suspended particulate, obtain
Oyster clarified solution；
（7）Dry
By step（6）In obtain oyster clarified solution feeding vacuum concentration equipment in, control 55 DEG C of temperature, be concentrated into solid content and contain
It is 14% to measure, spray drying, makes its moisture less than 3%, obtains oyster polypeptide powder；
（8）Granulation
By step（7）In the oyster polypeptide powder that obtains add sodium dialkyl sulfate, fatty acid magnesium, talcum powder and PVPP,
It is well mixed；Wherein, the consumption of sodium dialkyl sulfate is the 5.5% of oyster polypeptide powder weight, and the consumption of fatty acid magnesium is that oyster is more
The 5.5% of peptide powder weight, the consumption of talcum powder is the 10.5% of oyster polypeptide powder weight, and the consumption of PVPP is oyster
The 11% of polypeptide powder weight, then routinely technique granulation, are encapsulated into capsule shells by 5g/, obtain oyster polypeptide capsule.
The preparation method of the oyster polypeptide capsule that the present embodiment is provided, its rational technology, operation is reliable.Using the method
The oyster polypeptide capsule of preparation its polypeptide, histidine, alanine, methionine leucine content are high, with stronger anti-oxidant
Effect, it is easy to absorption of human body.
Embodiment 2
The preparation method of one seed oyster polypeptide capsule, it is characterised in that：It is by following process steps：
（1）The pretreatment of raw material
Fresh oyster is chosen for raw material, cleaned, peeled off, take oyster meat；By in oyster meat feeding pulper, 2 times of its weight is added
Water, be made oyster slurries；
（2）Self-dissolving
By step（1）In obtain oyster slurries feeding aseptic tank in, use sodium hydroxide solution adjust pH value be 7.5, control
0 DEG C of temperature, self-dissolving 48h；
（3）Enzymolysis
By step（2）In oyster slurries after the self-dissolving that obtains pH value to 4.5 is adjusted using citric acid solution, add oyster meat weight
0.3% hay bacillus, 0.1% neutral proteinase and 0.1% flavor protease is measured, 40 DEG C of temperature is controlled, 1h is digested, obtains male
Oyster enzymolysis liquid；
（4）Go out enzyme
By step（3）In the oyster enzymolysis liquid that obtains be heated to 95 DEG C, maintenance is gone out enzyme 25min, the oyster enzymolysis liquid of the enzyme that must go out；
（5）Deodorant
By step（4）In oyster enzymolysis liquid after the enzyme that goes out that obtains add the glucose of its weight 0.2%, be heated to 90 DEG C, deodorant
Treatment 30min, obtains the oyster enzymolysis liquid of deodorant；
（6）Filtering
By step（5）In after the oyster enzymolysis liquid coarse filtration of deodorant that obtains, through micro-pore-film filtration, remove tiny suspended particulate, obtain
Oyster clarified solution；
（7）Dry
By step（6）In obtain oyster clarified solution feeding vacuum concentration equipment in, control temperature 45 C, be concentrated into solid content and contain
It is 12% to measure, spray drying, makes its moisture less than 4%, obtains oyster polypeptide powder；
（8）Granulation
By step（7）In the oyster polypeptide powder that obtains add sodium dialkyl sulfate, fatty acid magnesium, talcum powder and PVPP,
It is well mixed；Wherein, the consumption of sodium dialkyl sulfate is the 5% of oyster polypeptide powder weight, and the consumption of fatty acid magnesium is oyster polypeptide
The 6% of powder weight, the consumption of talcum powder is the 12% of oyster polypeptide powder weight, and the consumption of PVPP is oyster polypeptide powder
The 10% of last weight, then routinely technique granulation, are encapsulated into capsule shells by 6g/, obtain oyster polypeptide capsule.
The preparation method of the oyster polypeptide capsule that the present embodiment is provided, its rational technology, operation is reliable.Using the method
The oyster polypeptide capsule of preparation its polypeptide, histidine, alanine, methionine leucine content are high, and molecular mass is less than 1Kda
Oyster peptide account for more than 80%, with stronger antioxidation, human absorptivity is high.
Embodiment 3
The preparation method of one seed oyster polypeptide capsule, it is characterised in that：It is by following process steps：
（1）The pretreatment of raw material
Fresh oyster is chosen for raw material, cleaned, peeled off, take oyster meat；By in oyster meat feeding pulper, 5 times of its weight is added
Water, be made oyster slurries；
（2）Self-dissolving
By step（1）In obtain oyster slurries feeding aseptic tank in, use sodium hydroxide solution adjust pH value be 6.0, control
4 DEG C of temperature, self-dissolving 24h；
（3）Enzymolysis
By step（2）In oyster slurries after the self-dissolving that obtains pH value to 5 is adjusted using citric acid solution, add oyster meat weight
0.1% pepsin, 0.1% flavor protease, control temperature 45 C, digest 2h, obtain oyster enzymolysis liquid；
（4）Go out enzyme
By step（3）In the oyster enzymolysis liquid that obtains be heated to 90 DEG C, maintenance is gone out enzyme 30min, the oyster enzymolysis liquid of the enzyme that must go out；
（5）Deodorant
By step（4）In oyster enzymolysis liquid after the enzyme that goes out that obtains add the glucose of its weight 0.4%, be heated to 100 DEG C, take off
Raw meat processes 20min, obtains the oyster enzymolysis liquid of deodorant；
（6）Filtering
By step（5）In after the oyster enzymolysis liquid coarse filtration of deodorant that obtains, through micro-pore-film filtration, remove tiny suspended particulate, obtain
Oyster clarified solution；
（7）Dry
By step（6）In obtain oyster clarified solution feeding vacuum concentration equipment in, control 65 DEG C of temperature, be concentrated into solid content and contain
It is 15% to measure, spray drying, makes its moisture less than 3.5%, obtains oyster polypeptide powder；
（8）Granulation
By step（7）In the oyster polypeptide powder that obtains add sodium dialkyl sulfate, fatty acid magnesium, talcum powder and PVPP,
It is well mixed；Wherein, the consumption of sodium dialkyl sulfate is the 6% of oyster polypeptide powder weight, and the consumption of fatty acid magnesium is oyster polypeptide
The 5% of powder weight, the consumption of talcum powder is the 10% of oyster polypeptide powder weight, and the consumption of PVPP is oyster polypeptide powder
The 12% of last weight, then routinely technique granulation, are encapsulated into capsule shells by 5g/, obtain oyster polypeptide capsule.
The preparation method of the oyster polypeptide capsule that the present embodiment is provided, its rational technology, operation is reliable.Using the method
The oyster polypeptide capsule of preparation, its polypeptide, histidine, alanine, methionine leucine content are high, with stronger anti-oxidant
Effect is same, it is easy to absorption of human body.
Claims (3)
1. the preparation method of a seed oyster polypeptide capsule, it is characterised in that：It is by following process steps：
（1）The pretreatment of raw material
Fresh oyster is chosen for raw material, cleaned, peeled off, take oyster meat；By in oyster meat feeding pulper, its weight 2~5 is added
Water again, is made oyster slurries；
（2）Self-dissolving
By step（1）In obtain oyster slurries feeding aseptic tank in, use sodium hydroxide solution adjust pH value be 6~7.5, control
0~4 DEG C of temperature processed, 24~48h of self-dissolving；
（3）Enzymolysis
By step（2）In oyster slurries after the self-dissolving that obtains pH value to 4~5 is adjusted using citric acid solution, add oyster meat
The compound protease of weight 0.2~0.5%, controls 40~45 DEG C of temperature, digests 1~2h, obtains oyster enzymolysis liquid；
（4）Go out enzyme
By step（3）In the oyster enzymolysis liquid that obtains be heated to 90~100 DEG C, maintenance is gone out 20~30min of enzyme, the oyster of the enzyme that must go out
Enzymolysis liquid；
（5）Deodorant
By step（4）In oyster enzymolysis liquid after the enzyme that goes out that obtains add the glucose of its weight 0.2~0.4%, be heated to 90~
100 DEG C, 20~30min of defishying obtains the oyster enzymolysis liquid of deodorant；
（6）Filtering
By step（5）In after the oyster enzymolysis liquid coarse filtration of deodorant that obtains, through micro-pore-film filtration, remove tiny suspended particulate, obtain
Oyster clarified solution；
（7）Dry
By step（6）In obtain oyster clarified solution feeding vacuum concentration equipment in, control 45~65 DEG C of temperature, be concentrated into solid
Thing content is 12~15%, spray drying, makes its moisture less than 4%, obtains oyster polypeptide powder；
（8）Granulation
By step（7）In the oyster polypeptide powder that obtains be well mixed with auxiliary material, pelletize, be encapsulated into capsule shells, obtain oyster many
Peptide capsule.
2. the preparation method of seed oyster polypeptide capsule according to claim 1, it is characterised in that：Described compound protein
Enzyme is the one or more than one in pepsin, hay bacillus, neutral proteinase, flavor protease.
3. the preparation method of seed oyster polypeptide capsule according to claim 1, it is characterised in that：Described auxiliary material includes
Sodium dialkyl sulfate, fatty acid magnesium, talcum powder and PVPP, wherein, the consumption of described sodium dialkyl sulfate is that oyster is more
The 5~6% of peptide powder weight, the consumption of described fatty acid magnesium is the 5~6% of oyster polypeptide powder weight, described talcum powder
Consumption is the 10~12% of oyster polypeptide powder weight, and the consumption of described PVPP is the 10 of oyster polypeptide powder weight
~12%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832625A (en) * | 2018-08-20 | 2019-06-04 | 北京姿美堂生物技术有限公司 | A kind of composition and preparation method thereof for alleviating physical fatigue strengthen immunity |
| CN114794339A (en) * | 2022-05-07 | 2022-07-29 | 广东还珠海洋生物科技有限公司 | Fresh oyster beverage |
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| CN1478420A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Oyster capsule food and its preparation method |
| CN1803836A (en) * | 2006-01-13 | 2006-07-19 | 国家海洋局第一海洋研究所 | Jellyfish collagen and method for preparing the same |
| CN101273785A (en) * | 2007-03-28 | 2008-10-01 | 天津天狮生物发展有限公司 | Xinbujing capsule and producing process thereof |
| CN102551043A (en) * | 2011-10-29 | 2012-07-11 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide nutrition supplement food for children and preparation method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1478420A (en) * | 2002-08-30 | 2004-03-03 | 大连轻工业学院 | Oyster capsule food and its preparation method |
| CN1803836A (en) * | 2006-01-13 | 2006-07-19 | 国家海洋局第一海洋研究所 | Jellyfish collagen and method for preparing the same |
| CN101273785A (en) * | 2007-03-28 | 2008-10-01 | 天津天狮生物发展有限公司 | Xinbujing capsule and producing process thereof |
| CN102551043A (en) * | 2011-10-29 | 2012-07-11 | 山东好当家海洋发展股份有限公司 | Oyster polypeptide nutrition supplement food for children and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109832625A (en) * | 2018-08-20 | 2019-06-04 | 北京姿美堂生物技术有限公司 | A kind of composition and preparation method thereof for alleviating physical fatigue strengthen immunity |
| CN115581300A (en) * | 2018-08-20 | 2023-01-10 | 北京姿美堂生物技术有限公司 | Composition for relieving fatigue and enhancing immunity and preparation method thereof |
| CN114794339A (en) * | 2022-05-07 | 2022-07-29 | 广东还珠海洋生物科技有限公司 | Fresh oyster beverage |
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