CN106666739A - Preparation method of pomegranate polyphenols - Google Patents
Preparation method of pomegranate polyphenols Download PDFInfo
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- CN106666739A CN106666739A CN201611178935.4A CN201611178935A CN106666739A CN 106666739 A CN106666739 A CN 106666739A CN 201611178935 A CN201611178935 A CN 201611178935A CN 106666739 A CN106666739 A CN 106666739A
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- punica granatum
- polyphenol
- rotary evaporation
- lyophilization
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- 235000014360 Punica granatum Nutrition 0.000 title claims abstract description 48
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 41
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241000219991 Lythraceae Species 0.000 title claims abstract description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000002390 rotary evaporation Methods 0.000 claims abstract description 17
- 238000005374 membrane filtration Methods 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 11
- 244000294611 Punica granatum Species 0.000 claims description 37
- 238000004108 freeze drying Methods 0.000 claims description 19
- 238000000605 extraction Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 11
- 241001312402 Helvella corium Species 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- 230000014759 maintenance of location Effects 0.000 claims 1
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract description 2
- 230000000975 bioactive effect Effects 0.000 abstract 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- REFJWTPEDVJJIY-UHFFFAOYSA-N Quercetin Chemical compound C=1C(O)=CC(O)=C(C(C=2O)=O)C=1OC=2C1=CC=C(O)C(O)=C1 REFJWTPEDVJJIY-UHFFFAOYSA-N 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- -1 polyphenol hydroxyl compound Chemical class 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- PFTAWBLQPZVEMU-DZGCQCFKSA-N (+)-catechin Chemical compound C1([C@H]2OC3=CC(O)=CC(O)=C3C[C@@H]2O)=CC=C(O)C(O)=C1 PFTAWBLQPZVEMU-DZGCQCFKSA-N 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-M (E)-Ferulic acid Natural products COC1=CC(\C=C\C([O-])=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-M 0.000 description 1
- CWVRJTMFETXNAD-FWCWNIRPSA-N 3-O-Caffeoylquinic acid Natural products O[C@H]1[C@@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-FWCWNIRPSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- PZIRUHCJZBGLDY-UHFFFAOYSA-N Caffeoylquinic acid Natural products CC(CCC(=O)C(C)C1C(=O)CC2C3CC(O)C4CC(O)CCC4(C)C3CCC12C)C(=O)O PZIRUHCJZBGLDY-UHFFFAOYSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- AFSDNFLWKVMVRB-UHFFFAOYSA-N Ellagic acid Chemical compound OC1=C(O)C(OC2=O)=C3C4=C2C=C(O)C(O)=C4OC(=O)C3=C1 AFSDNFLWKVMVRB-UHFFFAOYSA-N 0.000 description 1
- ATJXMQHAMYVHRX-CPCISQLKSA-N Ellagic acid Natural products OC1=C(O)[C@H]2OC(=O)c3cc(O)c(O)c4OC(=O)C(=C1)[C@H]2c34 ATJXMQHAMYVHRX-CPCISQLKSA-N 0.000 description 1
- 229920002079 Ellagic acid Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- CWVRJTMFETXNAD-KLZCAUPSSA-N Neochlorogenin-saeure Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O CWVRJTMFETXNAD-KLZCAUPSSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- ZVOLCUVKHLEPEV-UHFFFAOYSA-N Quercetagetin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=C(O)C(O)=C(O)C=C2O1 ZVOLCUVKHLEPEV-UHFFFAOYSA-N 0.000 description 1
- HWTZYBCRDDUBJY-UHFFFAOYSA-N Rhynchosin Natural products C1=C(O)C(O)=CC=C1C1=C(O)C(=O)C2=CC(O)=C(O)C=C2O1 HWTZYBCRDDUBJY-UHFFFAOYSA-N 0.000 description 1
- 241001593750 Turcica Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229930014669 anthocyanidin Natural products 0.000 description 1
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 1
- 235000008758 anthocyanidins Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 238000006701 autoxidation reaction Methods 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- ADRVNXBAWSRFAJ-UHFFFAOYSA-N catechin Natural products OC1Cc2cc(O)cc(O)c2OC1c3ccc(O)c(O)c3 ADRVNXBAWSRFAJ-UHFFFAOYSA-N 0.000 description 1
- 235000005487 catechin Nutrition 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 229940074393 chlorogenic acid Drugs 0.000 description 1
- 235000001368 chlorogenic acid Nutrition 0.000 description 1
- CWVRJTMFETXNAD-JUHZACGLSA-N chlorogenic acid Chemical compound O[C@@H]1[C@H](O)C[C@@](O)(C(O)=O)C[C@H]1OC(=O)\C=C\C1=CC=C(O)C(O)=C1 CWVRJTMFETXNAD-JUHZACGLSA-N 0.000 description 1
- FFQSDFBBSXGVKF-KHSQJDLVSA-N chlorogenic acid Natural products O[C@@H]1C[C@](O)(C[C@@H](CC(=O)C=Cc2ccc(O)c(O)c2)[C@@H]1O)C(=O)O FFQSDFBBSXGVKF-KHSQJDLVSA-N 0.000 description 1
- 229950001002 cianidanol Drugs 0.000 description 1
- BMRSEYFENKXDIS-KLZCAUPSSA-N cis-3-O-p-coumaroylquinic acid Natural products O[C@H]1C[C@@](O)(C[C@@H](OC(=O)C=Cc2ccc(O)cc2)[C@@H]1O)C(=O)O BMRSEYFENKXDIS-KLZCAUPSSA-N 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 229960002852 ellagic acid Drugs 0.000 description 1
- 235000004132 ellagic acid Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000001785 ferulic acid Nutrition 0.000 description 1
- KSEBMYQBYZTDHS-HWKANZROSA-N ferulic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC=C1O KSEBMYQBYZTDHS-HWKANZROSA-N 0.000 description 1
- 229940114124 ferulic acid Drugs 0.000 description 1
- KSEBMYQBYZTDHS-UHFFFAOYSA-N ferulic acid Natural products COC1=CC(C=CC(O)=O)=CC=C1O KSEBMYQBYZTDHS-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000004515 gallic acid Nutrition 0.000 description 1
- 229940074391 gallic acid Drugs 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000014058 juice drink Nutrition 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- FAARLWTXUUQFSN-UHFFFAOYSA-N methylellagic acid Natural products O1C(=O)C2=CC(O)=C(O)C3=C2C2=C1C(OC)=C(O)C=C2C(=O)O3 FAARLWTXUUQFSN-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 235000021426 pomegranate vinegar Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229960001285 quercetin Drugs 0.000 description 1
- 235000005875 quercetin Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- QURCVMIEKCOAJU-UHFFFAOYSA-N trans-isoferulic acid Natural products COC1=CC=C(C=CC(O)=O)C=C1O QURCVMIEKCOAJU-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a preparation method of pomegranate polyphenols. The preparation method comprises the following steps: pomegranate peels are crushed, the crushed pomegranate peels are extracted by alcohol, an alcohol extract is subjected to a membrane filtration, the filtered extract is subjected to a rotary evaporation and concentration, and finally concentrate is freeze-dried. Beneficial effects are as follows: the preparation method effectively maintains biological activity of the polyphenols and improves bioavailability in a normal environment. The preparation method solves the problem that the bioactive components are easy to oxidize and not stable in the application processes to a certain extent, greatly extends a preservation period of the pomegranate polyphenols, and improves possibility of the applications of the various product forms of the pomegranate polyphenols.
Description
Technical field
The present invention relates to the preparing technical field of the extraction of effective components in plants and health product, more particularly to a kind of Punica granatum L.
The preparation method of polyphenol.
Background technology
Punica granatum L. (Punicagranatum) polyphenol is the general name that a class has polyphenol hydroxyl compound, including elladitannin,
The multiple compounds such as Galla Turcica (Galla Helepensis) tannin, ellagic acid, gallic acid, catechin, anthocyanidin, chlorogenic acid, ferulic acid and quercetin, mainly
In being present in Pericarpium Granati, content is about the 10%~20% of its dry mass, with antioxidation, defying age, anti-cancer and cancer-preventing, antibacterial,
Various physiology and the pharmacologically actives such as moistening skin beautifying, blood pressure lowering and prevention cardiovascular and cerebrovascular disease.The non-oxidizability of Punica granatum L. polyphenol and anti-
Bacterium activity is higher, and in fields such as food, medicine and household chemicalss huge using value is shown.At present to Punica granatum L. industry
The application of change, more for preparing the tradition juice drinks such as Sucus Granati, Pomegranate vinegar, be also by the active component in extraction Pericarpium Granati
The important subject of Punica granatum L. industry higher value application.It is fewer to the report of many phenol extractions of Pericarpium Granati both at home and abroad at present, it is existing
Some methods mostly are decocting method, the features such as the method has easy to operate and low cost, but easily cause in Pericarpium Granati Polyphenols and
The destruction of other antioxidant contents, and impurity content is higher in extracting solution, is unfavorable for the isolation and purification of polyphenol.Therefore, very
The method for being necessary to find the autoxidation for avoiding Punica granatum L. polyphenol in extraction process, with it its active stability is ensured.
Phenolic hydroxyl group activity contained in polyphenol molecule is very strong, is highly prone to the shadow of the environmental conditions such as high temperature, humidity, illumination
Ring, the reactions such as polymerization, oxidation, condensation easily occur and loses the biological activitys such as antioxidation.Polyphenol substance is mostly powdered, granularity
It is very thin, the moisture absorption is more easy in natural environment and is lumpd, thus the polyphenol substance isolated, in storage, transport and application process easily
The Oxidation of generation can substantially reduce its using effect.
The content of the invention
The present invention proposes a kind of preparation method of Punica granatum L. polyphenol.
A kind of preparation method of Punica granatum L. polyphenol, containing following steps:
Alcohol extraction after Pericarpium Granati is crushed, alcohol extract concentrated by rotary evaporation Jing after membrane filtration, finally by concentrated solution lyophilization.
Further, the film that membrane filtration is used is film that molecular cut off is 80,000-9 ten thousand Da.
Further, concrete step is as follows:
(1) crush after is dried fresh pomegranate, cross 40 mesh sieves, Punica granatum L. corium farinosum is added afterwards the second of 20-30 times of weight
3-5 hours are extracted in alcoholic solution；
(2) extract 3 times as stated above, united extraction liquid；
(3) by membrance separation that said extracted liquid Jing molecular cut offs are 80,000-9 ten thousand Da；
(4) it is that Punica granatum L. polyphenol extracting solution between 80,000-9 ten thousand Da is placed on vacuum rotating by the molecular weight that said method is obtained
In evaporimeter, rotary evaporation, concentration volume for original volume 1/4 to 1/3 after, lyophilization obtain Punica granatum L. polyphenol dry powder extraction
Thing.
Further：The volume fraction of the ethanol solution in step (1) is 50%-75%；Extracting temperature is 50-85 DEG C.
Further：The temperature of the rotary evaporation in step (4) is 30-40 DEG C, and vacuum is -0.05MPa-0.1MPa；It is cold
Lyophilizing is dry for vacuum lyophilization, temperature：- 20 DEG C, 60h.
Beneficial effects of the present invention：
By the extraction process of Punica granatum L. polyphenol in the middle of of the invention, make largely to remain the activity in the middle of Punica granatum L. polyphenol
Composition, is that the extracted amount of Punica granatum L. polyphenol is maximum.In addition, the Punica granatum L. polyphenol after extracting can be applied to the research and development of other Related products
Raw material.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1：
A kind of preparation method of Punica granatum L. polyphenol, containing following steps:
Alcohol extraction after Pericarpium Granati is crushed, alcohol extract concentrated by rotary evaporation Jing after membrane filtration, finally by concentrated solution lyophilization.
The film that membrane filtration is used is film that molecular cut off is 80,000-9 ten thousand Da.
Further, concrete step is as follows:
(1) crush after is dried fresh pomegranate, cross 40 mesh sieves, afterwards by the 50% of Punica granatum L. corium farinosum 30 times of weight of addition
Extract 3 hours in ethanol solution；Extracting temperature is 50 DEG C.
(2) extract 3 times as stated above, united extraction liquid；
(3) by membrance separation that said extracted liquid Jing molecular cut offs are 80,000-9 ten thousand Da；
(4) it is that Punica granatum L. polyphenol extracting solution between 80,000-9 ten thousand Da is placed on vacuum rotating by the molecular weight that said method is obtained
In evaporimeter, rotary evaporation, the temperature of rotary evaporation is 30 DEG C, and vacuum is 0.1MPa；Lyophilization is vacuum lyophilization,
Temperature：- 20 DEG C, 60h.Concentration volume for original volume 1/4 after, lyophilization obtains Punica granatum L. polyphenol powder extract.
Embodiment 2：
A kind of preparation method of Punica granatum L. polyphenol, containing following steps:
Alcohol extraction after Pericarpium Granati is crushed, alcohol extract concentrated by rotary evaporation Jing after membrane filtration, finally by concentrated solution lyophilization.
The film that membrane filtration is used is film that molecular cut off is 80,000-9 ten thousand Da.
Further, concrete step is as follows:
(1) crush after is dried fresh pomegranate, cross 40 mesh sieves, afterwards by the 70% of Punica granatum L. corium farinosum 25 times of weight of addition
Extract 3 hours in ethanol solution；Extracting temperature is 50 DEG C.
(2) extract 3 times as stated above, united extraction liquid；
(3) by membrance separation that said extracted liquid Jing molecular cut offs are 80,000-9 ten thousand Da；
(4) it is that Punica granatum L. polyphenol extracting solution between 80,000-9 ten thousand Da is placed on vacuum rotating by the molecular weight that said method is obtained
In evaporimeter, rotary evaporation, the temperature of rotary evaporation is 40 DEG C, and vacuum is 0.1MPa；Lyophilization is vacuum lyophilization,
Temperature：- 20 DEG C, 60h.Concentration volume for original volume 1/3 after, lyophilization obtains Punica granatum L. polyphenol powder extract.
Embodiment 3：
A kind of preparation method of Punica granatum L. polyphenol, containing following steps:
Alcohol extraction after Pericarpium Granati is crushed, alcohol extract concentrated by rotary evaporation Jing after membrane filtration, finally by concentrated solution lyophilization.
The film that membrane filtration is used is film that molecular cut off is 80,000-9 ten thousand Da.
Further, concrete step is as follows:
(1) crush after is dried fresh pomegranate, cross 40 mesh sieves, afterwards by the 60% of Punica granatum L. corium farinosum 20 times of weight of addition
Extract 3 hours in ethanol solution；Extracting temperature is 50 DEG C.
(2) extract 3 times as stated above, united extraction liquid；
(3) by membrance separation that said extracted liquid Jing molecular cut offs are 80,000-9 ten thousand Da；
(4) it is that Punica granatum L. polyphenol extracting solution between 80,000-9 ten thousand Da is placed on vacuum rotating by the molecular weight that said method is obtained
In evaporimeter, rotary evaporation, the temperature of rotary evaporation is 30 DEG C, and vacuum is 0.1MPa；Lyophilization is vacuum lyophilization,
Temperature：- 20 DEG C, 60h.Concentration volume for original volume 1/3 after, lyophilization obtains Punica granatum L. polyphenol powder extract.
Moreover, it will be appreciated that although this specification is been described by according to embodiment, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of description is only that for clarity those skilled in the art should
Using description as an entirety, the technical scheme in each embodiment can also Jing it is appropriately combined, form those skilled in the art
Understandable other embodiment.
Claims (5)
1. a kind of preparation method of Punica granatum L. polyphenol, it is characterised in that comprise the steps of:
Alcohol extraction after Pericarpium Granati is crushed, alcohol extract concentrated by rotary evaporation Jing after membrane filtration, finally by concentrated solution lyophilization.
2. the preparation method of Punica granatum L. polyphenol according to claim 1, it is characterised in that the film that membrane filtration is used is retention point
Son amount is the film of 80,000-9 ten thousand Da.
3. the preparation method of Punica granatum L. polyphenol according to claim 1, it is characterised in that concrete step is as follows:
(1) crush after is dried fresh pomegranate, cross 40 mesh sieves, add the ethanol of 20-30 times of weight molten Punica granatum L. corium farinosum afterwards
3-5- hours are extracted in liquid；
(2) extract 3 times as stated above, united extraction liquid；
(3) by membrance separation that said extracted liquid Jing molecular cut offs are 80,000-9 ten thousand Da；
(4) it is that Punica granatum L. polyphenol extracting solution between 80,000-9 ten thousand Da is placed on rotary evaporation in vacuo by the molecular weight that said method is obtained
In instrument, rotary evaporation, concentration volume for original volume 1/4 to 1/3 after, lyophilization obtains Punica granatum L. polyphenol powder extract.
4. the preparation method of Punica granatum L. polyphenol according to claim 3, it is characterised in that：Ethanol solution in step (1)
Volume fraction is 50%-75%；Extracting temperature is 50-85 DEG C.
5. the preparation method of Punica granatum L. polyphenol according to claim 4, it is characterised in that：Rotary evaporation in step (4)
Temperature is 30-40 DEG C, and vacuum is -0.05MPa-0.1MPa；Lyophilization is vacuum lyophilization, temperature：- 20 DEG C, 60h.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611178935.4A CN106666739A (en) | 2016-12-19 | 2016-12-19 | Preparation method of pomegranate polyphenols |
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| CN201611178935.4A CN106666739A (en) | 2016-12-19 | 2016-12-19 | Preparation method of pomegranate polyphenols |
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| CN201611178935.4A Pending CN106666739A (en) | 2016-12-19 | 2016-12-19 | Preparation method of pomegranate polyphenols |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549317A (en) * | 2017-10-11 | 2018-01-09 | 西安宏兴乳业有限公司 | A kind of antioxidant and anti-aging fruity goat milk powder and preparation method thereof |
| CN110051709A (en) * | 2019-06-18 | 2019-07-26 | 江苏经贸职业技术学院 | A kind of blackcurrant polyphenol extract and preparation method thereof |
| CN112168837A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Prescription of oral solid preparation of mitochondrion element and its preparing process |
| JP2022550156A (en) * | 2019-10-01 | 2022-11-30 | ロレアル | Punica granatum extract and its cosmetic use |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107549317A (en) * | 2017-10-11 | 2018-01-09 | 西安宏兴乳业有限公司 | A kind of antioxidant and anti-aging fruity goat milk powder and preparation method thereof |
| CN110051709A (en) * | 2019-06-18 | 2019-07-26 | 江苏经贸职业技术学院 | A kind of blackcurrant polyphenol extract and preparation method thereof |
| CN110051709B (en) * | 2019-06-18 | 2021-09-14 | 江苏经贸职业技术学院 | Blackcurrant polyphenol extract and preparation method thereof |
| JP2022550156A (en) * | 2019-10-01 | 2022-11-30 | ロレアル | Punica granatum extract and its cosmetic use |
| JP7566894B2 (en) | 2019-10-01 | 2024-10-15 | ロレアル | Punica granatum extract and its cosmetic use |
| CN112168837A (en) * | 2020-06-29 | 2021-01-05 | 武汉林宝莱生物科技有限公司 | Prescription of oral solid preparation of mitochondrion element and its preparing process |
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