CN106086003A - A kind of nucleic acid purification system - Google Patents
A kind of nucleic acid purification system Download PDFInfo
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- CN106086003A CN106086003A CN201610442151.1A CN201610442151A CN106086003A CN 106086003 A CN106086003 A CN 106086003A CN 201610442151 A CN201610442151 A CN 201610442151A CN 106086003 A CN106086003 A CN 106086003A
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- 238000001821 nucleic acid purification Methods 0.000 title claims abstract description 108
- 239000007788 liquid Substances 0.000 claims abstract description 117
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 77
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 77
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 77
- 238000000746 purification Methods 0.000 claims abstract description 44
- 238000000605 extraction Methods 0.000 claims abstract description 31
- 239000012528 membrane Substances 0.000 claims description 42
- 230000037361 pathway Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 3
- 239000007924 injection Substances 0.000 claims description 3
- 230000035515 penetration Effects 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 14
- 239000000463 material Substances 0.000 abstract description 11
- 230000008569 process Effects 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 22
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 238000000197 pyrolysis Methods 0.000 description 4
- 230000003834 intracellular effect Effects 0.000 description 3
- 239000002253 acid Substances 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000012487 rinsing solution Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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Abstract
The invention discloses a kind of nucleic acid purification system, belong to experiment consumptive material field.This system includes: nucleic acid purification post, described nucleic acid purification system includes: nucleic acid purification post, the top of described nucleic acid purification post is provided with purification column inlet, the bottom of described nucleic acid purification post is provided with purification column liquid outlet, it is disposed with nucleic acid extraction film in described nucleic acid purification post, described nucleic acid extraction film covers on described purification column liquid outlet, described nucleic acid purification system includes: liquid selective passes through device, this liquid selective is the most waterproof and pass through the most for liquid through device, described liquid selective is arranged in described nucleic acid purification post through device, described liquid selective passes through device between described purification column inlet and described nucleic acid extraction film.This nucleic acid purification system is avoided using machine pipettor, makes the pipetting processes of automatic nucleic acid purification instrument simplify by liquid selective through device.
Description
Technical field
The present invention relates to test consumptive material field, particularly to a kind of nucleic acid purification system.
Background technology
Extract from vegeto-animal cell or the method for purification of nucleic acid has multiple, but most common method is pillar at present
Method, column method accounts for the 95% of the market share of all method for extracting nucleic acid.
The principle of existing column method purification of nucleic acid is nucleic acid filter membrane or adsorbed film to be installed to inside a pillar, from
And form nucleic acid Filter column or adsorption column, and when nucleic acid solution to be purified is by Filter column or adsorption column, nucleic acid to be purified
Impurity in solution is filtered by Filter column or nucleic acid is adsorbed post absorption, the nucleic acid obtained after filtration or adsorbed nucleic acid
Again by follow-up rinsing and eluting thus reach extract or the purpose of purification.
During realizing the present invention, inventor finds that prior art at least there is problems in that
During existing column method purification of nucleic acid, need to transfer to pure in test tube by nucleic acid solution to be purified
Changing in post, the transfer process of this nucleic acid solution it is frequently necessary to complete manually, but, manually move liquid and add operator
Workload, and require certain accuracy, to substitute the automatization manually realizing nucleic acid purification, need to pass through automatization
Mechanical hand or machinery pipettor complete.But, existing mechanical hand or machinery pipettor make self-reacting device volume bigger
Heavier mass, and mechanical hand or machinery pipettor improve the price of self-reacting device, is unfavorable for nucleic acid purification.
Summary of the invention
Needing to use mechanical hand or the problem of machinery pipettor to solve self-reacting device in prior art, the present invention is real
Execute example and provide a kind of nucleic acid purification system.Described technical scheme is as follows:
Embodiments providing a kind of nucleic acid purification system, described nucleic acid purification system includes: nucleic acid purification post, institute
The top stating nucleic acid purification post is provided with purification column inlet, and the bottom of described nucleic acid purification post is provided with purification column liquid outlet,
Being disposed with nucleic acid extraction film in described nucleic acid purification post, described nucleic acid extraction film covers on described purification column liquid outlet, described
Nucleic acid purification system includes: liquid selective pass through device, described liquid selective through device the most waterproof and
Passing through the most for liquid, described liquid selective is arranged in described nucleic acid purification post through device, described liquid
Selectivity passes through device between described purification column inlet and described nucleic acid extraction film.
Specifically, described liquid selective is through device for can wear out film by physics, and described nucleic acid purification system also includes: make
Described liquid selective is through the film breaking unit ruptured.
Further, described film breaking unit be rupture of membranes rod, malleation provide device or negative pressure to provide device.
Further, described system also includes that support ring, described support ring are sealingly mounted at the interior of described nucleic acid purification post
On wall, described liquid selective is fixed in described support ring through device.
Further, described liquid selective includes outer shroud and detachable flashing through device, and described outer ring seal is pacified
Being contained on the inwall of described nucleic acid purification post, described detachable flashing is installed on described outer shroud inwall, and described detachably
Flashing is connected with described outer ring seal, and described nucleic acid purification system also includes: for disclosing out the rod of described detachable flashing
Son.
Described liquid selective includes through device: support film and waterproof ventilated membrane, described support film phonograph seal is arranged on institute
On the inwall of the described nucleic acid purification post stating the top of nucleic acid extraction film, described support film is provided with the first water hole, described anti-
Water ventilated membrane is attached on described support film, and under normal pressure, described waterproof ventilated membrane is waterproof and ventilative, under malleation or negative pressure, described
Waterproof ventilated membrane is permeable and ventilative.
Further, described nucleic acid purification system also includes providing the malleation of malleation to provide device for described waterproof ventilated membrane
Or the vacuum equipment of negative pressure is provided.
Specifically, described malleation provides device include the first housing and be positioned at the gas injection dress of described first enclosure interior
Putting, described first housing offers gas inlet, described gas inlet can be tightly connected with described purification column inlet.
Specifically, described vacuum equipment includes the second housing, test tube, test tube rack and is positioned at bleeding of described second housing
Device, the top of described second housing is provided with the feed pathway removably connected with described purification column liquid outlet, described test tube
Frame is arranged in described second housing, and described test tube is arranged on described test tube rack and is positioned at the lower section of described feed pathway.
Specifically, described system also includes that mounting post, the top of described mounting post are provided with inlet, described mounting post
Bottom is provided with liquid outlet, and described mounting post is set in described nucleic acid purification post and is installed on described purification column inlet,
The liquid outlet of described mounting post is positioned at the top of described nucleic acid extraction film, and described liquid selective is arranged on described peace through device
Dress post is interior and covers on the liquid outlet of described mounting post.
The technical scheme that the embodiment of the present invention provides has the benefit that system that the embodiment of the present invention provides is at liquid
Need not manually complete the operation of falling liquid during body transfer, it also avoid use automated mechanical hands simultaneously or machinery moves liquid
Device, this system cost is low, compact, meanwhile, through device, the pipetting processes of nucleic acid purification is had by liquid selective
Have and simplify and the feature of automatization.
Accompanying drawing explanation
For the technical scheme being illustrated more clearly that in the embodiment of the present invention, in embodiment being described below required for make
Accompanying drawing be briefly described, it should be apparent that, below describe in accompanying drawing be only some embodiments of the present invention, for
From the point of view of those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to obtain other according to these accompanying drawings
Accompanying drawing.
Fig. 1 be the embodiment of the present invention one provide with lid can physics wear out film coordinate rupture of membranes rod use state
Figure；
Fig. 2 is the outer shroud that provides of the embodiment of the present invention one and the structural representation that matches of detachable flashing；
Fig. 3 is that the lid that supports that the embodiment of the present invention one provides passes through, with liquid selective, the structural representation that device coordinates；
Fig. 4 is the structural representation of the nucleic acid purification system with vacuum equipment that the embodiment of the present invention one provides；
Fig. 5 is the structural representation of the nucleic acid purification system providing device with malleation that the embodiment of the present invention one provides；
Fig. 6 be the embodiment of the present invention two provide can wear out the structure that film is arranged in the nucleic acid purification system of mounting post by physics
Schematic diagram；
Fig. 7 is that the structure of the nucleic acid purification system that the waterproof ventilated membrane that the embodiment of the present invention two provides is arranged in mounting post is shown
It is intended to.
Detailed description of the invention
For making the object, technical solutions and advantages of the present invention clearer, below in conjunction with accompanying drawing to embodiment party of the present invention
Formula is described in further detail.
Embodiment one
The embodiment of the present invention one provides a kind of nucleic acid purification system, as it is shown in figure 1, this nucleic acid purification system includes: core
Acid purification column 1, the top of nucleic acid purification post 1 is provided with purification column inlet 1c, and the bottom of nucleic acid purification post 1 is provided with purification column
Liquid outlet 1a, is disposed with nucleic acid extraction film 1b, nucleic acid extraction film 1b and covers on purification column liquid outlet 1a in nucleic acid purification post 1,
This nucleic acid purification system also includes: liquid selective pass through device, liquid selective through device the most waterproof and
Passing through the most for liquid, liquid selective is arranged in nucleic acid purification post 1 through device, and liquid selective is through dress
Setting between purification column inlet 1c and nucleic acid extraction film 1b.
Specifically, liquid selective is through device for can wear out film 4 by physics, and this nucleic acid purification system can also include: makes
The film breaking unit that liquid selective ruptures through device.
Further, film breaking unit can be that rupture of membranes rod 5, malleation offer device 6 or negative pressure provide device 7.When rupture of membranes fills
When being set to rupture of membranes rod 5, in process of the test, rupture of membranes rod 5 can make can to wear out film 4 and rupture by physics, makes to wear out above film 4 by physics
Liquid is the dirtiest.Additionally, malleation provides device 6 can be that this system provides malleation, negative pressure provides device 7 can be that this system carries
For negative pressure, under malleation or negative pressure, film 4 can be worn out rupture by physics, make liquid dirty.Additionally, film 4 can be worn out can be by physics
Thin plastics or highdensity waterproof paper.
Specifically, as in figure 2 it is shown, liquid selective includes outer shroud 4a and detachable flashing 4b, outer shroud 4a through device
Being sealingly mounted on the inwall of nucleic acid purification post 1, detachable flashing 4b is installed on outer shroud 4a inwall, and detachable flashing
4b is tightly connected with outer shroud 4a, and nucleic acid purification system also includes: for disclosing out the rod of detachable flashing 4b.Wherein, removable
Unload the edge of flashing 4b to only have sub-fraction with outer shroud 4a and be connected, or, detachable flashing 4b and outer shroud 4a is permissible
Being connected by connector, after detachable flashing 4b is disclosed out by rod, detachable flashing 4b does not falls out to nucleic acid extraction
On film 1b, thus affect nucleic acid extraction film 1b and extract nucleic acid.Detachable flashing 4b can be disclosed out from outer shroud 4a by this rod, makes
Liquid above detachable flashing 4b is the dirtiest.In the present embodiment, the material of detachable flashing 4b can be to mould
Material, the material of outer shroud 4a can be identical with the material of detachable flashing 4b.
Specifically, liquid selective is the most waterproof and pass through for liquid under imposing a condition through device, and liquid selects
Selecting property is arranged in nucleic acid purification post 1 through device, and liquid selective is positioned at purification column inlet 1c through device and carries with nucleic acid
Take between film 1b.
Further, this nucleic acid purification system can also include that support ring 8, support ring 8 are sealingly mounted at nucleic acid purification post 1
Inwall on, liquid selective is fixed in support ring 8 through device.Wherein, support ring 8 and liquid selective can through device
For identical material.
Further, as it is shown on figure 3, this nucleic acid purification system can also include supporting lid 9, support lid 9 and be sealingly mounted at
On the inwall of nucleic acid purification post 1, supporting and offer the second water hole on lid 9, liquid selective is fixed on support lid 9 through device
Going up and cover on the second water hole, the top supporting lid 9 is provided with clamp 9a, clamp 9a and supports lid 9 by liquid selective
It is clipped in the middle through device.This support lid 9 is used for supporting liquid selective and passes through device, and supporting lid 9 cooperation clamp 9a can be by liquid
Body selectivity is stably fixed in nucleic acid purification post 1 through device, and this support lid 9 can be the various material such as plastics, liquid
Selectivity can be can to wear out film 4 by physics through device.
Specifically, as shown in Figure 4 and Figure 5, liquid selective may include that support film 2 and waterproof ventilated membrane through device
3, on the inwall of the nucleic acid purification post 1 that support film 2 is sealingly mounted at the top of nucleic acid extraction film 1b, support film 2 is provided with first
Water hole, waterproof ventilated membrane 3 is attached on support film 2, and under the first state of normal pressure, waterproof ventilated membrane 3 is waterproof and ventilative,
Under second state of malleation or negative pressure, waterproof ventilated membrane 3 is permeable and ventilative.Wherein, the first water hole can be multiple, and uniformly
It is arranged on support film 2 position covered by waterproof ventilated membrane 3.In this application, waterproof ventilated membrane 3 can be polytetrafluoroethyl-ne
Alkene.
Further, this nucleic acid purification system can also include providing the malleation of malleation to provide device 6 for waterproof ventilated membrane 3
(see Fig. 5) or the vacuum equipment 7 (see Fig. 4) of offer negative pressure.
Specifically, as it is shown in figure 5, malleation provides device 6 can include the first housing 6a and be positioned at inside the first housing 6a
Gas injection apparatus (not shown), the first housing 6 offers gas inlet, gas inlet can be with purification column feed liquor
Mouth 1c is tightly connected.This malleation provides device 6 can be that system provides malleation.Additionally, when liquid selective, through device, include can
When physics wears out film 4, nucleic acid purification system can use this malleation to provide device 6, and this malleation provides device 6 can seal installation
With on purification column inlet 1c, for providing malleation to provide device for nucleic acid purification system, realize under positive pressure wearing by physics
Rupturing of rupture of membranes 4.
Specifically, as shown in Figure 4, vacuum equipment 7 can include the second housing 7a, test tube 7b, test tube rack 7c and be positioned at
The air extractor (not shown) of 7a in two housings, the top of the second housing 7a is provided with and detachably connects with purification column liquid outlet 1a
The feed pathway connect, test tube rack 7c is arranged in the second housing 7a, and test tube 7b is arranged on test tube rack 7c and is positioned at feed pathway
Lower section.Wherein, test tube 7b can be multiple, in use, different test tube 7b can be made to lay respectively at mobile test tube shelf 7c
The lower section of feed pathway, for collecting the different liquid of 1a, as molten in the rinsing solution of purification of nucleic acid or the good nucleic acid of purification
Liquid.Wherein, the inwall of feed pathway can arrange silica gel stopple, be used for connecting purification column liquid outlet 1a, additionally, feed pathway
Female thread can be provided with on inwall, the outer wall of purification column liquid outlet 1a can be provided with and the female thread on the inwall of feed pathway
The external screw thread matched.Air in nucleic acid purification post 1 can be extracted out and make this intrasystem pressure become negative by this vacuum equipment 7
Pressure, it is achieved the function of negative pressure is provided for system.When liquid selective through device include can physics wear out film 4 time, nucleic acid purification
System can use vacuum equipment 7 to provide device as negative pressure, and this vacuum equipment 7 can be sealingly mounted at and purification column liquid outlet
On 1a, for providing negative pressure for nucleic acid purification system, realize under negative pressure to wear out rupturing of film 4 by physics.
Positive pressure pump (not shown) can also be used to provide malleation for waterproof ventilated membrane 3 additionally, this goes back nucleic acid purification system,
Using vacuum pump (not shown) to provide negative pressure for waterproof ventilated membrane 3, specifically, positive pressure pump is by sealing pipe and purification column feed liquor
Mouth 1c connection, vacuum pump connects with purification column liquid outlet 1a by sealing pipe.
Specifically, nucleic acid extraction film 1b can be adsorbed film or filter membrane.
Following is a brief introduction of the operation principle of the nucleic acid purification system that the embodiment of the present invention provides:
When nucleic acid purification system include can physics wear out film 4 time, user by liquid cell and various chemical reagent (as carefully
Cellular lysate liquid, nucleic acid release liquid etc.) join and can wear out on film 4 by physics, stand after mixing to liquid cell rupture, intracellular
Nucleic acid is discharged in solution thus forms nucleic acid solution to be purified, makes to wear out film 4 and rupture by physics by film breaking unit, can thing
Reason is worn out after film 4 ruptures so that nucleic acid solution to be purified is dirty.When nucleic acid extraction film 1b is filter membrane, core to be purified
Impurity in acid solution is filtered, and nucleic acid is then by filter membrane, and when nucleic acid extraction film 1b is adsorbed film, nucleic acid is attracted to
On adsorbed film, then by nucleic acid after purification can be obtained after adding the reagent such as rinsing liquid and eluent in nucleic acid purification post 1, from
And achieve the purpose of nucleic acid extraction purification.
When nucleic acid purification system includes outer shroud 4a and detachable flashing 4b, user is by liquid cell and various chemistry
Reagent (such as cell pyrolysis liquid etc.) joins on outer shroud 4a and detachable flashing 4b, stands to liquid cell and rupture after mixing,
Intracellular nucleic acid is discharged in solution thus forms nucleic acid solution to be purified, by rod by detachable flashing 4b by outward
Disclose out on ring 4a, make nucleic acid solution to be purified dirty, then realize nucleic acid extraction purification further by nucleic acid extraction film 1b
Purpose.
When nucleic acid purification system includes waterproof ventilated membrane 3, under normal pressure, user is by liquid cell and various chemical reagent
(such as cell pyrolysis liquid, nucleic acid release liquid etc.) joins on waterproof ventilated membrane 3, stand after mixing to liquid cell rupture, cell
Interior nucleic acid is discharged in solution thus forms nucleic acid solution to be purified, when waterproof ventilated membrane 3 is in the first shape of normal pressure
Duct Guan Bi under state, on waterproof ventilated membrane 3 so that this waterproof ventilated membrane 3 has function waterproof, ventilative, i.e. waterproof and breathable
Film 3 can prevent the nucleic acid solution above it dirty, when, under the second state that waterproof ventilated membrane 3 is in malleation or negative pressure, this is waterproof
Duct on ventilated membrane 3 is opened under the effect of positive/negative pressure so that this waterproof ventilated membrane 3 has permeable function, thus
Make the materials such as nucleic acid can be dirty through waterproof ventilated membrane 3, device 6 can be provided to make nucleic acid purification post by malleation in the present embodiment
The internal medium of 1 is positive pressure environment, or makes the internal medium of nucleic acid purification post 1 be subnormal ambient by vacuum equipment 7, specifically
Ground, when using malleation to provide device 6, waterproof ventilated membrane 3 can be breathed freely and permeable, now under malleation provides the effect of device 6
Nucleic acid solution to be purified flow on nucleic acid extraction film 1b through waterproof ventilated membrane 3, and subsequent step is as above.
The nucleic acid purification system that the embodiment of the present invention provides, in the transfer process of nucleic acid solution, this nucleic acid purification post is in
Atmospheric pressure environment, liquid cell and various chemical reagent (such as cell pyrolysis liquid, nucleic acid release liquid etc.) join nucleic acid purification post
Liquid selective through on device, stand after mixing to liquid cell rupture, intracellular nucleic acid is discharged in solution thus shape
Become nucleic acid solution to be purified, by under imposing a condition, make nucleic acid pass through liquid selectivity and pass through device, so that nucleic acid etc.
Material can be dirty, flow to carry out on nucleic acid extraction film the extraction of nucleic acid, this system nucleic acid solution shift during without hands
Move the operation of falling liquid, it also avoid use automated mechanical hands or machinery shifting liquid by liquid selective through device simultaneously
Device, this system cost is low, compact, simultaneously so that the pipetting processes of nucleic acid purification has to be simplified and the feature of automatization.
Embodiment two
The embodiment of the present invention two provides another kind of nucleic acid purification system, and as shown in Figure 6 and Figure 7, this system can be with reality
Based on executing example one, on the basis of the nucleic acid purification system that embodiment one provides, increase mounting post 11, the top of mounting post 11
Being provided with inlet, the bottom of mounting post 11 is provided with liquid outlet 11a, in mounting post 11 is set in nucleic acid purification post 1 and block dress
On purification column inlet 1c, liquid outlet 11a is positioned at the top of nucleic acid extraction film 1b, and liquid selective is arranged on peace through device
Dress post 11 is interior and covers on the liquid outlet of mounting post 11.Mounting post 11 can be removably mounted in nucleic acid purification post 1, when
After loading liquid in mounting post 11, mounting post 11 can be separated with nucleic acid purification post 1, rock or stir the liquid in mounting post 11
Body realizes the purpose of the liquid blending in mounting post 11.
Further, the outer wall of mounting post 11 is provided with protruding 11b, protruding 11b and is installed on purification column inlet 1c.Should
Protruding 11b can be by the purification column inlet 1c being arranged in nucleic acid purification post 1 stable for mounting post 11.
The operation principle of the nucleic acid purification system that embodiment two provides sees the nucleic acid purification system of embodiment one offer
Operation principle.
Embodiments provide a kind of nucleic acid purification system, in the first state, by liquid cell and various chemistry
Reagent (such as cell pyrolysis liquid, nucleic acid release liquid etc.) joins in mounting post, and the nucleic acid stood after mixing to liquid cell is released
Put thus form nucleic acid solution to be purified, by under a second condition, make nucleic acid pass through liquid selectivity and pass through device, thus
Making the materials such as nucleic acid dirty, flow to carry out on nucleic acid extraction film the extraction of nucleic acid, this system is during nucleic acid solution shifts
Without manually completing the operation of falling liquid, it also avoid use automated mechanical hands or machine by liquid selective through device simultaneously
Tool pipettor, this system cost is low, compact, simultaneously so that the pipetting processes of nucleic acid purification has simplification and automatization
Feature.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all spirit in the present invention and
Within principle, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (10)
1. a nucleic acid purification system, described nucleic acid purification system includes: nucleic acid purification post (1), described nucleic acid purification post (1)
Top is provided with purification column inlet (1c), and the bottom of described nucleic acid purification post (1) is provided with purification column liquid outlet (1a), described
Being disposed with nucleic acid extraction film (1b) in nucleic acid purification post (1), described nucleic acid extraction film (1b) covers at described purification column liquid outlet
(1a) on, it is characterised in that described nucleic acid purification system also includes:
Liquid selective passes through device, and described liquid selective is the most waterproof and supply in the second condition through device
Penetration by liquid, described liquid selective is arranged in described nucleic acid purification post (1) through device, and described liquid selective is through dress
Setting between described purification column inlet (1c) and described nucleic acid extraction film (1b).
Nucleic acid purification system the most according to claim 1, it is characterised in that described liquid selective is can thing through device
Reason wears out film (4), and described nucleic acid purification system also includes: make the film breaking unit that described liquid selective ruptures through device.
Nucleic acid purification system the most according to claim 2, it is characterised in that described film breaking unit is rupture of membranes rod (5), malleation
Device (6) or negative pressure is provided to provide device (7).
Nucleic acid purification system the most according to claim 2, it is characterised in that described nucleic acid purification system also includes support ring
(8), described support ring (8) is sealingly mounted on the inwall of described nucleic acid purification post (1), and described liquid selective is solid through device
It is scheduled in described support ring (8).
Nucleic acid purification system the most according to claim 1, it is characterised in that outside described liquid selective includes through device
Ring (4a) and detachable flashing (4b), described outer shroud (4a) is sealingly mounted on the inwall of described nucleic acid purification post (1), described
Detachable flashing (4b) is installed on described outer shroud (4a) inwall, and described detachable flashing (4b) and described outer shroud (4a)
Being tightly connected, described nucleic acid purification system also includes: be used for disclosing out the rod of described detachable flashing (4b).
Nucleic acid purification system the most according to claim 1, it is characterised in that described liquid selective includes through device:
Support film (2) and waterproof ventilated membrane (3), described support film (2) is sealingly mounted at the institute of the top of described nucleic acid extraction film (1b)
Stating on the inwall of nucleic acid purification post (1), described support film (2) is provided with the first water hole, and described waterproof ventilated membrane (3) is attached at
On described support film (2), under normal pressure, described waterproof ventilated membrane (3) is waterproof and ventilative, under malleation or negative pressure, and described waterproof and breathable
Film (3) is permeable and ventilative.
Nucleic acid purification system the most according to claim 6, it is characterised in that described nucleic acid purification system also includes for described
Waterproof ventilated membrane (3) provides the malleation of malleation provide device (6) or provide the vacuum equipment (7) of negative pressure.
8. according to the nucleic acid purification system described in claim 3 or 7, it is characterised in that described malleation provides device (6) to include the
One housing (6a) and be positioned at the gas injection apparatus that described first housing (6a) is internal, described first housing (6a) offers gas
Body inlet, described gas inlet can be tightly connected with described purification column inlet (1c).
9. according to the nucleic acid purification system described in claim 3 or 7, it is characterised in that described vacuum equipment (7) includes the second shell
Body (7a), test tube (7b), test tube rack (7c) and be positioned at the air extractor of described second housing (7a), described second housing (7a)
Top be provided with the feed pathway removably connected with described purification column liquid outlet (1a), described test tube rack (7c) is arranged in institute
Stating in the second housing (7a), described test tube (7b) is arranged on described test tube rack (7c) and is positioned at the lower section of described feed pathway.
Nucleic acid purification system the most according to claim 1, it is characterised in that described nucleic acid purification system also includes installing
Post (11), the top of described mounting post (11) is provided with inlet, and the bottom of described mounting post (11) is provided with liquid outlet
(11a), described mounting post (11) is set in described nucleic acid purification post (1) and is installed on described purification column inlet (1c),
The liquid outlet (11a) of described mounting post (11) is positioned at the top of described nucleic acid extraction film (1b), and described liquid selective is through dress
Put in being arranged on described mounting post (11) and cover on the liquid outlet (11a) of described mounting post (11).
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| CN201610442151.1A CN106086003A (en) | 2016-06-17 | 2016-06-17 | A kind of nucleic acid purification system |
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Effective date of registration: 20171211 Address after: No. 613, No. 818 B9, No. 818, East Lake new technology open area, East Lake new technology open area, Hubei Applicant after: Wuhan medical biotech Co., Ltd. Address before: 430100 23 3 unit 502, Hawaii water Ying Xiang embankment, Hawaii, Caidian District, Wuhan, Hubei Applicant before: Yi Chuli |
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Application publication date: 20161109 |