CN113908588A - Full-automatic solid phase extraction instrument - Google Patents
Full-automatic solid phase extraction instrument Download PDFInfo
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- CN113908588A CN113908588A CN202111112387.6A CN202111112387A CN113908588A CN 113908588 A CN113908588 A CN 113908588A CN 202111112387 A CN202111112387 A CN 202111112387A CN 113908588 A CN113908588 A CN 113908588A
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- solid phase
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- purification device
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- 238000002414 normal-phase solid-phase extraction Methods 0.000 title claims abstract description 26
- 238000000746 purification Methods 0.000 claims abstract description 63
- 238000012545 processing Methods 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 15
- 238000009434 installation Methods 0.000 claims abstract description 14
- 239000007790 solid phase Substances 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 17
- 238000011084 recovery Methods 0.000 claims description 13
- 238000002347 injection Methods 0.000 claims description 8
- 239000007924 injection Substances 0.000 claims description 8
- 238000004064 recycling Methods 0.000 claims description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- 238000011068 loading method Methods 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- 230000006920 protein precipitation Effects 0.000 claims description 3
- 238000007664 blowing Methods 0.000 claims description 2
- 238000011282 treatment Methods 0.000 abstract description 17
- 239000000523 sample Substances 0.000 description 64
- 238000010828 elution Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 239000002904 solvent Substances 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 210000001503 joint Anatomy 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000004913 activation Effects 0.000 description 1
- 238000001994 activation Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 238000004460 liquid liquid chromatography Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000001172 liquid--solid extraction Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 125000002560 nitrile group Chemical group 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/12—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the preparation of the feed
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N2030/022—Column chromatography characterised by the kind of separation mechanism
- G01N2030/027—Liquid chromatography
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Extraction Or Liquid Replacement (AREA)
Abstract
The invention provides a full-automatic solid phase extraction instrument, which comprises a main body, wherein a cavity is arranged in the main body, a three-dimensional motion module which is used for fixing a sample purification device and can move in the cavity in a three-dimensional mode is arranged in the cavity, the main body is provided with a first installation position communicated with the cavity, the first installation position is used for fixing a first processing module, and when the sample purification device is located at a first position, the first processing module is used for processing the sample purification device. Compared with the prior art, the full-automatic solid phase extraction apparatus provided by the invention has the advantages that the sample purification device is moved to the first position through the three-dimensional movement module to complete the treatment of the sample purification device by the first treatment module, the movement of the first treatment module is not required in the process, so that a complex mechanical device is not required to be arranged on the first treatment module, the first treatment module can be fixed through the first installation position arranged on the main body, and meanwhile, the first treatment module can be conveniently detached.
Description
Technical Field
The invention relates to the field of sample pretreatment, in particular to a full-automatic solid phase extraction instrument.
Background
Solid Phase Extraction (SPE) is a sample pretreatment technology developed from the mid-eighties. Developed by combining liquid-solid extraction and liquid chromatography techniques. The method is mainly used for separating, purifying and enriching samples. The main purpose is to reduce the sample matrix interference and improve the detection sensitivity.
The SPE technology is based on the liquid-solid phase chromatography theory, enriches, separates and purifies a sample by adopting selective adsorption and selective elution, is a physical extraction process comprising a liquid phase and a solid phase, and can also be approximately regarded as a simple chromatographic process. SPE is a liquid chromatography separation principle using selective adsorption and selective elution. The more common method is to make the liquid sample solution pass through the adsorbent to retain the tested substance, then to select the solvent with proper strength to wash out the impurity, then to use a small amount of solvent to quickly elute the tested substance, thus to achieve the purpose of quick separation, purification and concentration. Or adsorbing the interfering impurities and the substance to be detected simultaneously, and then selectively eluting the substance to be detected by using a proper solvent. The extractant of the solid phase extraction method is solid, and the working principle is based on: the components to be detected and the coexisting interference components in the water sample have different acting forces on the solid phase extracting agent, so that the components are separated from each other. The solid phase extracting agent is special stuffing containing C18 or C8, nitrile group, amino group, etc.
A full-automatic solid phase extractor is a set of solid phase extractor which is derived and developed by a liquid processing platform and can automatically run a solid phase extraction method under the unattended condition. Comprises the steps of activation, loading, washing and elution of solid phase extraction, and switching of samples. Present full-automatic solid phase extraction appearance, be provided with the malleation usually, annotate liquid and nitrogen blow the module, and fixed sample purification device, thereby be connected with sample purification device through the removal of malleation module and handle and make waste liquid or eluant outflow to sample purification device, remove through annotating the liquid module and sample purification device cooperation (annotate the liquid mouth of notes liquid module and go deep into sample purification device's cavity promptly) add the leacheate in order to carry out the drip washing processing to sample purification device, the full-automatic solid phase extraction appearance of prior art needs the cooperation of many sets of manipulators, the structure is complicated, it is inconvenient to maintain.
Therefore, it is necessary to provide a fully automatic solid phase extraction apparatus with a simpler structure and convenient maintenance.
Disclosure of Invention
In order to solve the problems, the invention provides a full-automatic solid phase extraction instrument which can perform full-automatic solid phase extraction on a sample, has a simpler structure and is convenient to maintain.
The invention discloses a full-automatic solid phase extraction instrument, which comprises a main body, wherein a cavity is arranged in the main body, a three-dimensional motion module which is used for fixing a sample purification device and can move in the cavity in a three-dimensional mode is arranged in the cavity, the main body is provided with a first installation position communicated with the cavity, the first installation position is used for fixing a first processing module, and when the sample purification device is located at the first position, the first processing module processes the sample purification device.
Compared with the prior art, the full-automatic solid phase extraction apparatus provided by the invention has the advantages that the sample purification device is moved to the first position through the three-dimensional movement module to complete the treatment of the sample purification device by the first treatment module, the movement of the first treatment module is not required in the process, so that a complex mechanical device is not required to be arranged on the first treatment module, the first treatment module can be fixed through the first installation position arranged on the main body, the first treatment module can be conveniently detached, various treatment modules can be arranged according to the use requirement, and the customization is convenient.
Furthermore, the three-dimensional motion module comprises a vertically arranged telescopic rod, a cross rod capable of rotating around the horizontal direction is arranged on the telescopic rod, and a sample purification device is fixed at one end of the cross rod. Through the cooperation of above-mentioned telescopic link and horizontal pole, can make sample purifier can carry out three-dimensional movement in horizontal direction and vertical direction for sample purifier can form the cooperation with first processing module.
Furthermore, the three-dimensional motion module comprises a chassis capable of horizontally moving, a lifting platform is arranged on the chassis, and a sample purification device is fixed on the lifting platform. Through the cooperation of above-mentioned chassis and elevating platform, can make sample purifier can carry out three-dimensional movement in horizontal direction and vertical direction for sample purifier can form the cooperation with first processing module.
Further, the main part is equipped with the second installation position of intercommunication inner chamber, and the second installation position is used for fixed second processing module, and sample purifier is when the second position, and second processing module handles sample purifier. By providing a second mounting location on the body, it is possible to allow the second processing module to be secured so that multiple or multiple processes can be performed on the sample purification apparatus.
Further, the first treatment module and the second treatment module are independently selected from one or two of a positive pressure module, a liquid injection module and a nitrogen blowing module. According to the use requirement, the combination of different processing modules can be selected, for example, the positive pressure module can be selected for the first processing module, and the liquid injection module can be selected for the second processing module, so that the steps of positive pressure processing, leaching, elution and the like for the sample purification device can be realized.
Furthermore, the first processing module and the first installation position are fixed through any one structure of interference fit, clamping fit or buckling fit. Through above-mentioned detachable fixed mode, can conveniently dismantle first processing module.
Further, the lumen contains at least one sample inlet that allows the sample purification device to pass through. So, operating personnel need not to stretch into the hand and can accomplish placing and taking out of sample purifier in the inner chamber, can guarantee operating personnel's safety.
Furthermore, a recovery frame for loading a recovery device is arranged on the three-dimensional motion module, and the recovery device is in butt joint with the lower end of the sample purification device at a third position. The recovery device can recover the liquid flowing out from the sample purification device.
Further, the recovery device is one of a recovery cassette, a 6-well plate, a 12-well plate, a 24-well plate and a 96-well plate.
Further, the sample purification device is one of a solid phase extraction column or a protein precipitation plate.
Drawings
FIG. 1 is a side cross-sectional view of an embodiment of the fully automatic solid phase extractor of the present invention.
Fig. 2 is a side sectional view of a sample purification device of an embodiment of the fully automatic solid phase extractor of the present invention in a first position.
FIG. 3 is a top view of a sample purification device of an embodiment of the fully automatic solid phase extractor of the present invention in a first position.
FIG. 4 is a top view of an embodiment of the fully automatic solid phase extractor of the present invention in a sample loading/unloading state.
FIG. 5 is a side cross-sectional view of another embodiment of the fully automatic solid phase extractor of the present invention.
FIG. 6 is a side cross-sectional view of a recovery frame of another embodiment of the fully automatic solid phase extractor of the present invention in a third position.
FIG. 7 is a side cross-sectional view of a sample purification apparatus of another embodiment of the fully automatic solid phase extractor of the present invention in a second position.
FIG. 8 is a top view of a sample purification device in a second position according to another embodiment of the fully automatic solid phase extractor of the present invention.
Description of the element reference numerals
100-full-automatic solid phase extraction instrument; 1-a body; 11-a first mounting location; 13-a second mounting location; 2-a cavity; 3-a three-dimensional motion module; 31-a telescopic rod; 33-a cross-bar; 4-a sample purification device; 5-a processing module; 51-a first processing module; 53-a second processing module; 6-a recovery frame; 7-recovery device.
Detailed Description
To explain technical contents, structural features, and technical effects of the present invention in detail, the following detailed description is given with reference to the accompanying drawings in conjunction with specific embodiments.
Referring to fig. 1-3, a full-automatic solid phase extraction apparatus 100 of the present embodiment includes a main body 1 having a cavity 2 therein, a three-dimensional motion module 3 for fixing a sample purification device 4 is disposed in the cavity 2, the three-dimensional motion module 3 can move three-dimensionally in the cavity 2, a first mounting position 11 communicating with the cavity 2 is disposed on the main body 1, and the first mounting position 11 is used for fixing a first processing module 51. When the sample purification apparatus 4 is moved to the first position shown in fig. 2 (the positive pressure processing apparatus is in sealing connection with the upper end of the sample purification apparatus 4 in this embodiment), the top view is shown in fig. 3, the sample purification apparatus 4 is overlapped with the first processing module 51 in the horizontal position, and the first processing module 51 installed at the first installation position 11 processes the sample purification apparatus 4. The recovery unit 7 in this embodiment is a 96-well plate, but it is needless to say that one of a recovery cassette, a 6-well plate, a 12-well plate and a 24-well plate may be selected as required. The sample purification device 4 in this embodiment is a 96-well solid phase extraction column, but a protein precipitation plate or other sample purification device 4 may be used.
Referring to fig. 1-3, the three-dimensional motion module 3 in this embodiment includes a vertically disposed telescopic rod 31, a horizontal rod 33 is disposed on the telescopic rod 31 and rotates in a horizontal direction, and the sample purification device module 4 is fixed to one end of the horizontal rod 33. The sample purification device 4 can be moved in the vertical direction by the telescopic rod 31, and the sample purification device 4 can be rotated in the horizontal direction by the cross rod 33. Of course, in other embodiments, the three-dimensional motion module 3 may comprise a horizontally displaceable chassis on which a stage is provided, on which the sample purification apparatus 4 is fixed. As long as the movement of the sample purification apparatus 4 in the three-dimensional direction can be achieved.
Referring to fig. 1-3, the fully automatic solid phase extractor 100 of the present embodiment includes a first purification module 51 and a second purification module 53, wherein the first purification module 51 is a positive pressure module and is fixed to the first installation position 51 by snap fit so as to pass through the inner cavity 2, and the second purification module 53 is a liquid injection module and is fixed to the second installation position 53 by snap fit so as to pass through the inner cavity 2, and of course, other modules can be selected as required for the first purification module 51 and the second purification module 53 and are fixed to the main body 1 by other reversible connection methods. Since the first and second cleaning modules 51 and 53 do not need to be provided with a robot structure, the first and second cleaning modules 51 and 53 can be easily mounted/dismounted.
Referring to fig. 4, the sample purification apparatus 4 is not completely overlapped with the first process module 51 or the second process module 53 in the horizontal direction, and the sample purification apparatus 4 can be put into/removed from the cavity 2 through a sample inlet (not shown) provided on the cavity 2. So that the sample can be conveniently placed/removed.
Referring to fig. 5-6, in another embodiment of the fully automatic solid phase extractor 100 of the present invention, a recycling rack 6 for loading a recycling device 7 is disposed in the inner cavity 2, and the recycling device 7 is abutted against the lower end of the sample purification device 4 at a third position (shown in fig. 6) by the movement of the recycling rack 6. Thus, the waste liquid generated after the sample purification device 4 is subjected to positive pressure treatment can be collected.
Referring to fig. 7-8, in another embodiment of the fully automatic solid phase extractor 100 of the present invention, the sample purification device 4 is located at the second position, the sample purification device 4 and the second processing module 53 are in butt joint (i.e. the liquid injection port of the liquid injection module enters the cavity of the sample purification device 4), the top view is shown in fig. 8, the sample purification device 4 and the second processing module 53 are overlapped in the horizontal position, and the second processing module 53 performs processing on the sample purification device 4 (i.e. the liquid injection module performs liquid injection on the sample purification device 4). Thus, the fully automatic solid phase extractor 100 of the present invention can perform a variety of treatments on a sample purification device.
The above disclosure is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the scope of the present invention, therefore, the present invention is not limited by the appended claims.
Claims (10)
1. The utility model provides a full-automatic solid phase extraction appearance, its characterized in that contains the main part that is equipped with the cavity in, be equipped with in the cavity and be used for fixed sample purifier and can in the three-dimensional motion module of intracavity three-dimensional removal, the main part is equipped with the first installation position of intercommunication inner chamber, first installation position is used for fixed first processing module, sample purifier when first position, first processing module is to sample purifier handles.
2. The automatic solid phase extraction apparatus of claim 1, wherein the three-dimensional motion module comprises a vertically disposed telescopic rod, the telescopic rod is provided with a horizontal rod capable of rotating around a horizontal direction, and one end of the horizontal rod is fixed with the sample purification device.
3. The automatic solid phase extractor of claim 1, wherein the three-dimensional motion module comprises a horizontally displaceable base plate, and a lifting platform is disposed on the base plate, and the sample purification device is fixed on the lifting platform.
4. The automatic solid phase extractor of claim 1, wherein the main body has a second mounting position connected to the inner cavity, the second mounting position is used for fixing a second processing module, and the second processing module processes the sample purifying device when the sample purifying device is at a second position.
5. The fully automatic solid phase extractor of claim 4, wherein the first processing module and the second processing module are independently selected from one or two of a positive pressure module, a liquid injection module and a nitrogen blowing module.
6. The automatic solid phase extractor of claim 1, wherein the first processing module is fixed to the first mounting portion by any one of interference fit, snap fit, or snap fit.
7. The fully automated solid phase extractor of claim 1, wherein the internal chamber comprises at least one sample inlet that allows the sample purification device to pass through.
8. The fully automatic solid phase extractor of claim 1, wherein the inner chamber is provided with a recycling rack for loading a recycling device, and the recycling device is butted with the lower end of the sample purifying device at a third position by moving the recycling rack.
9. The fully automatic solid phase extractor of claim 8, wherein the recovery device is one of a recovery cartridge, a 6-well plate, a 12-well plate, a 24-well plate and a 96-well plate.
10. The fully automated solid phase extractor of claim 1, wherein the sample purification device is one of a solid phase extraction column or a protein precipitation plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111112387.6A CN113908588A (en) | 2021-09-23 | 2021-09-23 | Full-automatic solid phase extraction instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111112387.6A CN113908588A (en) | 2021-09-23 | 2021-09-23 | Full-automatic solid phase extraction instrument |
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| CN113908588A true CN113908588A (en) | 2022-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111112387.6A Pending CN113908588A (en) | 2021-09-23 | 2021-09-23 | Full-automatic solid phase extraction instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115586052A (en) * | 2022-09-28 | 2023-01-10 | 天津国科医疗科技发展有限公司 | Automatic processing device for sample extraction and separation |
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Application publication date: 20220111 |