CN105794551B - A method of tomato in plastic greenhouse early blight is prevented and treated using ultraviolet lamp - Google Patents
A method of tomato in plastic greenhouse early blight is prevented and treated using ultraviolet lamp Download PDFInfo
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- CN105794551B CN105794551B CN201410842162.XA CN201410842162A CN105794551B CN 105794551 B CN105794551 B CN 105794551B CN 201410842162 A CN201410842162 A CN 201410842162A CN 105794551 B CN105794551 B CN 105794551B
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Abstract
The present invention relates to technical field of pest control, specifically a kind of method using ultraviolet lamp prevention and treatment tomato in plastic greenhouse early blight.A kind of basis is designed to provide by control wavelength radiation dosage, the sprouting of tomato early blight bacterium spore, germ tube are extended, mycelia growth inhibition effect, the method for producing pollution-free vegetable.There is powerful destructive power according to germ nuclifort (DNA) and ribonucleic acid (RNA), lose viability and reproductive capacity and then eliminate germ；It can also make nucleic acid mutation, hinder its duplication, transcription block and the synthesis of protein；Or generate free radicals and can cause photo-ionisation, so as to cause the death of cell, to sterilize；Germicidal efficiency kills that effect is good, and effect is quick up to 99%.
Description
Technical field
The present invention relates to technical field of pest control, specifically a kind of side using ultraviolet lamp prevention and treatment tomato in plastic greenhouse early blight
Method.
Background technique
Annual whole world crop loss as caused by insect pest is up to 100,000,000,000 dollars.To kill agricultural pests, agricultural
Scientists are wanted to have use up method, but previous disinsection method effect is all less ideal.Chemical pesticide can make pest generate anti-medicine
Property, and pollution will also result in crops itself and ambient enviroment；Biology controls worm, although other public hazards will not be caused, by
The type for the pest controlled is restricted, some pests still " are at large ".
Ultraviolet radioactive, which acts on " one plant of germ " disease system, influences the occurrence and development of plant disease, and main includes two
Aspect: first is that the photobiology effect such as UV radiation extends the sprouting of phytopathogen spore, germ tube, mycelia growth, not of the same race
There are different response mechanisms to UV radiation for the strain of category；Second is that host plant makes UV from form, Physiology and biochemistry etc.
Response causes the research that changes of the disease resistance of plant to confirm, in, short-wave UV radiation can inhibit germ spore germination, reduce disease
Harmful disease incidence；Long wave UV radiation has inducing action to the sprouting of germ spore, and disease disease incidence can be improved according to UV and radiate water
Flat, germ infects the influence of the factors such as degree and plant resistance to environment stress kind, and to different plant disease systems, there is also differences for UV radiation
Mechanism of action.
Early blight of tomato has generation in the area such as China northeast, North China, East China, southwest, no matter main feature is
The scab on fruit, blade or stem occurs, has apparent wheel line, so also referred to as ring spot；Fruit scab often exists
Near base of fruit, often at branching, leaf portion scab occurs on mesophyll stem's scab；Early blight is that institute is infected by sporulation
It causes, in classification of fungi, belongs to Deuteromycotina Alternaria.Except occur on tomato in addition to, can also infect potato, eggplant,
The crops such as tomato, mainly infecting body is conidium.
Summary of the invention
The purpose of the present invention is to provide a kind of bases by control wavelength radiation dosage, to tomato early blight bacterium spore
It sprouts, germ tube elongation, mycelia growth inhibition effect, the method for producing pollution-free vegetable.
Specific technical solution is proposed according to above-mentioned purpose are as follows:
(1) ultraviolet generation device is set in plastic greenhouse, is killed by control wavelength and irradiation time, the intensity of irradiation
Bacterium；
The ultraviolet generation device are as follows: the UV-B fluorescent tube (240nm-340nm) of 100w；Utilize the cellulose acetate of 0.08mm
Film is coated with away 253mm wavelength components UV-C below；
The radiation intensity of the ultraviolet generation device is 100 μ W/cm2More than, the output energy of ultraviolet lamp is
100kj/m2；
The ultraviolet lamp installation method are as follows: 1.5 meters or so from the ground, be mutually perpendicular to installation ultraviolet lamp, every 20~30 is vertical
Square rice space installs one, and ultraviolet light is radiated at horizontal plane into 60~80 degree of angles；
(2) specific control method
1. the exposure dose 120000uW.s/cm received according to tomato early blight bacterium2—630000W.s/cm2And it is ultraviolet
1000 μ W/cm of generating device output intensity2, exposure dose (W.s/cm2)=irradiation time (s) × intensity (μ W/cm2), it determines
Irradiation time are as follows: -105 minutes 30 minutes, UV intensity will reduce 30%-70% to ultraviolet lamp in use, further according to
Exposure dose (W.s/cm2)=irradiation time (s) × intensity (μ W/cm2) determine required irradiation time, increase in practical applications
Add irradiation time；
2. being irradiated in tomato in plastic greenhouse early blight occurrence injury using ultraviolet lamp, because ultraviolet lamp is reached in radiation intensity
To when stablizing, needing -30 minutes 15 minutes, so ultraviolet irradiation lethal dose is at 45-135 minutes, the daily Continuous irradiation time
Less than 80 minutes, it is greater than 80 minutes, influences the conduction of signal in plant, hinders the normal vital movement of plant；So ultraviolet
During early blight of tomato prevention and treatment, irradiate 45-80 minutes daily, Continuous irradiation 5-8 inhibited to early blight of tomato
It, can kill germ；Increase the time, it is bigger to the inhibiting rate of germ；It is irradiated daily when no epidemic disease occurs 15-40 minutes, it can
It reduces in greenhouse, the generation of disease pest.
The beneficial effects of the present invention are: (1) to germ nuclifort (DNA) and ribonucleic acid (RNA) have by force
Havoc power loses viability and reproductive capacity and then eliminates germ；Can also make nucleic acid mutation, hinder its duplication, transcription block and
The synthesis of protein；Or generate free radicals and can cause photo-ionisation, so as to cause the death of cell, to sterilize；(2) germicidal efficiency
Up to 99%, kill that effect is good, and effect is quick；(3) present invention can kill other any diseases lower than tomato phytophthora germ lethal dose
Bacterium；(4) it does not develop drug resistance, can be used for a long time in plant safe dose；
Specific embodiment
Example 1, foundation foregoing invention content, propose detailed operating procedure
1, Pathogenicity of the ultraviolet light to tomato phytophthora germ
The diseased plant for acquiring tomato phytophthora germ carries out isolation and purification culture, the cause of disease purified to germ in laboratory
Bacterium；On the pathogen mycelium inoculation to potato agar culture medium of picking purifying, opened wide after being cultivated 48 hours under the conditions of 25 DEG C
Culture dish is placed under the conditions of different ultraviolet irradiations；
1., in the central location of culture dish, picking colony is inoculated into PDA culture again from ultraviolet processed culture dish
It is small in 25 DEG C of dark conditions on base, it cultivates 48 hours, measures the growing state of bacterium colony；
| Number | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Irradiation time (min) | 15 | 30 | 45 | 60 | 75 | 90 | 105 |
| Average sporulation quantity (a/ml) | 108 | 106 | 106 | 105 | 104 | 104 | 102 |
2., in the central location of culture dish, picking colony is inoculated into healthy growth again from ultraviolet processed culture dish
Tomato leaf on, measure lesion diameter, each processing does three repetitions；
| Irradiation time (min) | 15 | 30 | 45 | 60 | 75 | 90 | 105 |
| Average lesion diameter (mm) | 6 | 4 | 4 | 2 | 2 | 0 | 0 |
2, tomato in plastic greenhouse control method
(1) ultraviolet generation device is set in plastic greenhouse, is killed by control wavelength and irradiation time, the intensity of irradiation
Bacterium；
The ultraviolet generation device are as follows: the UV-B fluorescent tube (240nm-340nm) of 100w；Utilize the cellulose acetate of 0.08mm
Film is coated with away 253mm wavelength components UV-C below；
The radiation intensity of the ultraviolet generation device is 100 μ W/cm2More than, the output energy of ultraviolet lamp is
100kj/m2；
The ultraviolet lamp installation method are as follows: 1.5 meters or so from the ground, be mutually perpendicular to installation ultraviolet lamp, every 20~30 is vertical
Square rice space installs one, and ultraviolet light is radiated at horizontal plane into 60~80 degree of angles；
(2) specific control method
1. the exposure dose 120000uW.s/cm received according to tomato early blight bacterium2—630000W.s/cm2And it is ultraviolet
1000 μ W/cm of generating device output intensity2, exposure dose (W.s/cm2)=irradiation time (s) × intensity (μ W/cm2), it determines
Irradiation time are as follows: 50 minutes；UV intensity will reduce 30%-70% to ultraviolet lamp in use, further according to exposure dose
(W.s/cm2)=irradiation time (s) × intensity (μ W/cm2) determine required irradiation time, when increasing irradiation in practical applications
Between；
2. being irradiated in tomato in plastic greenhouse early blight occurrence injury using ultraviolet lamp, in 30 minutes UV radiation intensities
When reaching stable, start irradiation 45 minutes, it is inhibited to early blight of tomato, Continuous irradiation 5 days；
Example 2, control method of the present invention and common pesticide control compare, and measure control efficiency；
(1) start in the early stages of disease using 75% Bravo wettable powder, 600 times of liquid, 50% thiophanate wettable
600 times of liquid of pulvis, 70% 700 times of Mancozeb liquid, are used alternatingly, and spray 1 time every 10 days, continuous spray 3 times.
(2) the UV-B fluorescent tube (240nm-340nm) of 100w is set in greenhouse；Utilize the cellulose acetate membrane packet of 0.08mm
It is gone out the ultraviolet lamp of 253mm wavelength components UV-C below；1.5 meters or so from the ground, be mutually perpendicular to installation ultraviolet lamp, every 20
~30 cubic metres of spaces install one, and ultraviolet light is radiated at horizontal plane into 60~80 degree of angles；Occur in tomato in plastic greenhouse early blight
When endangering, be irradiated using ultraviolet lamp, when UV radiation intensity reaches stable within 30 minutes, start irradiation 45 minutes, to kind
Eggplant early blight is inhibited, and Continuous irradiation 7 days, irradiation measured disease after 15 minutes, 10 days, 20 days, 30 days daily later
A situation arises.
Claims (1)
1. a kind of method using ultraviolet lamp prevention and treatment tomato in plastic greenhouse early blight, it is characterised in that: occur in tomato in plastic greenhouse early blight
It when endangering, is irradiated using ultraviolet lamp, because ultraviolet lamp is when radiation intensity reaches stable, needs 15 minutes~30 minutes, institute
With ultraviolet light irradiation lethal dose at 45~135 minutes, the daily Continuous irradiation time is not more than 80 minutes, is greater than 80 minutes, shadow
The conduction of signal in plant is rung, the normal vital movement of plant is hindered；So during ultraviolet lamp prevents and treats early blight of tomato,
Irradiation 45-80 minutes daily, it is inhibited to early blight of tomato, Continuous irradiation 5~8 days, germ can be killed；When increase
Between, it is bigger to the inhibiting rate of germ；It irradiates 15~40 minutes, can reduce in greenhouse, disease pest daily when no early blight occurs
Generation；
The UV-B fluorescent tube that the wavelength that the ultraviolet lamp is 100W is 240nm~340nm, utilizes the cellulose acetate membrane of 0.08mm
Coating removes 253mm wavelength components UV-C below；The radiation intensity of the ultraviolet lamp is 100 μ W/cm2More than, ultraviolet lamp
Output energy is 100kJ/m2；
The ultraviolet lamp installation method is 1.5 meters from the ground, is mutually perpendicular to installation ultraviolet lamp, every 20~30 cubic metres of spaces peace
One is filled, ultraviolet light direction of illumination and horizontal plane are at 60~80 degree of angles.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410842162.XA CN105794551B (en) | 2014-12-30 | 2014-12-30 | A method of tomato in plastic greenhouse early blight is prevented and treated using ultraviolet lamp |
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| CN201410842162.XA CN105794551B (en) | 2014-12-30 | 2014-12-30 | A method of tomato in plastic greenhouse early blight is prevented and treated using ultraviolet lamp |
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| CN105794551B true CN105794551B (en) | 2019-07-02 |
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| CN114303821A (en) * | 2020-09-30 | 2022-04-12 | 惠州市四季鲜绿色食品有限公司 | Water and fertilizer integrated planting method for greenhouse peppers |
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Effective date of registration: 20190528 Address after: 745000 225 North Street, Xifeng District, Qingyang, Gansu. Applicant after: QINGYANG DUNBO TECHNOLOGY DEVELOPMENT Co.,Ltd. Address before: 730726 No. 165 Nanshe, Guochengyi Village, Guochengyi Town, Huining County, Baiyin City, Gansu Province Applicant before: Xu Lijiang |
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Effective date of registration: 20230728 Address after: No. 233, Jian'an North Road, Guang'an District, Guang'an, Sichuan Province, 638000 Patentee after: Cainong Technology Group Co.,Ltd. Address before: 745000 225 North Street, Xifeng District, Qingyang, Gansu. Patentee before: QINGYANG DUNBO TECHNOLOGY DEVELOPMENT Co.,Ltd. |
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