CN105420071B - A kind of DNA sequencer with sequencing babinet - Google Patents
A kind of DNA sequencer with sequencing babinet Download PDFInfo
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- CN105420071B CN105420071B CN201510731379.8A CN201510731379A CN105420071B CN 105420071 B CN105420071 B CN 105420071B CN 201510731379 A CN201510731379 A CN 201510731379A CN 105420071 B CN105420071 B CN 105420071B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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Abstract
The present invention relates to a kind of DNA sequencers with sequencing babinet, it includes reaction record subsystem, agent delivery system and control system, and the sequenator babinet of the above-mentioned each system of carrying, wherein, the reaction record subsystem and agent delivery system are separately positioned in left and right two parts section of sequenator babinet；The rear side of reaction record subsystem of the set-up of control system on the left of the babinet in section, control reaction subsystem and agent delivery system are acted according to scheduled sequential；The sequenator babinet is including body supports component, the interior lining panel component being arranged on the inside of body supports component, offside door assembly component, offside door assembly component, and buffer solution box installed unloads platform assembly, left side cover plate assembly, rear side cover plate assembly, upper cover plate.DNA sequencer of the present invention has the body structure of periphery, and sequencing reaction and agent delivery device should have stable, closed operating environment；Overall structure is stablized, and sequencing is efficient.
Description
Technical field
The present invention relates to DNA sequencing fields more particularly to one kind to have body structure, easy to carry, and can independently supply
The DNA sequencer of liquid.
Background technology
In DNA sequencing technology field, pyrosequencing techniques (pyrosequencing), are in 1987 by Nyren et al.
A kind of novel enzyme cascade sequencing technologies to grow up in year, repeatability and accurate performance and Sanger method DNA sequencing skills
Art compares favourably, and speed greatly improves.
Integrated operation flow is described as follows：DNA sample carries out adjunction head, single-stranded catches by after broken using library reagent is built
It obtains, be bound to microballoon, microemulsion PCR amplification, demulsification liquid, obtain and establish DNA library on microballoon, using sample-adding plate by library
The placeds such as the enzyme needed with sequencing reaction to the sequence testing chip with micro reaction pool, install to host by sequence testing chip and sequencing reagent
On, sequencing program is started according to module number and position by control computer, automation carries out sequencing reaction, the data of generation
Data Analysis Computer is transmitted to, computation analysis software carries out image procossing, sequence reading, quality point after completing sequencing
The work such as analysis, sequence assembly finally obtain the sequence information of DNA sample.Micro reaction pool sequence testing chip is the carrier of sequencing reaction,
The DNA Beads and various sequencing reactions for being loaded with sequencing template are respectively positioned on enzyme in the sequence testing chip for being carved with micro reaction pool.
During practical DNA sequencing, it is desirable that sequencing reaction and agent delivery device should have stable, closed behaviour
Make environment；Existing DNA sequencing equipment is difficult to meet the demand.
In view of drawbacks described above, creator of the present invention obtains this creation finally by prolonged research and practice.
Invention content
The purpose of the present invention is to provide a kind of DNA sequencer with sequencing babinet, to overcome above-mentioned technological deficiency.
To achieve the above object, the present invention provides a kind of DNA sequencer with sequencing babinet, including reaction record point
The sequenator babinet of system, agent delivery system and control system and the above-mentioned each system of carrying, wherein, reaction note
Record subsystem and agent delivery system are separately positioned in left and right two parts section of sequenator babinet, and by being arranged on babinet
Interior agent delivery pipeline connection；After reaction record subsystem of the set-up of control system on the left of the babinet in section
Side, control reaction record subsystem and agent delivery system are acted according to scheduled sequential；
The sequenator babinet includes body supports component, the interior lining panel component being arranged on the inside of body supports component,
Offside door assembly component, offside door assembly component, buffer solution box installed unload platform assembly, left side cover plate assembly, rear side cover plate assembly,
Upper cover plate；
Babinet is divided into left and right two parts, reaction record subsystem is provided in the babinet of left side, including reaction warehouse component and CCD
Camera；Agent delivery system is provided in right case, including pump valve pipe integrated unit, liquid case hoistable platform and display,
Control assembly is provided on rear side of babinet, is adjusted to the temperature for detecting reaction tank and according to the threshold value of setting；
Reagent delivery to reacting in record subsystem, is reacted record point by the control system control agent delivery system
Reaction warehouse component is provided in system, reagent flows through the reaction chip being arranged in reaction warehouse component and reacts, reaction record
CCD camera in subsystem acquires the photo in reaction process and obtains surveyed DNA sequence dna after analysis.
Further, the bottom of the body supports modular support babinet, side, rear portion and left and right Liang Ge babinets area
The cutting part in domain；
Body supports component is made of steel plate splicing, is fixed by way of splicing or screw fastening；It includes pedestal
Component, top plate member and base assembly connect and compose the support frame assembly of babinet side frame, buffer system mounting base；
Wherein, the support frame assembly include left side vertical plate component, separate left and right two tanks intermediate riser component,
Right side riser component and be arranged on right side riser forward portion right side mend slab, rear side riser component, be arranged on top plate group
The first cable fixed seat and the second cable fixed seat on part.
Further, reaction record subsystem includes reaction warehouse component, motion platform component, motion control list
Member, CCD camera and platform support component, wherein, reaction chip component is arranged inside in the reaction warehouse component, is in control
Under the action of system, reaction solution, which enters in reaction chip, to be chemically reacted, and generates visible ray；
CCD camera, is fed into appropriate picture-taking position by the motion platform component, so that CCD camera is to reaction
The visible optical information generated in chip carries out acquisition of taking pictures；The platform component, the support reaction warehouse component,
Motion platform component；The motion control unit controls the operation of CCD camera and reaction chip in real time, driving fortune
Moving platform component movement detects and controls the temperature of reaction warehouse to the picture-taking position of CCD camera.
Further, the left side vertical plate component, intermediate riser component surround the reaction capacity for accommodating reaction warehouse component
Put chamber；First cable fixed seat is set in top plate member, which is divided into left side babinet the reaction warehouse accommodating cavity of front side
With the circuit board accommodating cavity of rear side；Intermediate riser component and right side riser component surround reagent chamber.
Further, the top plate member, including front side board, right plate, back side panel, left plate, above-mentioned each plate it
Between be sequentially connected with successively, and each plate is bending plate, including vertical plate and level board, vertical plate is arranged on the outer of level board
Side, the front and rear connection of level board form support section；
Intermediate plate is connected between front side board and back side panel, isolation board is provided between intermediate plate and left plate；
Fluid path accommodating cavity is formed between intermediate plate and right plate, reaction warehouse accommodating cavity is formed between front side board and isolation board.
Further, further include left side storehouse trim panel assembly component, include being arranged on the first trim panel of centre and
The first trim panel both sides are separately positioned on and the second trim panel connected to it and third trim panel, in the outside of the second trim panel
It is also associated with the first interior trim and mends slab and the second interior trim benefit slab.
Further, the reaction warehouse component includes the vertical door frame being arranged on the platform component,
Turnable reaction warehouse component is provided on the top in the outside of door frame, is connected in the lower end that reaction warehouse component may be reversed turnable
Pedestal, turnable basement are fixed on the middle part of door frame, and reaction warehouse component, which may be reversed, to be rotated around the turnable basement, to
Take out or be put into reaction chip；
The turnable reaction warehouse component includes warehouse may be reversed, and reaction chip is provided on the inside of warehouse may be reversed
Component, is equipped with reaction chip in reaction chip component, and reagent solution is chemically reacted by reaction chip.
Beneficial effects of the present invention are compared with prior art：DNA sequencer of the present invention has the body structure of periphery, surveys
Sequence is reacted and agent delivery device should have stable, closed operating environment；Overall structure is stablized, and sequencing is efficient.Sequenator
There should be continuous, controllable characteristic during agent delivery, sequencing reaction.
The DNA sequencer of the present invention has the robust structure of multiple adjustment, easy for installation between each structure, compact-sized,
Stability is high.Reaction warehouse component can be installed, be fixed and adjust the reaction chip, ensure reaction chip in stable environment
In reacted, also, reaction chip can facilitate taking-up and locking.
The present invention is in the reaction warehouse component of DNA sequencing and the periphery setting babinet of reagent component so that reaction warehouse has
Enough precision, stability and clean spaces；Space is reasonable, is easily portable adjustment.
Babinet is provided with left and right two parts space, accommodates reaction component and agent delivery, storage assembly, left and right space respectively
Interior equipment works respectively, forms streamlined operation, and testing efficiency is high.
The liquid-supplying system of the DNA sequencer of the present invention controls storage, conveying, the selection of reagent by control unit；Liquid case
Hoistable platform component is moved by guide rail by reagent delivery to designated position, easy to control, accurate；Liquid case hoistable platform is by pump valve
Integrated tube assembly ease up fliud flushing box installed unload platform assembly up and down transport, complete reagent conveying, reaction process.
Description of the drawings
Fig. 1 is the functional block diagram of the DNA sequencer of the present invention；
Fig. 2 a are the overall structure diagram of the DNA sequencer babinet of the present invention；
Fig. 2 b are the first structure schematic diagram of the body supports component of the DNA sequencer babinet of the present invention；
Fig. 2 c are the second structure diagram of the body supports component of the DNA sequencer babinet of the present invention；
Fig. 3 is the overall structure diagram of the DNA sequencer reaction record subsystem of the present invention；
Fig. 4 is the first dimensional structure diagram of the reaction warehouse component of the present invention；
Fig. 5 a are the first dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 5 b are the second dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 6 a are the dimensional structure diagram of the locating piece of the present invention；
Fig. 6 b are the structure diagram of the electronic lock of the present invention；
Fig. 6 c are the structure diagram of the electronics lock of the present invention；
Fig. 6 d are the schematic cross-sectional view of the electronic lock of the present invention；
Fig. 7 is schematic cross-sectional view of the turnable reaction warehouse component of the present invention along first lug；
Fig. 8 is the third dimensional structure diagram of the turnable warehouse of the present invention；
Fig. 9 a are the structure diagram of the reaction chip component of the present invention；
Fig. 9 b are the structure diagram of the reaction chip mounting base of the present invention；
Fig. 9 c are the structure diagram of the medial surface of the reaction warehouse pedestal of the present invention；
Fig. 9 d are the structure diagram of the lateral surface of the reaction warehouse pedestal of the present invention；
Fig. 9 e are the structure diagram of the temperature control component cover board of the present invention；
Figure 10 a are the schematic cross-sectional view along liquid inlet and outlet of the reaction chip component of the present invention；
Figure 10 b are schematic cross-sectional view of the turnable reaction warehouse component of the present invention along adaptive adjustment part；
Figure 11 is the turnable reaction warehouse component schematic cross-sectional view of the present invention；
Figure 12 a are the schematic cross-sectional view of the back shroud of the present invention；
Figure 12 b are the dimensional structure diagram of the back shroud component of the present invention；
Figure 12 c are the dimensional structure diagram of the back shroud of the present invention；
Figure 13 a are the dimensional structure diagram of the top plate member of the DNA sequencer babinet of the present invention；
Figure 13 b are the dimensional structure diagram of the left side storehouse trim panel assembly component of the DNA sequencer babinet of the present invention；
Figure 14 a are the dimensional structure diagram of the liquid case hoistable platform component of the present invention；
Figure 14 b are the side structure schematic view of the liquid case hoistable platform component of the present invention；
Figure 15 a are that the pump valve of the present invention integrates the dimensional structure diagram of tube assembly；
Figure 15 b are that the pump valve of the present invention integrates the overlooking the structure diagram of tube assembly；
Figure 16 a are that the buffer solution box installed of the present invention unloads the dimensional structure diagram of platform assembly；
Figure 16 b are that the buffer solution box installed of the present invention unloads the positive structure diagram of platform assembly.
Specific embodiment
Below in conjunction with attached drawing, the forgoing and additional technical features and advantages are described in more detail.
Refering to Figure 1, the functional block diagram of its DNA sequencer babinet for the present invention, the DNA of the embodiment of the present invention is surveyed
Sequence instrument includes reaction record subsystem, agent delivery system and the sequenator babinet of control system and the above-mentioned each system of carrying,
In the present embodiment, the reaction record subsystem and agent delivery system are separately positioned on the left and right sides of sequenator babinet
In section, and the agent delivery pipeline by being arranged in babinet connects；Set-up of control system section on the left of babinet
The rear side of interior reaction record subsystem, control reaction record subsystem and agent delivery system are acted according to scheduled sequential.
Reagent delivery to reacting in record subsystem, is reacted record point by the control system control agent delivery system
Reaction warehouse component is provided in system, reagent flows through the reaction chip being arranged in reaction warehouse component and reacts, reaction record
CCD camera in subsystem acquires the photo in reaction process and obtains surveyed DNA sequence dna after analysis.
It please refers to shown in Fig. 2 a, the overall structure diagram of the DNA sequencer babinet for the present invention, the embodiment of the present invention
Sequenator babinet to include body supports component 21, the interior lining panel component for being arranged on the inside of body supports component 21, offside door total
Platform assembly 25, left side cover plate assembly 30, rear side cover board group are unloaded into component 23, offside door assembly component 26, buffer solution box installed
Part, upper cover plate 3110.
It is right that the interior lining panel component includes left side storehouse interior finishing panel assembly 24, right side storehouse left side interior finishing panel assembly and right side storehouse
Side interior finishing panel assembly 22.
In embodiments of the present invention, babinet is divided into left and right two parts, reaction record subsystem is provided in the babinet of left side, i.e.,
Reaction warehouse component and CCD camera；Agent delivery system is provided in right case, is lifted including pump valve pipe integrated unit, liquid case
Platform and display, are provided with control assembly on rear side of babinet.At work, pump valve integrated unit by reagent solution according to suitable
Sequence is delivered in reaction warehouse component, completes chemical reaction, and under the control of the control unit, CCD camera completion is taken pictures, and finally pass through
Data analysis is crossed to take pictures as a result, point drawing a conclusion.
Wherein, the segmentation of the bottom of 21 supporting box of body supports component, two side, rear portion and left and right housing areas
Portion；Plaque is provided on the medial support component of two housing areas in left and right, is played heat-insulated and increase structural stability
Effect；In left side, the forepart of babinet is provided with offside door assembly component 23, can be to being arranged on when opening offside door assembly component
Reaction warehouse component in left box body is operated；It is provided with offside door assembly component 26 on the right side of the babinet of right side, it, can during opening
Pump valve integrated unit is operated, various reagents pipe and control valve road are set in the subregion.
It please refers to shown in Fig. 2 b-2c, the structure diagram of the body supports component for the present invention；The embodiment of the present invention
Body supports component is made of steel plate splicing, is fixed by way of splicing or screw fastening；It include base assembly 212,
Top plate member 2112 connects and composes the support frame assembly 211 of babinet side frame, buffer system installation with base assembly 212
Seat 213.Wherein, the support frame assembly 211 includes left side vertical plate component 2116, the centre of separation left and right two tanks is stood
Plate component 2115, right side riser component 2117 and be arranged on right side riser forward portion right side mend slab 2119, side stand rearways
Plate component 2118, the first cable fixed seat 2113 being arranged in top plate member 2112 and the second cable fixed seat 2114,
And the connecting plate 2133 that each component links together.
Wherein, the left side vertical plate component 2116, intermediate riser component 2115 surround the reaction for accommodating reaction warehouse component
Storehouse accommodating cavity 200；Second cable fixed seat 2114 is set in top plate member, which is divided into the anti-of front side by left side babinet
Answer the circuit board accommodating cavity 20 of storehouse accommodating cavity 200 and rear side；Intermediate riser component 2115 and right side riser component 2117 surround examination
Agent chamber 2001.The middle steel plate of top plate member is provided with the first bending plate 2141 and the second bending plate 2142, the two one into
The part that the upward bending of type, respectively steel plate both sides is formed, forms between the first bending plate 2141 and the second bending plate 2142
Wire casing passes through for conducting wire.
The buffer system mounting base 213 includes being separately positioned on left side vertical plate component 2116 and intermediate riser component
Platform support panel 2132 and Reagent Tube support base 2131 on 2115 set platform group on the platform support panel 2132
Part and reaction warehouse component thereon.
Babinet of the present invention is connected using plate, not only easy for installation, and the structural strength that various components link together
Height, can support the weight of reaction warehouse component, camera and pump valve integrated unit, and is capable of providing and stablizes, reliable environment.
It please refers to shown in Fig. 3, the overall structure diagram of the DNA sequencer reaction record subsystem for the present invention, this
In inventive embodiments reaction record subsystem include reaction warehouse component 12, motion platform component 14, motion control unit 15,
CCD camera 101 and platform support component 13, wherein,
The reaction warehouse component 12, is arranged inside reaction chip component, and under the action of control system, reaction solution enters
It is chemically reacted in reaction chip, generates visible ray；The motion platform component 14, CCD camera is fed into suitably
Picture-taking position, acquisition of taking pictures is carried out to the visible optical information generated in reaction chip；The platform component 13, branch
The support reaction warehouse component 12, motion platform component 14；The motion control unit, to CCD camera and reaction core
The operation of piece controls in real time.
In embodiments of the present invention, the reaction warehouse component 12 can be installed, be fixed and adjust the reaction chip,
Ensure that reaction chip is reacted, also, reaction chip can facilitate taking-up and locking in stable environment.
It please refers to shown in Fig. 4, the reaction warehouse component 12 includes being arranged on perpendicular on the platform component 13
The door frame 121 directly set is provided with turnable reaction warehouse component 124 on the top in the outside of door frame 121, in turnable reaction warehouse
The lower end of component 124 is connected with turnable basement 122, and turnable basement 122 is fixed on the middle part of door frame 121, and reaction may be reversed
Storehouse component 124 can be rotated around the turnable basement 122, to take out or be put into reaction chip.
Electronics lock set 125 is additionally provided on the left of the upper end of the door frame 121, only in electronics lock set 125
During opening, turnable reaction warehouse component 124 can be rotated, reaction chip is operated.In embodiments of the present invention, if
Door frame 121 is equipped with, supports reaction chip and dependency structure, substantially increases the stability of system.
Reaction warehouse component 124 may be reversed to include warehouse may be reversed, be equipped in the middle section of the turnable warehouse
Back shroud 1242, to encapsulate Reagent Tube；The first rotating part is provided in lower part, including the first accommodating chamber 1243, to hold
Receive turnable basement 122, the first accommodating chamber 1243 is arranged on the centre position of lower part；It is arranged symmetrically in the first accommodating chamber both sides
First lug 1244, the centre of first lug 1244 is provided with through-hole 1246；To connect with the turnable basement 122
It connects.Turnable basement is rotated relative to turnable warehouse in the first accommodating chamber 1243, is had higher stability, is ensured simultaneously
The transmission of reagent and data is unimpeded.Door frame 121 has the structure of side baffle 1211 for one side, is integrally formed.
It please refers to shown in Fig. 4, there are two the companies of the second lug 1221 for 122 tools of turnable basement in the embodiment of the present invention
Pedestal is connect, the second lug 1221 is symmetricly set on a side 1223 of pedestal, is provided with through hole 1224 among it, with institute
The intermediate through-hole 1246 set for the first lug 1244 on turnable warehouse stated is co-axially mounted, also, the second lug 1221
Mounted on the inside of first lug 1244.
Incorporated by reference to shown in Fig. 4, in embodiments of the present invention, the both sides of the turnable basement 122 are arranged on side gear
Positioning is contacted in plate 1211 and with side baffle 1211；The first lug 1224 is co-axially mounted with the second lug 1221
Afterwards, end and the upper-end contact of turnable basement 122, also, the side of warehouse and the side baffle of door frame 121 may be reversed
1211 medial surfaces contact, and ensure that warehouse, which may be reversed, to be located just at turnable basement 122 in side baffle 1211.
It please refers to shown in Fig. 5 a and 5b, reaction chip component is installed on the inside of the centre of the turnable warehouse,
Correspondingly, the first cavity 1785 for accommodating reagent pipeline is provided in the centre that warehouse may be reversed, at the bottom of the first cavity 1785
Portion is provided with first boss 1784, and first boss 1784 is convex first to carry the support element of the reaction chip component
For the setting of platform 1784 there are four guide rod mounting hole 1786, guide rod mounting hole 1786 passes through the both ends that warehouse may be reversed, reaction core
Reaction chip component is connect by piece component and support element with turnable warehouse；It is set in the upper end of the first boss 1784
It is equipped with second boss 1783, second boss 1784 is positioning the support element；It is set in 1784 upper end of second boss
Positioning surface 1782 is equipped with, locking screw nail hole 1787 is provided on positioning surface 1782.Described in above-mentioned first cavity body structure positioning
Reaction chip component, also, reaction chip component is in normal work, it is impossible to and it rotates, is merely able to move along guide rod.
1788 He of Reagent Tube mounting hole is provided on the upper surface of the first accommodating chamber 1243 of the turnable warehouse
Electric wire mounting hole is equipped with pipe clamp 03 in Reagent Tube mounting hole 1788, and being passed through in pipe clamp 03 has Reagent Tube 02 and electric wire 01.
The lock tongue installation of the lock tongue 1252 in electronics lock set 125 is additionally provided in the side of the turnable warehouse
Hole；Under the action of locking member, warehouse may be reversed and be fixed together with door frame 121.
The upper end in the outside of the turnable warehouse is provided with pressing piece 1241, is only pressed by pressing piece 1241
Pressure setting can rotate the turnable warehouse.In embodiments of the present invention, incorporated by reference to shown in Fig. 8, the pressing
1241 one end of part are provided with opening 1730, one end is provided with the structure of hook, and the upper end-stopping plate of the hook and door frame connects
It connects, when pressing piece 1241 is by downward active force, hook is detached from door frame baffle, and reaction warehouse body may be reversed can be with respect to door
Frame rotates.
In embodiments of the present invention, the pressing piece 1241 is mounted in a positioning block 1762, under locating piece 1762
End is provided with spring 1761, and spring is arranged in spring base 1763, and spring base 1763, which is threadably mounted at, may be reversed reaction warehouse
In the spring base hole 1781 of 1785 upper surface of intermediate throughholes.
Incorporated by reference to shown in Fig. 5 b-5d, locating piece 1762 is mounted on the locating piece installation cavity that reaction warehouse upper end may be reversed
In 1791, it is provided with positioning step 1790 in the cavity.Locating piece 1762 in the embodiment of the present invention includes upper 1794 He of through-hole
Lower through-hole 1797, one of both pass through the pressing piece 1241；The side of locating piece set processing location hole 1796 and
Mounting hole 1795.The lower end of the through-hole of installation pressing piece 1241 is provided with spring eye, spring passes through the hole and pressing piece 1241
Bottom contact.
The embodiment of the present invention sets electronics lock set and pressing piece, and two are carried out to reaction warehouse component and door frame may be reversed
Automatically controlled and mechanical structure mode is respectively adopted in secondary locking, ensures that reaction chip is run in security context, avoids maloperation.
Incorporated by reference to shown in Fig. 6 b and 6c, electronics lock set 125 of the invention includes electronics lock and inductive switch 1251, institute
The inductive switch stated is mounted on electronics lock, and electronics lock is arranged on the side of turnable warehouse, in embodiments of the present invention,
Electronics lock includes side installing plate 1843, and mounting hole 1844 is offered on side installing plate 1843；Electronic lock work passes through peace
Dress hole 1844 and mounting screw are fixed on turnable warehouse.It is provided with symmetrically in 1843 side of side installing plate
First framework 1841 and second framework 1842, in the centre of the first framework and second framework, there are installation inductive switches 1251
Space.
In embodiments of the present invention, the inductive switch 1251 includes being arranged on external framework 1255, framework 1255
One side on be provided with connection screw thread hole 1256, fixed with the electronics lock by attachment screw 1254；Described
Attachment screw 1254 is by 1258 and first framework 1841 of lug, the second framework 1842 on side, to increase electronics lock
Bonding strength.Control circuit 1252 is also associated on the inductive switch 1251, to be carried out to inductive switch 1251
Control.
It please refers to shown in Fig. 6 d, the electronics lock set 125 is mounted on the side that warehouse and door frame 121 may be reversed, lock
Tongue 1253 enters after stretching out to be arranged in the dead bolt hole 1247 of turnable warehouse side, realizes locking；When lock tongue 1253 is pierced in electricity
Under the action of swashing, when retracting from dead bolt hole 1247 is interior, the turnable warehouse can be rotated.
The use of door frame, be not only able to so that reaction chip component stable structure, moreover, also so that may be reversed warehouse with
Turnable basement is compact-sized, easy to operation；The positioning of CCD camera and reaction chip is more accurate.
The first mounting hole 1210 is provided in the lower part of door frame 121, to connect fixation with motion platform component 14；In door
The second mounting hole being connect with turnable basement side is additionally provided in the middle part of frame.
Incorporated by reference to shown in Fig. 7, in embodiments of the present invention, in order to save structure space and ensure the steady of reagent delivery
It is fixed, it is provided with examination in the through hole of intermediate 1246 and second lug 1221 of through-hole set of the first lug 1244
The first fixation kit of agent pipe, including the pipe joint 1265, the first washer 1264, the first rotary shaft being arranged in the lug of left side
1261 and be arranged on right side lug in reagent pipe fitting 1266, second packing ring 1267, the second rotary shaft 1269, wherein, institute
The first washer 1264 and second packing ring 1267 stated are arranged between turnable warehouse and turnable basement, in the present embodiment,
The diameter of the intermediate through-hole 1246 set of the first lug 1244 is less than the diameter of the through hole of the second lug 1221,
The positioning rib positioned to washer is formed in the medial end of the two, after two washers are installed, inside diameter of gasket is just with
The diameter of the through hole of two lugs 1221 is identical.Setting positioning rib and washer in embodiments of the present invention ensure may be reversed
Gapless between warehouse and turnable basement, the stability of the two after combining is good, will not be shaken because of there are gap, and
And washer increases the contact area of the two so that stress between the two reduces, and extends service life of equipment.
The first rotation is installed on the inside of the inside of the intermediate through-hole 1246 set of first lug 1244 and washer
1261 and second rotary shaft 1269 of axis, the structure of two rotary shafts is identical, is the hollow shaft with positioning convex ring；In rotary shaft
One end setting positioning convex ring, connection screw thread hole is provided with, to the lug connection portion with the turnable warehouse
1263 connections are fixed, correspondingly, the end set in the through-hole 1246 has the positioning recess that positions the positioning convex ring.
The centre at the both ends of rotary shaft 1269 be recess, inside recess to the pipe joint 1265, reagent pipe fitting
1266 installation positioning.Electric wire and Reagent Tube 02 are separately installed in pipe joint 1265, reagent pipe fitting 1266, is distinguished
Lead in reaction chip component.Due to setting the component that can mutually overturn in the embodiment of the present invention, in overturning, to avoid to pipe
Part impacts, and installs Reagent Tube in through-holes, substantially increases the security performance of Reagent Tube.
It please refers to shown in Fig. 8, in embodiments of the present invention, the reaction chip component is mounted on the turnable storehouse
The inside of body.The observation portion 1724 contacted with CCD camera is provided on the inside of reaction chip component, is provided with transparent
Baffle, to take pictures to reaction process.
It please refers to shown in Fig. 9 a, reaction chip component of the invention includes reaction chip mounting base 127, reaction warehouse pedestal
Component 126 and the shading positioning component 128 that reaction warehouse base assembly 126 and reaction chip mounting base 127 are locked, opened；
In order to easily take out and be closed to reaction chip, reaction chip mounting base 127 can be with 126 phase of reaction warehouse base assembly
To rotation.
It please refers to shown in Fig. 9 b, the structure diagram of the reaction chip mounting base for the present invention is installed in reaction chip
The middle section of seat 127 is equipped with reaction chip, and cover board 1277 is provided on reaction chip；It is installed in the both sides of reaction chip
There is sliding slot pressing plate 1279, reaction chip to be fixed.The side edge of reaction chip mounting base 127 is provided with installation
Seat supports component, in taking-up or chip placement, to support the reaction chip mounting base；It includes torsional-spring supported portion
1273, it is arranged in mounting base, to support the torsional spring, the torsional-spring supported portion 1273 carries protruding end 1274 for one
Recess, in the rotation reaction chip mounting base 127, protruding end 1274 contacts at first with 1261, in the present embodiment,
The protruding end 1274 is arranged on the intermediate position at recess and the edge of chip installation position；Using the structure of mounting base in itself
Reaction chip mounting base is supported, better reliability, ensures that chip can be safely removed and be placed.
The drawing that the mounting base support component further includes torsional spring 1272, connect with torsional spring 1272 and be arranged on torsional spring both ends
Mandrel 1271, the tension axis 1271 is mounted on lug boss 1275, when starting reaction chip mounting base, the torsion
Spring 1272 supports mounting base, and finally relies on the positioning of protruding end 1274, realizes support.
The first rotary shaft hole 1276 is additionally provided in the end of the lug boss 1275, to reaction warehouse pedestal 1261
Connection.Locating slot 1278 is additionally provided in the side of reaction chip mounting base.
It please refers to shown in Fig. 9 c, the structure diagram of the medial surface of the reaction warehouse pedestal for the present invention, in reaction warehouse
Reaction pond body 1266 is provided in medial surface, reaction tank 1267 is set in 1266 middle section of reaction pond body, in reaction tank 1267
Surrounding 1706 be provided with sealing ring, prevent reaction reagent from leaking.One end of reaction warehouse pedestal 1261 is provided with raised line 1705,
To be positioned with the cover board after installation.The other end be provided with the corresponding torsional-spring supported structure of reaction chip mounting base,
Including symmetrically arranged two protruding ends 1268, it is provided with the second rotary shaft hole 1269 on it, with the first rotary shaft hole
1276 is coaxial, and rotary shaft sequentially passes through holes；The region 1260 and localization region for torsional spring activity are additionally provided in the end
1707, the threaded hole 1712 reaction Hatch Board will be installed is provided in the region.
It please refers to shown in Fig. 9 d, the structure diagram of the lateral surface of the reaction warehouse pedestal for the present invention, at reaction warehouse bottom
The middle section of the lateral surface of seat is provided with temperature control component, and temperature sensor installation is provided in the middle section of reaction warehouse pedestal
Slot 1262 is provided with heating wire mounting groove 1704 in the bottom of mounting groove 1262, places heating wire and temperature sensor respectively.
A positioning convex platform 1703 is additionally provided in mounting groove, to be positioned when being installed with temperature control component cover board 1263.In temperature control group
It is provided on part cover board 1263 and 1703 corresponding positioning recess 1702 of positioning convex platform.
It sets there are four guide rod mounting hole 1742 in the surrounding of temperature sensor mounting groove 1262, is set in upper and lower sides
There is adaptive spring mounting hole 1741；It is also equipped with being oriented to rod aperture 1701 on cover board 1263.
Shading positioning component mounting groove 1265 is additionally provided on reaction warehouse pedestal, to install the shading positioning group
Installation base 1744 is being arranged inside in part 128.
Incorporated by reference to shown in Figure 10 a-10b, the intermediate position of reaction warehouse pedestal is provided with electric heating wire 1754, is arranged on
In the heating wire mounting groove 1704 of pedestal；Temperature sensing is provided in the temperature sensor mounting groove 1262 on electric heating wire top
Device 1720 and control assembly are adjusted to the temperature for detecting reaction tank and according to the threshold value of setting；In temperature sensor
1720 and control assembly 1753 also pour and be inoculated with sealing element 1263, be encapsulating resin.Examination is respectively arranged at the both ends of reaction tank
Agent pipe installs threaded hole 1721, to be connect with Reagent Tube；The end of Reagent Tube installation threaded hole 1721 is provided with inlet
1723 and liquid outlet 1713, liquid inlet and outlet communicated with the both ends of reaction tank, to cycle detection reagent.
The avris of the interior grooves of reaction tank is provided with sealing ring 1713.It is equipped in the centre of reaction chip mounting base
Reaction chip 04, reagent flow through reaction chip and react.
In embodiments of the present invention, by the Reagent Tube mounting hole 1788 of turnable warehouse, being arranged on can turn over Reagent Tube 02
The centre of roll-over body is provided with the first cavity 1785 for accommodating reagent pipeline, and final coupled reaction pond body, reagent cartridge is by each
The sealing of kind cavity, stability is high, is hardly damaged.
Back shroud 1242 is fixed on rear side of reaction warehouse, the centre of back shroud 1242 is provided with adaptive adjustment part
1751, it please refers to Fig.1 shown in 2a-12c, two rows of installation raised lines 1809 is provided among the inside of back shroud 1242, are being installed
The centre of raised line 1809 is provided with the first installation base 1812 and the second installation base 1811, and screw thread is offered on installation base
Hole 1802, adaptive adjustment part 1751 are mounted in the threaded hole 1802.
In embodiments of the present invention, the connecting pin of the adaptive adjustment part 1751 and back shroud is the end of thread, and anti-
The connecting pin for answering storehouse component is the polished rod 1805 for being provided with spring 1806, is provided with nut 1804 between the ends and is positioned,
After installation is complete, nut 1804 is close to installation base.
It is channel 1803 between two raised lines 1809, Reagent Tube is enabled to pass through；It is also set on back shroud
It is equipped with the stiffener plate 1812 vertical with raised line 1809；In raised line 1809 and 1810 bit plane of upper surface of boss；In plate below
Peripheral framework 1807 centre and quadrangle be provided with trip bolt hole 1801, to connect with the described turnable warehouse that reacts
Connect fixation.
Above-mentioned back shroud component is not only able to carry out face sealing to reaction warehouse component, additionally it is possible to the fixed adaptive tune of installation
One integral piece, it is simple in structure.
In embodiments of the present invention, it may be reversed between reaction warehouse and reaction warehouse pedestal 126 and be provided with adaptive adjustment part
1751, the end spring of adaptive adjustment part 1751 is contacted with the end face of reaction warehouse pedestal；When CCD camera is used to feed, phase
When machine is with reaction chip component touch, when adjusting camera position, the mode that back-and-forth motion may be used adjusts, at this point, adaptive
It answers adjustment part that can change the position of reaction chip component according to the change in location of camera, ensures that there is higher accuracy of detection,
Meanwhile it avoids damaging system.
Please refer to Fig.1 shown in 3a-13b, top plate member 2112 in embodiments of the present invention, including front side board 2124,
Right plate 2125, back side panel 2143, left plate 2153 are sequentially connected with, and each plate is bending successively between above-mentioned each plate
Plate, including vertical plate and level board, vertical plate is arranged on the outside of level board, and the front and rear connection of level board forms support section.
Intermediate plate 2123 is connected between the 2143 of front side board 2124 and back side panel, in intermediate plate 2123 and left plate 2153
Between be provided with isolation board 2128；Fluid path accommodating cavity is formed between intermediate plate 2123 and right plate 2143, in front side board 2124
Reaction warehouse accommodating cavity is formed between isolation board 2128.
In embodiments of the present invention, the upper end connection upper cover plate 3110 of top plate member 2112, in the vertical of right plate 2125
Portion is provided with integrally formed connection sheet 2151；The vertical portion of back side panel 2143 is provided with connecting plate 2133.
The wire casing that is made of its bending part is set on intermediate plate 2123, and including rear side wire casing 2127, conducting wire is from rear side line
Slot 2127 is passed through by the second cable fixed seat 2114 in reaction warehouse component.It is front side connecting portion 2122 in front side, left side is
Left side connecting portion 2121, is threaded hole thereon, to be connect with upper cover plate；Left side is the first rear side connecting portion 2130, rear side
For the second rear side connecting portion 2129；Setting lock 2150 on second rear side connecting portion 2129.
The edge of top plate is provided with connecting portion 2152.
The left side storehouse trim panel assembly component of the present invention, including being arranged on the first intermediate trim panel 241 and difference
241 both sides of the first trim panel are arranged on and the second trim panel 242 connected to it and third trim panel 243, in the second trim panel
242 outside is also associated with the first interior trim and mends 244 and second interior trim of slab benefit slab 245.
The agent delivery system of the embodiment of the present invention includes control unit, and buffer solution box installed unloads platform assembly, liquid case lifting
Platform assembly, pump valve integrate tube assembly, wherein, the buffer solution case loading/unloading platform can load and unload various reagents, and by liquid
The upper and lower of case hoistable platform component is transported to the position specified, and for the pump valve integrated pipe Component driver, the pump valve integrates
The Reagent Tube that tube assembly selection needs, the open and close control of driving and hydraulic valve through hydraulic pump, by specified reagent delivery extremely
It is reacted in reaction warehouse component；The action of above-mentioned each element is uniformly controlled by control unit.
The liquid case hoistable platform component of the embodiment of the present invention is the mechanism that pumps of motor leading screw driving, is installed
In the right side babinet of sequenator.Its support base for including driving motor 401, being connect with driving motor and supporting driving motor
402nd, lead screw assembly, the reaction liquid case lifting seat 408 mounted on side being connect with driving motor, wherein, the reaction
Platform assembly is unloaded with buffer solution box installed is installed in liquid case lifting seat 408, reaction is mounted on liquid case lifting seat 408 with reacting
On installing plate 404 with the connection of liquid case lifting seat, under the driving of screw mechanism, reaction liquid case lifting seat Reagent Tube group is carried
It moves up and down.
The intermediate plate 4081 that reaction includes connecting with installing plate with liquid case lifting seat 408 is arranged on the support of intermediate plate both sides
Plate 4082 is provided with horizontal ends on supporting plate 4082, platform assembly is unloaded to place buffer solution box installed.
The output shaft of the driving motor 401 is connect with leading screw 403, and leading screw is arranged in pedestal 405, pedestal 405 with
The end of driving motor 401 is connected by support base 402；Stroke detection switch 406 is provided in the side of pedestal 405.It is described
The output shaft of driving motor connect by shaft coupling 4035 with the connecting pin 4034 of leading screw；Nut 4031 is connected on leading screw,
Nut is fixed on nut seat 4032；By bearings, bearing end is provided with bearing (ball) cover 4011 for leading screw lower end.
The liquid case hoistable platform component of the embodiment of the present invention is moved by guide rail by reagent delivery to designated position, controlling party
Just, precisely；Liquid case hoistable platform by pump valve integrate tube assembly ease up fliud flushing box installed unload platform assembly up and down transport, complete reagent it is defeated
It send, reaction process.
The pump valve of the embodiment of the present invention integrates tube assembly and is unloaded reagent from buffer solution box installed by the syringe needle suction pipe 507 of downside
It is sucked in Reagent Tube on platform assembly, and passes through the selection of fluid path control unit 502, drive control valve module 509 and hydraulic pressure
Pump 506 is selected, and reagent is discharged into reagent cartridge, carries out sequencing reaction.
The pump valve integrates tube assembly and includes pump valve integrated base plate 508, and pump valve integrated base plate 508 includes peristaltic pump
Installing plate 5011, the fixed plate being connect with peristaltic pump installing plate 5011, bottom plate, are arranged on sipper pore group and bottom on bottom plate
Control valve group installing plate among plate；Peristaltic pump mounting hole is provided on peristaltic pump installing plate 5011, sets two thereon
Peristaltic pump 505；Multiple reagent pipe clamps and wire clamp 5015 are also arranged at intervals on bottom plate.Correspondingly, control valve group 509 is pacified
Mounted in the middle section of pump valve integrated base plate 508, hydraulic pump 506 is mounted on its side, and in the other side, setting fluid path controls
Unit 502, fluid path control unit 502 are isolated by a bending plate 503 with fluid path part.
In embodiments of the present invention, in order to carry out sequencing reaction to plurality of reagents, the sipper pore group on bottom plate is side by side
Multiple holes are provided with, syringe needle suction pipe 507 passes through sipper pore, and the other end connects Reagent Tube and connects with the fluid path port of corresponding control valve
It connects；Pipe clamp 5013 on sipper pore is set, prevents syringe needle suction pipe from tilting.
The embodiment of the present invention integrates tube assembly using pump valve, selection can be carried out at the same time to plurality of reagents, and according to pre-
The sequence first set carries out sequencing reaction, and testing efficiency is high.
Buffer solution box installed unloads the structure that platform assembly is drawing and pulling type, after setting reagent on platform, entire platform assembly peace
In the babinet of right side, when needing to load and unload reagent, platform is pulled out, after placing, pushed home is put again.
It includes carrier fluid platform 310, in the present embodiment, the pulling plate of knob 3101 is carried, and shake hands to fix for one
On handle plate；The both sides of carrier fluid platform 310 are equipped with connecting plate 311, and the horizontal part of connecting plate 311 is with reacting with liquid case liter
The supporting plate connection of seat 408 is dropped；Under the action of reaction liquid case lifting seat, carrier fluid platform 310 moves up to pump valve integrated pipe
At the syringe needle suction pipe 507 of downside, reagent is selectively drawn in the control through control unit.
Slide track component 312 between the carrier fluid platform 310 and the connecting plate 311 of both sides is set, makes carrier fluid platform
It is moved along connecting plate.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, the embodiment not to
The scope of the claims of the present invention is limited, all equivalence enforcements or change without departing from carried out by the present invention are intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (6)
1. a kind of DNA sequenators with sequencing babinet, which is characterized in that it includes reaction record subsystem, agent delivery
The sequenator babinet of system and control system and the above-mentioned each system of carrying, wherein, reaction record subsystem and reagent
Supply system is separately positioned in left and right two parts section of sequenator babinet, and the agent delivery pipe by being arranged in babinet
Road connects；The rear side of reaction record subsystem of the set-up of control system on the left of the babinet in section, control reaction record
Subsystem and agent delivery system are acted according to scheduled sequential；
The sequenator babinet includes body supports component, the interior lining panel component being arranged on the inside of body supports component, left side
Door assembly component, offside door assembly component, buffer solution box installed unload platform assembly, left side cover plate assembly, rear side cover plate assembly, on
Cover board；
Babinet is divided into left and right two parts, reaction record subsystem is provided in the babinet of left side, including reaction warehouse component and CCD phases
Machine；Agent delivery system is provided in right case, including pump valve pipe integrated unit, liquid case hoistable platform and display,
Control assembly is provided on rear side of babinet, is adjusted to the temperature for detecting reaction tank and according to the threshold value of setting；
Reagent delivery to reacting in record subsystem, is reacted record subsystem by the control system control agent delivery system
Reaction warehouse component is inside provided with, reagent flows through the reaction chip being arranged in reaction warehouse component and reacts, and reaction record point is
CCD cameras in system acquire the photo in reaction process and obtain surveyed DNA sequences after analysis；
The interior lining panel component includes left side storehouse interior finishing panel assembly, right side storehouse left side interior finishing panel assembly and right side storehouse right side trim panel
Component；
Left side storehouse interior finishing panel assembly includes being arranged on the first intermediate trim panel and is separately positioned on the first trim panel two
Side and the second trim panel connected to it and third trim panel are also associated with the first interior trim on the outside of the second trim panel and mend slab
Slab is mended with the second interior trim.
2. the DNA sequencer with sequencing babinet according to claim 1, which is characterized in that the body supports group
Bottom, side, rear portion and the cutting part for controlling two housing areas of part supporting box；
Body supports component is made of steel plate splicing, is fixed by way of splicing or screw fastening；It further includes pedestal group
Part, top plate member connect and compose the support frame assembly of babinet side frame, buffer system mounting base with base assembly；
Wherein, the support frame assembly includes left side vertical plate component, the intermediate riser component for separating left and right two tanks, right side
Riser component and be arranged on right side riser forward portion right side mend slab, rear side riser component, be arranged in top plate member
The first cable fixed seat and the second cable fixed seat.
3. the DNA sequencer with sequencing babinet according to claim 2, which is characterized in that the reaction record point
System includes reaction warehouse component, motion platform component, motion control unit, CCD camera and platform support component, wherein, it is described
Reaction warehouse component, reaction chip component is arranged inside, under the action of control system, reaction solution, which enters in reaction chip, to carry out
Chemical reaction generates visible ray；
CCD camera, is fed into appropriate picture-taking position by the motion platform component, so that CCD camera is to reaction chip
The visible optical information of middle generation carries out acquisition of taking pictures；The platform component, the support reaction warehouse component, movement
Platform assembly；The motion control unit controls the operation of CCD camera and reaction chip in real time, and drive motion is put down
Platform component movement detects and controls the temperature of reaction warehouse to the picture-taking position of CCD cameras.
4. the DNA sequencer with sequencing babinet according to claim 2, which is characterized in that the left side vertical plate group
Part, intermediate riser component surround the reaction warehouse accommodating cavity for accommodating reaction warehouse component；The first cable is set to consolidate in top plate member
Left side babinet is divided into the reaction warehouse accommodating cavity of front side and the circuit board accommodating cavity of rear side by reservation, the steel plate；Intermediate riser group
Part and right side riser component surround reagent chamber.
5. the DNA sequenators with sequencing babinet according to claim 4, which is characterized in that the top plate group
Part including front side board, right plate, back side panel, left plate, is sequentially connected with, and each plate is successively between above-mentioned each plate
Bending plate, including vertical plate and level board, vertical plate is arranged on the outside of level board, and the front and rear connection of level board forms support portion
Point；
Intermediate plate is connected between front side board and back side panel, isolation board is provided between intermediate plate and left plate；In centre
Fluid path accommodating cavity is formed between plate and right plate, reaction warehouse accommodating cavity is formed between front side board and isolation board.
6. the DNA sequencer with sequencing babinet according to claim 4, which is characterized in that the reaction warehouse component
Including the vertical door frame being arranged on the platform component, the top in the outside of door frame is provided with turnable reaction
Storehouse component is connected with turnable basement in the lower end that reaction warehouse component may be reversed, and turnable basement is fixed on the middle part of door frame,
Reaction warehouse component, which may be reversed, to be rotated around the turnable basement, to take out or be put into reaction chip；
The turnable reaction warehouse component includes warehouse may be reversed, and reaction chip group is provided on the inside of warehouse may be reversed
Part, is equipped with reaction chip in reaction chip component, and reagent solution is chemically reacted by reaction chip.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510731379.8A CN105420071B (en) | 2015-11-02 | 2015-11-02 | A kind of DNA sequencer with sequencing babinet |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510731379.8A CN105420071B (en) | 2015-11-02 | 2015-11-02 | A kind of DNA sequencer with sequencing babinet |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105420071A CN105420071A (en) | 2016-03-23 |
| CN105420071B true CN105420071B (en) | 2018-06-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510731379.8A Expired - Fee Related CN105420071B (en) | 2015-11-02 | 2015-11-02 | A kind of DNA sequencer with sequencing babinet |
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| CN (1) | CN105420071B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105916347B (en) * | 2016-05-14 | 2018-09-28 | 新昌县羽林街道正名轴承厂 | A kind of electrical use device for carrying out releasing function automatically |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703311B (en) * | 2012-05-24 | 2013-08-21 | 中国科学院北京基因组研究所 | Reaction bin for adaptive regulation of DNA (Deoxyribose Nucleic Acid) sequencer |
| CN102703302B (en) * | 2012-05-24 | 2013-11-27 | 中国科学院北京基因组研究所 | Installing seat for automatically clamping sequencing chip |
| CN102766574B (en) * | 2012-05-24 | 2013-12-25 | 中国科学院北京基因组研究所 | Reaction chamber for DNA sequenator |
| CN102703312B (en) * | 2012-05-24 | 2014-03-19 | 中国科学院北京基因组研究所 | DNA (Deoxyribose Nucleic Acid) sequencer |
| CN105316228B (en) * | 2015-10-30 | 2016-11-16 | 北京中科紫鑫科技有限责任公司 | A kind of box DNA sequencer with straight-line feed CCD camera |
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2015
- 2015-11-02 CN CN201510731379.8A patent/CN105420071B/en not_active Expired - Fee Related
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Granted publication date: 20180612 Termination date: 20191102 |