CN105106725A - Swordtail bacillary enteritis preventing feed additive and preparation method thereof - Google Patents

Abstract

The invention relates to a swordtail bacillary enteritis preventing feed additive and a preparation method thereof. The swordtail bacillary enteritis preventing feed additive comprises, by mass, 28-35 parts of water extract of root of single roxburgh rose , 17-22 parts of juice of lawn pennywort herb, 15-21 parts of saxifraga, 15-20 parts of rhizome of featherleaf , 14-20 parts of root of nakedstamen monkshood, 13-20 parts of Chinese pulsatilla root, 13-20 parts of creeping woodsorrel herb, 9-17 parts of herb or fruit of little groundcherry, 9-16 parts of shrubalthea bark, 7-13 parts of fish oil, 13-19 parts of dry powder of aquatic earthworm, 13-19 parts of dry powder of clam worm, 5-10 parts of honey and the like. The Chinese medicinal herb feed additive is capable of effectively reducing incidence of swordtail bacillary enteritis and improving immunity and survival rate of swordtails.

Description

Prevent feed additive of red swordfish bacterial enteritis and preparation method thereof

Technical field

The invention belongs to technical field of aquaculture, particularly relate to a kind of feed additive preventing red swordfish bacterial enteritis; The invention still further relates to the preparation method of the feed additive of the red swordfish bacterial enteritis of a kind of above-mentioned prevention.

Background technology

Along with the growth in the living standard of people, material life is enriched greatly, and the requirement that people live to amusement and recreation is more and more obvious, and staying at home or supporting pet fish beautiful in colour a bit in garden is best selection, takes up room hardly because raise pet fish; Economic cost can be determined according to people; Condition is relatively simple, has water.Cultivation of ornamental fish not only can cultivate the body and the mind, and cultivate one's taste, the anion discharged in water and steam also can filter and wet air, build good living environment to people.

Red swordfish body subfusiform.The original species body colour of occurring in nature is pale bluish green.Milter tail fin lower edge extends a needle-like fin ray, is commonly called as sword tail, and raun is without sword tail, and Xiphophorus helleri is indispensable kind in aquarium.By biological engineering, its body colour is abundanter, has red, white, blue etc.It represents fish red sword, blue or green sword, Bai Jian, the red sword of black fin, the white sword of black fin, Aicis galericulata sword etc.Its body is strong, easily raises, and no matter is that slightly acidic water or week-base water can adapt to.Omnivorousness, Lumbricus, blood worm, man-made feeds all can be fed.Cold hardiness is stronger.Well-grown in the water temperature of 20 degree, is suitable for raising water temperature 24 ~ 26 degree, and its frightened rear happiness is jumped, and spring is very strong, for safety, preferably adds a cover plate on aquarium top.

Red swordfish bacterial enteritis that the cause of disease has mainly eaten uncleanly food or satiety of ingesting, intestinal is swollen is full, excretion is obstructed, finally cause enteritis by bacteriological infection intestinal, its pathogen is point-like aerogenesis pseudomonas bacillus.Its cardinal symptom is: sick fish usually abdominal part is expanded, and presents erythema, and anus is given prominence to, and fish body is dull, is parked in aquarium corner motionless, and body flesh does the tic of short time, does not take food, feces white; Cut fish belly open, visible hydroabdomen, the congested inflammation of intestinal wall, the lighter only partial enteral occurs red, and time serious, full intestinal is aubergine, and enteral, without food, is filled with flaxen mucus and blood pus.There is no the medicine of the red swordfish bacterial enteritis of special for treating at present, treat the common method for the treatment of of red swordfish bacterial enteritis for dipping bath of splashing with conventional sterilization antibiotic medicine such as nitrofural, gentamycin or oxytetracyclines, its shortcoming is that therapeutic effect is not good, toxicity is larger, while sterilization is prevented or cure a disease, larger damage be also result in red swordfish, and environment is easily polluted.Therefore, develop a kind of environmental protection, the feedstuff that effectively can prevent red swordfish bacterial enteritis or feed additive, there is important economic implications and market prospect.

Summary of the invention

An object of the present invention is to provide a kind of feed additive preventing red swordfish bacterial enteritis, its raw material components is green pure natural material, good bacteriostasis and sterilization effect is had to the pathogen of red swordfish bacterial enteritis, effectively can reduce the sickness rate of red swordfish bacterial enteritis, improve immunity and the survival rate of red swordfish.

In order to solve the problems of the technologies described above, the present invention by the following technical solutions, a kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 28-35 part, Herba Hydrocotyles juice 17-22 part, Herba Saxifragae 15-21 part, Serpentis pimple 15-20 part, Herba Cirsii juice 15-20 part, Semen Myristicae 16-21 part, Radix aconiti gymnandri 14-20 part, Radix Pulsatillae 13-20 part, Herba Oxalidis Corniculatae 13-20 part, Fructus Gardeniae 13-19 part, Radix Gentianae 13-18 part, Fructus Forsythiae 13-18 part, Herba Wahlenbergiae 12-20 part, Cortex Mori 11-19 part, Fructus Cnidii 12-19 part, Rhizoma Osmundae 11-18 part, Galla Chinensis 13-17 part, Spica Prunellae 12-19 part, Bulbus Allii 11-17 part, Fructus Sophorae 11-18 part, Fructus Gleditsia 9-18 part, Fructus Crataegi 8-18 part, Fructus Seu Herba Pubescentis 9-17 part, Cortex Hibisci 9-16 part, Mazus japonicus 8-14 part, bamboo salt 8-13 part, white vinegar 6-11 part, fish oil 7-13 part, dry red line worm powder 13-19 part, dry clam worm powder 13-19 part, Mel 5-10 part.

Preferably, the feed additive of the red swordfish bacterial enteritis of described prevention, be made up of each component of following masses number: Radix Rosae Normalis infusion 31-33 part, Herba Hydrocotyles juice 18-20 part, Herba Saxifragae 17-18 part, Serpentis pimple 16-18 part, Herba Cirsii juice 17-18 part, Semen Myristicae 18-20 part, Radix aconiti gymnandri 16-17 part, Radix Pulsatillae 15-17 part, Herba Oxalidis Corniculatae 15-17 part, Fructus Gardeniae 15-17 part, Radix Gentianae 15-16 part, Fructus Forsythiae 15-16 part, Herba Wahlenbergiae 14-16 part, Cortex Mori 14-15 part, Fructus Cnidii 14-15 part, Rhizoma Osmundae 13-15 part, Galla Chinensis 14-16 part, Spica Prunellae 14-15 part, Bulbus Allii 13-15 part, Fructus Sophorae 13-15 part, Fructus Gleditsia 13-15 part, Fructus Crataegi 12-14 part, Fructus Seu Herba Pubescentis 12-14 part, Cortex Hibisci 11-13 part, Mazus japonicus 10-12 part, bamboo salt 10-11 part, white vinegar 8-9 part, fish oil 9-11 part, dry red line worm powder 15-17 part, dry clam worm powder 15-17 part, Mel 6-8 part.

Preferably, the feed additive of the red swordfish bacterial enteritis of described prevention, be made up of each component of following masses number: Radix Rosae Normalis infusion 32 parts, Herba Hydrocotyles juice 19 parts, Herba Saxifragae 18 parts, Serpentis pimple 17 parts, Herba Cirsii juice 17 parts, Semen Myristicae 19 parts, Radix aconiti gymnandri 16 parts, the Radix Pulsatillae 16 parts, Herba Oxalidis Corniculatae 16 parts, Fructus Gardeniae 16 parts, Radix Gentianae 15 parts, Fructus Forsythiae 15 parts, Herba Wahlenbergiae 15 parts, Cortex Mori 15 parts, Fructus Cnidii 14 parts, Rhizoma Osmundae 14 parts, Galla Chinensis 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, the Fructus Sophorae 14 parts, 14 parts, Fructus Gleditsia, Fructus Crataegi 13 parts, Fructus Seu Herba Pubescentis 13 parts, Cortex Hibisci 12 parts, Mazus japonicus 11 parts, bamboo salt 11 parts, white vinegar 9 parts, 10 parts, fish oil, 16 parts dry, red line worm powder, dry clam worm powder 16 parts, Mel 7 parts.

Another object of the present invention is to provide the preparation method of the feed additive of the red swordfish bacterial enteritis of a kind of above-mentioned prevention, and the preparation method of the feed additive of the red swordfish bacterial enteritis of this prevention comprises the following steps:

(1) take Radix Rosae Normalis by proportioning, add the purified water that parts by weight are 3 times of Radix Sanguisorbae parts by weight, at 70-80 DEG C, 12-24h is soaked in water-bath, filters to obtain Radix Rosae Normalis infusion;

(2) fresh Herba Hydrocotyles smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Hydrocotyles juice, takes Herba Hydrocotyles juice according to proportioning;

(3) Herba Cirsii smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;

(4) take Herba Saxifragae and Serpentis pimple by proportioning, the gross weight of Herba Saxifragae and Serpentis pimple is denoted as 1 part, takes the purified water of 6 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;

(5) boil 2h after the soak that step (4) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;

(6) Semen Myristicae, Radix aconiti gymnandri, the Radix Pulsatillae, Herba Oxalidis Corniculatae, Fructus Gardeniae, Radix Gentianae, Fructus Forsythiae, Herba Wahlenbergiae, Cortex Mori, Fructus Cnidii, Rhizoma Osmundae, Galla Chinensis, Spica Prunellae, Bulbus Allii, the Fructus Sophorae, Fructus Gleditsia, Fructus Crataegi, Fructus Seu Herba Pubescentis, Cortex Hibisci and Mazus japonicus is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;

(7) grounds travel that extracting solution step (5) obtained and step (6) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;

(8) bamboo salt, white vinegar, fish oil, dry red line worm powder, dry clam worm powder and Mel is taken according to proportioning, mix homogeneously with the Radix Rosae Normalis infusion of step (1) gained, the Herba Hydrocotyles juice of step (2) gained, the Herba Cirsii juice of step (3) gained, the extract of step (7) gained, obtain object.

The preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.The advantage of vacuum lyophilization is thoroughly dry, can not make the active substance inactivation in red line polypide in dry process.

The preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.The advantage of vacuum lyophilization is thoroughly dry, can not make the active substance inactivation in clam worm body in dry process.

The using method of the feed additive of prevention of the present invention red swordfish bacterial enteritis is: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the feed additive of red for prevention of the present invention swordfish bacterial enteritis, preferably, the feed additive of prevention of the present invention red swordfish bacterial enteritis and the weight ratio of commercially available normal feedstuff are 1:20.

Each component effect of the present invention is resolved as follows:

Radix Rosae Normalis is the root of Fructus Rosae Normalis plant, is the root of rosaceous plant Fructus Rosae Normalis, nontoxic, and energy stomach invigorating, helps digestion, antidiarrheal, and arresting seminal emission, cures mainly stomachache, has loose bowels, dysentery, seminal emission, metrorrhagia, leukorrhagia, chronic cough.Herba Hydrocotyles is a kind of Umbelliferae Herba Hydrocotyles subordinate, and belong to the wild Herba Coriandri of the green type of nuisanceless health care, all herbal medicine, heat clearing away, diuresis, detumescence, removing toxic substances, subcutaneous ulcer of harnessing the Yellow River, dysentery, corneal nebula, larynx are swollen, carbuncle furuncle, traumatic injury injury with blood-stasis; In vitro tests has comparatively high inhibition effect to staphylococcus aureus, and to Bacillus proteus, shigella flexneri, Bacillus typhi also have the inhibitory action of not degree; Plague model test result, it is 54% that Mus mould suppresses, and by row malaria the selection result during Herba Hydrocotyles herb 100g/kg, suppression ratio is 54%.Herba Saxifragae has another name called stone lotus, gold thread hangs Hibisci Mutabilis, tiger ear etc., can dispel the wind, heat clearing away, removing pathogenic heat from blood and toxic substance from the body, can be used for treating rubella; Eczema, otitis media, erysipelas, cough is spitted blood, lung abscess, metrorrhagia, haemorrhoids.Serpentis pimple energy anti-inflammtory anti-dysentery, menstruction regulating and pain relieving, hemostasis, can be used for enteritis, bacillary dysentery, menoxenia, dysmenorrhea, rheumatalgia, fracture, traumatic injury swelling and pain due to blood stasis.Little Artichoke leaf is sweet, cool in nature, energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, and herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Semen Myristicae is Nutmeg aiphyllium plant, dungarunga; Sprout is elongated, and this kind is the famous spice in the torrid zone and medicinal plants, and gather when winter, spring two season fruit maturation, its kernel is used as medicine, and has sterilization antibacterial action, can control diarrhea of deficiency type cold-dysentery, coldness and pain in the epigastrium, vomiting etc.; External can make parasite expellent, treatment rheumatalgia etc.Radix aconiti gymnandri is warm in nature, bitter in the mouth, pungent, has effect of promoting blood circulation to restore menstrual flow, inducing diuresis to remove edema, can treat menoxenia, amenorrhea, stimulate the menstrual flow, the disease such as edema, postnatal blood stasis stomachache, and herb can hyoscine, has stronger toxic action.The Radix Pulsatillae can treat the bleedings such as hemorrhoid, controls toxic-heat and blood stasis, pyrexial malaria cold and heat, epistaxis, internal hemorrhoid with bleeding, and the research such as Lan Jiyu finds that Radix Pulsatillae decoct is better to the effect killing Entamoeba histolytica; Cao Jinghua etc. record Radix Pulsatillae Aqueous extracts with K-B disk diffusion method and have obvious inhibitory action to staphylococcus aureus, bacillus pyocyaneus, anthrax bacillus, Bacillus typhi, alpha streptococcus, group B streptococcus.Herba Oxalidis Corniculatae all herbal medicine, energy bactericidal antiphlogistic, antipyretic diuresis, eliminating stagnated blood to disperse swelling, traumatic injury.Fructus Gardeniae can heat clearing away, pathogenic fire purging, and removing heat from blood, can be used for the treatment of calentura restlessness of asrhenia type and insomnia, jaundice, gonorrhea, quench one's thirst, conjunctival congestion, pharyngalgia, and spit blood, epistaxis, dysentery, hematuria, pathopyretic ulcer, sprains and swell and ache.Radix Gentianae is root and the stem of Gentianaceae herbaceos perennial Gentiana manshurica etc., main product the Northeast, and spring, Qiu Erji all can gather, and are good with the autumn person of adopting quality, and root and rhizome is used as medicine, bitter in the mouth, cold in nature, the special heat clearing and damp drying of merit, heat clearing and damp drying; Eliminating pathogen in the liver is calmed the frightened, and cures mainly jaundice due to damp-heat; Urine leaching pain; Swelling of the vulva pudendal pruritus; Damp-heat vaginal discharge; The feeling of fullness in the head headache of liver and gall excess-fire; Conjunctival congestion and swelling pain; Deaf ear swells; Hypochondriac pain bitter taste; Calentura convulsion with spasms.The heat clearing away of Fructus Forsythiae energy, removing toxic substances, eliminating stagnation, detumescence, can be used for treatment warm, erysipelas, macule, carbuncle and ulcer toxic swelling, scrofula, urine stranguria with urinary obstruction.Herba Wahlenbergiae tonifying the spleen and stomach, lung benefiting kidney, eliminates the phlegm, parasite killing, hemostasis, and heat clearing away of inducing sweat is controlled wind heat and breathed heavily and cough, hemoptysis, epistaxis, infantile convulsion, traumatic injury.The extract of the fat-soluble solvent such as petroleum ether, ether, ethyl acetate of Cortex Mori, to the quiet note of mice, can reduce the spontaneous activity of mice, and have anticonvulsant effect; The Cortex Mori severe edema due to hypofunction of the spleen or the above intraperitoneal injection of mice of n-butanol extract 50mg/kg, can make animal spontaneous activity reduce, and sense of touch and pain sensation reaction reduce, platycoria, and the generation that slightly can suppress mice galvanic shock; Cortex Mori water extract has inhibitory action to the swollen feet caused by Oral Administration in Rats or injection on Carrageenan and glucosan; Cortex Mori decoct has stronger inhibitory action to staphylococcus aureus; Also slight inhibitory action is had to Bacillus typhi and shigella flexneri; Cortex Mori furan A all has stronger bacteriostatic activity to staphylococcus aureus, mycobacterium; Cyclomorusin also has antifungic action.Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri, has resisting pathogenic microbes effect, and Fructus Cnidii all has inhibitory action to acrothesium floccosum, Gypsum Fibrosum sample microspore bacterium, ulotrichy microspore bacterium; Also there is antifungic action, Antifungal activities, insecticidal action.Rhizoma Osmundae is rhizome and the petiole residual base of Dryopteridaceae plant dryopteris crassirhizoma, can be used for treating anemopyretic cold; Warm skin eruption; Spit blood; Hemoptysis; Epistaxis; Have blood in stool; Metrorrhagia; Dysentery; The intestinal parasitic diseases such as leukorrhagia and hook, ascarid, cestode.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.The decoct of Spica Prunellae, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of Spica Prunellae, has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, and also have warming the spleen warming the stomach in addition, control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension.The Fructus Sophorae is dry flower and alabastrum, the mature fruit of leguminous plant Chinese scholartree, energy clearing away heat-fire, cooling blood for hemostasis, and can be used for intestinal heat and have blood in stool, hemorrhoid swell hemorrhage, and liver-heat is had a headache, dizzy conjunctival congestion etc.Fructus Gleditsia has the effect of parasite killing, removing toxic substances: saponin element contained in Fructus Gleditsia is triterpene class and oligosaccharide, has antiinflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as acquired immune deficiency syndrome (AIDS) immunity.Fructus Crataegi is the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, and the disease such as, hernia, blood stasis full to chest and diaphragm spleen, amenorrhea has good curative effect.Fructus Seu Herba Pubescentis all herbal medicine, controls the diseases such as dysfunctional uterine hemorrhage, cholecystitis, jaundice type hepatitis, headache due to common cold, stomachache, infantile malnutrition, acute catarrhal conjunctivitis, traumatic injury, treatment skin ulcer, sores ulceration of skin, Serpentis and lyssodexis, tinea pedis, the treatment of rotten head and hemorrhoid.Cortex Hibisci energy clearing away heat-damp and promoting diuresis, killing parasites for relieving itching, can be used for treating dysentery, leucorrhea; Eczema scrotum tinea corporis is controlled in external, tinea pedis.The herb of Mazus japonicus can be used for pain relieving, stomach invigorating, and removing toxic substances and promoting subsidence of swelling can be used for migraine, dyspepsia; Furuncle is controlled in external, impetigo, scalds.Bamboo salt is evaporated brine day to load in triennial QINGZHU, and two ends are sealed with natural loess, take pinaster as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, antiinflammatory, toxopexic effect.White vinegar has the function of dissipating blood stasis, removing toxic substances, detumescence.Oleum menthae light weight a surname is loose, has dispelling wind and heat pathogens, declares the merit of malicious rash: can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Fish oil can provide energy for red sword, and has food calling effect.Red line worm can be used as the high-quality animal feed of aquatic animal, can reduce the production cost of feedstuff, improves culture benefit and forms quality.Clam worm delicious flavour, nutritious, the multiple nutritional components such as its rich in proteins and calcium, phosphorus, ferrum, iodine, and there is higher medicinal and food therapy value, property is salty-cold, has nourishing YIN to lower pathogenic fire, and the function of tonify deficiency lung heat clearing has blood pressure lowering, controls wet, protective effect on cancer risk.It is cold in nature, sweet in the mouth, salty, has effect of nourishing YIN to lower pathogenic fire, lung heat clearing tonify deficiency.Mel can loosening bowel to relieve constipation, strength can be made up rapidly, eliminate tired out, build up one's resistance to disease, can antibacterial sterilizing, and feedstuff mouthfeel can be improved.

In preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on red swordfish, promotes that red swordfish is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.

The feed additive of the red swordfish bacterial enteritis of prevention of the present invention, the mechanism of action according to bacterial enteritis, the suitable component selected, and organically combined, there is significant cooperative synergism effect, play the function of each component to greatest extent, and the component of the feed additive of the red swordfish bacterial enteritis of prevention of the present invention is environmental protection raw material.Because the fish oil contained in feed additive of the present invention has food calling effect, red line worm and clam worm delicious flavour, Mel can improve the overall mouthfeel of feedstuff, therefore the feed additive of the red swordfish bacterial enteritis of prevention of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of red swordfish, induce red swordfish to ingest and add the normal feedstuff of feed additive of the present invention, therefore red swordfish immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen point-like aerogenesis pseudomonas bacillus of bacterial enteritis to remarkable bactericidal and bacteriostatic effect can enter in red swordfish body, and play it and act on accordingly.

Compared with prior art, the invention has the beneficial effects as follows:

(1) Radix Rosae Normalis is the root of Fructus Rosae Normalis plant, is the root of rosaceous plant Fructus Rosae Normalis, nontoxic, and energy stomach invigorating, helps digestion, antidiarrheal, and arresting seminal emission, cures mainly stomachache, has loose bowels, dysentery, seminal emission, metrorrhagia, leukorrhagia, chronic cough.Herba Hydrocotyles is a kind of Umbelliferae Herba Hydrocotyles subordinate, and belong to the wild Herba Coriandri of the green type of nuisanceless health care, all herbal medicine, heat clearing away, diuresis, detumescence, removing toxic substances, subcutaneous ulcer of harnessing the Yellow River, dysentery, corneal nebula, larynx are swollen, carbuncle furuncle, traumatic injury injury with blood-stasis; In vitro tests has comparatively high inhibition effect to staphylococcus aureus, and to Bacillus proteus, shigella flexneri, Bacillus typhi also have the inhibitory action of not degree; Plague model test result, it is 54% that Mus mould suppresses, and by row malaria the selection result during Herba Hydrocotyles herb 100g/kg, suppression ratio is 54%.Herba Saxifragae has another name called stone lotus, gold thread hangs Hibisci Mutabilis, tiger ear etc., can dispel the wind, heat clearing away, removing pathogenic heat from blood and toxic substance from the body, can be used for treating rubella; Eczema, otitis media, erysipelas, cough is spitted blood, lung abscess, metrorrhagia, haemorrhoids.Serpentis pimple energy anti-inflammtory anti-dysentery, menstruction regulating and pain relieving, hemostasis, can be used for enteritis, bacillary dysentery, menoxenia, dysmenorrhea, rheumatalgia, fracture, traumatic injury swelling and pain due to blood stasis.Little Artichoke leaf is sweet, cool in nature, energy cooling blood for hemostasis, removing toxicity for eliminating carbuncles, clearing heat and relieving fidgetness, Herba Cirsii Herb is containing compositions such as choline, Catecholamine matter, Saponin, alkaloids, and herb, containing compositions such as volatile oil, inulin, alkaloid, flavonoid, coumarin derivatives, all has inhibitory action to Diplococcus pneumoniae, Hemolytic streptococcus, diphtheria corynebacterium, typhoid fever village bacterium, bacillus pyocyaneus, dysentery bacterium, staphylococcus aureus etc.Semen Myristicae is Nutmeg aiphyllium plant, dungarunga; Sprout is elongated, and this kind is the famous spice in the torrid zone and medicinal plants, and gather when winter, spring two season fruit maturation, its kernel is used as medicine, and has sterilization antibacterial action, can control diarrhea of deficiency type cold-dysentery, coldness and pain in the epigastrium, vomiting etc.; External can make parasite expellent, treatment rheumatalgia etc.Radix aconiti gymnandri is warm in nature, bitter in the mouth, pungent, has effect of promoting blood circulation to restore menstrual flow, inducing diuresis to remove edema, can treat menoxenia, amenorrhea, stimulate the menstrual flow, the disease such as edema, postnatal blood stasis stomachache, and herb can hyoscine, has stronger toxic action.The Radix Pulsatillae can treat the bleedings such as hemorrhoid, controls toxic-heat and blood stasis, pyrexial malaria cold and heat, epistaxis, internal hemorrhoid with bleeding, and the research such as Lan Jiyu finds that Radix Pulsatillae decoct is better to the effect killing Entamoeba histolytica; Cao Jinghua etc. record Radix Pulsatillae Aqueous extracts with K-B disk diffusion method and have obvious inhibitory action to staphylococcus aureus, bacillus pyocyaneus, anthrax bacillus, Bacillus typhi, alpha streptococcus, group B streptococcus.Herba Oxalidis Corniculatae all herbal medicine, energy bactericidal antiphlogistic, antipyretic diuresis, eliminating stagnated blood to disperse swelling, traumatic injury.Fructus Gardeniae can heat clearing away, pathogenic fire purging, and removing heat from blood, can be used for the treatment of calentura restlessness of asrhenia type and insomnia, jaundice, gonorrhea, quench one's thirst, conjunctival congestion, pharyngalgia, and spit blood, epistaxis, dysentery, hematuria, pathopyretic ulcer, sprains and swell and ache.Radix Gentianae is root and the stem of Gentianaceae herbaceos perennial Gentiana manshurica etc., main product the Northeast, and spring, Qiu Erji all can gather, and are good with the autumn person of adopting quality, and root and rhizome is used as medicine, bitter in the mouth, cold in nature, the special heat clearing and damp drying of merit, heat clearing and damp drying; Eliminating pathogen in the liver is calmed the frightened, and cures mainly jaundice due to damp-heat; Urine leaching pain; Swelling of the vulva pudendal pruritus; Damp-heat vaginal discharge; The feeling of fullness in the head headache of liver and gall excess-fire; Conjunctival congestion and swelling pain; Deaf ear swells; Hypochondriac pain bitter taste; Calentura convulsion with spasms.The heat clearing away of Fructus Forsythiae energy, removing toxic substances, eliminating stagnation, detumescence, can be used for treatment warm, erysipelas, macule, carbuncle and ulcer toxic swelling, scrofula, urine stranguria with urinary obstruction.Herba Wahlenbergiae tonifying the spleen and stomach, lung benefiting kidney, eliminates the phlegm, parasite killing, hemostasis, and heat clearing away of inducing sweat is controlled wind heat and breathed heavily and cough, hemoptysis, epistaxis, infantile convulsion, traumatic injury.The extract of the fat-soluble solvent such as petroleum ether, ether, ethyl acetate of Cortex Mori, to the quiet note of mice, can reduce the spontaneous activity of mice, and have anticonvulsant effect; The Cortex Mori severe edema due to hypofunction of the spleen or the above intraperitoneal injection of mice of n-butanol extract 50mg/kg, can make animal spontaneous activity reduce, and sense of touch and pain sensation reaction reduce, platycoria, and the generation that slightly can suppress mice galvanic shock; Cortex Mori water extract has inhibitory action to the swollen feet caused by Oral Administration in Rats or injection on Carrageenan and glucosan; Cortex Mori decoct has stronger inhibitory action to staphylococcus aureus; Also slight inhibitory action is had to Bacillus typhi and shigella flexneri; Cortex Mori furan A all has stronger bacteriostatic activity to staphylococcus aureus, mycobacterium; Cyclomorusin also has antifungic action.Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri, has resisting pathogenic microbes effect, and Fructus Cnidii all has inhibitory action to acrothesium floccosum, Gypsum Fibrosum sample microspore bacterium, ulotrichy microspore bacterium; Also there is antifungic action, Antifungal activities, insecticidal action.Rhizoma Osmundae is rhizome and the petiole residual base of Dryopteridaceae plant dryopteris crassirhizoma, can be used for treating anemopyretic cold; Warm skin eruption; Spit blood; Hemoptysis; Epistaxis; Have blood in stool; Metrorrhagia; Dysentery; The intestinal parasitic diseases such as leukorrhagia and hook, ascarid, cestode.Galla Chinensis is cold in nature, sour in the mouth, puckery, has the effects such as pathogenic fire reducing of astringing the lung, relieving diarrhea with astringents, arresting sweating hemostasis, removing dampness sore, cures mainly the diseases such as chronic cough of deficiency lung, chronic diarrhea chronic dysentery, hemorrhoidal bleeding of having blood in stool, carbuncle sore tumefacting virus, Skin wet and rotten.The decoct of Spica Prunellae, water-leach liquor, alcohol-water leachate and alcohol leaching liquid all can obviously reduce laboratory animal blood pressure, and stem, leaf, fringe and herb all have hypotensive effect, but the effect of fringe is more obvious; The water decoction-alcohol sedimentation liquid mouse peritoneal injection of Spica Prunellae, has obvious antiinflammatory action; The decoct of Spica Prunellae all has certain inhibitory action to dysentery bacterium, Bacillus typhi, vibrio cholera, escherichia coli, Bacillus proteus, staphylococcus and Bacillus tuberculosis in vitro.Bulbus Allii removing toxic substances parasite killing, reducing swelling and alleviating pain, antidiarrheal dysentery relieving, controls lung, anthelmintic, and also have warming the spleen warming the stomach in addition, control swollen ulcer drug, white bald tinea skin ulcer, dysentery is had loose bowels, pulmonary tuberculosis pertussis, ascarid pinworm, eating accumulation, coldness and pain in the epigastrium, edema distension.The Fructus Sophorae is dry flower and alabastrum, the mature fruit of leguminous plant Chinese scholartree, energy clearing away heat-fire, cooling blood for hemostasis, and can be used for intestinal heat and have blood in stool, hemorrhoid swell hemorrhage, and liver-heat is had a headache, dizzy conjunctival congestion etc.Fructus Gleditsia has the effect of parasite killing, removing toxic substances: saponin element contained in Fructus Gleditsia is triterpene class and oligosaccharide, has antiinflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as acquired immune deficiency syndrome (AIDS) immunity.Fructus Crataegi is the good medicine of spleen benefiting and stimulating the appetite, relieving dyspepsia, phlegm reduction of blood circulation promoting, and the disease such as, hernia, blood stasis full to chest and diaphragm spleen, amenorrhea has good curative effect.Fructus Seu Herba Pubescentis all herbal medicine, controls the diseases such as dysfunctional uterine hemorrhage, cholecystitis, jaundice type hepatitis, headache due to common cold, stomachache, infantile malnutrition, acute catarrhal conjunctivitis, traumatic injury, treatment skin ulcer, sores ulceration of skin, Serpentis and lyssodexis, tinea pedis, the treatment of rotten head and hemorrhoid.Cortex Hibisci energy clearing away heat-damp and promoting diuresis, killing parasites for relieving itching, can be used for treating dysentery, leucorrhea; Eczema scrotum tinea corporis is controlled in external, tinea pedis.The herb of Mazus japonicus can be used for pain relieving, stomach invigorating, and removing toxic substances and promoting subsidence of swelling can be used for migraine, dyspepsia; Furuncle is controlled in external, impetigo, scalds.Bamboo salt is evaporated brine day to load in triennial QINGZHU, and two ends are sealed with natural loess, take pinaster as fuel, the material extract after 1000 DEG C ~ 1500 DEG C high-temperature calcinations, has antibacterial, antiinflammatory, toxopexic effect.White vinegar has the function of dissipating blood stasis, removing toxic substances, detumescence.Oleum menthae light weight a surname is loose, has dispelling wind and heat pathogens, declares the merit of malicious rash: can be used for controlling wind heat bundle table, measles without adequate eruption; Also can treat rubella pruritus, get the effect that its rash of dispeling the wind is antipruritic.Fish oil can provide energy for red sword, and has food calling effect.Red line worm can be used as the high-quality animal feed of aquatic animal, can reduce the production cost of feedstuff, improves culture benefit and forms quality.Clam worm delicious flavour, nutritious, the multiple nutritional components such as its rich in proteins and calcium, phosphorus, ferrum, iodine, and there is higher medicinal and food therapy value, property is salty-cold, has nourishing YIN to lower pathogenic fire, and the function of tonify deficiency lung heat clearing has blood pressure lowering, controls wet, protective effect on cancer risk.It is cold in nature, sweet in the mouth, salty, has effect of nourishing YIN to lower pathogenic fire, lung heat clearing tonify deficiency.Mel can loosening bowel to relieve constipation, strength can be made up rapidly, eliminate tired out, build up one's resistance to disease, can antibacterial sterilizing, and feedstuff mouthfeel can be improved.

(2) component of the feed additive of the red swordfish bacterial enteritis of prevention of the present invention is environmental protection raw material, environmental sound, uses safer.

(3) in preparation method, crude drug purified water soaking a period of time, medical material deliquescing, cell expansion, effective ingredient is easily fried, and medical material contains starch, protein, after soaking, prevent when decocting, the starch gelatinization on medical material surface, protein coagulating, the capillary channel on blocking medical material surface, make the moisture when decocting enter not go, effective ingredient is not easily fried, affects curative effect of medication; Decoction can change the irritation effect of medicine, relatively mildly acts on red swordfish, promotes that red swordfish is to the absorption of medicine; Grinding makes the starch in medical material, protein is separated out more fully at subsequent step; Supercritical extraction, immersion, concentration step retain the effective ingredient in Chinese medicine of the present invention to greatest extent.

(4) fish oil owing to containing in feed additive of the present invention has food calling effect, red line worm and clam worm delicious flavour, Mel can improve the overall mouthfeel of feedstuff, therefore the feed additive of the red swordfish bacterial enteritis of prevention of the present invention can improve the mouthfeel of normal feedstuff, stimulate the taste bud of red swordfish, the red swordfish of food calling ingests and adds the normal feedstuff of feed additive of the present invention, therefore red swordfish immunity and resistance can be strengthened in feed additive of the present invention, the composition pathogen point-like aerogenesis pseudomonas bacillus of bacterial enteritis to remarkable bactericidal and bacteriostatic effect can enter in red swordfish body, and play it and act on accordingly.

Detailed description of the invention

Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.

Embodiment 1

In the present invention's first detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 32 parts, Herba Hydrocotyles juice 19 parts, Herba Saxifragae 18 parts, Serpentis pimple 17 parts, Herba Cirsii juice 17 parts, Semen Myristicae 19 parts, Radix aconiti gymnandri 16 parts, the Radix Pulsatillae 16 parts, Herba Oxalidis Corniculatae 16 parts, Fructus Gardeniae 16 parts, Radix Gentianae 15 parts, Fructus Forsythiae 15 parts, Herba Wahlenbergiae 15 parts, Cortex Mori 15 parts, Fructus Cnidii 14 parts, Rhizoma Osmundae 14 parts, Galla Chinensis 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, the Fructus Sophorae 14 parts, 14 parts, Fructus Gleditsia, Fructus Crataegi 13 parts, Fructus Seu Herba Pubescentis 13 parts, Cortex Hibisci 12 parts, Mazus japonicus 11 parts, bamboo salt 11 parts, white vinegar 9 parts, 10 parts, fish oil, 16 parts dry, red line worm powder, dry clam worm powder 16 parts, Mel 7 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of above-mentioned prevention comprises the following steps:

(1) take Radix Rosae Normalis by proportioning, add the purified water that parts by weight are 3 times of Radix Sanguisorbae parts by weight, at 70-80 DEG C, 12-24h is soaked in water-bath, filters to obtain Radix Rosae Normalis infusion;

(2) fresh Herba Hydrocotyles smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Hydrocotyles juice, takes Herba Hydrocotyles juice according to proportioning;

(3) Herba Cirsii smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;

(4) take Herba Saxifragae and Serpentis pimple by proportioning, the gross weight of Herba Saxifragae and Serpentis pimple is denoted as 1 part, takes the purified water of 6 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;

(5) boil 2h after the soak that step (4) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;

(6) Semen Myristicae, Radix aconiti gymnandri, the Radix Pulsatillae, Herba Oxalidis Corniculatae, Fructus Gardeniae, Radix Gentianae, Fructus Forsythiae, Herba Wahlenbergiae, Cortex Mori, Fructus Cnidii, Rhizoma Osmundae, Galla Chinensis, Spica Prunellae, Bulbus Allii, the Fructus Sophorae, Fructus Gleditsia, Fructus Crataegi, Fructus Seu Herba Pubescentis, Cortex Hibisci and Mazus japonicus is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;

(7) grounds travel that extracting solution step (5) obtained and step (6) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;

(8) bamboo salt, white vinegar, fish oil, dry red line worm powder, dry clam worm powder and Mel is taken according to proportioning, mix homogeneously with the Radix Rosae Normalis infusion of step (1) gained, the Herba Hydrocotyles juice of step (2) gained, the Herba Cirsii juice of step (3) gained, the extract of step (7) gained, obtain object.

The preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.

The preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, then uses distilled water flushing three times, grinds to form powdery after vacuum lyophilization to finish-drying, crosses 80 mesh sieves.

Embodiment 2

In the present invention's second detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 35 parts, Herba Hydrocotyles juice 22 parts, Herba Saxifragae 21 parts, Serpentis pimple 20 parts, Herba Cirsii juice 20 parts, Semen Myristicae 20 parts, Radix aconiti gymnandri 20 parts, the Radix Pulsatillae 19 parts, Herba Oxalidis Corniculatae 19 parts, Fructus Gardeniae 19 parts, Radix Gentianae 18 parts, Fructus Forsythiae 18 parts, Herba Wahlenbergiae 18 parts, Cortex Mori 19 parts, Fructus Cnidii 19 parts, Rhizoma Osmundae 18 parts, Galla Chinensis 17 parts, Spica Prunellae 19 parts, 17 parts, Bulbus Allii, the Fructus Sophorae 18 parts, 18 parts, Fructus Gleditsia, Fructus Crataegi 16 parts, Fructus Seu Herba Pubescentis 17 parts, Cortex Hibisci 16 parts, Mazus japonicus 14 parts, bamboo salt 12 parts, white vinegar 10 parts, 13 parts, fish oil, 19 parts dry, red line worm powder, dry clam worm powder 19 parts, Mel 8 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of prevention of the present embodiment is with embodiment 1.

Embodiment 3

In the present invention's the 3rd detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 33 parts, Herba Hydrocotyles juice 20 parts, Herba Saxifragae 20 parts, Serpentis pimple 20 parts, Herba Cirsii juice 18 parts, Semen Myristicae 21 parts, Radix aconiti gymnandri 20 parts, the Radix Pulsatillae 20 parts, Herba Oxalidis Corniculatae 20 parts, Fructus Gardeniae 17 parts, Radix Gentianae 16 parts, Fructus Forsythiae 16 parts, Herba Wahlenbergiae 20 parts, Cortex Mori 15 parts, Fructus Cnidii 15 parts, Rhizoma Osmundae 15 parts, Galla Chinensis 16 parts, Spica Prunellae 15 parts, 15 parts, Bulbus Allii, the Fructus Sophorae 15 parts, 15 parts, Fructus Gleditsia, Fructus Crataegi 18 parts, Fructus Seu Herba Pubescentis 14 parts, Cortex Hibisci 13 parts, Mazus japonicus 12 parts, bamboo salt 13 parts, white vinegar 11 parts, 11 parts, fish oil, 18 parts dry, red line worm powder, dry clam worm powder 17 parts, Mel 10 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of prevention of the present embodiment is with embodiment 1.

Embodiment 4

In the present invention's the 4th detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 28 parts, Herba Hydrocotyles juice 18 parts, Herba Saxifragae 17 parts, Serpentis pimple 15 parts, Herba Cirsii juice 15 parts, Semen Myristicae 16 parts, Radix aconiti gymnandri 14 parts, the Radix Pulsatillae 13 parts, Herba Oxalidis Corniculatae 13 parts, Fructus Gardeniae 13 parts, Radix Gentianae 13 parts, Fructus Forsythiae 13 parts, Herba Wahlenbergiae 12 parts, Cortex Mori 11 parts, Fructus Cnidii 12 parts, Rhizoma Osmundae 11 parts, Galla Chinensis 13 parts, Spica Prunellae 12 parts, 11 parts, Bulbus Allii, the Fructus Sophorae 11 parts, 9 parts, Fructus Gleditsia, Fructus Crataegi 8 parts, Fructus Seu Herba Pubescentis 9 parts, Cortex Hibisci 9 parts, Mazus japonicus 8 parts, bamboo salt 8 parts, white vinegar 8 parts, 9 parts, fish oil, 15 parts dry, red line worm powder, dry clam worm powder 13 parts, Mel 5 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of prevention of the present embodiment is with embodiment 1.

Embodiment 5

In the present invention's the 5th detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 31 parts, Herba Hydrocotyles juice 17 parts, Herba Saxifragae 18 parts, Serpentis pimple 18 parts, Herba Cirsii juice 17 parts, Semen Myristicae 18 parts, Radix aconiti gymnandri 17 parts, the Radix Pulsatillae 17 parts, Herba Oxalidis Corniculatae 17 parts, Fructus Gardeniae 15 parts, Radix Gentianae 14 parts, Fructus Forsythiae 15 parts, Herba Wahlenbergiae 16 parts, Cortex Mori 14 parts, Fructus Cnidii 14 parts, Rhizoma Osmundae 13 parts, Galla Chinensis 14 parts, Spica Prunellae 14 parts, 13 parts, Bulbus Allii, the Fructus Sophorae 13 parts, 13 parts, Fructus Gleditsia, Fructus Crataegi 14 parts, Fructus Seu Herba Pubescentis 12 parts, Cortex Hibisci 11 parts, Mazus japonicus 10 parts, bamboo salt 11 parts, white vinegar 6 parts, 7 parts, fish oil, 17 parts dry, red line worm powder, dry clam worm powder 15 parts, Mel 6 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of prevention of the present embodiment is with embodiment 1.

Embodiment 6

In the present invention's the 6th detailed description of the invention, feed ingredient and preparation method thereof is as follows:

A kind of feed additive preventing red swordfish bacterial enteritis, be made up of each component of following masses number: Radix Rosae Normalis infusion 30 parts, Herba Hydrocotyles juice 22 parts, Herba Saxifragae 15 parts, Serpentis pimple 16 parts, Herba Cirsii juice 20 parts, Semen Myristicae 18 parts, Radix aconiti gymnandri 16 parts, the Radix Pulsatillae 15 parts, Herba Oxalidis Corniculatae 15 parts, Fructus Gardeniae 16 parts, Radix Gentianae 15 parts, Fructus Forsythiae 16 parts, Herba Wahlenbergiae 14 parts, Cortex Mori 16 parts, Fructus Cnidii 16 parts, Rhizoma Osmundae 16 parts, Galla Chinensis 15 parts, Spica Prunellae 14 parts, 17 parts, Bulbus Allii, the Fructus Sophorae 15 parts, 12 parts, Fructus Gleditsia, Fructus Crataegi 12 parts, Fructus Seu Herba Pubescentis 14 parts, Cortex Hibisci 13 parts, Mazus japonicus 10 parts, bamboo salt 10 parts, white vinegar 9 parts, 10 parts, fish oil, 13 parts dry, red line worm powder, dry clam worm powder 15 parts, Mel 8 parts.

The preparation method of the feed additive of the red swordfish bacterial enteritis of prevention of the present embodiment is with embodiment 1.

Experimental result

Culture experiment

With the group of normally feeding of normal feedstuff of throwing something and feeding for blank group, to add the feedstuff group of the embodiment of the present invention 1 in normal feedstuff for experimental group, often group establish 3 parallel, in experimental group, the feed additive of embodiment 1 and the weight ratio of normal feedstuff are 1:20.Choose 6 aquariums, healthy red swordfish 60, red for health sword is all assigned to 6 aquariums at random, each aquarium 10 red swordfishes, in each aquarium, add the red swordfish that 1 is suffered from bacterial enteritis, observe the growth of red swordfish and dead, infection conditions in feeding process, observe 45 days.The results are shown in Table 1:

The health status of the red swordfish of table 1 and the red swordfish of matched group

From table 1, the feed additive of prevention of the present invention red swordfish bacterial enteritis effectively can reduce the incidence rate of red swordfish bacterial enteritis.

Bacteriostatic test checks

Adopt nitrofural to do matched group, the feed additive of the prevention red swordfish bacterial enteritis of the embodiment of the present invention 1 is that experimental group carries out antibacterial inspection.

The preparation of culture medium: take in the water-soluble 800mL of beef extract 5g, adds peptone 10g and dissolves, and adds casein 34g and dissolves, then adds NaCl5g dissolving, is adjusted to pH7.6 ~ 7.8, is diluted to 1000mL.Add agar powder 18g, autoclaving 15min, when being cooled to 40 DEG C ~ 50 DEG C, a kind of rhyme scheme in Chinese operas serving as the prelude to a complete score for voices, solidifies, and the 4 DEG C of refrigerators placing no bacteria pollution are for subsequent use.

Strain is: point-like aerogenesis pseudomonas bacillus.

Matched group antibacterial liquid is prepared: be dissolved in respectively in 10ml sterile deionized water by 1 part of nitrofural, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.

Experimental group antibacterial liquid is prepared: be dissolved in 10ml sterile deionized water by the feed additive of red for the prevention of the embodiment of the present invention 1 swordfish bacterial enteritis, diluted respectively by liquor strength, with stock solution than being 1:2,1:4,1:8,1:16,1:32.To the fungistatic effect of myxobacter in table 2:

The bactericidal and bacteriostatic effect of the feed additive of the red swordfish bacterial enteritis of table 2 prevention of the present invention

From table 2, the pathogen point-like aerogenesis pseudomonas bacillus of feed additive to bacterial enteritis of the prevention red swordfish bacterial enteritis of embodiments of the invention 1 has good bactericidal and bacteriostatic effect.

The above is only the preferred embodiment of the present invention, is noted that for those skilled in the art, and under the premise without departing from the principles of the invention, can also make some improvement, these improvement should be considered as protection scope of the present invention.

Claims (8)

1. the feed additive of the red swordfish bacterial enteritis of prevention, it is characterized in that: be made up of each component of following masses number: Radix Rosae Normalis infusion 28-35 part, Herba Hydrocotyles juice 17-22 part, Herba Saxifragae 15-21 part, Serpentis pimple 15-20 part, Herba Cirsii juice 15-20 part, Semen Myristicae 16-21 part, Radix aconiti gymnandri 14-20 part, Radix Pulsatillae 13-20 part, Herba Oxalidis Corniculatae 13-20 part, Fructus Gardeniae 13-19 part, Radix Gentianae 13-18 part, Fructus Forsythiae 13-18 part, Herba Wahlenbergiae 12-20 part, Cortex Mori 11-19 part, Fructus Cnidii 12-19 part, Rhizoma Osmundae 11-18 part, Galla Chinensis 13-17 part, Spica Prunellae 12-19 part, Bulbus Allii 11-17 part, Fructus Sophorae 11-18 part, Fructus Gleditsia 9-18 part, Fructus Crataegi 8-18 part, Fructus Seu Herba Pubescentis 9-17 part, Cortex Hibisci 9-16 part, Mazus japonicus 8-14 part, bamboo salt 8-13 part, white vinegar 6-11 part, fish oil 7-13 part, dry red line worm powder 13-19 part, dry clam worm powder 13-19 part, Mel 5-10 part.

2. the feed additive of the red swordfish bacterial enteritis of prevention according to claim 1, it is characterized in that: the feed additive of the red swordfish bacterial enteritis of described prevention, be made up of each component of following masses number: Radix Rosae Normalis infusion 31-33 part, Herba Hydrocotyles juice 18-20 part, Herba Saxifragae 17-18 part, Serpentis pimple 16-18 part, Herba Cirsii juice 17-18 part, Semen Myristicae 18-20 part, Radix aconiti gymnandri 16-17 part, Radix Pulsatillae 15-17 part, Herba Oxalidis Corniculatae 15-17 part, Fructus Gardeniae 15-17 part, Radix Gentianae 15-16 part, Fructus Forsythiae 15-16 part, Herba Wahlenbergiae 14-16 part, Cortex Mori 14-15 part, Fructus Cnidii 14-15 part, Rhizoma Osmundae 13-15 part, Galla Chinensis 14-16 part, Spica Prunellae 14-15 part, Bulbus Allii 13-15 part, Fructus Sophorae 13-15 part, Fructus Gleditsia 13-15 part, Fructus Crataegi 12-14 part, Fructus Seu Herba Pubescentis 12-14 part, Cortex Hibisci 11-13 part, Mazus japonicus 10-12 part, bamboo salt 10-11 part, white vinegar 8-9 part, fish oil 9-11 part, dry red line worm powder 15-17 part, dry clam worm powder 15-17 part, Mel 6-8 part.

3. the feed additive of the red swordfish bacterial enteritis of prevention according to claim 1, it is characterized in that: the feed additive of the red swordfish bacterial enteritis of described prevention, be made up of each component of following masses number: Radix Rosae Normalis infusion 32 parts, Herba Hydrocotyles juice 19 parts, Herba Saxifragae 18 parts, Serpentis pimple 17 parts, Herba Cirsii juice 17 parts, Semen Myristicae 19 parts, Radix aconiti gymnandri 16 parts, the Radix Pulsatillae 16 parts, Herba Oxalidis Corniculatae 16 parts, Fructus Gardeniae 16 parts, Radix Gentianae 15 parts, Fructus Forsythiae 15 parts, Herba Wahlenbergiae 15 parts, Cortex Mori 15 parts, Fructus Cnidii 14 parts, Rhizoma Osmundae 14 parts, Galla Chinensis 15 parts, Spica Prunellae 14 parts, 14 parts, Bulbus Allii, the Fructus Sophorae 14 parts, 14 parts, Fructus Gleditsia, Fructus Crataegi 13 parts, Fructus Seu Herba Pubescentis 13 parts, Cortex Hibisci 12 parts, Mazus japonicus 11 parts, bamboo salt 11 parts, white vinegar 9 parts, 10 parts, fish oil, 16 parts dry, red line worm powder, dry clam worm powder 16 parts, Mel 7 parts.

4. a preparation method for the feed additive of the red swordfish bacterial enteritis of the prevention according to any one of claim 1-3, is characterized in that: comprise the following steps:

(1) take Radix Rosae Normalis by proportioning, add the purified water that parts by weight are 3 times of Radix Sanguisorbae parts by weight, at 70-80 DEG C, 12-24h is soaked in water-bath, filters to obtain Radix Rosae Normalis infusion;

(2) fresh Herba Hydrocotyles smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Hydrocotyles juice, takes Herba Hydrocotyles juice according to proportioning;

(3) Herba Cirsii smashed to pieces grinding or squeeze the juice with juice extractor, gained juice is Herba Cirsii juice, takes Herba Cirsii juice according to proportioning;

(4) take Herba Saxifragae and Serpentis pimple by proportioning, the gross weight of Herba Saxifragae and Serpentis pimple is denoted as 1 part, takes the purified water of 6 parts, at 70-80 DEG C, 2-3h is soaked in water-bath, obtains soak;

(5) boil 2h after the soak that step (4) obtains being heated to boiling, be separated and take out fried liquid, continue the purified water adding 3 parts in medical material, continue to boil 1.5h after being heated to boiling, merge twice fried liquid and be extracting solution;

(6) Semen Myristicae, Radix aconiti gymnandri, the Radix Pulsatillae, Herba Oxalidis Corniculatae, Fructus Gardeniae, Radix Gentianae, Fructus Forsythiae, Herba Wahlenbergiae, Cortex Mori, Fructus Cnidii, Rhizoma Osmundae, Galla Chinensis, Spica Prunellae, Bulbus Allii, the Fructus Sophorae, Fructus Gleditsia, Fructus Crataegi, Fructus Seu Herba Pubescentis, Cortex Hibisci and Mazus japonicus is taken respectively by said ratio, load ball mill grinding 2h, cross 80 mesh sieves, obtain grounds travel;

(7) grounds travel that extracting solution step (5) obtained and step (6) obtain is positioned in the extraction kettle in supercritical extraction instrument, carbon dioxide is utilized to extract as medium, extraction kettle pressure is 40Mpa, extraction temperature is 45 DEG C, separator pressure 12Mpa, separator temperature is 70 DEG C, extracts 2 hours, is extracted liquid;

(8) bamboo salt, white vinegar, fish oil, dry red line worm powder, dry clam worm powder and Mel is taken according to proportioning, mix homogeneously with the Radix Rosae Normalis infusion of step (1) gained, the Herba Hydrocotyles juice of step (2) gained, the Herba Cirsii juice of step (3) gained, the extract of step (7) gained, obtain object.

5. the preparation method of the feed additive of the red swordfish bacterial enteritis of prevention according to claim 4, it is characterized in that: the preparation method of the red line worm powder of described drying is: cleaned by red line worm fresh water, use distilled water flushing again three times, grind to form powdery after vacuum lyophilization to finish-drying, cross 80 mesh sieves.

6. the preparation method of the feed additive of the red swordfish bacterial enteritis of prevention according to claim 4, it is characterized in that: the preparation method of the clam worm powder of described drying is: cleaned by clam worm fresh water, use distilled water flushing again three times, grind to form powdery after vacuum lyophilization to finish-drying, cross 80 mesh sieves.

7. a using method for the feed additive of the red swordfish bacterial enteritis of the prevention according to any one of claim 1-3, is characterized in that: throw something and feed after being mixed homogeneously with the weight ratio of 1:30-1:10 with commercially available normal feedstuff by the feed additive of red for prevention of the present invention swordfish bacterial enteritis.

8. the using method of the feed additive of the red swordfish bacterial enteritis of prevention according to claim 7, is characterized in that: the feed additive of prevention of the present invention red swordfish bacterial enteritis and the weight ratio of commercially available normal feedstuff are 1:20.