CN104095081B - A kind of seed of Flos Moutan mixed oil of rich in vitamin E and preparation method thereof - Google Patents
A kind of seed of Flos Moutan mixed oil of rich in vitamin E and preparation method thereof Download PDFInfo
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- CN104095081B CN104095081B CN201310113026.2A CN201310113026A CN104095081B CN 104095081 B CN104095081 B CN 104095081B CN 201310113026 A CN201310113026 A CN 201310113026A CN 104095081 B CN104095081 B CN 104095081B
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Abstract
Seed of Flos Moutan mixed oil that the invention discloses a kind of rich in vitamin E and preparation method thereof, peony seed oil is prepared initially with microwave-assisted enzymatic isolation method, aqueous enzymatic method, cold-press is used to combine prepared Fructus Maydis oil, then vitamin E obtained by being concentrated with by extraction in soybean oil deodorizer distillate by two kinds of oil mix, uses the prepared edible oil nutrition of the method, health, stability strong.
Description
Technical field
The present invention relates to edible oil field, particularly relate to seed of Flos Moutan mixed oil and the preparation side thereof of a kind of rich in vitamin E
Method.
Background technology
Seed of Flos Moutan is the elite crystallization of peony plant, has passed on outside all characteristics that Paeonia suffruticosa itself has, and it more has oneself
Unique medicine and nutritional labeling, seed of Paeonia suffruticosa is by shell and the nut of kind shell double shielding, has natural " long-lived " base
Cause.Peony seed oil is plant oils and fats, for light yellow transparent liquid, has light Flos Moutan fragrance.Peony seed oil is unsaturated
Content of fatty acid reaches more than 90%, and especially estimable is wherein polyunsaturated fatty acid linolenic acid (belonging to w 3 series)
Content is more than 38%, and oleic acid content is more than 21%, and linoleic acid content is more than 25%.Linolenic acid, the linoleic acid cardiovascular and cerebrovascular vessel disease to people
Disease has well prevention and therapeutic efficiency；Additionally, linolenic acid also has antiallergic, absorption ultraviolet, delaying cell aging, prevents
The effects such as xerosis cutis.
Corn germ fatty content, between 17%～45%, constitutes about more than the 80% of Semen Maydis total fats content.Semen Maydis oil
In fatty acid feature be that unsaturated fatty acid content is up to 80%～85%.Semen Maydis oil itself does not contains cholesterol, and it is for blood
In liquid, the accumulation of cholesterol has dissolution, therefore can reduce and blood vessel is produced hardening effect.Hard to Senile disease such as tremulous pulse
Change, diabetes etc. have positive preventive and therapeutic effect.Due to the function of natural compound vitamin E, to heart disease, thrombotic vein
Inflammation, Reproductive Performance class obstacle, muscular atrophy, trophism encephalomalacia all have obvious curative effect and preventive effect.
Summary of the invention
The technical problem to be solved in the present invention is: provide the preparation method of the seed of Flos Moutan mixed oil of a kind of rich in vitamin E,
Mixed oil nutrition, health, stability that the method prepares are strong.
To achieve these goals, the technical solution used in the present invention is: the seed of Flos Moutan mixed oil of a kind of rich in vitamin E
Preparation method, comprise the following steps:
One, peony seed oil is prepared:
(1) choose full seed of Flos Moutan by wet cleaning process, dry, Integral-crushing, Kernel-hull separation, obtain seed of Flos Moutan core standby；
(2) the seed of Flos Moutan core that (1) obtains is baked 10～25min at 150～200 DEG C；
(3) the seed of Flos Moutan core baked being pulverized, being ground to granularity is 150～200 mesh, obtains seed of Flos Moutan slurry standby；
(4) uniformly being mixed with distilled water 1:3 in mass ratio～5 by seed of Flos Moutan slurry, adding concentration the most in the mixture is
The neutral protease liquid of 50mg/ml, liquid of protease accounts for the 0.05%～0.1% of mixture gross mass；
(5) material that (4) obtain is placed in Microwave Extraction Equipment, power be 700～900W, temperature 25～60 DEG C
Under the conditions of extract 10～20min；
(6) hydrochloric acid of the material 1mol/L (5) obtained adjusts pH to 4.5, then at 20 DEG C, 3500～5000 turns/min
Under conditions of centrifugal 10～15min, take supernatant liquid and obtain peony seed oil；
Two, Fructus Maydis oil is prepared:
(1) Fructus Maydis is crushed to 100～120 mesh, then with 100 ± 2 DEG C of baking oven for heating 4～6 minutes；
(2) will account for Fructus Maydis quality 1～4%, enzyme unit of living beThe compound enzyme of U/g and jade
Reconciling pH value after the distilled water mixing of Rice plumule quality 1% is 4～7, then is sprayed on the Fructus Maydis of (1) gained, and 50 ±
Enzymolysis 3～7 hours under the conditions of 2 DEG C, described compound enzyme is cellulase and a-amylase；
(3) heat 4～6 minutes under conditions of the Fructus Maydis that enzymolysis is crossed being put into 100 ± 2 DEG C；
(4) by (3) gained material 80 ± 2 DEG C, vacuum be to be dried under the conditions of 2000～3000Pa, be dried to material water
Dividing content is 5～10%；
(5) (4) gained material carrying out cold pressing: feeding temperature is 65～80 DEG C, pressure is 2.5～4MPa, the cold pressing time
It is 1～5 minute, obtains Fructus Maydis oil；
Three, mixed oil is prepared:
By peony seed oil with the Fructus Maydis oil prepared according to 1:5～8 ratio mix homogeneously, then according to 15～35mg/L
Adding vitamin E, this vitamin E is concentrated by extraction in soybean oil deodorizer distillate and obtains.
The seed of Flos Moutan mixed oil of a kind of rich in vitamin E prepared by said method, by peony seed oil, Fructus Maydis oil and
Vitamin E composition obtained by extraction concentration in soybean oil deodorizer distillate.
During the extraction of what the present invention brought have the beneficial effect that peony seed oil, with the addition of distilled water, with water as medium,
Realizing oil-water separation, the whole oil process that carries is equivalent to oil and have passed through the process of " washing ", eliminate impurity in peony seed oil with
Abnormal flavour.Use microwave-assisted enzymatic isolation method simultaneously, have that extraction rate is fast, grease extraction efficiency is high, solvent consumption is few, extraction conditions temperature
With etc. advantage, compared with simple microwave extraction, extraction ratio is increased to 65～87% by original 45～57%, and this technological operation letter
Oil quality single, that extract is good, low cost.
The extraction of Fructus Maydis oil uses aqueous enzymatic method to combine with cold-press.Compound enzyme selects cellulase and a-starch
Enzyme, when cellulase and the diastatic mixed proportion of a-be 4:3, compound enzyme consumption account for the 2.5% of Fructus Maydis quality, enzymolysis time
Between 7h, PH6.0 time, the extraction ratio of Fructus Maydis oil is more than 89%.After enzymolysis, use cold-press, phospholipid, pigment etc. be difficult to into
Enter in oil, it is to avoid the high temperature destruction to Oil stability, protect the nutritional labeling in natural flavour mountaineous, the color and luster of oil plant and oil
Such as phospholipid and natural Ve etc.；The course of processing is without the residual of chemical solvent；Oil after cold rolling is without refining process, it is to avoid and alkali
Liquid, phosphoric acid etc. contact, and prevent the harmful substance such as trans fatty acid, oils and fats polymer.
Soybean natural Ve is more suitable for adding to edible oil and fat than BHA and BHT, adds Ve and can extend the guarantor of mixed oil
Matter phase, minimizing Oxidation of Fat and Oils phenomenon.
Detailed description of the invention
Embodiment one
The preparation method of the seed of Flos Moutan mixed oil of a kind of rich in vitamin E, comprises the following steps:
One, peony seed oil is prepared:
(1) choose full seed of Flos Moutan by wet cleaning process, dry, Integral-crushing, Kernel-hull separation, obtain seed of Flos Moutan core standby；
(2) the seed of Flos Moutan core that (1) obtains is baked 10min at 150 DEG C；
(3) the seed of Flos Moutan core baked being pulverized, being ground to granularity is 150 mesh, obtains seed of Flos Moutan slurry standby；
(4) uniformly being mixed with distilled water 1:3 in mass ratio by seed of Flos Moutan slurry, adding concentration the most in the mixture is
The neutral protease liquid of 50mg/ml, liquid of protease accounts for the 0.05% of mixture gross mass；
(5) material that (4) obtain is placed in Microwave Extraction Equipment, is 700W at power, extracts under conditions of temperature 25 DEG C
Take 10min；
(6) hydrochloric acid of the material 1mol/L (5) obtained adjusts pH to 4.5, then 20 DEG C, the condition of 3500 turns/min
Under be centrifuged 10min, take supernatant liquid and obtain peony seed oil；
Two, Fructus Maydis oil is prepared:
(1) Fructus Maydis is crushed to 100 mesh, then by 100 ± 2 DEG C of baking oven for heating 4 minutes；
(2) will account for Fructus Maydis quality 1%, enzyme unit alive isThe compound enzyme of U/g and Fructus Maydis quality 1%
Distilled water mixing after to reconcile pH value be 4, then be sprayed on the Fructus Maydis of (1) gained, and enzymolysis 3 under the conditions of 50 ± 2 DEG C
Hour, described compound enzyme is cellulase and a-amylase；
(3) heat 4 minutes under conditions of the Fructus Maydis that enzymolysis is crossed being put into 100 ± 2 DEG C；
(4) by (3) gained material 80 ± 2 DEG C, vacuum be 2000Pa under the conditions of be dried, be dried to moisture content of material
It is 5%；
(5) (4) gained material carrying out cold pressing: feeding temperature is 65 DEG C, pressure is 2.5MPa, and the cold pressing time is 1 minute,
Obtain Fructus Maydis oil；
Three, mixed oil is prepared:
By peony seed oil with the Fructus Maydis oil prepared according to 1:5 ratio mix homogeneously, then according to 15mg/L adds dimension
Raw element E, this vitamin E is concentrated by extraction in soybean oil deodorizer distillate and obtains.
Edible oil and fat contain unsaturated fatty acid, owing to storage is improper or after high temperature fried food, often producing one is
The lipid peroxide of row.Lipid peroxide is a kind of harmful substance containing free radical, if accumulating in vivo, can destroy cellularity
And normal physiological function, promote generation and the development of disease, therefore the oils and fats of edible oxidation is extremely disadvantageous to the health of human body.For
Avoid Oxidation of Fat and Oils, general BHA and BHT adding chemosynthesis.But BHA and BHT is the most readily volatilized and loses it and resists
Oxidation, on the contrary, the soybean natural VE good fat-soluble antioxidant that is heat stability.
Embodiment two
The preparation method of the seed of Flos Moutan mixed oil of a kind of rich in vitamin E, comprises the following steps:
One, peony seed oil is prepared:
(1) choose full seed of Flos Moutan by wet cleaning process, dry, Integral-crushing, Kernel-hull separation, obtain seed of Flos Moutan core standby；
(2) the seed of Flos Moutan core that (1) obtains is baked 25min at 200 DEG C；
(3) the seed of Flos Moutan core baked being pulverized, being ground to granularity is 200 mesh, obtains seed of Flos Moutan slurry standby；
(4) uniformly being mixed with distilled water 1:5 in mass ratio by seed of Flos Moutan slurry, adding concentration the most in the mixture is
The neutral protease liquid of 50mg/ml, liquid of protease accounts for the 0.1% of mixture gross mass；
(5) material that (4) obtain is placed in Microwave Extraction Equipment, extracts under conditions of power is 900W, temperature 60 C
Take 20min；
(6) hydrochloric acid of the material 1mol/L (5) obtained adjusts pH to 4.5, then 20 DEG C, the condition of 5000 turns/min
Under be centrifuged 15min, take supernatant liquid and obtain peony seed oil；
Two, Fructus Maydis oil is prepared:
(1) Fructus Maydis is crushed to 120 mesh, then by 100 ± 2 DEG C of baking oven for heating 6 minutes；
(2) will account for Fructus Maydis quality 4%, enzyme unit alive isThe compound enzyme of U/g and Fructus Maydis quality 1%
Distilled water mixing after to reconcile pH value be 7, then be sprayed on the Fructus Maydis of (1) gained, and enzymolysis 7 under the conditions of 50 ± 2 DEG C
Hour, described compound enzyme is cellulase and a-amylase；
(3) heat 6 minutes under conditions of the Fructus Maydis that enzymolysis is crossed being put into 100 ± 2 DEG C；
(4) by (3) gained material 80 ± 2 DEG C, vacuum be 3000Pa under the conditions of be dried, be dried to moisture content of material
It is 10%；
(5) (4) gained material carrying out cold pressing: feeding temperature is 80 DEG C, pressure is 4MPa, and the cold pressing time is 5 minutes, i.e.
Obtain Fructus Maydis oil；
Three, mixed oil is prepared:
By peony seed oil with the Fructus Maydis oil prepared according to 1:8 ratio mix homogeneously, then according to 35mg/L adds dimension
Raw element E, this vitamin E is concentrated by extraction in soybean oil deodorizer distillate and obtains.
Embodiment three
The preparation method of the seed of Flos Moutan mixed oil of a kind of rich in vitamin E, comprises the following steps:
One, peony seed oil is prepared:
(1) choose full seed of Flos Moutan by wet cleaning process, dry, Integral-crushing, Kernel-hull separation, obtain seed of Flos Moutan core standby；
(2) the seed of Flos Moutan core that (1) obtains is baked 20min at 170 DEG C；
(3) the seed of Flos Moutan core baked being pulverized, being ground to granularity is 170 mesh, obtains seed of Flos Moutan slurry standby；
(4) uniformly being mixed with distilled water 1:4 in mass ratio by seed of Flos Moutan slurry, adding concentration the most in the mixture is
The neutral protease liquid of 50mg/ml, liquid of protease accounts for the 0.08% of mixture gross mass；
(5) material that (4) obtain is placed in Microwave Extraction Equipment, is 800W at power, extracts under conditions of temperature 40 DEG C
Take 15min；
(6) hydrochloric acid of the material 1mol/L (5) obtained adjusts pH to 4.5, then 20 DEG C, the condition of 4500 turns/min
Under be centrifuged 12min, take supernatant liquid and obtain peony seed oil；
Two, Fructus Maydis oil is prepared:
(1) Fructus Maydis is crushed to 110 mesh, then by 100 ± 2 DEG C of baking oven for heating 5 minutes；
(2) will account for Fructus Maydis quality 3%, enzyme unit alive isThe compound enzyme of U/g and Fructus Maydis quality 1%
Distilled water mixing after to reconcile pH value be 5, then be sprayed on the Fructus Maydis of (1) gained, and enzymolysis 5 under the conditions of 50 ± 2 DEG C
Hour, described compound enzyme is cellulase and a-amylase；
(3) heat 5 minutes under conditions of the Fructus Maydis that enzymolysis is crossed being put into 100 ± 2 DEG C；
(4) by (3) gained material 80 ± 2 DEG C, vacuum be 2500Pa under the conditions of be dried, be dried to moisture content of material
It is 8%；
(5) (4) gained material carrying out cold pressing: feeding temperature is 75 DEG C, pressure is 3.0MPa, and the cold pressing time is 4 minutes,
Obtain Fructus Maydis oil；
Three, mixed oil is prepared:
By peony seed oil with the Fructus Maydis oil prepared according to 1:7 ratio mix homogeneously, then according to 19mg/L adds dimension
Raw element E, this vitamin E is concentrated by extraction in soybean oil deodorizer distillate and obtains.When the addition of vitamin E is 19mg/L, with
About 150 DEG C deep-fried twisted dough sticks of temperature, compared with the edible oil adding BHA and BHT, no matter POV value or TBA value, are interpolation big
The natural V of beaneOil minimum.
Preparing in Fructus Maydis oil process (2nd) step, liquid ratio is 5:1mL/g, compound enzyme consumption 2.5%, enzymolysis time
When the diastatic mixed proportion of 7h, PH6.0, cellulase and a-is 4:3, the extraction ratio of Fructus Maydis oil is more than 89%.Water enzyme
Method uses compound enzyme gained oil extracting rate higher than single enzyme.This is because, oil point is primarily present in cytoplasmic network structure, only
Having and break away from the constraint of network structure in cell wall and matter, oil point could fully be discharged, and can obtain higher oil extracting rate accordingly.Cell
Wall be mainly composed of cellulose and hemicellulose and pectin, decomposition cell wall is played in the use of cellulase, the analysis divided for oil
Go out to open passage.In Fructus Maydis, content of starch is the highest, and when raw material carries out heat treatment, starch ripening, viscosity increases, a-amylase
Can starch-splitting, make oil point precipitation the easiest, make oil extracting rate raise.
Under the effect of enzyme, cell wall, the parcel lignin of oils and fats, cellulose etc. are degraded, thus isolate oils and fats.
Aqueous enzymatic method mild condition, technique are simple, it is not necessary to the PROCESS FOR TREATMENT such as degumming, deodorize, have that oil yield is high, oil product is good, do not destroy egg
The advantages such as white matter.
Claims (2)
1. the preparation method of the seed of Flos Moutan mixed oil of a rich in vitamin E, it is characterised in that comprise the following steps:
One, peony seed oil is prepared:
(1) choose full seed of Flos Moutan by wet cleaning process, dry, Integral-crushing, Kernel-hull separation, obtain seed of Flos Moutan core standby；
(2) the seed of Flos Moutan core that (1) obtains is baked 10～25min at 150～200 DEG C；
(3) the seed of Flos Moutan core baked being pulverized, being ground to granularity is 150～200 mesh, obtains seed of Flos Moutan slurry standby；
(4) uniformly being mixed with distilled water 1:3 in mass ratio～5 by seed of Flos Moutan slurry, adding concentration the most in the mixture is 50mg/
The neutral protease liquid of ml, liquid of protease accounts for the 0.05%～0.1% of mixture gross mass；
(5) material that (4) obtain is placed in Microwave Extraction Equipment, power be 700～900W, temperature 25～the condition of 60 DEG C
Lower extraction 10～20min；
(6) hydrochloric acid of the material 1mol/L (5) obtained adjusts pH to 4.5, then at 20 DEG C, the bar of 3500～5000 turns/min
It is centrifuged 10～15min under part, takes supernatant liquid and obtain peony seed oil；
Two, Fructus Maydis oil is prepared:
(1) Fructus Maydis is crushed to 100～120 mesh, then with 100 ± 2 DEG C of baking oven for heating 4～6 minutes；
(2) will account for Fructus Maydis quality 1～4%, enzyme unit of living beThe compound enzyme of U/g and corn germ
Reconciling pH value after the distilled water mixing of bud quality 1% is 4～7, then is sprayed on the Fructus Maydis of (1) gained, and at 50 ± 2 DEG C
Under the conditions of enzymolysis 3～7 hours, described compound enzyme is cellulase and a-amylase；
(3) heat 4～6 minutes under conditions of the Fructus Maydis that enzymolysis is crossed being put into 100 ± 2 DEG C；
(4) by (3) gained material 80 ± 2 DEG C, vacuum be to be dried under the conditions of 2000～3000Pa, be dried to material moisture and contain
Amount is 5～10%；
(5) (4) gained material carrying out cold pressing: feeding temperature is 65～80 DEG C, pressure is 2.5～4MPa, the cold pressing time be 1～
5 minutes, obtain Fructus Maydis oil；
Three, mixed oil is prepared:
By peony seed oil with the Fructus Maydis oil prepared according to 1:5～8 ratio mix homogeneously, then according to 15～35mg/L add
Vitamin E, this vitamin E is concentrated by extraction in soybean oil deodorizer distillate and obtains.
2. the seed of Flos Moutan mixed oil of a kind of rich in vitamin E prepared by the method described in claim 1, it is characterised in that by
Peony seed oil, Fructus Maydis oil and vitamin E composition obtained by extraction concentration in soybean oil deodorizer distillate.
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| CN106035757A (en) * | 2016-06-03 | 2016-10-26 | 河南洛阳红牡丹产业研发有限公司 | Preparation method of peony seed and okra seed oil |
| CN107410526A (en) * | 2017-05-17 | 2017-12-01 | 安徽华宇调味食品有限公司 | A kind of fragrance of a flower corn ready-mixed oil and its production technology |
| CN113122368B (en) * | 2019-12-31 | 2023-12-26 | 丰益(上海)生物技术研发中心有限公司 | Method for producing aroma by corn oil |
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|---|---|---|---|---|
| CN101099516A (en) * | 2007-07-25 | 2008-01-09 | 东北农业大学 | Method for extracting whole wheat-germ oil |
| CN101653174A (en) * | 2009-09-07 | 2010-02-24 | 兰州牡丹园艺开发公司 | Method for preparing blend oil and cold-pressing and extracting peony seed oil |
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