CN104095081A - Peony seed blend oil rich in vitamin E and preparation method thereof - Google Patents
Peony seed blend oil rich in vitamin E and preparation method thereof Download PDFInfo
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- CN104095081A CN104095081A CN201310113026.2A CN201310113026A CN104095081A CN 104095081 A CN104095081 A CN 104095081A CN 201310113026 A CN201310113026 A CN 201310113026A CN 104095081 A CN104095081 A CN 104095081A
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Abstract
The invention discloses a peony seed blend oil rich in vitamin E and a preparation method thereof. The method includes: firstly preparing peony seed oil by a microwave-assisted enzymolysis technique, combining an aqueous enzymatic technique and a cold pressing technique to prepare corn germ oil, and then mixing the two oil with vitamin E obtained by extraction and concentration of soybean oil deodorizer distillate. The edible oil prepared by the method is nutritious and healthy, and has strong stability.
Description
Technical field
The present invention relates to edible oil field, relate in particular to a kind of peony seeds ready-mixed oil that is rich in vitamin E and preparation method thereof.
Background technology
Peony seeds is the elite crystallization of peony plant, has passed on outside all characteristics that tree peony itself has, and it more has own unique medicine and nutritional labeling, and Paeonia suffruticosa seed is the nut that is subject to shell and plants shell double shielding, has natural " longevity " gene.Peony seed oil is plant grease, is light yellow transparent liquid, has light peony fragrance.Peony seed oil unsaturated fatty acid content reaches more than 90%, and especially estimable is that wherein polyunsaturated fatty acid---leukotrienes (belonging to w-3 series) content exceedes 38%, and oleic acid content is that more than 21% linoleic acid content is greater than 25%.Leukotrienes, linoleic acid have good prevention and therapeutic efficiency to people's cardiovascular and cerebrovascular disease; In addition, leukotrienes also has antiallergy, absorbs ultraviolet ray, delaying cell aging, prevents the effects such as dry skin.
Maize germ fat content, between 17%～45%, accounts for greatly the more than 80% of corn total fats content.Aliphatic acid feature in corn oil is that unsaturated fatty acid content is up to 80%～85%.Corn oil itself does not contain cholesterol, and it has dissolution for the accumulation of Blood Cholesterol, therefore can reduce, blood vessel is produced to hardening effect.The preventive and therapeutic effect as positive in artery sclerosis, diabetes etc. have to geriatric disease.Due to the function of natural B B-complex E, heart disease, thrombophlebitis, Reproductive Performance class obstacle, muscular atrophy, trophism encephalomalacia are all had to obvious curative effect and prevention effect.
Summary of the invention
The technical problem to be solved in the present invention is: a kind of preparation method of the peony seeds ready-mixed oil that is rich in vitamin E is provided, and ready-mixed oil nutrition, health, stability that the method makes are strong.
To achieve these goals, the technical solution used in the present invention is: a kind of preparation method of the peony seeds ready-mixed oil that is rich in vitamin E, comprises the following steps:
One, prepare peony seed oil:
(1) choose full peony seeds by wet cleaning process, dry, Integral-crushing, benevolence shell separates, and obtains peony seeds benevolence for subsequent use;
(2) the peony seeds benevolence (1) being obtained bakes 10～25min at 150～200 DEG C;
(3) the peony seeds benevolence baking being pulverized, is ground to granularity is 150～200 orders, obtains peony seeds slurry for subsequent use;
(4) peony seeds slurry is evenly mixed 1:3～5 in mass ratio with distilled water, then in mixture, adding concentration is the neutral proteinase liquid of 50mg/ml, and liquid of protease accounts for 0.05%～0.1% of mixture gross mass;
(5) material (4) being obtained is placed in Microwave Extraction Equipment, is to extract 10～20min under the condition of 25～60 DEG C of 700～900W, temperature at power;
(6) material (5) being obtained is adjusted pH to 4.5 with the hydrochloric acid of 1mol/L, and then centrifugal 10～15min under the condition of 20 DEG C, 3500～5000 turn/min, gets supernatant liquid and obtain peony seed oil;
Two, prepare maize germ oil:
(1) maize germ is crushed to 100～120 orders, then uses 100 ± 2 DEG C of baking oven heating 4～6 minutes;
(2) will account for maize germ quality 1～4%, Mei Huo unit is 0.8 × 10
5～1.2 × 10
5after the distilled water of the complex enzyme of U/g and maize germ quality 1% mixes, reconciling pH value is 4～7, then is sprayed on the maize germ of (1) gained, and under 50 ± 2 DEG C of conditions enzymolysis 3～7 hours, described complex enzyme is cellulase and a-amylase;
(3) maize germ of enzymolysis being crossed is put under the condition of 100 ± 2 DEG C and is heated 4～6 minutes;
(4) be dry under 2000～3000Pa condition by (3) gained material at 80 ± 2 DEG C, vacuum, being dried to moisture content of material is 5～10%;
(5) (4) gained material is carried out to cold press: feeding temperature is 65～80 DEG C, pressure is 2.5～4MPa, and the cold press time is 1～5 minute, obtains maize germ oil;
Three, prepare ready-mixed oil:
Peony seed oil and the maize germ oil making are mixed according to 1:5～8 ratio, then add vitamin E according to 15～35mg/L, this vitamin E is concentrated and is obtained by extraction in soybean oil deodorizer distillate.
A kind of peony seeds ready-mixed oil that is rich in vitamin E of being prepared by said method, by peony seed oil, maize germ oil and in soybean oil deodorizer distillate extraction concentrated and vitamin E form.
The beneficial effect that the present invention brings is: in the leaching process of peony seed oil, added distilled water, taking water as medium, realized water-oil separating, wholely put forward the process that oily process is equivalent to oil and has passed through " washing ", removed impurity and the peculiar smell in peony seed oil.Adopt microwave-assisted enzyme solution simultaneously, there is the advantages such as extraction rate is fast, grease recovery rate is high, solvent consumption is few, extraction conditions is gentle, compared with simple Microwave Extraction, recovery rate increases to 65～87% by original 45～57%, and this technological operation oil quality simple, that extract is good, cost is low.
The extraction of maize germ oil adopts aqueous enzymatic method to combine with cold-press.Complex enzyme is selected cellulase and a-amylase, when cellulase and the diastatic mixed proportion of a-be 4:3, complex enzyme consumption account for maize germ quality 2.5%, when enzymolysis time 7h, PH6.0, the recovery rate of maize germ oil is more than 89%.After enzymolysis, adopt cold-press, phosphatide, pigment etc. is difficult to enter in oil, has avoided the destruction of high temperature to Oil stability, has protected nutritional labeling in natural flavour mountaineous, color and luster and the oil of oil plant as phosphatide and natural Ve etc.; Process is residual without chemical solvent; Oil after cold rolling, without refining process, has been avoided contacting with alkali lye, phosphoric acid etc., prevents the harmful substance such as trans-fatty acid, grease condensate.
Soybean natural Ve is more suitable in being added into edible oil and fat than BHA and BHT, and interpolation Ve can extend shelf-life, the minimizing Oxidation of Fat and Oils phenomenon of ready-mixed oil.
Detailed description of the invention
Embodiment mono-
A preparation method who is rich in the peony seeds ready-mixed oil of vitamin E, comprises the following steps:
One, prepare peony seed oil:
(1) choose full peony seeds by wet cleaning process, dry, Integral-crushing, benevolence shell separates, and obtains peony seeds benevolence for subsequent use;
(2) the peony seeds benevolence (1) being obtained bakes 10min at 150 DEG C;
(3) the peony seeds benevolence baking being pulverized, is ground to granularity is 150 orders, obtains peony seeds slurry for subsequent use;
(4) by peony seeds slurry and distilled water in mass ratio 1:3 evenly mix, then in mixture, adding concentration is the neutral proteinase liquid of 50mg/ml, liquid of protease accounts for 0.05% of mixture gross mass;
(5) material (4) being obtained is placed in Microwave Extraction Equipment, is to extract 10min under the condition of 25 DEG C of 700W, temperature at power;
(6) material (5) being obtained is adjusted pH to 4.5 with the hydrochloric acid of 1mol/L, and then centrifugal 10min under the condition of 20 DEG C, 3500 turn/min, gets supernatant liquid and obtain peony seed oil;
Two, prepare maize germ oil:
(1) maize germ is crushed to 100 orders, then uses 100 ± 2 DEG C of baking oven heating 4 minutes;
(2) will account for maize germ quality 1%, Mei Huo unit is 0.8 × 10
5after the distilled water of the complex enzyme of U/g and maize germ quality 1% mixes, reconciling pH value is 4, then is sprayed on the maize germ of (1) gained, and under 50 ± 2 DEG C of conditions enzymolysis 3 hours, described complex enzyme is cellulase and a-amylase;
(3) maize germ of enzymolysis being crossed is put under the condition of 100 ± 2 DEG C and is heated 4 minutes;
(4) be dry under 2000Pa condition by (3) gained material at 80 ± 2 DEG C, vacuum, being dried to moisture content of material is 5%;
(5) (4) gained material is carried out to cold press: feeding temperature is 65 DEG C, pressure is 2.5MPa, and the cold press time is 1 minute, obtains maize germ oil;
Three, prepare ready-mixed oil:
Peony seed oil and the maize germ oil making are mixed according to 1:5 ratio, then add vitamin E according to 15mg/L, this vitamin E is concentrated and is obtained by extraction in soybean oil deodorizer distillate.
Edible oil and fat contain unrighted acid, owing to storing after improper or high temperature fried food, tend to produce a series of lipid peroxide.Lipid peroxide is a kind of harmful substance containing free radical, if accumulation in vivo can destroy eucaryotic cell structure and normal physiological function, impels generation and the development of disease, and therefore the grease of edible oxidation is extremely unfavorable to the health of human body.For avoiding Oxidation of Fat and Oils, generally add BHA and the BHT of chemical synthesis.But BHA and BHT at high temperature easily volatilize and lose its antioxidation, and on the contrary, soybean natural VE is the good fat-soluble antioxidant of heat endurance.
Embodiment bis-
A preparation method who is rich in the peony seeds ready-mixed oil of vitamin E, comprises the following steps:
One, prepare peony seed oil:
(1) choose full peony seeds by wet cleaning process, dry, Integral-crushing, benevolence shell separates, and obtains peony seeds benevolence for subsequent use;
(2) the peony seeds benevolence (1) being obtained bakes 25min at 200 DEG C;
(3) the peony seeds benevolence baking being pulverized, is ground to granularity is 200 orders, obtains peony seeds slurry for subsequent use;
(4) by peony seeds slurry and distilled water in mass ratio 1:5 evenly mix, then in mixture, adding concentration is the neutral proteinase liquid of 50mg/ml, liquid of protease accounts for 0.1% of mixture gross mass;
(5) material (4) being obtained is placed in Microwave Extraction Equipment, is to extract 20min under the condition of 900W, temperature 60 C at power;
(6) material (5) being obtained is adjusted pH to 4.5 with the hydrochloric acid of 1mol/L, and then centrifugal 15min under the condition of 20 DEG C, 5000 turn/min, gets supernatant liquid and obtain peony seed oil;
Two, prepare maize germ oil:
(1) maize germ is crushed to 120 orders, then uses 100 ± 2 DEG C of baking oven heating 6 minutes;
(2) will account for maize germ quality 4%, Mei Huo unit is 1.2 × 10
5after the distilled water of the complex enzyme of U/g and maize germ quality 1% mixes, reconciling pH value is 7, then is sprayed on the maize germ of (1) gained, and under 50 ± 2 DEG C of conditions enzymolysis 7 hours, described complex enzyme is cellulase and a-amylase;
(3) maize germ of enzymolysis being crossed is put under the condition of 100 ± 2 DEG C and is heated 6 minutes;
(4) be dry under 3000Pa condition by (3) gained material at 80 ± 2 DEG C, vacuum, being dried to moisture content of material is 10%;
(5) (4) gained material is carried out to cold press: feeding temperature is 80 DEG C, pressure is 4MPa, and the cold press time is 5 minutes, obtains maize germ oil;
Three, prepare ready-mixed oil:
Peony seed oil and the maize germ oil making are mixed according to 1:8 ratio, then add vitamin E according to 35mg/L, this vitamin E is concentrated and is obtained by extraction in soybean oil deodorizer distillate.
Embodiment tri-
A preparation method who is rich in the peony seeds ready-mixed oil of vitamin E, comprises the following steps:
One, prepare peony seed oil:
(1) choose full peony seeds by wet cleaning process, dry, Integral-crushing, benevolence shell separates, and obtains peony seeds benevolence for subsequent use;
(2) the peony seeds benevolence (1) being obtained bakes 20min at 170 DEG C;
(3) the peony seeds benevolence baking being pulverized, is ground to granularity is 170 orders, obtains peony seeds slurry for subsequent use;
(4) by peony seeds slurry and distilled water in mass ratio 1:4 evenly mix, then in mixture, adding concentration is the neutral proteinase liquid of 50mg/ml, liquid of protease accounts for 0.08% of mixture gross mass;
(5) material (4) being obtained is placed in Microwave Extraction Equipment, is to extract 15min under the condition of 40 DEG C of 800W, temperature at power;
(6) material (5) being obtained is adjusted pH to 4.5 with the hydrochloric acid of 1mol/L, and then centrifugal 12min under the condition of 20 DEG C, 4500 turn/min, gets supernatant liquid and obtain peony seed oil;
Two, prepare maize germ oil:
(1) maize germ is crushed to 110 orders, then uses 100 ± 2 DEG C of baking oven heating 5 minutes;
(2) will account for maize germ quality 3%, Mei Huo unit is 1.0 × 10
5after the distilled water of the complex enzyme of U/g and maize germ quality 1% mixes, reconciling pH value is 5, then is sprayed on the maize germ of (1) gained, and under 50 ± 2 DEG C of conditions enzymolysis 5 hours, described complex enzyme is cellulase and a-amylase;
(3) maize germ of enzymolysis being crossed is put under the condition of 100 ± 2 DEG C and is heated 5 minutes;
(4) be dry under 2500Pa condition by (3) gained material at 80 ± 2 DEG C, vacuum, being dried to moisture content of material is 8%;
(5) (4) gained material is carried out to cold press: feeding temperature is 75 DEG C, pressure is 3.0MPa, and the cold press time is 4 minutes, obtains maize germ oil;
Three, prepare ready-mixed oil:
Peony seed oil and the maize germ oil making are mixed according to 1:7 ratio, then add vitamin E according to 19mg/L, this vitamin E is concentrated and is obtained by extraction in soybean oil deodorizer distillate.When the addition of vitamin E is 19mg/L, with 150 DEG C of left and right deep-fried twisted dough sticks of temperature, compare with the edible oil that adds BHA and BHT, no matter POV value or TBA value are and add soybean natural V
eoil minimum.
Prepare in maize germ oil process (2) step, liquid ratio is 5:1mL/g, complex enzyme consumption 2.5%, enzymolysis time 7h, PH6.0, and when the diastatic mixed proportion of cellulase and a-is 4:3, the recovery rate of maize germ oil is more than 89%.Aqueous enzymatic method adopts complex enzyme gained oil extracting rate higher than single enzyme.This be because, oil content is mainly present in cytoplasmic network structure, only breaks away from the constraint of network structure in cell membrane and matter, oil content could fully be discharged, corresponding meeting obtains higher oil extracting rate.The main component of cell membrane is cellulose and hemicellulose and pectin, and decomposition cell membrane is played in the use of cellulase, for separating out of oil content opened passage.In maize germ, content of starch is very high, when raw material is heat-treated, and starch slaking, viscosity increases, and a-amylase can starch-splitting, makes separating out of oil content more easy, and oil extracting rate is raise.
Under the effect of enzyme, lignin, the cellulose etc. of cell membrane, parcel grease are degraded, thereby isolate grease.Aqueous enzymatic method mild condition, technique are simple, without coming unstuck, the PROCESS FOR TREATMENT such as deodorization, have that oil yield is high, oil product good, do not destroy the advantages such as protein.
Claims (2)
1. a preparation method who is rich in the peony seeds ready-mixed oil of vitamin E, is characterized in that, comprises the following steps:
One, prepare peony seed oil:
(1) choose full peony seeds by wet cleaning process, dry, Integral-crushing, benevolence shell separates, and obtains peony seeds benevolence for subsequent use;
(2) the peony seeds benevolence (1) being obtained bakes 10～25min at 150～200 DEG C;
(3) the peony seeds benevolence baking being pulverized, is ground to granularity is 150～200 orders, obtains peony seeds slurry for subsequent use;
(4) peony seeds slurry is evenly mixed 1:3～5 in mass ratio with distilled water, then in mixture, adding concentration is the neutral proteinase liquid of 50mg/ml, and liquid of protease accounts for 0.05%～0.1% of mixture gross mass;
(5) material (4) being obtained is placed in Microwave Extraction Equipment, is to extract 10～20min under the condition of 25～60 DEG C of 700～900W, temperature at power;
(6) material (5) being obtained is adjusted pH to 4.5 with the hydrochloric acid of 1mol/L, and then centrifugal 10～15min under the condition of 20 DEG C, 3500～5000 turn/min, gets supernatant liquid and obtain peony seed oil;
Two, prepare maize germ oil:
(1) maize germ is crushed to 100～120 orders, then uses 100 ± 2 DEG C of baking oven heating 4～6 minutes;
(2) will account for maize germ quality 1～4%, Mei Huo unit is 0.8 × 10
5～1.2 × 10
5after the distilled water of the complex enzyme of U/g and maize germ quality 1% mixes, reconciling pH value is 4～7, then is sprayed on the maize germ of (1) gained, and under 50 ± 2 DEG C of conditions enzymolysis 3～7 hours, described complex enzyme is cellulase and a-amylase;
(3) maize germ of enzymolysis being crossed is put under the condition of 100 ± 2 DEG C and is heated 4～6 minutes;
(4) be dry under 2000～3000Pa condition by (3) gained material at 80 ± 2 DEG C, vacuum, being dried to moisture content of material is 5～10%;
(5) (4) gained material is carried out to cold press: feeding temperature is 65～80 DEG C, pressure is 2.5～4MPa, and the cold press time is 1～5 minute, obtains maize germ oil;
Three, prepare ready-mixed oil:
Peony seed oil and the maize germ oil making are mixed according to 1:5～8 ratio, then add vitamin E according to 15～35mg/L, this vitamin E is concentrated and is obtained by extraction in soybean oil deodorizer distillate.
2. a kind of peony seeds ready-mixed oil that is rich in vitamin E of being prepared by method claimed in claim 1, is characterized in that, by peony seed oil, maize germ oil and in soybean oil deodorizer distillate extraction concentrated and vitamin E form.
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Cited By (3)
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| CN107410526A (en) * | 2017-05-17 | 2017-12-01 | 安徽华宇调味食品有限公司 | A kind of fragrance of a flower corn ready-mixed oil and its production technology |
| CN113122368A (en) * | 2019-12-31 | 2021-07-16 | 丰益(上海)生物技术研发中心有限公司 | Method for producing fragrance from corn oil |
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