CA2459718A1 - Polymerases d'acide nucleique de thermus scotoductus - Google Patents
Polymerases d'acide nucleique de thermus scotoductus Download PDFInfo
- Publication number
- CA2459718A1 CA2459718A1 CA002459718A CA2459718A CA2459718A1 CA 2459718 A1 CA2459718 A1 CA 2459718A1 CA 002459718 A CA002459718 A CA 002459718A CA 2459718 A CA2459718 A CA 2459718A CA 2459718 A1 CA2459718 A1 CA 2459718A1
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- CA
- Canada
- Prior art keywords
- leu
- glu
- ala
- arg
- val
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 238000000338 in vitro Methods 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000003999 initiator Substances 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 238000005304 joining Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 1
- 108010003700 lysyl aspartic acid Proteins 0.000 description 1
- 230000005389 magnetism Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 1
- 108010068488 methionylphenylalanine Proteins 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-O oxonium Chemical compound [OH3+] XLYOFNOQVPJJNP-UHFFFAOYSA-O 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 108010029020 prolylglycine Proteins 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 150000003212 purines Chemical class 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 230000000113 radiomimetic effect Effects 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007423 screening assay Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 125000001424 substituent group Chemical group 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 150000003573 thiols Chemical group 0.000 description 1
- 229940113082 thymine Drugs 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 229960000281 trometamol Drugs 0.000 description 1
- 238000009281 ultraviolet germicidal irradiation Methods 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- IBIDRSSEHFLGSD-UHFFFAOYSA-N valinyl-arginine Natural products CC(C)C(N)C(=O)NC(C(O)=O)CCCN=C(N)N IBIDRSSEHFLGSD-UHFFFAOYSA-N 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
L'invention concerne des acides nucléiques et des polypeptides destinés à une polymérase d'acide nucléique provenant d'une organisme thermophile, le Thermus scotoductus. L'invention concerne également des procédés d'utilisation desdits acides nucléiques et desdits polypeptides.
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US32221801P | 2001-09-14 | 2001-09-14 | |
| US60/322,218 | 2001-09-14 | ||
| US33448901P | 2001-11-30 | 2001-11-30 | |
| US60/334,489 | 2001-11-30 | ||
| PCT/US2002/029102 WO2003066804A2 (fr) | 2001-09-14 | 2002-09-13 | Polymerases d'acide nucleique de thermus scotoductus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2459718A1 true CA2459718A1 (fr) | 2003-08-14 |
Family
ID=27737292
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002459718A Abandoned CA2459718A1 (fr) | 2001-09-14 | 2002-09-13 | Polymerases d'acide nucleique de thermus scotoductus |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1466013A4 (fr) |
| JP (1) | JP2005516611A (fr) |
| AU (1) | AU2002366438A1 (fr) |
| CA (1) | CA2459718A1 (fr) |
| WO (1) | WO2003066804A2 (fr) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2208789B1 (fr) | 1997-03-12 | 2015-07-22 | Applied Biosystems, LLC | Polymérases ADN dotées de propriétées d'incorporation de nucléotide étiquetée améliorée |
| US20050042629A1 (en) | 2002-09-13 | 2005-02-24 | Applera Corporation | Thermus scotoductus nucleic acid polymerases |
| US7052877B2 (en) | 2001-11-30 | 2006-05-30 | Applera Corporation | Thermus brockianus nucleic acid polymerases |
| AU2004288017B2 (en) | 2003-11-03 | 2009-10-08 | United Kingdom Research And Innovation | Polymerase |
| JP4568571B2 (ja) * | 2004-10-06 | 2010-10-27 | ユニバーサル・バイオ・リサーチ株式会社 | 熱安定性又は熱活性ｄｎａポリメラーゼ及びそれをコードするｄｎａ |
| US20060292578A1 (en) * | 2005-06-28 | 2006-12-28 | Weidong Zheng | DNA polymerase blends and mutant DNA polymerases |
| WO2007076461A1 (fr) * | 2005-12-22 | 2007-07-05 | Ge Healthcare Bio-Sciences Corp. | Adn polymerase thermostable provenant de thermus scotoductus |
| WO2007076464A2 (fr) * | 2005-12-22 | 2007-07-05 | Ge Healthcare Bio-Sciences Corp. | Adn polymerase thermostable provenant de thermus filiformis |
| KR101660686B1 (ko) * | 2009-09-21 | 2016-09-28 | 유니벌시티 오브 더 프리 스테이트 | 우라늄（ｖｉ）을 환원시키는 단백질의 분리 |
| CA2831180C (fr) * | 2011-04-11 | 2017-02-14 | Keith Bauer | Adn polymerases avec une activite amelioree |
| EP2788481B1 (fr) | 2011-12-08 | 2018-03-07 | Roche Diagnostics GmbH | Adn polymérases ayant une activité améliorée |
| EP2788479B1 (fr) | 2011-12-08 | 2016-02-10 | Roche Diagnostics GmbH | Adn polymérases avec activité améliorée |
| JP6224613B2 (ja) | 2011-12-08 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6759226B1 (en) * | 2000-05-24 | 2004-07-06 | Third Wave Technologies, Inc. | Enzymes for the detection of specific nucleic acid sequences |
| US5541311A (en) * | 1992-12-07 | 1996-07-30 | Third Wave Technologies, Inc. | Nucleic acid encoding synthesis-deficient thermostable DNA polymerase |
| WO2001090337A2 (fr) * | 2000-05-24 | 2001-11-29 | Third Wave Technologies, Inc. | Detection d'arn |
-
2002
- 2002-09-13 EP EP02805994A patent/EP1466013A4/fr not_active Withdrawn
- 2002-09-13 AU AU2002366438A patent/AU2002366438A1/en not_active Abandoned
- 2002-09-13 JP JP2003566155A patent/JP2005516611A/ja not_active Withdrawn
- 2002-09-13 CA CA002459718A patent/CA2459718A1/fr not_active Abandoned
- 2002-09-13 WO PCT/US2002/029102 patent/WO2003066804A2/fr not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| AU2002366438A1 (en) | 2003-09-02 |
| EP1466013A4 (fr) | 2006-09-06 |
| WO2003066804A2 (fr) | 2003-08-14 |
| JP2005516611A (ja) | 2005-06-09 |
| WO2003066804A3 (fr) | 2004-08-12 |
| EP1466013A2 (fr) | 2004-10-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |