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BR9808368A - Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. - Google Patents

Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.

Info

Publication number
BR9808368A
BR9808368A BR9808368-6A BR9808368A BR9808368A BR 9808368 A BR9808368 A BR 9808368A BR 9808368 A BR9808368 A BR 9808368A BR 9808368 A BR9808368 A BR 9808368A
Authority
BR
Brazil
Prior art keywords
primers
interest
templates
polypeptide
library
Prior art date
Application number
BR9808368-6A
Other languages
English (en)
Inventor
Jesper Vind
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Publication of BR9808368A publication Critical patent/BR9808368A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

"PROCESSOS PARA A CONSTRUçãO DE UMA BIBLIOTECA DE POLINUCLEOTìDEOS, HOMóLOGOS RECOMBINADOS, PARA A IDENTIFICAçãO UM POLIPEPTìDEO DE INTERESSE, E, PARA A PRODUçãO DE UM POLIPEPTìDEO DE INTERESSE". Processo para a construção in vitro de uma biblioteca de polinucleotídeos homólogos recombinados a partir de um número de diferentes gabaritos de DNA de partida e iniciadores por deslocamentos de gabarito induzidos durante uma síntese de polinucleotídeos é descrito, assim: A. iniciadores estendidos são sintetizados por a) desnaturação de gabaritos de DNA, b) recombinação dos iniciadores para os gabaritos, c) extensão dos referidos iniciadores pelo uso de uma polimerase, d) parada da síntese, e e) separar os iniciadores estendidos dos gabaritos; B. um deslocamento de gabarito é induzido por a) isolamento dos iniciadores estendidos a partir dos gabaritos e repetindo as etapas A. b) a A.e) usando os iniciadores estendidos como tanto iniciadores com gabaritos, ou b) repetindo as etapas A.b) e A.e), C. este processo é terminado após um número apropriado de ciclos de etapas de processo A e B.a), A. e B.b), ou combinações dos mesmos. Opcionalmente, os polinucleotídeos são amplificados em uma reação PCR padrão com iniciadores específicos para seletivamente amplificar polinucleotídeos homólogos de interesse.
BR9808368-6A 1997-03-18 1998-03-18 Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. BR9808368A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK30797 1997-03-18
DK43497 1997-04-17
DK62597 1997-05-30
PCT/DK1998/000104 WO1998041653A1 (en) 1997-03-18 1998-03-18 An in vitro method for construction of a dna library

Publications (1)

Publication Number Publication Date
BR9808368A true BR9808368A (pt) 2000-05-23

Family

ID=27220576

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9808368-6A BR9808368A (pt) 1997-03-18 1998-03-18 Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse.

Country Status (14)

Country Link
EP (1) EP0973940B1 (pt)
JP (1) JP2001515356A (pt)
KR (1) KR20000076363A (pt)
CN (1) CN1249784A (pt)
AT (1) ATE399880T1 (pt)
AU (1) AU741834B2 (pt)
BR (1) BR9808368A (pt)
CA (1) CA2284253A1 (pt)
DE (1) DE69839668D1 (pt)
DK (1) DK0973940T3 (pt)
IL (1) IL131966A0 (pt)
NZ (1) NZ337900A (pt)
PL (1) PL335860A1 (pt)
WO (1) WO1998041653A1 (pt)

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AU741834B2 (en) 2001-12-13
WO1998041653A1 (en) 1998-09-24
CA2284253A1 (en) 1998-09-24
IL131966A0 (en) 2001-03-19
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DK0973940T3 (da) 2008-10-20
ATE399880T1 (de) 2008-07-15
CN1249784A (zh) 2000-04-05
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AU6611598A (en) 1998-10-12
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