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ATE510005T1 - Verfahren zur isolierung von zellen aus der nabelschnur - Google Patents

Verfahren zur isolierung von zellen aus der nabelschnur

Info

Publication number
ATE510005T1
ATE510005T1 AT03739908T AT03739908T ATE510005T1 AT E510005 T1 ATE510005 T1 AT E510005T1 AT 03739908 T AT03739908 T AT 03739908T AT 03739908 T AT03739908 T AT 03739908T AT E510005 T1 ATE510005 T1 AT E510005T1
Authority
AT
Austria
Prior art keywords
cells
cord
umbilical
umbile
isolation
Prior art date
Application number
AT03739908T
Other languages
English (en)
Inventor
Francois Auger
Lucie Germain
Murielle Remy-Zolghadri
Cindy Hayward
Original Assignee
Univ Laval
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Laval filed Critical Univ Laval
Application granted granted Critical
Publication of ATE510005T1 publication Critical patent/ATE510005T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0605Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0625Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629Keratinocytes; Whole skin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/069Vascular Endothelial cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/069Vascular Endothelial cells
    • C12N5/0691Vascular smooth muscle cells; 3D culture thereof, e.g. models of blood vessels
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Pregnancy & Childbirth (AREA)
  • Dermatology (AREA)
  • Rheumatology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT03739908T 2003-06-27 2003-06-27 Verfahren zur isolierung von zellen aus der nabelschnur ATE510005T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CA2003/000979 WO2005001081A1 (en) 2003-06-27 2003-06-27 Method of isolating cells from umbilical cord

Publications (1)

Publication Number Publication Date
ATE510005T1 true ATE510005T1 (de) 2011-06-15

Family

ID=33546112

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03739908T ATE510005T1 (de) 2003-06-27 2003-06-27 Verfahren zur isolierung von zellen aus der nabelschnur

Country Status (6)

Country Link
US (2) US20060199263A1 (de)
EP (1) EP1639099B1 (de)
AT (1) ATE510005T1 (de)
AU (1) AU2003304250A1 (de)
CA (1) CA2529718C (de)
WO (1) WO2005001081A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004210891B2 (en) * 2003-02-11 2008-09-25 Dolores Baksh Progenitor cells from Wharton's jelly of human umbilical cord
NZ553695A (en) 2004-08-16 2009-10-30 Cellres Corp Pte Ltd Isolation of stem/progenitor cells from amniotic membrane of umbilical cord
CN101479377A (zh) 2005-12-22 2009-07-08 简·恩尼斯 来自冷冻的脐带组织的活细胞
US9944900B2 (en) 2006-01-18 2018-04-17 Hemacell Perfusion Pulsatile perfusion extraction method for non-embryonic pluripotent stem cells
PT2029149T (pt) 2006-05-05 2017-08-23 Tissue Regeneration Therapeutics Inc Células progenitoras imunopriviligiadas e moduladoras
US20080064098A1 (en) * 2006-06-05 2008-03-13 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of maternal placental cells
US20080050814A1 (en) * 2006-06-05 2008-02-28 Cryo-Cell International, Inc. Procurement, isolation and cryopreservation of fetal placental cells
WO2008060037A1 (en) * 2006-11-15 2008-05-22 Seoul National University Industry Foundation Method for the simultaneous primary-isolation and expansion of endothelial stem/progenitor cell and mesenchymal stem cell derived from mammal including human umbilical cord
US8673547B2 (en) * 2007-03-08 2014-03-18 Hemacell Perfusion, Inc. Method for isolation of afterbirth derived cells
US7919318B2 (en) * 2007-06-13 2011-04-05 Chabio & Diostech Co., Ltd. Process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonc stem cells
KR20170005148A (ko) 2008-04-21 2017-01-11 티슈 리제너레이션 쎄라퓨틱스, 인코포레이티드 생물학적 또는 화학적 물질에 대한 예방 또는 치료를 위한 유전적으로 변형된 인간 탯줄 혈관주위 세포
KR101041914B1 (ko) * 2008-06-26 2011-06-15 부산대학교 산학협력단 셀레늄에 의해 탈분화된 세포, 이의 제조방법 및 이의 용도
EP2199383B1 (de) * 2008-12-22 2017-02-15 SpheroTec GmbH Verfahren zur Herstellung von Fibroblasten
CN102905710B (zh) * 2010-03-30 2015-03-18 海斯特赛尔有限公司 来自脐带胶质的新生物材料
US9677042B2 (en) 2010-10-08 2017-06-13 Terumo Bct, Inc. Customizable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system
EP3190186B1 (de) 2011-11-30 2023-12-13 Jörn Bullerdiek Expression von mirnas in plazentagewebe
US8940294B2 (en) 2012-03-02 2015-01-27 Tissuetech, Inc. Methods of isolating and culturing stem cells
BR102013021202B1 (pt) * 2013-08-20 2022-09-06 Ccb - Centro De Criogenia Brasil Ltda Processo de produção de células tronco multipotentes e progenitores
JP6612227B2 (ja) 2013-11-16 2019-11-27 テルモ ビーシーティー、インコーポレーテッド バイオリアクターにおける細胞増殖
JP6783143B2 (ja) 2014-03-25 2020-11-11 テルモ ビーシーティー、インコーポレーテッド 培地の受動的補充
WO2016049421A1 (en) 2014-09-26 2016-03-31 Terumo Bct, Inc. Scheduled feed
CN104894088A (zh) * 2015-05-29 2015-09-09 广州赛莱拉干细胞科技股份有限公司 消化酶组合物及其应用、脐带上皮细胞的分离培养方法
WO2017004592A1 (en) 2015-07-02 2017-01-05 Terumo Bct, Inc. Cell growth with mechanical stimuli
US11965175B2 (en) 2016-05-25 2024-04-23 Terumo Bct, Inc. Cell expansion
US11104874B2 (en) 2016-06-07 2021-08-31 Terumo Bct, Inc. Coating a bioreactor
US11685883B2 (en) 2016-06-07 2023-06-27 Terumo Bct, Inc. Methods and systems for coating a cell growth surface
US11624046B2 (en) 2017-03-31 2023-04-11 Terumo Bct, Inc. Cell expansion
CN110612344B (zh) 2017-03-31 2023-09-12 泰尔茂比司特公司 细胞扩增
JP2024511064A (ja) 2021-03-23 2024-03-12 テルモ ビーシーティー、インコーポレーテッド 細胞捕獲及び増殖
CN115322949B (zh) * 2022-07-26 2024-03-15 唐颐控股(深圳)有限公司 一种人脐静脉平滑肌细胞的分离培养方法及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5134065A (en) * 1986-05-16 1992-07-28 Monsanto Company Tissue plasminogen activator inhibitor and method of purification
US5919702A (en) 1996-10-23 1999-07-06 Advanced Tissue Science, Inc. Production of cartilage tissue using cells isolated from Wharton's jelly
US6537567B1 (en) 1997-07-03 2003-03-25 Massachusetts Institute Of Technology Tissue-engineered tubular construct having circumferentially oriented smooth muscle cells
WO1999054439A1 (en) 1998-04-17 1999-10-28 Science Research Laboratory, Inc. Isolation and purging of cells by means of osmotic pressure
JP2000139454A (ja) 1998-11-04 2000-05-23 Asahi Medical Co Ltd 細胞分離回収方法及び回収必要細胞含有液
WO2000073421A2 (en) * 1999-06-02 2000-12-07 Lifebank Services, L.L.C. Methods of isolation, cryopreservation, and therapeutic use of human amniotic epithelial cells
US20020045260A1 (en) * 2000-10-17 2002-04-18 Shih-Chieh Hung Method of isolating mesenchymal stem cells
US7560280B2 (en) 2000-11-03 2009-07-14 Kourion Therapeutics Gmbh Human cord blood derived unrestricted somatic stem cells (USSC)
MXPA03005014A (es) * 2000-12-06 2004-09-10 Robert J Hariri Metodo para recolectar celulas madres de la placenta.
US6995162B2 (en) 2001-01-12 2006-02-07 Amgen Inc. Substituted alkylamine derivatives and methods of use
IL158171A0 (en) 2001-04-10 2004-03-28 Bioergonomics Inc Compositions and methods for cell separation

Also Published As

Publication number Publication date
EP1639099B1 (de) 2011-05-18
US20060199263A1 (en) 2006-09-07
AU2003304250A1 (en) 2005-01-13
CA2529718C (en) 2012-10-23
CA2529718A1 (en) 2005-01-06
US7939323B2 (en) 2011-05-10
WO2005001081A1 (en) 2005-01-06
US20080102522A1 (en) 2008-05-01
EP1639099A1 (de) 2006-03-29

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