AR128824A1 - Métodos de análisis y cuantificación de variantes polipeptídicas de carga - Google Patents
Métodos de análisis y cuantificación de variantes polipeptídicas de cargaInfo
- Publication number
- AR128824A1 AR128824A1 ARP230100670A ARP230100670A AR128824A1 AR 128824 A1 AR128824 A1 AR 128824A1 AR P230100670 A ARP230100670 A AR P230100670A AR P230100670 A ARP230100670 A AR P230100670A AR 128824 A1 AR128824 A1 AR 128824A1
- Authority
- AR
- Argentina
- Prior art keywords
- charge variants
- quantification
- analysis
- methods
- isoelectric
- Prior art date
Links
- 238000004458 analytical method Methods 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 title 1
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 108090000765 processed proteins & peptides Proteins 0.000 title 1
- 238000011002 quantification Methods 0.000 title 1
- 239000012491 analyte Substances 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 238000001962 electrophoresis Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000012723 sample buffer Substances 0.000 abstract 2
- 238000011534 incubation Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44713—Particularly adapted electric power supply
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
- G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
- G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
- G01N2030/8831—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials involving peptides or proteins
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Engineering & Computer Science (AREA)
- Power Engineering (AREA)
- Peptides Or Proteins (AREA)
Abstract
Un método para cuantificar las variantes de carga dentro de un analito puede incluir la introducción de un buffer de muestra que comprende el analito en un capilar, la separación de las variantes de carga dentro del buffer de muestra a lo largo de un gradiente isoeléctrico, la incubación del capilar en un anticuerpo de detección, la cuantificación de una abundancia relativa de una variante de carga en base a una señal que corresponde al anticuerpo de detección. El método puede incluir además la generación de un electroferograma, en donde el electroferograma incluye un gráfico de una intensidad de una señal generada por una molécula reportera frente a un punto isoeléctrico a lo largo del gradiente isoeléctrico donde se detectó la señal.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202263269595P | 2022-03-18 | 2022-03-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR128824A1 true AR128824A1 (es) | 2024-06-19 |
Family
ID=86272286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP230100670A AR128824A1 (es) | 2022-03-18 | 2023-03-17 | Métodos de análisis y cuantificación de variantes polipeptídicas de carga |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20230296559A1 (es) |
| EP (1) | EP4493921A1 (es) |
| KR (1) | KR20240164561A (es) |
| CN (1) | CN119137473A (es) |
| AR (1) | AR128824A1 (es) |
| AU (1) | AU2023233590A1 (es) |
| IL (1) | IL315548A (es) |
| TW (1) | TW202346856A (es) |
| WO (1) | WO2023177836A1 (es) |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0535162T3 (da) * | 1990-06-21 | 1995-06-12 | Axis Biochemicals As | Analyse for analytvarianter |
| US7087411B2 (en) | 1999-06-08 | 2006-08-08 | Regeneron Pharmaceuticals, Inc. | Fusion protein capable of binding VEGF |
| SG106672A1 (en) | 2002-03-08 | 2004-10-29 | Asml Netherlands Bv | Mask for use in lithography, method of making a mask, lithographic apparatus, and device manufacturing method |
| BRPI0712224B8 (pt) | 2006-06-02 | 2021-05-25 | Regeneron Pharma | anticorpos de alta afinidade para o receptor de il-6 humano e composições farmacêuticas |
| US7608693B2 (en) | 2006-10-02 | 2009-10-27 | Regeneron Pharmaceuticals, Inc. | High affinity human antibodies to human IL-4 receptor |
| KR101561020B1 (ko) | 2007-07-31 | 2015-10-26 | 리제너론 파아마슈티컬스, 인크. | 사람 ｃｄ20에 대한 사람 항체 및 이의 사용 방법 |
| JO3672B1 (ar) | 2008-12-15 | 2020-08-27 | Regeneron Pharma | أجسام مضادة بشرية عالية التفاعل الكيماوي بالنسبة لإنزيم سبتيليسين كنفرتيز بروبروتين / كيكسين نوع 9 (pcsk9). |
| JO3417B1 (ar) | 2010-01-08 | 2019-10-20 | Regeneron Pharma | الصيغ المستقرة التي تحتوي على الأجسام المضادة لمضاد مستقبل( interleukin-6 (il-6r |
| JO3340B1 (ar) | 2010-05-26 | 2019-03-13 | Regeneron Pharma | مضادات حيوية لـعامل تمايز النمو 8 البشري |
| AR083044A1 (es) | 2010-09-27 | 2013-01-30 | Regeneron Pharma | Anticuerpos anti-cd48 y usos de los mismos |
| EP2624865B1 (en) | 2010-10-06 | 2018-08-01 | Regeneron Pharmaceuticals, Inc. | Stabilized formulations containing anti-interleukin-4 receptor (il-4r) antibodies |
| JO3756B1 (ar) | 2010-11-23 | 2021-01-31 | Regeneron Pharma | اجسام مضادة بشرية لمستقبلات الجلوكاجون |
| JO3412B1 (ar) | 2011-06-17 | 2019-10-20 | Regeneron Pharma | أجسام مضادة ل angptl3 واستخداماتها |
| US8871209B2 (en) | 2011-11-14 | 2014-10-28 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for increasing muscle mass and muscle strength by specifically antagonizing GDF8 and or Activin A |
| KR102063028B1 (ko) | 2012-01-23 | 2020-01-07 | 리제너론 파아마슈티컬스, 인크. | 항-ａｎｇ2 항체를 함유하는 안정화된 제형 |
| JO3820B1 (ar) | 2012-05-03 | 2021-01-31 | Regeneron Pharma | أجسام مضادة بشرية لـ fel d1وطرق لاستخدامها |
| TW201843172A (zh) | 2012-06-25 | 2018-12-16 | 美商再生元醫藥公司 | 抗－ｅｇｆｒ抗體及其用途 |
| MX363213B (es) | 2012-08-13 | 2019-03-15 | Regeneron Pharma | Anticuerpos anti-pcsk9 con características de unión dependientes del ph. |
| JOP20200236A1 (ar) | 2012-09-21 | 2017-06-16 | Regeneron Pharma | الأجسام المضادة لمضاد cd3 وجزيئات ربط الأنتيجين ثنائية التحديد التي تربط cd3 وcd20 واستخداماتها |
| JO3405B1 (ar) | 2013-01-09 | 2019-10-20 | Regeneron Pharma | الأجسام المضادة لمضاد مستقبل عامل النمو المشتق من الصفائح الدموية - بيتا واستخداماتها |
| JO3532B1 (ar) | 2013-03-13 | 2020-07-05 | Regeneron Pharma | الأجسام المضادة لمضاد انترلوكين-33 واستعمالاتها |
| TWI659968B (zh) | 2013-03-14 | 2019-05-21 | 再生元醫藥公司 | 針對呼吸道融合病毒ｆ蛋白質的人類抗體及其使用方法 |
| WO2014152195A1 (en) | 2013-03-15 | 2014-09-25 | Regeneron Pharmaceuticals, Inc. | Il-33 antagonists and uses thereof |
| TWI641620B (zh) | 2013-08-21 | 2018-11-21 | 再生元醫藥公司 | 抗－ｐｒｌｒ抗體及其用途 |
| TWI681969B (zh) | 2014-01-23 | 2020-01-11 | 美商再生元醫藥公司 | 針對ｐｄ-１的人類抗體 |
| TWI680138B (zh) | 2014-01-23 | 2019-12-21 | 美商再生元醫藥公司 | 抗ｐｄ-ｌ１之人類抗體 |
| SI3126388T1 (sl) | 2014-03-11 | 2019-08-30 | Regeneron Pharmaceuticals, Inc. | Protitelesa ANTI-EGFRVIII in njihova uporaba |
| TWI754319B (zh) | 2014-03-19 | 2022-02-01 | 美商再生元醫藥公司 | 用於腫瘤治療之方法及抗體組成物 |
| RU2713327C2 (ru) | 2014-05-05 | 2020-02-04 | Регенерон Фармасьютикалз, Инк. | Гуманизированные животные по с5 и с3 |
| WO2016044337A1 (en) | 2014-09-16 | 2016-03-24 | Regeneron Pharmaceuticals, Inc. | Anti-glucagon antibodies and uses thereof |
| JP7399845B2 (ja) * | 2017-08-18 | 2023-12-18 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 試料マトリックスにおいてタンパク質変異体を分析するための画像キャピラリー等電点電気泳動法 |
| US11782023B2 (en) * | 2018-12-19 | 2023-10-10 | Regeneron Pharmaceuticals, Inc. | Ce-western applications for antibody development |
| JP2022519246A (ja) * | 2019-01-31 | 2022-03-22 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 抗体電荷不均一性についてのネイティブマイクロ流体ｃｅ－ｍｓ解析法 |
| EP3973297A1 (en) * | 2019-05-23 | 2022-03-30 | Regeneron Pharmaceuticals, Inc. | Characterization of domain-specific charge variants of antibodies |
-
2023
- 2023-03-17 CN CN202380038645.1A patent/CN119137473A/zh active Pending
- 2023-03-17 KR KR1020247034590A patent/KR20240164561A/ko unknown
- 2023-03-17 EP EP23720398.9A patent/EP4493921A1/en active Pending
- 2023-03-17 TW TW112110091A patent/TW202346856A/zh unknown
- 2023-03-17 WO PCT/US2023/015446 patent/WO2023177836A1/en active Application Filing
- 2023-03-17 US US18/185,624 patent/US20230296559A1/en active Pending
- 2023-03-17 AU AU2023233590A patent/AU2023233590A1/en active Pending
- 2023-03-17 AR ARP230100670A patent/AR128824A1/es unknown
- 2023-03-17 IL IL315548A patent/IL315548A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AU2023233590A1 (en) | 2024-10-17 |
| EP4493921A1 (en) | 2025-01-22 |
| CN119137473A (zh) | 2024-12-13 |
| US20230296559A1 (en) | 2023-09-21 |
| IL315548A (en) | 2024-11-01 |
| WO2023177836A1 (en) | 2023-09-21 |
| KR20240164561A (ko) | 2024-11-19 |
| TW202346856A (zh) | 2023-12-01 |
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