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NO20072507L - Reagenser og fremgangsmater for a bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon - Google Patents

Reagenser og fremgangsmater for a bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon

Info

Publication number
NO20072507L
NO20072507L NO20072507A NO20072507A NO20072507L NO 20072507 L NO20072507 L NO 20072507L NO 20072507 A NO20072507 A NO 20072507A NO 20072507 A NO20072507 A NO 20072507A NO 20072507 L NO20072507 L NO 20072507L
Authority
NO
Norway
Prior art keywords
priming
reagents
pcr amplification
methods
pcr
Prior art date
Application number
NO20072507A
Other languages
English (en)
Other versions
NO341716B1 (no
Inventor
Lawrence J Wangh
John Rice
Aquiles J Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Original Assignee
Univ Brandeis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Brandeis filed Critical Univ Brandeis
Publication of NO20072507L publication Critical patent/NO20072507L/no
Publication of NO341716B1 publication Critical patent/NO341716B1/no

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Et tilsetningsstoff for forhindring av feilpriming i polymerasekjedereaksjon (PCR)-amplifiseringer og -analyser som omfatter et hårnålsoligonukleotid med en stammedupleks som er lengre enn seks nukleotider og en stabilisert stammeende. Tilsetningsstoffet forbedrer PCR-amplifiseringer, innbefattet LATE-amplifiseringer, når det tilsettes til de innledende amplifiseringsreaksjons-blandingene. Det kan inngå i oligonukleotidsett og i sett for PCR-amplifisering og -analyser.
NO20072507A 2004-10-18 2007-05-15 Reagenser og fremgangsmåter for å bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon NO341716B1 (no)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (2)

Publication Number Publication Date
NO20072507L true NO20072507L (no) 2007-07-06
NO341716B1 NO341716B1 (no) 2018-01-08

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
NO20072507A NO341716B1 (no) 2004-10-18 2007-05-15 Reagenser og fremgangsmåter for å bedre reproduserbarhet og redusere feil priming i PCR-amplifikasjon

Country Status (14)

Country Link
US (2) US7517977B2 (no)
EP (1) EP1802774B1 (no)
JP (2) JP5165376B2 (no)
KR (1) KR20070090158A (no)
CN (1) CN101076608B (no)
AT (1) ATE541055T1 (no)
AU (1) AU2005295299B2 (no)
BR (1) BRPI0516619B1 (no)
CA (1) CA2584576C (no)
ES (1) ES2380775T3 (no)
NO (1) NO341716B1 (no)
NZ (1) NZ554701A (no)
RU (1) RU2495046C2 (no)
WO (1) WO2006044995A1 (no)

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CN101321877B (zh) * 2005-10-03 2013-04-10 应用生物系统有限责任公司 用于扩增核酸的组合物、方法和试剂盒
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
US9034605B2 (en) * 2009-03-12 2015-05-19 Brandeis University Reagents and methods for PCR
DK2412718T3 (en) * 2009-03-26 2017-01-30 Xiamen Amoy Diagnostics Co Ltd CIRCUIT PRIMES USED BY NUCLEIC ACID AMPLIFICATION AND USE THEREOF
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
ES2743959T3 (es) 2009-10-21 2020-02-21 Univ Brandeis Kits para analizar secuencias de ácido nucleico monocatenario
EP2646576B1 (en) 2010-12-03 2015-10-28 Brandeis University Methods and kits for detecting nucleic acid mutants in wild-type populations
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
CN105164274B (zh) 2013-01-23 2018-11-30 布兰代斯大学 用于提高pcr准确度的试剂
WO2015013521A1 (en) * 2013-07-24 2015-01-29 Brandeis University Dna methylation analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
EP3146080B1 (en) * 2014-05-19 2021-01-06 William Marsh Rice University Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
US9477198B2 (en) 2014-09-03 2016-10-25 Samsung Electronics Co., Ltd. Cleaning device and electrophotographic image forming apparatus having the same
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WO2016100335A1 (en) * 2014-12-19 2016-06-23 Brandeis University Mispriming prevention reagents
US10557134B2 (en) 2015-02-24 2020-02-11 Trustees Of Boston University Protection of barcodes during DNA amplification using molecular hairpins
WO2017201239A1 (en) * 2016-05-18 2017-11-23 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
MX2019003273A (es) 2016-09-23 2019-09-13 Alveo Tech Inc Metodos y composiciones para detectar analitos.
EP3899023A4 (en) * 2018-12-20 2022-10-12 Alveo Technologies Inc. METHODS AND COMPOSITIONS FOR REDUCING NON-SPECIFIC AMPLIFICATION IN ISOTHERMAL AMPLIFICATION REACTIONS
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트

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Also Published As

Publication number Publication date
JP2008516613A (ja) 2008-05-22
AU2005295299B2 (en) 2010-10-21
BRPI0516619B1 (pt) 2016-02-10
WO2006044995A1 (en) 2006-04-27
CA2584576C (en) 2015-12-08
EP1802774B1 (en) 2012-01-11
JP2013063070A (ja) 2013-04-11
RU2495046C2 (ru) 2013-10-10
RU2007118546A (ru) 2008-11-27
US7517977B2 (en) 2009-04-14
ATE541055T1 (de) 2012-01-15
US20090226973A1 (en) 2009-09-10
JP5165376B2 (ja) 2013-03-21
ES2380775T3 (es) 2012-05-18
BRPI0516619A (pt) 2008-09-16
AU2005295299A1 (en) 2006-04-27
EP1802774A4 (en) 2008-10-01
CN101076608A (zh) 2007-11-21
KR20070090158A (ko) 2007-09-05
US20060177842A1 (en) 2006-08-10
NZ554701A (en) 2010-03-26
NO341716B1 (no) 2018-01-08
CA2584576A1 (en) 2006-04-27
EP1802774A1 (en) 2007-07-04
CN101076608B (zh) 2013-04-17

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