ATE550441T1 - Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren - Google Patents
Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäurenInfo
- Publication number
- ATE550441T1 ATE550441T1 AT06803026T AT06803026T ATE550441T1 AT E550441 T1 ATE550441 T1 AT E550441T1 AT 06803026 T AT06803026 T AT 06803026T AT 06803026 T AT06803026 T AT 06803026T AT E550441 T1 ATE550441 T1 AT E550441T1
- Authority
- AT
- Austria
- Prior art keywords
- compositions
- methods
- kits
- nucleic acids
- isothermal amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US71428105P | 2005-09-06 | 2005-09-06 | |
US72202805P | 2005-09-29 | 2005-09-29 | |
PCT/US2006/034663 WO2007030505A1 (en) | 2005-09-06 | 2006-09-06 | Methods, compositions and kits for isothermal amplification of nucleic acids |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE550441T1 true ATE550441T1 (de) | 2012-04-15 |
Family
ID=37607248
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT06803026T ATE550441T1 (de) | 2005-09-06 | 2006-09-06 | Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren |
Country Status (7)
Country | Link |
---|---|
US (1) | US20070054301A1 (de) |
EP (1) | EP1929045B9 (de) |
JP (1) | JP2009506788A (de) |
AT (1) | ATE550441T1 (de) |
AU (1) | AU2006287548A1 (de) |
CA (1) | CA2621146A1 (de) |
WO (1) | WO2007030505A1 (de) |
Families Citing this family (44)
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US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
EP1356094B1 (de) * | 2000-06-26 | 2010-01-13 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren |
ATE475720T1 (de) | 2000-12-13 | 2010-08-15 | Nugen Technologies Inc | Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben |
US8507662B2 (en) | 2001-01-19 | 2013-08-13 | General Electric Company | Methods and kits for reducing non-specific nucleic acid amplification |
EP1366197B1 (de) | 2001-03-09 | 2007-05-09 | Nugen Technologies, Inc. | Methoden und zusammensetzungen zur vervielfältigung von rna sequenzen |
EP1613778B1 (de) | 2003-04-14 | 2014-11-26 | Nugen Technologies, Inc. | Globale amplifizierung unter verwendung von zufallsgeprimeten zusammen gesetzten primern |
US7939258B2 (en) * | 2005-09-07 | 2011-05-10 | Nugen Technologies, Inc. | Nucleic acid amplification procedure using RNA and DNA composite primers |
US20090047673A1 (en) | 2006-08-22 | 2009-02-19 | Cary Robert B | Miniaturized lateral flow device for rapid and sensitive detection of proteins or nucleic acids |
US7794985B2 (en) * | 2007-04-04 | 2010-09-14 | Ghc Technologies, Inc. | Methods and compositions for rapid amplification, capture and detection of nucleic acids and proteins |
US8034568B2 (en) * | 2008-02-12 | 2011-10-11 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
WO2009102896A2 (en) * | 2008-02-12 | 2009-08-20 | Nugen Technologies, Inc. | Isothermal nucleic acid amplification methods and compositions |
US7846666B2 (en) * | 2008-03-21 | 2010-12-07 | Nugen Technologies, Inc. | Methods of RNA amplification in the presence of DNA |
CN102084238B (zh) | 2008-05-05 | 2016-06-01 | 洛斯阿拉莫斯国家安全有限责任公司 | 基于高度简化的侧向流动的核酸样品制备和被动流体流动控制 |
KR20100019220A (ko) * | 2008-08-08 | 2010-02-18 | 삼성전자주식회사 | 열순환을 포함하는 다중 치환 증폭에 의하여 표적 핵산 서열을 증폭하는 방법 |
US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
US20110104686A1 (en) * | 2009-10-29 | 2011-05-05 | Bio-Rad Laboratories, Inc. | Rapid detection of mycoplasma contamination in cell culture samples |
EP2369325A1 (de) | 2010-03-12 | 2011-09-28 | Eppendorf Ag | Arrayanalyse für Online-Detektion |
EP2625526B1 (de) | 2010-10-04 | 2017-03-15 | Genapsys Inc. | Systeme und verfahren für automatisierte und wiederverwendbare parallele biologische reaktionen |
EP2652148B1 (de) * | 2010-12-17 | 2016-11-30 | Life Technologies Corporation | Verfahren, zusammensetzungen, systeme, vorrichtungen und kits für nukleinsäureamplifikation |
GB201101488D0 (en) * | 2011-01-28 | 2011-03-16 | Ge Healthcare Ltd | Container storing freeze dried biological sample |
ES2621390T5 (es) | 2011-04-20 | 2020-10-06 | Mesa Biotech Inc | Reacción de amplificación oscilante para ácidos nucleicos |
US9926596B2 (en) | 2011-05-27 | 2018-03-27 | Genapsys, Inc. | Systems and methods for genetic and biological analysis |
CN104105797B (zh) | 2011-12-01 | 2016-08-31 | 吉纳普赛斯股份有限公司 | 用于高效电子测序与检测的系统和方法 |
US10975423B2 (en) * | 2013-03-11 | 2021-04-13 | Elitechgroup, Inc. | Methods for true isothermal strand displacement amplification |
US10590474B2 (en) * | 2013-03-11 | 2020-03-17 | Elitechgroup B.V. | Methods for true isothermal strand displacement amplification |
JP2016510991A (ja) * | 2013-03-11 | 2016-04-14 | エリテックグループ・ベスローテン・フェンノートシャップElitechgroup B.V. | 正確な鎖置換型等温増幅のための方法 |
WO2014158628A1 (en) | 2013-03-14 | 2014-10-02 | Hologic, Inc. | Compositions and methods for analysis of nucleic acid molecules |
US10450595B2 (en) | 2013-03-15 | 2019-10-22 | Theranos Ip Company, Llc | Nucleic acid amplification |
EP2971141B1 (de) | 2013-03-15 | 2018-11-28 | Genapsys, Inc. | Systeme für biologische analysen |
KR20150132481A (ko) | 2013-03-15 | 2015-11-25 | 테라노스, 인코포레이티드 | 핵산 증폭 |
CA2906824C (en) | 2013-03-15 | 2023-10-03 | Theranos, Inc. | Nucleic acid amplification |
EP2970960B1 (de) | 2013-03-15 | 2019-03-13 | Theranos IP Company, LLC | Nukleinsäureamplifikation |
EP3042208A4 (de) | 2013-09-06 | 2017-04-19 | Theranos, Inc. | Systeme und verfahren zur erkennung von infektionskrankheiten |
EP3080300B1 (de) | 2013-12-11 | 2020-09-02 | Genapsys Inc. | Systeme und verfahren für biologische analysen und berechnungen |
US9850525B2 (en) | 2014-01-29 | 2017-12-26 | Agilent Technologies, Inc. | CAS9-based isothermal method of detection of specific DNA sequence |
WO2015161054A2 (en) | 2014-04-18 | 2015-10-22 | Genapsys, Inc. | Methods and systems for nucleic acid amplification |
EP4282532A3 (de) | 2015-04-24 | 2024-02-28 | Mesa Biotech, Inc. | Fluidische testkassette |
EP3488017A4 (de) | 2016-07-20 | 2020-02-26 | Genapsys Inc. | Systeme und verfahren zur nukleinsäuresequenzierung |
SG11202002516WA (en) | 2017-09-21 | 2020-04-29 | Genapsys Inc | Systems and methods for nucleic acid sequencing |
EP3530755B1 (de) * | 2018-02-26 | 2020-08-26 | AGCT GmbH | Verfahren zum anzeigen des fortschrittes der amplifikation von nukleinsäuren und kit zu dessen durchführung |
GB201804585D0 (en) * | 2018-03-22 | 2018-05-09 | Dnae Diagnostics Ltd | Methods for amplication of nucleic acids with Endonuclease-Mediated shifting equilibrium amplification (EM-SEq) |
WO2020092268A2 (en) * | 2018-10-29 | 2020-05-07 | Kutyavin Igor V | Thermokinetically balanced isothermal amplification of nucleic acid sequences |
CN112029905B (zh) * | 2020-08-18 | 2023-08-11 | 珠海赛乐奇生物技术股份有限公司 | 一种检测torch病原体的基因芯片和试剂盒 |
IL307172A (en) | 2021-03-30 | 2023-11-01 | Illumina Inc | Improved methods of isothermal complementary DNA and library preparation |
Family Cites Families (44)
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US4683196A (en) * | 1983-12-12 | 1987-07-28 | Meru, Inc. | Method and materials for the identification of lipopolysaccharide producing microorganisms |
US4786600A (en) * | 1984-05-25 | 1988-11-22 | The Trustees Of Columbia University In The City Of New York | Autocatalytic replication of recombinant RNA |
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
WO1989001050A1 (en) * | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
US5124246A (en) * | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
CA1339731C (en) * | 1988-10-12 | 1998-03-17 | Charles T. Caskey | Multiplex genomic dna amplification for deletion detection |
CA2020958C (en) * | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
US5516663A (en) * | 1990-01-26 | 1996-05-14 | Abbott Laboratories | Ligase chain reaction with endonuclease IV correction and contamination control |
US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
DK0549107T3 (da) * | 1991-10-11 | 1998-08-31 | Dade Behring Marburg Gmbh | Fremgangsmåde til fremstilling af et polynukleotidtil anvendelse ved enkeltprimeramplifikation og phosphorthioatholdige oli |
US5714320A (en) * | 1993-04-15 | 1998-02-03 | University Of Rochester | Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides |
US5422252A (en) * | 1993-06-04 | 1995-06-06 | Becton, Dickinson And Company | Simultaneous amplification of multiple targets |
ES2114692T3 (es) * | 1993-06-09 | 1998-06-01 | Gamera Bioscience Corp | Reaccion en ciclos magneticos. |
JPH09506762A (ja) * | 1993-08-18 | 1997-07-08 | アイディー バイオメディカル コーポレイション | 近接配列−酵素分子を利用して標的核酸配列を検出するための組成物および方法 |
US5834252A (en) * | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
KR100230718B1 (ko) * | 1994-03-16 | 1999-11-15 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 등온 가닥 변위 핵산 증폭법 |
US6100078A (en) * | 1994-04-01 | 2000-08-08 | Gen-Probe Incorporated | Purified DNA polymerase from bacillus stearothermophilus ATCC 12980 |
FR2721945B1 (fr) | 1994-07-04 | 1996-10-18 | David Fabrice | Accroissement genique, un procede d'amplicication genique isotherme et ses applications |
US5545539A (en) * | 1994-10-18 | 1996-08-13 | Genzyme Corporation | Method for nucleotide sequence amplification |
US6270962B1 (en) * | 1995-01-30 | 2001-08-07 | The Regents Of The University Of California | Methods for the elimination of DNA sequencing artifacts |
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US6114150A (en) * | 1995-11-29 | 2000-09-05 | Yale University | Amplification of nucleic acids |
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US6150094A (en) * | 1996-05-23 | 2000-11-21 | Qiagen Gmbh | Use of an osmolyte for reducing or abolishing no-covalent interactions of biological molecules to inert surfaces |
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US6200757B1 (en) * | 1999-01-19 | 2001-03-13 | Dade Behring Inc. | Method for controlling the extension of an oligonucleotide |
US6951722B2 (en) * | 1999-03-19 | 2005-10-04 | Takara Bio Inc. | Method for amplifying nucleic acid sequence |
ES2447419T3 (es) * | 1999-09-13 | 2014-03-12 | Nugen Technologies, Inc. | Composiciones para la amplificación isotérmica lineal de secuencias de polinucleótidos |
US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
AU2001234608A1 (en) * | 2000-01-28 | 2001-08-07 | Genetrace Systems, Inc. | Methods for analysis of gene expression |
AU3806701A (en) * | 2000-02-07 | 2001-08-14 | Illumina Inc | Nucleic acid detection methods using universal priming |
ATE492652T1 (de) * | 2000-02-07 | 2011-01-15 | Illumina Inc | Nukleinsäuredetektionsverfahren mit universellem priming |
ES2464132T3 (es) * | 2000-04-07 | 2014-05-30 | Eiken Kagaku Kabushiki Kaisha | Procedimiento de amplificación de ácido nucleico utilizando como molde ácido nucleico bicatenario |
CA2460639C (en) * | 2001-09-19 | 2013-07-16 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
US7476519B2 (en) * | 2002-07-19 | 2009-01-13 | Joseph Monforte | Strategies for gene expression analysis |
CA2498764C (en) * | 2002-09-20 | 2015-11-10 | New England Biolabs, Inc. | Helicase dependent amplification of nucleic acids |
WO2004042034A2 (en) * | 2002-11-04 | 2004-05-21 | Fred Hutchinson Cancer Research Center | Method for the linear amplification of complementary dna |
US7374885B2 (en) * | 2004-08-27 | 2008-05-20 | Gen-Probe Incorporated | Single-primer nucleic acid amplification methods |
US20060172324A1 (en) * | 2005-01-28 | 2006-08-03 | Roche Molecular Systems, Inc. | Methods of genotyping using differences in melting temperature |
-
2006
- 2006-09-06 AT AT06803026T patent/ATE550441T1/de active
- 2006-09-06 JP JP2008530153A patent/JP2009506788A/ja not_active Withdrawn
- 2006-09-06 US US11/517,117 patent/US20070054301A1/en not_active Abandoned
- 2006-09-06 EP EP06803026A patent/EP1929045B9/de not_active Not-in-force
- 2006-09-06 WO PCT/US2006/034663 patent/WO2007030505A1/en active Application Filing
- 2006-09-06 CA CA002621146A patent/CA2621146A1/en not_active Abandoned
- 2006-09-06 AU AU2006287548A patent/AU2006287548A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1929045B9 (de) | 2012-06-20 |
WO2007030505A1 (en) | 2007-03-15 |
US20070054301A1 (en) | 2007-03-08 |
EP1929045B1 (de) | 2012-03-21 |
EP1929045A1 (de) | 2008-06-11 |
AU2006287548A1 (en) | 2007-03-15 |
JP2009506788A (ja) | 2009-02-19 |
CA2621146A1 (en) | 2007-03-15 |
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