MX2020014063A - Kits de secuenciacion. - Google Patents
Kits de secuenciacion.Info
- Publication number
- MX2020014063A MX2020014063A MX2020014063A MX2020014063A MX2020014063A MX 2020014063 A MX2020014063 A MX 2020014063A MX 2020014063 A MX2020014063 A MX 2020014063A MX 2020014063 A MX2020014063 A MX 2020014063A MX 2020014063 A MX2020014063 A MX 2020014063A
- Authority
- MX
- Mexico
- Prior art keywords
- precursor composition
- matrix precursor
- encapsulation matrix
- radical
- chambers
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0663—Stretching or orienting elongated molecules or particles
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/12—Specific details about manufacturing devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0819—Microarrays; Biochips
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dispersion Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Un ejemplo de un kit de secuenciación incluye una celda de flujo, una composición de precursor de matriz de encapsulación y un iniciador de radicales. La celda de flujo incluye una pluralidad de cámaras y cebadores unidos dentro de cada una de la pluralidad de cámaras. La composición de precursor de matriz de encapsulación consiste en un fluido, un monómero o polímero que incluye un grupo funcional generador de radicales y alargador de la cadena, una fuente de radicales y un reticulante. El iniciador de radicales es parte de la composición de precursor de matriz de encapsulación o es un componente separado.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962798354P | 2019-01-29 | 2019-01-29 | |
| PCT/US2020/014775 WO2020159795A2 (en) | 2019-01-29 | 2020-01-23 | Sequencing kits |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020014063A true MX2020014063A (es) | 2021-05-27 |
Family
ID=71733374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020014063A MX2020014063A (es) | 2019-01-29 | 2020-01-23 | Kits de secuenciacion. |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US11535890B2 (es) |
| EP (1) | EP3918049A4 (es) |
| JP (1) | JP2022517887A (es) |
| KR (1) | KR20210120822A (es) |
| CN (1) | CN112739810A (es) |
| AU (1) | AU2020215640A1 (es) |
| CA (1) | CA3121456A1 (es) |
| MX (1) | MX2020014063A (es) |
| SG (1) | SG11202012753SA (es) |
| WO (1) | WO2020159795A2 (es) |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6893816B1 (en) | 1993-10-28 | 2005-05-17 | Houston Advanced Research Center | Microfabricated, flowthrough porous apparatus for discrete detection of binding reactions |
| US6465174B1 (en) | 1996-01-26 | 2002-10-15 | University Of Chicago | Rapid method to detect duplex formation in sequencing by hybridization methods, a method for constructing containment structures for reagent interaction |
| EP2295988A2 (en) | 1996-12-31 | 2011-03-16 | High Throughput Genomics, Inc. | Multiplexed molecular analysis apparatus and its fabrication method |
| EP2177627B1 (en) | 1999-02-23 | 2012-05-02 | Caliper Life Sciences, Inc. | Manipulation of microparticles in microfluidic systems |
| US6682702B2 (en) | 2001-08-24 | 2004-01-27 | Agilent Technologies, Inc. | Apparatus and method for simultaneously conducting multiple chemical reactions |
| WO2005043154A2 (en) | 2003-10-27 | 2005-05-12 | Massachusetts Institute Of Technology | High density reaction chambers and methods of use |
| US7713736B2 (en) | 2004-11-24 | 2010-05-11 | Wisconsin Alumni Research Foundation | Cell mimic platform and method |
| EP3722409A1 (en) | 2006-03-31 | 2020-10-14 | Illumina, Inc. | Systems and devices for sequence by synthesis analysis |
| WO2009023257A1 (en) | 2007-08-15 | 2009-02-19 | Massachusetts Institute Of Technology | Microstructures for fluidic ballasting and flow control |
| JP4987088B2 (ja) | 2008-01-08 | 2012-07-25 | 日本電信電話株式会社 | フローセル |
| WO2010003132A1 (en) | 2008-07-02 | 2010-01-07 | Illumina Cambridge Ltd. | Using populations of beads for the fabrication of arrays on surfaces |
| WO2010026950A1 (ja) * | 2008-09-08 | 2010-03-11 | 株式会社日立製作所 | Ｄｎａ検出装置、及びｄｎａ検出デバイス、並びにｄｎａ検出方法 |
| EP2367958B1 (en) | 2008-11-25 | 2017-08-23 | Gen-Probe Incorporated | Compositions and methods for detecting small rnas, and uses thereof |
| US9309557B2 (en) * | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US20120270209A1 (en) | 2009-05-15 | 2012-10-25 | Massachusetts Institute Of Technology | Systems, devices, and methods for specific capture and release of biological sample components |
| US9387476B2 (en) | 2010-10-27 | 2016-07-12 | Illumina, Inc. | Flow cells for biological or chemical analysis |
| EP2718465B1 (en) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Method of making an analyte array |
| EP3305400A3 (en) | 2011-10-28 | 2018-06-06 | Illumina, Inc. | Microarray fabrication system and method |
| US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
| US20160023208A1 (en) | 2013-03-13 | 2016-01-28 | Illumina, Inc. | Multilayer fluidic devices and methods for their fabrication |
| WO2014151961A1 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | Matrix arrays and methods for making same |
| US9409139B2 (en) | 2013-08-05 | 2016-08-09 | Twist Bioscience Corporation | De novo synthesized gene libraries |
| EP3957750A1 (en) * | 2013-12-20 | 2022-02-23 | Illumina, Inc. | Preserving genomic connectivity information in fragmented genomic dna samples |
| CN106459869B (zh) | 2014-03-11 | 2020-01-21 | 伊鲁米那股份有限公司 | 一次性的集成微流体盒及其制备和使用的方法 |
| EP3715468A1 (en) | 2014-06-13 | 2020-09-30 | Illumina Cambridge Limited | Methods and compositions for preparing sequencing libraries |
| WO2016061517A2 (en) | 2014-10-17 | 2016-04-21 | Illumina Cambridge Limited | Contiguity preserving transposition |
| CN107208019B (zh) | 2014-11-11 | 2021-01-01 | 伊鲁米纳剑桥有限公司 | 用于核酸单克隆簇的产生和测序的方法和阵列 |
| CA2970477C (en) | 2014-12-09 | 2022-03-15 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| RU2717491C2 (ru) | 2015-02-10 | 2020-03-23 | Иллюмина, Инк. | Способ и композиция для анализа клеточных компонентов |
| EP3303621A1 (en) | 2015-05-28 | 2018-04-11 | Illumina Cambridge Limited | Surface-based tagmentation |
| EP3329012B1 (en) | 2015-07-27 | 2021-07-21 | Illumina, Inc. | Spatial mapping of nucleic acid sequence information |
| KR102171865B1 (ko) | 2016-05-18 | 2020-10-29 | 일루미나, 인코포레이티드 | 패턴화된 소수성 표면을 사용한 자가 조립된 패터닝 |
| JP6884562B2 (ja) | 2016-11-30 | 2021-06-09 | シスメックス株式会社 | 検体処理方法および検体処理装置 |
| KR102512186B1 (ko) | 2016-12-22 | 2023-03-20 | 일루미나, 인코포레이티드 | 수지 필름 및 패턴화된 중합체층을 포함하는 어레이 |
| WO2018119101A1 (en) | 2016-12-22 | 2018-06-28 | Illumina, Inc. | Flow cell package and method for making the same |
| AU2017386533B2 (en) | 2016-12-29 | 2024-05-09 | Illumina, Inc. | Analysis system for orthogonal access to and tagging of biomolecules in cellular compartments |
| BR112018076259A2 (pt) | 2017-02-21 | 2019-03-26 | Illumina, Inc. | tagmentação usando transposomas imobilizados com ligantes |
| NL2019044B1 (en) * | 2017-05-11 | 2018-11-15 | Illumina Inc | Protective surface coatings for flow cells |
| EP4446437A2 (en) | 2017-08-01 | 2024-10-16 | Illumina, Inc. | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
| KR20210133298A (ko) * | 2017-12-21 | 2021-11-05 | 일루미나, 인코포레이티드 | 하이드로겔 코팅을 가진 플로우 셀 |
| EP3752634B8 (en) | 2018-02-13 | 2023-10-11 | Illumina, Inc. | Dna sequencing using hydrogel beads |
-
2020
- 2020-01-23 MX MX2020014063A patent/MX2020014063A/es unknown
- 2020-01-23 CN CN202080003646.9A patent/CN112739810A/zh active Pending
- 2020-01-23 CA CA3121456A patent/CA3121456A1/en active Pending
- 2020-01-23 AU AU2020215640A patent/AU2020215640A1/en active Pending
- 2020-01-23 EP EP20747860.3A patent/EP3918049A4/en active Pending
- 2020-01-23 SG SG11202012753SA patent/SG11202012753SA/en unknown
- 2020-01-23 KR KR1020207037482A patent/KR20210120822A/ko unknown
- 2020-01-23 JP JP2020572701A patent/JP2022517887A/ja active Pending
- 2020-01-23 US US16/750,897 patent/US11535890B2/en active Active
- 2020-01-23 WO PCT/US2020/014775 patent/WO2020159795A2/en unknown
-
2022
- 2022-10-31 US US17/978,130 patent/US20230063675A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2020159795A3 (en) | 2020-09-17 |
| CN112739810A (zh) | 2021-04-30 |
| WO2020159795A9 (en) | 2020-10-15 |
| EP3918049A4 (en) | 2022-12-07 |
| CA3121456A1 (en) | 2020-08-06 |
| SG11202012753SA (en) | 2021-01-28 |
| KR20210120822A (ko) | 2021-10-07 |
| EP3918049A2 (en) | 2021-12-08 |
| WO2020159795A2 (en) | 2020-08-06 |
| JP2022517887A (ja) | 2022-03-11 |
| US20230063675A1 (en) | 2023-03-02 |
| TW202043486A (zh) | 2020-12-01 |
| AU2020215640A1 (en) | 2021-01-07 |
| US20200239954A1 (en) | 2020-07-30 |
| US11535890B2 (en) | 2022-12-27 |
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