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MX2014003108A - Sonda con especificidad de multiples regiones diana y de caracter tripartito. - Google Patents

Sonda con especificidad de multiples regiones diana y de caracter tripartito.

Info

Publication number
MX2014003108A
MX2014003108A MX2014003108A MX2014003108A MX2014003108A MX 2014003108 A MX2014003108 A MX 2014003108A MX 2014003108 A MX2014003108 A MX 2014003108A MX 2014003108 A MX2014003108 A MX 2014003108A MX 2014003108 A MX2014003108 A MX 2014003108A
Authority
MX
Mexico
Prior art keywords
tripartite
probe
character
target region
multiple target
Prior art date
Application number
MX2014003108A
Other languages
English (en)
Inventor
Ben Cobb
Original Assignee
Epistem Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epistem Ltd filed Critical Epistem Ltd
Publication of MX2014003108A publication Critical patent/MX2014003108A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describen sondas y métodos para detectar polimorfismos, incluyendo repeticiones cortas en tándem, en una secuencia de nucleótido diana. Las sondas incluyen primeras y segundas regiones separadas por una secuencia enlazadora, con las primeras y segundas regiones teniendo temperaturas de fusión discretas con sus respectivas secuencias diana. En una primera modalidad, las primeras y segundas regiones son ambas secuencias reporteras; en una segunda modalidad, una región es una secuencia de anclaje mientras que la otra es una secuencia reportera.
MX2014003108A 2011-09-19 2012-09-19 Sonda con especificidad de multiples regiones diana y de caracter tripartito. MX2014003108A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1116131.2A GB201116131D0 (en) 2011-09-19 2011-09-19 Probe
PCT/GB2012/052305 WO2013041853A1 (en) 2011-09-19 2012-09-19 Probe with multiple target region specificity and of tripartite character

Publications (1)

Publication Number Publication Date
MX2014003108A true MX2014003108A (es) 2014-05-30

Family

ID=44937474

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014003108A MX2014003108A (es) 2011-09-19 2012-09-19 Sonda con especificidad de multiples regiones diana y de caracter tripartito.

Country Status (15)

Country Link
US (1) US20140227683A1 (es)
EP (1) EP2758542B1 (es)
JP (1) JP6097752B2 (es)
KR (1) KR20140063780A (es)
CN (1) CN103998624B (es)
AU (1) AU2012311295A1 (es)
BR (1) BR112014006327A2 (es)
CA (1) CA2849050A1 (es)
ES (1) ES2642275T3 (es)
GB (1) GB201116131D0 (es)
IL (1) IL231443A0 (es)
MX (1) MX2014003108A (es)
RU (1) RU2014115802A (es)
SG (2) SG10201602026SA (es)
WO (1) WO2013041853A1 (es)

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GB201317355D0 (en) * 2013-10-01 2013-11-13 Epistem Ltd Mutation Analysis
WO2015071552A1 (en) * 2013-11-18 2015-05-21 Teknologian Tutkimuskeskus Vtt Multi-unit probes with high specificity and a method of designing the same
US9637791B2 (en) * 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
US11091810B2 (en) * 2015-01-27 2021-08-17 BioSpyder Technologies, Inc. Focal gene expression profiling of stained FFPE tissues with spatial correlation to morphology
US9856521B2 (en) * 2015-01-27 2018-01-02 BioSpyder Technologies, Inc. Ligation assays in liquid phase
US10683534B2 (en) * 2015-01-27 2020-06-16 BioSpyder Technologies, Inc. Ligation assays in liquid phase
CN107109398B (zh) * 2014-12-19 2020-08-18 荣研化学株式会社 单核苷酸多态性检测用寡核苷酸探针及单核苷酸多态性检测方法
JP6685138B2 (ja) * 2016-01-27 2020-04-22 シスメックス株式会社 核酸増幅の精度管理方法、精度管理用試薬およびその試薬キット
WO2019176939A1 (ja) * 2018-03-15 2019-09-19 栄研化学株式会社 一塩基多型検出用オリゴヌクレオチドプローブ、及びシス型－トランス型判別方法

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Also Published As

Publication number Publication date
EP2758542A1 (en) 2014-07-30
WO2013041853A1 (en) 2013-03-28
AU2012311295A1 (en) 2014-04-03
CN103998624B (zh) 2017-03-22
ES2642275T3 (es) 2017-11-16
SG10201602026SA (en) 2016-04-28
JP2014526257A (ja) 2014-10-06
KR20140063780A (ko) 2014-05-27
US20140227683A1 (en) 2014-08-14
IL231443A0 (en) 2014-04-30
JP6097752B2 (ja) 2017-03-15
CN103998624A (zh) 2014-08-20
BR112014006327A2 (pt) 2017-04-11
EP2758542B1 (en) 2017-08-23
CA2849050A1 (en) 2013-03-28
GB201116131D0 (en) 2011-11-02
SG11201400665RA (en) 2014-04-28
RU2014115802A (ru) 2015-10-27

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