Westphal et al., 1968 - Google Patents
Viral DNA in polyoma-and SV40-transformed cell lines.Westphal et al., 1968
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- 1598021416904434028
- Author
- Westphal H
- Dulbecco R
- Publication year
- Publication venue
- Proceedings of the National Academy of Sciences
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Snippet
Materials and Methods.-Viruses: Strains, purification, and DNA extraction of polyoma8'-0 and SV40" 1 have been published elsewhere. The DNA extracted from the viruses was sedimented in a CsCl gradient (1.5 gm per ml 0.02 M Tris-HCl, pH8. 0, 0.002 M EDTA). The …
- 108020005202 Viral DNA 0 title description 37
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
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- C12N15/09—Recombinant DNA-technology
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- C12N15/1034—Isolating an individual clone by screening libraries
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C12Q1/6809—Sequence identification involving differential detection
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- C12P19/28—N-glycosides
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