Papers by Akexander Nosov
Russian Journal of Plant Physiology, 2005
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Russian Journal of Genetics, 2007
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Plant and Cell Physiology, 2003
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Journal of Integrative Plant Biology, 2010
We report the expression profile of acyl‐lipid Δ12‐desaturase (desA) gene from Synechocystis sp. ... more We report the expression profile of acyl‐lipid Δ12‐desaturase (desA) gene from Synechocystis sp. PCC6803 and its effect on cell membrane lipid composition and cold tolerance in prokaryotic (Escherichia coli) and eukaryotic (Solanum tuberosum) cells. For this purpose, a hybrid of desA and reporter gene encoding thermostable lichenase (licBM3) was constructed and used to transform these cells. The expression of this hybrid gene was measured using qualitative (Petri dish test, electrophoregram and zymogram) and quantitative methods (spectrometry and gas liquid chromatography assays). The maximum level of linoleic acid in the bacterial cells containing hybrid gene was 1.9% of total fatty acids. Cold stress tolerance assays using plant damage index and growth parameters showed that cold tolerance was enhanced in primary transgenic lines because of increased unsaturated fatty acid concentration in their lipids. The greatest content of 18:2 and 18:3 fatty acids in primary transgenic plants...
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Acta Agronomica Hungarica, 2011
The responses of plant leaves to chilling were studied in potato (Solanum tuberosum L., cv. Desni... more The responses of plant leaves to chilling were studied in potato (Solanum tuberosum L., cv. Desnitsa) and in its transformants with the native desA gene that encodes the acyl-lipid Δ12-desaturase from the cyanobacterium Synechocystis sp. PCC 6803 and with the hybrid desA gene fused to the reporter gene of thermostable lichenase (licBM3) from Clostridium thermocellum. Cold stress caused a rapid and significant increase in superoxide production and lipid peroxidation (the content of conjugated dienes and malonic dialdehyde) in wild-type plants. By contrast no significant increase was detected in transformed plants under cold stress conditions. This can be attributed to the fact that the overexpression of the acyl-lipid Δ12-desaturase in transformed potato plants promotes fatty acid polyunsaturation and presumably averts the accelerated generation of the superoxide anion, thus suppressing lipid peroxidation under low-temperature stress
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Russian Journal of Plant Physiology, 2007
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Plant Science, 2001
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Russian Journal of Plant Physiology, 2007
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Russian Journal of Genetics, 2007
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Plant Science, 2003
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Pharmaceutical Chemistry Journal, 2004
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Acta Agronomica Hungarica, 2011
... IV GOLDENKOVA-PAVLOVA 2, DA LOS 1, AM NOSOV 1 and TI TRUNOVA 1 ... desA – the gene encoding D... more ... IV GOLDENKOVA-PAVLOVA 2, DA LOS 1, AM NOSOV 1 and TI TRUNOVA 1 ... desA – the gene encoding D12 acyl-lipid desaturase; licBM3 – sequence of the reporter gene en-coding thermostable b-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum; polyA – site for ...
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We propose a new approach to the expression of the gene of interest in plants. The approach is ba... more We propose a new approach to the expression of the gene of interest in plants. The approach is based on the expression hybrid gene in plant. This hybrid gene consists of the sequence of interest, fused with the reporter gene sequence in the reading frame. We constructed hybrid genes desA-licBM3. The DesA gene encodes the acyl-lipid ∆12-desaturase from the cyanobac-
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Molecular Biology, 2003
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Russian Journal of Plant Physiology, 2007
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Russian Journal of Plant Physiology, 2005
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Russian Journal of Genetics, 2007
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Planta Medica, 2002
Growth and development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their ... more Growth and development of Stevia rebaudiana shoots cultivated in the roller bioreactor and their production of steviol glycosides (SGs) were investigated. It was found that, owing to the highly favorable conditions of shoot cultivation created in such an apparatus, the intensity of shoot growth and SG production appeared to be 1.5 - 2.0 times higher than those of the shoots grown in tubes. These results indicate the existence of a positive correlation between these two processes. The data obtained suggest that the enhanced SG production is due to the differentiation of chlorenchyma cells and formation of specific subcellular structures for the glycoside to be accumulated.
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Plant Science, 2000
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Papers by Akexander Nosov