Papers by I. Goldenkova-Pavlova
IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future", 2019
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BackgroundMolecular biology has always shown some similarities with computer science. So, conside... more BackgroundMolecular biology has always shown some similarities with computer science. So, considering transient expression, one can see an analogy with a DDoS attack on a computing system. Like the DDoS attack, transient expression can carry a payload. In particular, analysis of the structure of cell mechanisms and signal amplification in the study of very subtle mechanisms of regulation.ResultsA new vector system for transient expression in plants is described; this system is intended for quantitative analysis of the contribution of regulatory elements to transcription and translation efficiencies. The proposed vector comprises two expression cassettes carrying reporter genes (of the Clostridium thermocellum thermostable lichenase and E. coli
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Applied Microbiology and Biotechnology, 2018
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Applied Microbiology and Biotechnology, 2016
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Biology and Life Sciences Forum, 2020
mRNAs have some regulatory codes which can define the fate of an individual mRNA in translation. ... more mRNAs have some regulatory codes which can define the fate of an individual mRNA in translation. We have developed a flexible online database JetGene (https://jetgene.bioset.org/; accessed on 23 October 2020) that contains cDNA, CDS, 5′-UTR, 3′-UTR sequences from Bacteria, Fungi, Metazoa, Plants, Protists and Vertebrates with the aim of searching regulatory codes in mRNA and studying their correlation with the translational efficiency. It has a friendly interface and puts together a set of tools which are necessary for designing experiments. JetGene allows doing a benchmark analysis of sequences, namely: (1) to estimate the variation of length, nucleotide composition, frequency of codon usage, to analyze GC-content, CpG-islands, to study nucleotides surrounding the start codon and much more; (2) to identify and define the statistically significant representation of potential regulatory contexts at mRNA with different translation efficiency. A user can make a bioinformatics analysis ...
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Book of proceedings of the All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental" (parts I, II), Jul 1, 2018
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Journal Genetika, 2012
A set of module vectors for stable or transient gene expression in plants was constructed with re... more A set of module vectors for stable or transient gene expression in plants was constructed with regard to the majority of factors ensuring efficient heterologous gene expression in plants. The vectors are convenient to clone new regulatory elements and genes of interest via simple molecular cloning procedures. The vectors can be used to obtain transgenic plants with stable heterologous gene expression as well as to achieve transient expression because one vector includes the gene for the tomato bushy stunt virus p19 protein, which acts as a suppressor of posttranscriptional gene silencing.
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Izvestiia Akademii nauk. Seriia biologicheskaia
This paper reports on morphophysiological and biochemical characteristics of control and potato p... more This paper reports on morphophysiological and biochemical characteristics of control and potato plants (Solarium tuberosum L., Skoroplodnyi cultivar) transformed with the Δ12 acyl-lipid desaturase gene (desA) grown long-term in vitro. The transformed plants showed faster growth and faster ontogenesis as compared to controls, which was accompanied with changes in the accumulation of photosynthetic pigments (chlorophylls a and b, carotenoids) and phenolic compounds, including flavonoids in the leaves. These characteristics were pronounced to a high degree in Line II plants with high expression rates of the desA gene, whereas Line I plants (moderate expression rate) were similar to control plants in many parameters.
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Молекулярная биология
Three genes mutations in which cause familial forms of Alzheimer's disease are known to date:... more Three genes mutations in which cause familial forms of Alzheimer's disease are known to date:PSEN1, PSEN2 and APP; and APOE gene polymorphism is a strong risk factor for Alzheimer's disease. We have evaluated allele and genotype frequency distribution of rs11136000 polymorphism in clusterin (CLU) gene (or apolipoprotein J, APOJ) in populations of three Russian regions and i nAlzheimhner's diseasepatients. Genome-wideassociation studies in samples from several European populations have recently revealed highly significant association o fCLU gene with AD (p = 8.5 x 10(-10)). We found no differences in allele and genotype frequencies of rs11136000 between populations from Moscow, Ural and Siberia regions. The allele frequencies are close to those in European populations. The genetic association analysis in cohort of Alzheimer's disease patients and normal individuals (>500 individuals ineach group) revealed no significant association of the rs11136000 polymorphism in...
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Молекулярная биология
Ruacetyltransferase 2 (NAT2) is one of key enzymes of the second phase of biotransformation that ... more Ruacetyltransferase 2 (NAT2) is one of key enzymes of the second phase of biotransformation that metabolize genotoxic compounds such as carcinogens and mutagens in different types of cells. There is a correlation between the decreasing activity of NAT2 gene product and the sensitivity to harmful environmental factors that increase the risk of occurrence of different multifactorial diseases, including dermatological ones like psoriasis. We developed the NAT2-biochip for 17 SNPs. The biochip was been tested on 279 clinical DNA samples from 180 patients with psoriasis and 99 healthy individuals, residents of Moscow. We found only six SNPs that were significant for European populations (282C > T, 341T > C, 481C > T, 590G > A, 803A > G and 857G > A). The analysis in psoriasis group did not show any genotype association. The increase in frequency of a slow acetylation phenotype in group of patients with type II psoriasis and in group of patients with normosthenic constit...
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Journal Genetika, 2006
Psoriasis, a multifactorial disease with genetic predisposition, has been used as an example to s... more Psoriasis, a multifactorial disease with genetic predisposition, has been used as an example to study the role of the ethnic background in multifactorial diseases in the Dagestan population. The individual information card (IIC) is proposed as the main tool for correct collection and processing of information. The results of the study demonstrate that the Dagestan population is a convenient and adequate model population for studying multifactorial diseases, such as psoriasis, and may serve as an object for studying the role of heredity in the etiologies and pathogeneses of this and other multifactorial diseases.
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TSitologiia i genetika
Transgenic popato plants have been created which express recombinant proteins, analogues of spidr... more Transgenic popato plants have been created which express recombinant proteins, analogues of spidroin 1, the protein of the cobweb skeleton thread. Expression of the hybrid spidroin 1 genes possessing some repeated sequences retains both in the model test-tube-growing plants and in the crops. Expression level of the synthetic spidroin 1 genes and the level of accumulation of their products in plants depend on the type of promoter, number of repeats, organ specificity and plant species but not on the duration of plant material storage. The results show that the strategy based on constuction and expression of hybrid proteins which include the reporter protein makes it easier to select and analyse expression of hybrid proteins in transgenic organisms.
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TSitologiia i genetika, 2007
Experimental models of the potato primary transgenic plants which express the hybrid gene cry3aM-... more Experimental models of the potato primary transgenic plants which express the hybrid gene cry3aM-licBM2 have been created. Modecular analysis and the biotests of the experimental models allow proposing a new system of cry genes expression in plants. The system is based on the expression of hybrid genes possessing the sequence of reporter lichenase gene and the use as a regulator element of a light-induced promoter providing preferential expression of the controled genes only in green plant tissues (leaves)--the target tissues for pests. The lichanase presence in hybrid proteins facilitates selection and analysis of the expression level of the hybris proteins in transgenic organisms. Basing on the lichenase properties in hybrid proteins it seems possible to use this reporter system for transgene monitoring in agrocoenosis as this system is rather simple and precise and does not need large material and time expenses.
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IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future", 2019
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IX Congress of society physiologists of plants of Russia "Plant physiology is the basis for creating plants of the future", 2019
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Papers by I. Goldenkova-Pavlova