XN Series Technology
XN Series Technology
XN Series Technology
OBJECTIVES
1
Accuracy
BEYOND TECHNOLOGY
BEYOND EXPECTATIONS
Dependability
Quality
NEXT ADVANCED
GENERATION TOOLS &
DIAGNOSTICS TECHNOLOGIES
greater accuracy, superior insight and
specificity, productivity control, enhancing
speed to treatment
HARMONIZED
PROCESS
SUPPORT
OPTIMIZATION
driving improvement,
automatically
supporting your staff,
balances work-flow
helping you plan for
and routine tasks
the future
NEXT ADVANCED
GENERATION TOOLS &
DIAGNOSTICS TECHNOLOGIES
greater accuracy, superior insight and
specificity, productivity control, enhancing
speed to treatment
2
THE MOST ADVANCED HEMATOLOGY
DIAGNOSTICS ON THE MARKET
Advanced
Body Fluid
Clinical
Differentials
Parameters
Rerun/Reflex Fluorescent
Testing Platelet
XN-SERIES
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N E X T G E N E R AT I O N D I A G N O S T I C S
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SA-01 SAMPLER VERSUS SA-10
SA‐01 SA‐10
Smart Start
Reflex/Rerun capabilities
Rack bar code read
capability
MAP tubes in rack mode
MAP tubes in manual
mode
My Sysmex Compatible
© 2015 Sysmex America, Inc. All rights reserved.
XN-Series Technology
N E X T G E N E R AT I O N D I A G N O S T I C S
RBC,
WBC / RET /
PLT, NRBC DIFF
BASO PLT‐O
Hgb
RBC,
PLT, WNR WDF RET PLT‐F
Hgb
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N E X T G E N E R AT I O N D I A G N O S T I C S
Hemoglobin
Absorbance / Sulfolyser
N E X T G E N E R AT I O N D I A G N O S T I C S
Reportable Parameters
RBC
Platelets
Reagents
CELLPACK DCL
CELLPACK DST*
*Used with RU-20
N E X T G E N E R AT I O N D I A G N O S T I C S
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N E X T G E N E R AT I O N D I A G N O S T I C S
WNR Channel
White Cells, NRBCs
WNR CHANNEL
NRBC RBC
Polymethine Dye
© 2015 Sysmex America, Inc. All rights reserved.
7
WNR CHANNEL
FSC
BASO
NRBC
WBC
Debris
SFL
© 2015 Sysmex America, Inc. All rights reserved.
SFL SFL
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MEGAKARYOCYTES
MEGAKARYOCYTES
WNR CHANNEL
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WDF Channel
WBC Differentials by Fluorescence
WDF CHANNEL
Polymethine Dye
© 2015 Sysmex America, Inc. All rights reserved.
WDF CHANNEL
MONO
IG
LYMPH
NEUT+BASO
Debris
EO
© 2015 Sysmex America, Inc. All rights reserved.
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IG – XN DIFF ABSTRACT
SAFLAS method
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WDF CHANNEL
Enhanced Flagging
Better separation between lymphs and monos (SAFLAS)
Better identification of platelet clumps (multiple channel
detection)
Auto-correction of lymphs when NRBCs are present
6-part differential (including IG)
WB (Whole Blood) mode LW (Low WBC) mode
Analysis Time extended to 3 times the Whole Blood WBC count time
© 2015 Sysmex America, Inc. All rights reserved.
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Body Fluid Analysis
Using RBC/PLT & WDF Channels
Body fluid mode (target species)
Pleural fluid
MN
Peritoneal fluid
MO-BF
NE-BF
EO-BF
WDF Scattergram
© 2015 Sysmex America, Inc. All rights reserved.
No pre-analysis preparation
No additional reagents
Automated background counts
Rapid analysis
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PLT-F Channel
PLaTelets by Fluorescence
PLT-F CHANNEL
Possible interferences
RBC or WBC fragments counted as platelets: falsely high
Microcytic RBCs counted as platelets: falsely high
Large platelets counted as RBC: falsely low
PLT-F CHANNEL
Oxazine Dye
© 2015 Sysmex America, Inc. All rights reserved.
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PLT-F CHANNEL
RBC IPF
PLT-F
Platelet
RBC
Fragment
Transmitted
light
• CD41/CD61 positive = Platelets
• Staining by PLT‐F dedicated reagent
– Platelets ‐ Strong, especially within the cell
– RBC fragments ‐ Weak, only cell membrane
CD61/PLT-F
© 2015 Sysmex America, Inc. All rights reserved.
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DxH 800 Platelet 114
Manual plt estimate 200
XE XN
PLT-I = 477*109/L PLT-I = 514*109/L
PLT-0 = 111*109/L PLT-F = 140.8*109/L
PLT-CD61 =152.2*109/L PLT-CD61 = 152.2*109/L
IPF% = 13.9% IPF%= 12.9%
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IMPROVED PERFORMANCE OF PLT-F
Acute Promyelocytic Leukemia / chemo: white blood cell fragments
XE: PLT- O XN: PLT-F
WBC cytoplasm
WBC cytoplasm
fragments
fragments
IPF
XE PLT-F
XN
PLT-I = 25*109/L
PLT-I = 28*109/L
PLT-0 = 181*109/L
PLT-F = 24.2*109/L
PLT-CD61 = 24.2*109/L
PLT-CD61 = 24.2*109/L
IPF% = 41.2%
IPF% = 1.1%
IPF# = 74.6*109/L
IPF# = 0.3*109/L
© 2015 Sysmex America, Inc. All rights reserved.
The feature:
Huge micro-spherical FRCs appeared because of heat shock.
PLT-F 228
CD61 204
Day 2
PLT-F
PLT
(x10^3/uL)
PLT-I
PLT-I 795
PLT-F 161
CD61 150
© 2015 Sysmex America, Inc. All rights reserved.
17
PLT COUNT OVER TIME IN A SEVERE
BURN INJURY PATIENT
Day 3
PLT-F
PLT
(x10^3/uL)
PLT-F 116
CD61 115
Day 4
PLT-F
PLT
(x10^3/uL)
PLT-I
PLT-I 201
PLT-F 54
CD61 51
© 2015 Sysmex America, Inc. All rights reserved.
PLT-F CHANNEL
RET Channel
RETiculocytes, IRF, RET-He
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RET CHANNEL
Polymethine Dye
© 2015 Sysmex America, Inc. All rights reserved.
RET CHANNEL
RET
IRF
PLT
RETICULOCYTE PARAMETERS
• Reticulocytes
– # and % of immature RBC’s
• Immature Reticulocyte
Fraction
– Newly released from the
marrow, a direct cellular
measurement of
erythropoiesis
• Reticulocyte Hemoglobin
– Direct cellular measure of iron
availability
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RETICULOCYTE CHANNEL
Operational Efficiency
Reduced Interference From:
WBCs
Howell Jolley Bodies
Parasites
Sickle cells
Quick and automatic
Summary
W
N
BASO LYMPH MONO Granulocyte NRBC RBC
R
W
D
F
LYMPH MONO NEUT EO Atypical LYMPH Immature WBC
R P
E L
T WBC RET RBC PLT T RBC PLT IPF
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XN TECHNOLOGY SUMMARY
XN TECHNOLOGY SUMMARY
WNR Channel WDF Channel
• NRBCs on every CBC • 6‐Part Diff
• No WBC Correction • Diffs on low WBC
• IG % & #
Reduce bottlenecks to
Improve TAT while
Adding clinical value
RET Channel PLT‐F Channel
• RET, IRF & RET‐He • Robust 2o Method
• Production View • Reflexive, with IPF
• Functional View • No operator interaction
necessary
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XN Technology
Reagents – RU-20
WHY IT MATTERS……
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WHY IT MATTERS……
Thank You
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