Ketamine 001
Ketamine 001
Ketamine 001
1. SYNONYMS
CFR: Ketamine
2.2. SOLUBILITY
Form A C E H M W
Base I* PS I* *** FS FS
A = acetone, C = chloroform, E = ether, H = hexane, M = methanol and W = water, VS = very soluble, FS =
freely soluble, S = soluble, PS = sparingly soluble, SS = slightly soluble, VSS = very slightly soluble and I =
insoluble.
3. SCREENING TECHNIQUES
Visualization
Relative Rf
System System
COMPOUND TLC 15 TLC 6
Method KETA-GCS1
Method KETA-LCS1
Injection Volume: 5 L
Samples are to be dissolved in buffer: acetonitrile 92:8, sonicated, then filtered with a 0.45-micron filter.
Ketamine is often found as a powder or in an aqueous form. The powder can be extracted with an organic
solvent, such as chloroform and the aqueous form may be evaporated to obtain Ketamine. Ketamine has a
dissociation constant (pKa) of 7.5 and may be extracted from aqueous alkaline solutions using organic solvents.
5. QUANTITATIVE PROCEDURES
Method KETA-GCQ1
Sample Preparation:
Accurately weigh an amount of sample into an appropriate volumetric flask so the final concentration
approximates the standard concentration or falls within the linear range. Dissolve the sample with a small
amount of methanol. Add an appropriate amount of internal standard stock solution diluting it to a ratio of 1 to
10 with chloroform.
Method KETA-LCQ1
Sample Preparation:
Accurately weigh an amount of sample into a volumetric flask and dilute with buffer solution. If necessary
dilute the sample so the final concentration approximates the standard concentration or falls within the linear
range. Filter sample with a 0.45-micron filter.
Injection Volume: 5 L
Method KET-CEQ-1
Sample Preparation:
Accurately weigh an amount of sample into a volumetric flask and add the appropriate amount of internal
standard stock solution. Dilute with 0.01 N HCl so that the concentration approximates that of the standard.
Filter sample with a 0.45-micron filter.
Voltage: 14.5 kV
Temperature: 15C
6. QUALITATIVE DATA
SOLVENT MAXIMUM
ABSORBANCE (NM)
See spectra on the following pages for FT-IR, Raman, Mass Spectrometry, Nuclear Magnetic Resonance, and
Vapor Phase IR.
7. REFERENCES
Clarke, E.G.C., Analysis of Drugs and Poisons, 3rd Edition, The Pharmaceutical Press, 2004.
Budavari, S., The Merck Index, 13th Edition, Merck and Co., Inc., 2001.
Butler, W. P., Methods of Analysis for Alkaloids, Opiates, Marihuana, Barbiturates and Miscellaneous Drugs,
The Internal Revenue Service.
8. ADDITIONAL RESOURCES
Forendex
Wikipedia
FTIR: Ketamine base in KBr
16 scans; 4 cm-1 resolution
100
95
90
85
80
428.76
1129.53
717.67
1428.83
75
2944.07
%T
70
1035.45
65
60
55
3352.15
751.69
50
1700.34
45
90
80
70
60
50
%T
40
1118.01
2447.49
30
572.86
1381.52
2866.71
20
2688.29
773.46
10
1461.03
1449.81
1428.84
1405.63
1721.44
-0
85
80
75
70
65
2685.35
%T
1382.30
1043.77
60
939.17
707.77
55
474.75
1448.68
1117.92
50
718.91
1718.57
45
1460.46
40
572.56
35
772.05
30
90
85
80
75
70
65
%T
60
55
50
45
40
35
30
25
20
3000 2000 1500 1000 500
Wavenumbers (cm-1)
RAMAN: Ketamine HCl
256 scans; 4.0 nm resolution InGaAs Detector, Nicolet 6700
26 K ETA MINE HC L (1.2W)
24
22
20
18
16
14
Int
12
10
11
10
9
Int 6
80
70
60
50
40
182
30
209
20 145
115 152
90
80
(Ketamine + H+)+
70
60
50
40
240.0
30
20
10 207.0 241.0
0
50 100 150 200 250 300 350
m/z
MS/MS: Ketamine in MeOH, electrospray ionization
MS/MS positive ion mode; quadrupole ion-trap analyzer.
Ketamine_MSMS #26-53 RT: 0.32-0.54 AV: 14 NL: 3.92E7
F: + c ESI d Full ms2 237.98@35.00 [ 55.00-250.00]
220.2
100
90
80
MS/MS
70 35 eV
60
50
40
207.0
30
20
221.1
10
179.0
0
60 80 100 120 140 160 180 200 220 240
m/z
IR (VAPOR PHASE) Ketamine
1 mg/mL MeOH HP-5, 25 m, 0.32 mm ID, 1.5 scans/s, 4 cm-1 resolution
100.0
99.5
30 71.80
99.0
11 43.17
10 43.54
14 63.57
98.5
28 76.86
98.0
%T
74 9.34
97.5
97.0
17 27.97
96.5
96.0
29 48.60
95.5
0.95 No. (ppm) Height No. (ppm) Height No. (ppm) Height
1 1.60 0.0166 17 2.01 0.0209 33 4.65 0.7055
0.90
2 1.60 0.0165 18 2.01 0.0174 34 7.45 0.0429
0.85 3 1.62 0.0469 19 2.27 1.0000 35 7.45 0.0533
4 1.63 0.0499 20 2.43 0.0235 36 7.46 0.2269
0.80 5 1.64 0.0419 21 2.44 0.0144 37 7.47 0.1443
6 1.66 0.0338 22 2.45 0.0483 38 7.48 0.2294
0.75
7 1.67 0.0214 23 2.46 0.0548 39 7.49 0.0754
0.70 8 1.73 0.0314 24 2.47 0.0673 40 7.50 0.0144
9 1.74 0.0529 25 2.48 0.0358 41 7.50 0.0238
0.65 10 1.75 0.0457 26 2.50 0.0473 42 7.72 0.0124
Normalized Intensity
0.40
0.35
0.30
0.25
0.20
0.15
0.10
0.05
13 12 11 10 9 8 7 6 5 4 3 2 1 0 -1 -2 -3
Chemical Shift (ppm)
0.09
0.30
0.08
0.07 0.25
Normalized Intensity
0.06
Normalized Intensity
0.20
0.05
0.04 0.15
0.03
0.10
0.02
0.05
0.01
0 0
0.50
0.45
0.40
0.35
0.30
0.25
0.20
0.15
0.10
0.05
-0.05