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    ApoTome2 Quick Guide PDF

    Uploaded by

    Nurizzati Mohd Daud
    1. The document provides instructions for operating a microscope system with an ApoTome attachment, including turning on power supplies and software, placing samples, and capturing images. 2. Steps are outlined for conventional fluorescence imaging and for optical sectioning using the ApoTome, including adjusting the ApoTome slider and capturing z-stacks. 3. Instructions are also provided for viewing and saving captured images, including options for 3D projection of z-stack images.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    ApoTome2 Quick Guide PDF

    Uploaded by

    Nurizzati Mohd Daud
    152 views7 pages
    1. The document provides instructions for operating a microscope system with an ApoTome attachment, including turning on power supplies and software, placing samples, and capturing images. 2. Steps are outlined for conventional fluorescence imaging and for optical sectioning using the ApoTome, including adjusting the ApoTome slider and capturing z-stacks. 3. Instructions are also provided for viewing and saving captured images, including options for 3D projection of z-stack images.

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    Original Title

    ApoTome2 Quick Guide.pdf

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    1. The document provides instructions for operating a microscope system with an ApoTome attachment, including turning on power supplies and software, placing samples, and capturing images. 2. Steps are outlined for conventional fluorescence imaging and for optical sectioning using the ApoTome, including adjusting the ApoTome slider and capturing z-stacks. 3. Instructions are also provided for viewing and saving captured images, including options for 3D projection of z-stack images.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    152 views7 pages

    ApoTome2 Quick Guide PDF

    Uploaded by

    Nurizzati Mohd Daud
    1. The document provides instructions for operating a microscope system with an ApoTome attachment, including turning on power supplies and software, placing samples, and capturing images. 2. Steps are outlined for conventional fluorescence imaging and for optical sectioning using the ApoTome, including adjusting the ApoTome slider and capturing z-stacks. 3. Instructions are also provided for viewing and saving captured images, including options for 3D projection of z-stack images.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 7

    Turn on all power supplies (Sticker No.

    1),
    wait until TFT fully load

    Turn on HBO power box (Sticker No. 2), switch


    on ApoTome.2 control Box (Sticker No. 3) and
    Turn on the PC (Sticker NO.4). Select Zeiss
    user

    Press Load Position on TFT to lower the


    objectives

    Place specimen slide, secure with slide holder

    Press
    on TFT again for objective back to
    working position

    2.

    3.

    4.

    5.

    8.

    Put on a drop of immersion oil on top lens of


    63x and 100x oil objective before use

    Notes:

    In Zen 2011 software, go to Locate tab on left tool


    area to examine the specimen on microscope and
    identify region of interest
    Press Acquisition button to control the microscope
    Under Smart Setup, select respective fluorescent
    used for the sample with the flexibility of changing
    the color according to preference

    2.

    3.
    4.

    Double click on ZEN 2011 software icon on desktop


    to start the software

    1.

    Microscope Operation

    For further details, kindly contact r.ramupillai@zeiss.com

    Turn on Main Switch ( Red labeled A & B)

    1.

    Switching On the System & Working with Specimen

    ApoTome.2 on Axio Imager.Z1 Quick Operating Guide

    For optimum image quality, remember to click Min/


    Max upon Snap

    Leave ApoTome slider OUT for Conventional


    Fluorescence Imaging(1) and gently move in ApoTome
    Slider manually for Optical Sectioning (2)

    Remember to Move the slider out to view image in


    software, reminder messages as below, Click OK

    For further details, kindly contact r.ramupillai@zeiss.com

    8. For Optical Sectioning with ApoTome, move ApoTome slider in manually and set optimum parameter for imaging
    capturing under ApoTome Tab before go to Live to preview and proceed with exposure time adjustment and Snap
    followed by Min/ Max for every single Channel

    7. For Conventional Imaging, Click Snap to capture a 2D Image

    6. Adjust Exposure time for every single channel(dye)

    5. Go to Live to preview the sample (focus on your sample and do necessary adjustment to achieve best image)

    Notes:

    For further details, kindly contact r.ramupillai@zeiss.com

    ApoTome Settings

    For further details, kindly contact r.ramupillai@zeiss.com

    3D Imaging Acquisition (Z-Stack Module)

    Click Gallery to view all the slices capture with Show Dimension Labels for thickness details

    6.

    Click on Start Experiment button to start recording of the Z-Stack.

    5.

    Using focus drive of the microscope to focus on the highest position of the specimen. Click on Set First button to set the highest
    position of the Z-stack. Focus in different direction to the lowest position of specimen. Click on the Set Last button to set the
    lowest position. Click again on Live button to stop specimen scanning (prevent specimen from bleaching).

    3.

    Click on the number of slice thickness after Optimal to optimize number of slices to match the optimal Z-interval to the stack
    size. Under Optimize Sectioning and step section, choose Optimal button to match the optical section from each channel.

    Click on Live button to fast scan the specimen.

    2.

    4.

    Check on checkbox of Z-stack experiment. The Z-Stack Tool bar appears.

    1.

    For further details, kindly contact r.ramupillai@zeiss.com

    1. For 3D Projection, click 3D on the left panel of the Image with various option for 3D view

    3D Projection

    For 3D Images, go to file and select export option

    2.

    For further details, kindly contact r.ramupillai@zeiss.com

    Go to File, Save or Save as, select the folder and Save type

    1.

    To Save Images

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