* Sao ch ADN

AN
↓ sao chep

ADN
Phin ma
↓

ARN (MARN , EARN , 1ARN)

↓ Dich ma

Protein

B 1: nucleotide a trink tu > sa xep

↑
-

3 b can tric-B2 : Can

dang & Gian 3 chien bin
wing- Prang tha rigu hay hoting
& B 3: Qu Ainh Cruc AN
N
ong NS-
>
Mong NST

>
-
1 . Base nito

Nucleotide --
I
> 2
. Acid phosphoric
↳ 3.
Ang deoxyribose (5C)

Nucleoside-Base nito
↓
furnz 5

Phosphodiesten -
> Si hien dien din
- ↑

-
a .
Phosphoric
Clic b
sung >
-

tap new soi kep

Cruc o 2
:

+
Dang B : Chi ye ,
to i
trong m+ 92% H ,
0
,
ric ban
tag phon
~

say phai Ingan long >

-
Van tan
+
Dang A: to fai
trong me 75% H ,
O ,
stric ban .
,
-

i hu hoat trai (hep dainhat-

> kohoting chip (
+
Dan2 :
ng gen,
hic ban
a , , gan sao

>
-

Quy ta ban
tay mus o on cia AN the chic 3- 5

AN sin on goi gon trong than Ab

Y Drong
An
chil
i
Ging chie
KAH
C >
-

Naricio
 the
die Banbo
thing him dia Messon a sth i

saw AN Bao the

* Thinghim ca Meselson ra stahl

-
E
. coli -
c AN
&
T ngan
-

Ngu c Nito
duy nha la amoni

-
co nucleotid th nhom purin va
pyrimidin >
- can cho su
tiny hop ADN

Tach bang
-
a sin ly tam
trong thang nong ot Cesium dorid
The che bo thin N14
+ co : 2
bang
is
Theo che ban bo thin
+ co 1
bing N14 5 ,
: -

-
Gon nu mi stan 30H thank L -
> ke dai d AN

Qu in saw che ADN

pluicing think : d(NMP)n + dNTP >

-
(NMPIn + + PPi

+
Phan ling 1 chin fu rai
sang phai
+ so sanh wr PM :
n ta
↓ TP chat than gia
:

ADN Khun man /AN ku

ring)
Sao ch AN dNTP : A , dT
,
&G , dC

ADN Gain
polymerase >
-

+
Mg2 >
- xic Ain
enzyme
PCR-> Sau che ADN PTN Chief pi
*
trong
=

Meta (t Arong) -
> Vilahuan
Cas kin sao
che Len
-

von >
-
Virus , y t Flgi Air) ca E
. Coli

2
Thang >
-
Th nhan that
Ki Sach A CAN So replicon San phan
Theta
g(Vk) O
ving
-
Von -
1 2 AN
Lon
ring Vang (UK Coli) Co 1 ADN
thing
11
E .

1 AN
ving
Thing thing O
-
whic 2 ADN
thing
 * tham
Enzyme gia saw h AN

Enzyme Helicase : L chu a .

a c trink to bao this H to that man

Ai
+ Gr 3 dre
nans 2
Gan acid nucleic
At

+ C2 Looi -
Rep-protein Ign AN g
tong hop soi so
I Helicase -

the 2
(gan vi so bluon kia -
> I soi muon

SSB (protein gan mach ton /

Enzyme
-

↳ ADN do Helicuse ko chap lo i nhan

>
-
Ko clai

than
guyen thin O
I
I ,I ,echinn
: I

polymerase&/ When
, ,

-
ADN
that :
&, ,V ,
G ,
E

5'-3' pol 3-5" exo 5-3'xo Chic

ADN pol nang
I C C Co
Theythe primer ARN

I C co O Sa chea ADA
Co Co
= Ko di A N

E Co
co
O
Q O
Sa cha AN
Sa da ADN

ADN pol cia nhan that

Polymerase L
B J & E

Avi who
/
4 1 2 ↳ 4
tri Ne Nhen
Ty the Chan
M
i Nhan

dis Kli stai

↑
Scho Saohp Sa chep Sor chep
nanz sao

Step ADN when

ADNtythe ADN han ADN han

ADN pol : L 1
holoenzyme :

Apoenzyme +
Holoenzyme ADN pol
co-factor
Mg2 =

>
- h
ng sa cha
ha
hing tr so AN mai tong hop the
Lung 31-5
> Hoat tin tonuclease 3-5 Li Cai 1 ship to :

> 5 -3'
-

Ca a victim sai
>
-

exo :
-

Enzyme Lipase :

↳ kef ca +
than
AN pol do lin
pyrophosphat NAD
gia

Ai h
Alan Aha sin un cra
Enzyme Topoisomerase : ADN
-

+ topoisomerase I : Ca 1 so
~
ADN ,
& can ATP
+ To poisomerase I : CCF 2 S ADN , CAN ATP

Enzyme Primase
Primoson
+ primate +
polypeptid =

APN helicase kich hoat ran m chin dai

>
-

primate tong hop ARN co 11 + 1 nucleotid

>
-

Lung ca 3-0-> No gn ADN

· Si lin
y
: C1 MAN fai An ing 5
· So kolin : m RN
, m an Okazaki 1 mos

* Qu tri sao ch ADN E Coli.

Khor an : Onic-245 b + pro blo dan

>
-
that an tan ADN

Mo n : AN Helicase SSB protein

,

iso to pomerase

Ring p ma : Primase helicace

,
>
-
m ARN

>
di : ADN pol, ADN ligase

> K Ha : 2 chan ba saw chip gap nhan

protein
-

ket th <Us) ,
Ethic

* Sao Chip ADN rhan that

-

- Sao Ch N - Sao chip ADN th whan that

,

th whan ↳ i stie kahe can

nugen thing I 2020 chep
↳

↳
1 oti khi dan

sao chep
1 >
-
ADN

ADN
thing
+ histone >
- mcleosome
ADN
vong
 Ar dien than that
Nhannguyen thing
M

to to dichuyen so mils 50 mu's

ADN pol

So
replicon 1 Chin

Kthus tran 1000-2000 nu 40-300 nu

Okazaki

P di sao chep cham Thank

virus
-

thing in
diejen

U -

ADN ARN

Soi sta
L

Soictos ~

Danging
L
↓

Dang than&
-

-
Sao chip ADN phage T7
-
He gen SAN thing 39, 937 by
Vi tri kho stan chep cach stan fan ben trai (5) bp
-
sao
cung 5 .
900
A an trink te Lo Cas 160 bp (phan
-

ang pha a trai chea tra o tan)

-

gen ma Luc cho AN Lipase SSB ADN pol Ming , ,

- Sao chep ADN phage M13

-
He gen SSADN ving (atonvong)
- Pro SSB bar phi ADN

- ko the
- tong hip ADN pol
-
Tap 1 ban Sao
 Co mit hap to si do
wing
Vung
i
-

T chi
t
TH ARN primer
↓

ITd
In

* Sao ch AN Phage Lambola

-
Think ti b Jung
- Chi di 200 nucleotid

B gi bi
enzyme
oca ↑
UK pla hun
-
Chon qua trik ly gian
G sen : Mohink theta Origen 0 #DaA(yto Who stan saochep(
↑ I gen # Dac (yto rap helicase)
>S

↳ DnaB(helicase) gan ADN d tach mach

↓
- o

muon : Lao chep lan

vig -
> ban saw len te > ban scoction
Perminase

(han din vitrios)

* Sa Sai N

Saochp ADN in citro >

-
ISS 105
/
& saw chep ADN in viro >
- RSS 109

- che sa
sai
Massach Spine e ho

o 3 - 55 >
# Sa sai Rhino sao chep
Enzyme Enzyme
)n
↳
trink t sai D a sai
↳ Ca tocin sai ↓ ↓ ↓
↳ ou sai Basebin h T sai Bat cap sai