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Chapter 2 Answers

1. Griffith showed that nonvirulent bacteria could be transformed to a virulent state if

they were mixed with heat-killed virulent bacteria. He demonstrated that this transformation
was hereditary by showing that the virulence was not only present in the transformed bacteria,
but it was present in their descendents as well. In other words, these transformed cells were
capable of passing the genetic information for virulence to their progeny.
Without knowing whether or not subsequent generations were also virulent, it was also
possible to conclude that the cells became virulent because they received a factor directly
responsible for virulence, rather than the genetic information coding for the factor. In that
case, however, the virulence factor would have been diluted upon division, and would not
have been present in subsequent generations.

2. Both Avery et al. and Hershey and Chase tried to pinpoint the biochemical source of
the genetic material; the specific ways in which they did this, however, differed in several
respects.
Avery et al. used an indirect, functional approach to identify the genetic material. For
example, their assay was based entirely upon the ability to render rough bacteria virulent.
Also, they demonstrated the essential role of nucleic acids in the process not by showing that
nucleic acids were sufficient for the transforming activity, but by showing that they were
simply necessary. The destruction of nucleic acids in their sample using nucleases was
enough to eliminate the transforming ability.
In contrast, Hershey and Chase took a more direct, physical approach to finding the
genetic material. At that time, scientists already had a relatively clear understanding as to
what molecules were present in many viruses. This allowed Hershey and Chase to ask which
molecules are physically transmitted by the virus to the bacterial cell upon infection. To do
this, they used two different radioactive labels to specifically tag both the protein and nucleic
acid components of the virus, and then asked which of these components ends up in the
bacterial cell following infection and in the progeny phage. Their assay was thus based on the
detection of a specific radioactive isotope in the infected cells (and their descendents), rather
than on any functional property of the cells following infection.

3. The absence of a role for nucleic acids was demonstrated by treating the material
responsible for the disease with agents that destroy nucleic acids, such as nucleases or UV
irradiation. While these treatments should destroy whatever nucleic acids are present in the
material, they were found to have no effect on infectivity. In contrast, methods designed to
eliminate proteins in the material, such as treatment with phenol or proteases, were capable of
reducing the infectivity.

4. Chargaff examined the relative proportions of adenine, cytosine, guanine, and thymine
in DNA, and made the key discoveries that the different nucleotides were not all present at the
same concentrations in cells; that their relative levels differed in different organisms; and that
adenosine and thymine, and cytosine and guanine, are present at similar levels. This work
suggested that the genetic information may be somehow encoded by the precise arrangement
of the different nucleotides within the DNA.

5. The high-quality X-ray diffraction images obtained by Wilkins and Franklin suggested
that DNA was helical and was composed of more than one polynucleotide chain. This
knowledge helped Watson and Crick narrow down the possibilities in their work on modeling
the structure of DNA.

6. Meselson and Stahl wanted to address whether replication occurs in a conservative

manner, in which the parental strands are copied but then remain together after replication, a
semi-conservative manner, in which the duplicated DNA includes one parental strand and one
newly synthesized strand, or a distributive manner, in which the new DNA is present in both
strands of the new DNA.
To distinguish between these three possibilities, Meselson and Stahl developed a
method that allowed them to separately label the parental and daughter strands of DNA during
replication and then to ask where each of the strands were in the replicated DNA. In this way,
they were able to address whether all of the “parental” label remained intact after replication,
or whether the parental label and the daughter labels were present in a single double helix of
DNA.
 In their experiments, they grew bacteria in culture medium that contained heavy
isotopes, such as N15, so that all of the DNA in the cells contained the isotope. They then
shifted the cells to (“light”) medium that didn’t contain the heavy isotope and let the cells
undergo one round of DNA replication. They then isolated the DNA from the cells and
separated it in a very precise way that allowed them to distinguish DNA that contained two
heavy strands, two light strands, or one heavy and one light strand. In so doing, they observed
that the newly replicated DNA contained one heavy and one light strand, which is entirely
consistent with semi-conservative replication. Conservative replication, in contrast, would
have yielded DNA with either two heavy or two light strands and distributive replication
would have included strands with an intermediate density between heavy and light.

7. See Figure 2-7 for a depiction of the polymerase reaction. The sequence of the new
strand of DNA is determined by the sequence of the template DNA. This was demonstrated
by incubating DNA of varying nucleotide composition in the presence of polymerase and
nucleoside triphosphates. The nucleotide composition of the DNA strand produced in this
reaction precisely mirrors that of the DNA originally added to the reaction mixture.

8. The conclusion that DNA must not be the template for protein synthesis was made
based on the observation in eukaryotic cells. In these cells, DNA is exclusively present in the
nucleus, yet protein synthesis occurs in the cytoplasm. It was postulated that some sort of
intermediate molecule must carry the information from the nucleus to the site of protein
synthesis.
RNA was identified as the likely template because of its presence in the cytoplasm,
and because, given the structural similarity of RNA to DNA, it was easy to imagine how the
DNA sequence could be copied as RNA, which could then move to the cytoplasm. Also, the
development of cell-free extracts allowed researchers to isolate the various cellular
components that are required for protein synthesis, and this indicated the importance of RNA
in the process. Definitive evidence for the activity of mRNA in protein synthesis was
provided by observations in T4 infected cells, where it was found that, upon infection, RNA is
produced that has an identical base composition to the T4 DNA, and that this RNA binds to
ribosomes, leading to protein synthesis.

9. One major difference lies in the structure of the sugar moiety of the nucleic acid:
ribonucleic acids include a hydroxyl group at the 2’ carbon of the sugar moiety, whereas the
2’ carbon of deoxyribose has only hydrogen. A second difference is that RNA contains the
base uracil, whereas DNA contains thymine. Finally, DNA is almost exclusively present in
its double-stranded form in vivo, whereas RNA is largely single-stranded.

10. The central dogma states that genetic information flows from DNA to RNA to protein.
In other words, DNA stores the genetic information within the chromosomes in the nucleus.
This information is transcribed onto RNA, which then exits the nucleus, and the RNA is then
translated into protein. Even though the dogma is almost always valid, exceptions have been
found such as the copying of DNA from RNA templates during the life cycle of retroviruses.

11. Before Crick’s proposal that an adaptor molecule might intervene between mRNA and
amino acids, scientists had speculated that RNA may fold into precise three-dimensional
forms that specifically recognize particular amino acids. Crick rejected this suggestion as
unlikely, because RNA bases are mostly hydrophilic, yet the side chains of numerous amino
acids are hydrophobic. In addition, the strong structural similarity between certain amino acid
side chains made it difficult to imagine how different RNA structures could accurately
distinguish between them.
The adaptor was ultimately found to be tRNA.

12a. These three types of RNA differ in multiple respects. For example, both rRNA and
tRNA are quite different from mRNA in terms of size and complexity. tRNA molecules are
quite small (less than 100 nucleotides), and are relatively uniform in sequence. rRNA
molecules are rather large and fall into several discrete sizes, and are also relatively uniform
in sequence. mRNA, in contrast, is extremely diverse, both in terms of size—ranging from
very small messages of less than 100 nucleotides to very large messages of many thousands—
and in terms of complexity, as the collection of mRNA sequences in the cell must be
sufficiently diverse to code for all of the different proteins made in the cell. Also, the GC
content of rRNA is relatively high. Finally, the abundance of the three types differs within
the cell, with rRNA making up some 85 percent of the total RNA, tRNA about 10 percent,
and the rest being mRNA.
b. Whereas rRNA binds directly and stably to the ribosomal proteins, mRNA only
interacts transiently with already assembled ribosomes. Also, while a given rRNA molecule
forms part of a single ribosome, a single mRNA molecule can associate with multiple
ribosomes (forming a polyribosome).
13. The codons must have sufficient complexity to be able to code for all 20 amino acids
used during protein synthesis. Codons of only two nucleotides could only specify (42) 16
different combinations and would therefore not be sufficient to specify all of the amino acids.
While four-nucleotide codons would be possible, there would be a great excess of potential
codons compared to the number of different amino acids. This would both needlessly
increase the size of the genome and also require the cell to wastefully produce a much larger,
but unnecessary, number of different tRNA species.

14. Mutant classes C and F should show very little phenotypic difference from the wild-
type gene, whereas classes A, B, D, and E should show a mutant phenotype. In these classes,
the addition of one, two, four, or five nucleotides would change the reading frame of the
coding sequence, altering the identity of all subsequent amino acids and almost certainly
destroying the function of the encoded protein. In contrast, the addition of three or six
nucleotides (in classes C and F) would not change the reading frame but would simply add
one or two amino acids to the protein sequence. While this may have some effect on the
protein’s activity, it is likely that the protein would still function even with these extra amino
acids.
The fact that insertions of any number of nucleotides that is not a multiple of 3 causes
a mutant phenotype indicates that the codons must consist of three nucleotides.

15. They created synthetic RNA molecules of a known sequence, added them to a cell-free
translation system, and examined the amino acid sequence of the proteins that were produced.
The first such molecule they made was poly-U, or UUUUU…, which gave rise to a
polypeptide composed exclusively of the amino acid phenylalanine.
Another random document with
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 "Thirty-two."

"Twenty-five."

"Good heavens," I said, "I've only got seven."

There was a shout of laughter.

"Then you'd better begin," said somebody. "Read them out."

I coughed doubtfully, and began;

"Napoleon."

There was another shout of laughter.

"I am afraid we can't allow that."

"Why ever not?" I asked in amazement.

"Well, you'd hardly find him in a kitchen, would you?"

I took out a handkerchief and wiped my brow. "I don't want to find him
in a kitchen," I said nervously. "Why should I? As a matter of fact, he's
dead. I don't see what the kitchen's got to do with it. Kitchens begin with a
K."

"But the game is called 'Furnishing a Kitchen.' You have to make a list
of things beginning with N which you would find in a kitchen. You
understood that, didn't you?"

"Y-y-yes," I said. "Oh y-y-y-yes. Of course."

"So Napoleon——"

I pulled myself together with a great effort.

"You don't understand," I said with dignity. "The cook's name was
Napoleon."
 "Cooks aren't called Napoleon," said everybody.

"This one was. Carrie Napoleon. Her mistress was just as surprised at
first as you were, but Carrie assured her that——"

"No, I'm afraid we can't allow it."

"I'm sorry," I said; "I'm wrong about that. Her name was Carrie Smith.
But her young man was a soldier, and she had bought a 'Life of Napoleon'
for a birthday present for him. It stood on the dresser—it did, really—
waiting for her next Sunday out."

"Oh! Oh, well, I suppose that is possible. Go on."

"Gnats," I went on nervously and hastily. "Of course I know that——"

"Gnats are spelt with a G," they shrieked.

"These weren't. They had lost the G when they were quite young, and
consequently couldn't bite at all, and cook said that——"

"No, I'm afraid not."

"I'm sorry," I said resignedly. "I had about forty of them—on the
dresser. If you won't allow any of them, it pulls me down a lot. Er—then we
have Nitro-glycerine."

There was another howl of derision.

"Not at all," I said haughtily. "Cook had chapped hands very badly, and
she went to the chemist's one evening for a little glycerine. The chemist was
out, and his assistant—a very nervous young fellow—gave her nitro-
glycerine by mistake. It stood on the dresser, it did, really."

"Well," said everybody very reluctantly, "I suppose——"

I went on hastily:
 "That's two. Then Nobody. Of course, you might easily find nobody in
the kitchen. In fact you would pretty often, I should say. Three. The next is
Noon. It could be noon in the kitchen as well as anywhere else. Don't be
narrow-minded about that."

"All right. Go on."

"Non-sequitur," I said doubtfully.

"What on earth——"

"It's a little difficult to explain, but the idea is this. At most restaurants
you can get a second help of anything for half-price, and that is technically
called a 'follow.' Now, if they didn't give you a follow, that would be a Non-
sequitur.... You do see that, don't you?"

There was a deadly silence.

"Five," I said cheerfully. "The last is Notting Hill. I must confess," I said
magnanimously, "that I am a bit doubtful whether you would actually find
Notting Hill in a kitchen."

"You don't say so!"

"Yes. My feeling is that you would be more likely to find the kitchen in
Notting Hill. On the other hand, it is just possible that, as Calais was found
engraven on Mary's heart, so, supposing the cook died—— Oh, very well.
Then it remains at five."

Of course you think that, as I only had five, I came out last. But you are
wrong. There is a pleasing rule in this game that, if you have any word in
your list which somebody else has, you cannot count it. And as all the
others had the obvious things—such as a nutmeg-grater or a neck of mutton
or a nomelette—my five won easily. And you will note that, if only I had
been allowed to count my gnats, it would have been forty-five.
 II. GETTING THE NEEDLE

He was a pale, enthusiastic young man of the name of Simms; and he

held forth to us at great length about his latest hobby.

"Now I'll just show you a little experiment," he wound up; "one that I
have never known to fail. First of all, I want you to hide a needle
somewhere, while I am out of the room. You must stick it where it can be
seen—on a chair—or on the floor if you like. Then I shall come back and
find it."

"Oh, Mr Simms!" we all said.

"Now, which one of you has the strongest will?"

We pushed Jack forward. Jack is at any rate a big man.

"Very well. I shall want you to take my hand when I come in, and look
steadily at the needle, concentrate all your thoughts on it. I, on the other
hand, shall make my mind a perfect blank. Then your thoughts will
gradually pass into my brain, and I shall feel myself as it were dragged in
the direction of the needle."

"And I shall feel myself, as it were, dragged after you?" said Jack.

"Yes; you mustn't put any strain on my arm at all. Let me go just where
I like, only will me to go in the right direction. Now then."

He took out his handkerchief, put it hastily back, and said: "First, I shall
want to borrow a handkerchief or something."

Well, we blindfolded him, and led him out of the room. Then Muriel got
a needle, which, after some discussion, was stuck into the back of the
Chesterfield. Simms returned, and took Jack's left hand.

They stood there together, Jack frowning earnestly at the needle, and
Simms swaying uncertainly at the knees. Suddenly his knees went in
altogether, and he made a little zig-zag dash across the room, as though he
were taking cover. Jack lumbered after him, instinctively bending his head
too. They were brought up by the piano, which Simms struck with great
force. We all laughed, and Jack apologised.

"You told me to let you go where you liked, you know," he said.

"Yes, yes," said Simms rather peevishly, "but you should have willed
me not to hit the piano."

As he spoke he tripped over a small stool, and, flinging out an arm to

save himself, swept two photograph frames off an occasional table.

"By Jove!" said Jack, "that's jolly good. I saw you were going to do that,
and I willed that the flower vase should be spared. I'm getting on."

"I think you had better start from the door again," I suggested. "Then
you can get a clear run."

They took up their original positions.

"You must think hard, please," said Simms again. "My mind is a perfect
blank, and yet I can feel nothing coming."

Jack made terrible faces at the needle. Then, without warning, Simms
flopped on to the floor at full length, pulling Jack after him.

"You mustn't mind if I do that," he said, getting up slowly.

"No," said Jack, dusting himself.

"I felt irresistibly compelled to go down," said Simms.

"So did I," said Jack.

"The needle is very often hidden in the floor, you see. You are sure you
are looking at it?"

They were in a corner with their backs to it; and Jack, after trying in
vain to get it over his right shoulder or his left, bent down and focussed it
between his legs. This must have connected the current; for Simms turned
right round and marched up to the needle.

"There!" he said triumphantly, taking off the bandage.

We all clapped, while Jack poured himself out a whisky. Simms turned
to him.

"You have a very strong will indeed," he said, "one of the strongest I
have met. Now, would one of the ladies like to try?"

"Oh, I'm sure I couldn't," said all the ladies.

"I should like to do it again," said Simms modestly. "Perhaps you, sir?"

"All right, I'll try," I said.

When Simms was outside I told them my idea.

"I'll hold the needle in my other hand," I said, "and then I can always
look at it easily, and it will always be in a different place, which ought to
muddle him."

We fetched him in, and he took my left hand....

"No, it's no good," he said at last, "I don't seem to get it. Let me try the
other hand...."

I had no time to warn him. He clasped the other hand firmly; and, from
the shriek that followed, it seemed that he got it. There ensued the "perfect
blank" that he had insisted on all the evening. Then he pulled off the
bandage, and showed a very angry face.

Well, we explained how accidental it was, and begged him to try again.
He refused rather sulkily.

Suddenly Jack said: "I believe I could do it blindfold. Miss Muriel, will
you look at the needle and see if you can will me?"
 Simms bucked up a bit, and seemed keen on the idea. So Jack was
blindfolded, the needle hid, and Muriel took his hand.

"Now, is your mind a perfect blank?" said Simms to Jack.

"It always is," said Jack.

"Very well, then. You ought soon to feel in a dreamy state, as though
you were in another world. Miss Muriel, you must think only of the needle."

Jack held her hand tight, and looked most idiotically peaceful. After
three minutes Simms spoke again.

"Well?" he said eagerly.

"I've got the dreamy, other-world state perfectly," said Jack, and then he
gave at the knees just for the look of the thing.

"This is silly," said Muriel, trying to get her hand away.

Jack staggered violently, and gripped her hand again.

"Please, Miss Muriel," implored Simms. "I feel sure he is just going to
do it."

Jack staggered again, sawed the air with his disengaged hand, and then
turned right round and marched for the door, dragging Muriel behind him.
The door slammed after them....

* * * * * * * *

There is a little trick of sitting on a chair and picking a pin out of it with
the teeth. I started Simms—who was all eagerness to follow the pair, and
find out the mysterious force that was drawing them—upon this trick, for
Jack is one of my best friends. When Jack and Muriel came back from the
billiard-room, and announced that they were engaged, Simms was on his
back on the floor with the chair on the top of him—explaining, for the
fourth time, that if the thing had not overbalanced at the critical moment he
would have secured the object. There is much to be said for this view.
 BACHELOR DAYS

THE BUTTER

"You mustn't think I am afraid of my housekeeper. Not at all. I

frequently meet her on the stairs, and give her some such order as "I think
—if you don't mind—I might have breakfast just a little later—er, yes,
about eight o'clock, yes, thank you." Or I ring the bell and say, "I—I—
want-my-boots." We both recognise that it is mine to command and hers to
obey. But in the matter of the butter I have let things slide, until the position
is rapidly becoming an untenable one. Yet I doubt if a man of imagination
and feeling could have acted otherwise, given the initial error. However,
you shall hear.

There are two sorts of butter, salt and fresh. Now, nobody is so fond of
butter as I am; but butter (as I have often told everybody) isn't butter at all
unless it is salt. The other kind is merely an inferior vaseline—the sort of
thing you put on the axles of locomotives. Imagine, then, my disgust, when
I took my first breakfast in these rooms eleven months ago, to find that the
housekeeper had provided me with a large lump of vaseline!

I hate waste in small things. Take care of the little extravagances, I say,
and the big ones will take care of themselves. My first thought on viewing
this pat of butter was, "It is difficult, but I will eat it." My second, "But I
must tell the housekeeper to get salt butter next time."

An ordinary-minded person would have stopped there. I went one

further. My third thought was this: "Housekeepers are forgetful creatures. If
I tell her now, she will never remember. Obviously I had better wait until
this pound is just finished and she is about to get in some more. Then will
be the time to speak." So I waited; and it was here that I made my mistake.
For it turned out that it was I who was the forgetful creature. And on the
fifteenth day I got up to find another large pound of vaseline on my table.
The next fortnight went by slowly. I kept my eye on every day, waiting for
the moment to come when I could say to the housekeeper, "You will be
getting me in some more butter this morning. Would you get salt, as I don't
much like the other?" Wednesday came, and there was just enough left for
two days. I would speak on the morrow.

But, alas! on the morrow there was another new pound waiting. I had
evidently misjudged the amount.

I forget what happened after that. I fancy I must have been very busy, so
that the question of butter escaped me altogether. Sometimes, too, I would
go away for a few days and the old butter would be thrown away and the
new butter bought, at a time when I had no opportunity of defending
myself. However it was, there came a time when I had been three months in
my rooms, and was still eating fresh butter; contentedly, to all appearances
—in the greatest anguish of soul, as it happened. And at the end of another
month I said: "Now, then, I really must do something about this."

But what could I do? After eating fresh butter for four months without
protest I couldn't possibly tell the housekeeper that I didn't like it, and
would she get salt in future. That would be too absurd. Fancy taking four
months to discover a little thing like that! Nor could I pretend that, though I
used to adore fresh butter, I had now grown tired of it. I hate instability of
character, and I could not lend myself to any such fickleness. I put it to you
that either of these courses would have shown deplorable weakness. No, an
explanation with the housekeeper was by that time impossible; and if
anything was to be done I must do it on my own responsibility. What about
buying a pound of salt butter myself, and feeding on it in secret? True, I
should have to get rid of a certain portion of fresh every day, but...

I don't know if you have ever tried to get rid of a certain portion of fresh
butter every day, when you are living in a flat at the very top of chambers in
London. Drop it out of the window once or twice, and it is an accident.
Three times, and it is a coincidence. Four times, and the policeman on duty
begins to think that there is more in it (if I may say so) than meets the eye....
But what about the fire, you will ask. Ah, yes; but I could foresee a day
when there would be no fire. One has to look ahead.

Besides, as I said, I hate waste. As any cook will tell you, the whole art
of housekeeping can be summed up in three words—Watch the butter.

More months passed and more pats of vaseline. Every day made an
explanation more hopeless. I had thoughts at one time of an anonymous
letter. Something in this style:

"MADAM,—One who is your friend says beware of vaseline. All is

discovered. Fly before it is too late. What is it makes the sea so salt? NaCl.
Sodium Chloride. THE BLACK HAND"

That would give her the impression, at any rate, that there were two
kinds of butter. Confound it all, by what right did she assume without
asking that I had a preference for fresh?

I have now been in my rooms nearly a year. Something must be done

soon. My breakfasts are becoming a farce. Meals which I used to enjoy I
now face as an ordeal. Is there to be no hope for me in the future?

Well, there is a chance? I shall have to wait until July; but with
something definite in view I am content to wait.

In July I hope to go to Switzerland for a month. Two days before

returning home I shall write to my housekeeper. Having announced the day
of my return, and given one or two instructions, I shall refer briefly to the
pleasant holiday which I have been enjoying. I shall remark perhaps on the
grandeur of the mountains and the smiling beauty of the valleys, I may
mention the area in square miles of the country....

And I shall dwell upon the habits of the native.

 "... They live (I shall write) in extraordinary simplicity, chiefly upon the
products of their farms. Their butter is the most delightful I have ever tried.
It is a little salt to the taste, but after four weeks of it I begin to feel that I
shall never be able to do without salt butter again! No doubt, as made in
London it would be different from this, but I really think I must give it a
trial. So when you are ordering the things I mentioned for me, will you ask
for salt butter...."

And if that fails there remains only the one consolation. In three years
my lease is up. I shall take a new flat somewhere, and on the very first day I
shall have a word with the new housekeeper.

"By the way," I shall say, "about the butter...."

THE WASHING

Of course, it is quite possible to marry for love, but I suspect that a good
many bachelors marry so that they may not have to bother about the
washing any more. That, anyhow, will be one of the reasons with me. "I
offer you," I shall say, "my hand and heart—and the washing; and, oh, do
see that six tablecloths and my footer shorts don't get sent every week."

We affect Hampstead for some reason. Every week a number of shirts

and things goes all the way out to Hampstead and back. I once sent a
Panama to Paris to be cleaned, and for quite a year afterwards I used to lead
the conversation round to travel, and then come out with, "Ah, I well
remember when my Panama was in Paris...." So now, when I am asked at a
dance, "Do you know Hampstead at all?" I reply, "Well, I only know it
slightly myself; but my collars spend about half the year there. They are in
with all the best people."

I can believe that I am not popular in Hampstead, for I give my

laundress a lot of trouble. Take a little thing like handkerchiefs. My rooms,
as I have mentioned, are at the very top of the building, and there is no lift.
Usually I wait till I am just out into the street before I discover that I have
forgotten my handkerchief. It is quite impossible to climb all the stairs
again, so I go and buy one for the day. This happens about three times a
week. The result is that nearly all my handkerchiefs are single ones—there
are no litters of twelve, no twins even, or triplets. Now when you have a lot
of strangers in a drawer like this, with no family ties (or anything) to keep
them together, what wonder if they gradually drift away from each other?

My laundress does her best for them. She works a sort of birthmark in
red cotton in the corner of each, so that she shall know them again. When I
saw it first I was frightened. It looked like the password of some secret
society.

"Are there many aliens in Hampstead?" I asked the housekeeper.

"I don't know, sir."

"Well, look here, what I found on my handkerchief. That's a secret

signal of some sort, you know, that's what it is. I shall get mixed up in some
kind of anarchist row before I know where I am. Will you arrange about
getting my clothes washed somewhere else, please?"

"That's because you haven't got your name on it. She must mark them
somehow."

"Then, why doesn't she mark them with my name? So much simpler."

"It isn't her business to mark your clothes," said the housekeeper.

That, I suppose, is true; but it seems to me that she is giving us both a

lot of unnecessary trouble. Every week I pick out this decorative design
with a penknife, and every week she works it in again. When you consider
the time and the red cotton wasted it becomes clear that a sixpenny bottle of
marking ink and a quill pen would be cheaper to her in the long run.

But she has a weakness for red cotton. The holes in the handkerchiefs
she works round with it—I never quite understand why. To call my
attention to them, perhaps, and to prevent me from falling through. Or else
to say, "You did this. I only washed up to the red, so it can't be my fault."

If I were married and had a house of my own, there would be no man

below; consequently he wouldn't wear the absurd collars he does. I get
about two of them a week (so even red cotton is not infallible); and if they
were the right size and a decent shape I shouldn't grumble so much. But I
do object to my collars mixing in town with these extraordinary things of
his. At Hampstead, it may be, they have to meet on terms of equality, more
or less; force of circumstances throws them together a good deal. But in
town no collar of mine could be expected to keep up the acquaintance. "You
knew me in the bath," I can imagine one of his monstrosities saying; and,
"When I am in the bath I shall know you again," would be the dignified
reply of my "16-Golf."

Collars trouble me a good deal one way or another. Whenever I buy a

new dozen, all the others seem suddenly to have become old-fashioned in
shape, and of the wrong size. Nothing will induce me to wear one of them
again. They get put away in boxes. Covered with dust, they lie forgotten.

Forgotten, did I say? No. The housekeeper finds them and sends them to
the wash. About a month later she finds them again. She is always finding
clothes which have been discarded for ever, and sending them to the wash.

The mistake is, that we have not yet come to an agreement as to what
really is to go to the wash, and what isn't. There is a tacit understanding that
everything on the floor on Monday morning is intended for Hampstead. The
floor is the linen-basket. It seemed a good idea at the time, but it has its
faults. Things gets on to the floor somehow, which were never meant for the
north-west—blankets, and parts of a tweed suit, and sofa cushions. Things
have a mysterious way of dropping. Half-a-dozen pairs of white flannel
trousers dropped from a shelf one December. A pair of footer shorts used to
go every week—a pair which I would carefully put down to take the bath
water when I had finished with it. I wonder what those shorts thought they
were doing. Probably they quite fancied themselves at football, and boasted
about the goals they shot to companions whom they met at Hampstead.

"You're always here!" a pair of local wanderers would say.

 "My dear man, I play so hard, I don't care how dirty I get."

The irony of it!

But, worst of all, the laundry-book. Every week the housekeeper says to
me, "Would you pay your book now, as it's been owing for a month?" And
every week I pay. That sounds absurd, but I swear it's true. Or else the
weeks go very quickly.

And such amounts! Great ninepences for a counterpane or a tablecloth

or a white tie. Immense numbers of handkerchiefs, counting (apparently)
twelve as thirteen. Quaint hieroglyphics, which don't mean anything but
seem to get added into the price. And always that little postscript, "As this
has been owing for a month, we must request...."

TAKING STOCK

Beatrice has been spring-cleaning me to-day, or rather my clothes. I

said, wasn't it rather early for it, as none of the birds were singing properly
yet, and she had much better wait till next year; but no, she would do it now.
Beatrice is my sister-in-law, and she said—— Well, I forget what she did
say, but she took a whole bundle of things away with her in a cab; and I
know John will be wearing that purple shirt of mine to-morrow. As a matter
of fact, it was a perfectly new one, and I was only waiting till Lent was
over.

Beatrice said the things were all lying about anyhow, and how I ever
found anything to put on she didn't know; but I could have told her that they
were all arranged on a symmetrical plan of my own. Beatrice doesn't
understand the symmetry of a bachelor's mind. I like a collar in each
drawer, and then, whatever drawer you open, there's a collar ready for you.
Beatrice puts them all in one drawer, and then if you're in a hurry, and open
the wrong drawer by mistake, you probably go up to the office in two
waistcoats and no collar at all. That would be very awkward.

Beatrice actually wanted a braces drawer (if she hadn't married John I
should never let her talk to me about braces), but I explained that I had only
one pair, and was wearing those, so that it would be absurd. I expect she
wanted me to think that John had two pairs. All I can say, is, that, if he has,
he ought to be above taking my best shirt....

I don't think the waistcoat drawer will be a success. There are twenty-
three of them, and some of them don't blend at all well. Twenty-three in one
drawer—you know there are bound to be disputes. I see William has got to
the top already. Ah! he was a fine fellow, the first I ever had. I don't quite
know how to describe him, but in colour he was emerald green, with bits of
red silk peeping through. Sort of open-work, you know, only where you
would expect to see me there was more of William. I wore him at Beatrice's
wedding. He would come. Only he wouldn't let me into the vestry. I wanted
to sign my name; all the others were. I have never worn him since that day,
but Beatrice has fished him out, and now he lies on the very top of the
drawer.

Of course it's awfully good of Beatrice to take so much trouble about

my clothes, and I'm extremely grateful, and after all she did marry my
brother John; but I think sometimes she—— Well, here's a case. You know,
when you have twenty-three waistcoats you perhaps run a bit short of—of
other things. So, naturally, the few you have got left, you—— Well,
Beatrice took them all away, and said that as I couldn't possibly wear them
again she'd cut them up for house-cloths. And really—half-way between
winter and summer is a very awkward time for restocking. But I suppose it
is going to be warmer now?

House-cloths! I bet John has a go at them first.

Beatrice found what they call in the profession a "morning-coat and

vest" under the bed, and said that she would take it away and sell it for me.
I like the way she "finds" things which I have been keeping for years under
the bed. It is absurd to talk about "finding" anything in a small flat, because,
of course, it's there all the time: but Beatrice thought that I ought to be
grateful to her for the discovery, so I pretended I was. She said she would
get at least half-a-crown for it; but I said I would rather have the coat.
However, it turned out that I wasn't even to have had the half-crown....

I used to have thirty pairs of old white gloves in a drawer. I would take
them out sometimes, and stroke them affectionately, and say, "Ah, yes,
those were the ones I wore at that absolutely ripping dance when I first met
Cynthia, and we had supper together. You can see where I spilt the ice
pudding." Or—"This was that Hunt Ball, when I knew nobody and danced
with Hildegarde all the time. She wore black; just look at them now." Well,
Beatrice had that drawer out pretty quick. And now they are on their way to
Perth or Paris, or wherever it is; except Hildegarde's pair, which will just do
for the girl when she cleans the grates. I expect she really will get them you
know; because John doesn't dance.

You know, you mustn't make too much fun of Beatrice; she has ripping
ideas sometimes. She filled a "summer-trunk" for me—a trunk full of all the
clothes I am going to want in the summer. She started with a tennis-racquet
(which, strictly speaking, isn't clothes at all), and went on with some of the
jolliest light waistcoats you ever saw; it made me quite hot to look at them.
Well, now, that's really a good idea so far as it goes. But what will happen
when the summer does come? Why, we shall have to go through the whole
business all over again. And who'll arrange the winter-trunk? Beatrice. And
who'll get the pink pyjamas and the green socks that there's really no room
for, dear? Why, John.

Yet I am sorry for John. He was once as I am. What a life is his now.
Beatrice is a dear, and I will allow no one to say a word against her, but she
doesn't understand that trousers must be folded, not hung; that a collar
which has once been a collar can never be opened out and turned into a cuff
(supposing one wore cuffs); and that a school eleven blazer, even if it
happens to be pink, must not be cut down into a dressing-jacket for the little
one. Poor John! Yes, I am glad now that he has that shirt of mine. It is
perhaps a little too bright for his complexion, perhaps he has not quite the
air to carry it off; but I am glad that it is his. Now I think of it I have a tie
and a pair of socks that would go well with it; and even William—can I part
with William?—yes, he shall have William. Oh, I see that I must be kind to
John.

Dear Beatrice! I wonder when I shall have everything straight again.

MEDES AND PERSIANS

I have already said that I am not afraid of my housekeeper, so there is

no need for me to say it again. There are other motives than fear which
prevent a man from arguing with housekeepers; dislike of conversation with
his intellectual inferiors may be one, the sporting instinct is certainly
another. If one is to play "Medes and Persians" properly, one must be a
sportsman about it. Of course, I could say right out to her "Do this," and she
might do it. Or she could say right out to me, "Do that," and I would reply,
"Don't be absurd." But that wouldn't be the game.

As I play it, a "Mede" is a law which she lays down, and to which (after
many a struggle) in the end I submit; a "Persian" is a law which I lay down,
and to which ... after many a struggle ... in the end ... (when it is too late)....
Well, there are many Medes, but so far I have only scored one Persian of
note.

The first Mede was established last winter. For many weeks I had
opened my bedroom door of a morning to find a small jug of cold water on
the mat outside. The thing puzzled me. What do I want with a small jug of
cold water, I asked myself, when I have quite enough in the bath as it is?
Various happy thoughts occurred to me—as that it was lucky, that it
collected the germs, or (who knows?) indicated a wife with five thousand a
year—but it was a month before the real solution flashed across my mind.
"Perhaps," I said, "it was hot once. But," I added, "it must have been a long
time ago."
 The discovery upset me a good deal. In the first place, it is annoying
suddenly to have all one's hopes of a rich wife and protection from disease
dashed to the ground; in the second, I object to anybody but a relation
interfering with my moral character. Here was a comparative stranger trying
to instil the habit of early rising into me by leaving shaving-water outside
the door at three A.M. Was this a thing to be taken lying down?

Decidedly. So I stayed in bed and ignored the water-jug; save that each
morning, as I left my rooms, I gave it a parting sneer. It was gone by the
evening, but turned up again all right next day. After a month I began to get
angry. My housekeeper was defying me; very well, we would see who
could last the longer.

But after two months it was a Mede. Yet I have this triumph over her.
That though I take the water in I ... pour it into the bath and slip back into
bed again. I don't think she knows that.

Since then there have been many Medes. Little ones as to the position of
the chairs; bigger ones as to the number of blankets on the bed. You mustn't
think, though, that I always submit so easily. Sometimes I am firm. In the
matter of "Africa Joe" I have been very firm. Here, I know, I have right on
my side.

A year ago I was presented with a model of an Irish jolting car (with
horse and driver complete), which had been cut out of some sort of black
wood. The thing used to stand over my fireplace. Later on I acquired, at
different times, a grey hippopotamus (in china) and a black elephant. These
I harnessed on in front of the horse; and the whole affair made a very pretty
scene, which was known to my friends as "Sunday in the Forest: Africa Joe
drives his Family to Church." Besides all these I had yet another animal—a
green frog climbing a cardboard ladder. I leant this against the clock. One
had the illusion that the frog was climbing up in order to look at the works
—which was particularly pleasing because the clock didn't go.

Very well. You have the two scenes on the same mantelboard. One, the
frog as Bond Street watchmaker and jeweller, and the other (such is
Empire), Africa Joe in the heart of the forest. And what does the
housekeeper do? If you will believe me, she takes the frog down from the
clock and props him up behind the car, just as though he were getting on to
it in order to go to church with the others!

Now I do put it to you that this is simply spoiling the picture altogether.
Here we have a pleasant domestic episode, such as must occur frequently in
the African forests. Black Joe harnesses his horse, elephant, hippopotamus,
or what not, and drives his family to the eleven o'clock service. And into
this scene of rural simplicity a mere housekeeper elbows her way with
irrevelant frogs and ladders!

It is a mystery to me that she cannot see how absurd her contribution is.
To begin with, the family is in black (save the hippopotamus, who is in a
quiet grey), so is it likely that they would tolerate the presence of a garish
green-and-yellow stranger? (More than likely Joe is a churchwarden, and
has not only himself to think of.) Then, again, consider the title of the
scene: "Africa Joe drives his Family"—not "Africa Joe about to drive." The
horse is trotting, the elephant has one leg uplifted, and even the
hippopotamus is not in a position of rest. How then could the frog put a
ladder up against a moving cart, and climb in? No; here anyhow was a
Mede that must be resisted at all costs. On the question of Africa Joe I
would not be dictated to.

But, after re-emphasising my position daily for three weeks, I saw that
there was only one thing to do. The frog must be sacrificed to the idea of
Empire. So I burnt him.

But it is time I mentioned my one Persian. It was this way. In the winter
I used always to dry myself after the bath in front of my sitting-room fire.
Now, I know all about refraction, and the difficulty of seeing into a room
from outside, and so forth, but this particular room is unusually light,
having six large windows along one of its sides. I thought it proper,
therefore, to draw down the three end blinds by the fireplace; more
especially as the building directly opposite belonged to the Public House
Reform Association. In the fierce light that beats from reform associations
one cannot be too careful.

Little things like blinds easily escape the memory, and it was obvious
that it would be much pleasanter if the housekeeper could be trained always
to leave the end three down. The training followed its usual course.

Every morning I found the blinds up and every morning I drew them
down and left them there. After a month it seemed impossible that I could
ever establish my Persian. But then she forgot somehow; and one day I
woke up to find the three blinds down.

By a real stroke of genius I drew them up as soon as my dressing was

over. Next morning they were down again. I bathed, dried, dressed and
threw them up. She thought it was a Mede, and pulled them down.

But it was a Persian, and, as I pulled them up, I knew that I had scored.

Yet, after all, I am not so sure. For it is now the summer, and I have no
fire, and I do not want the blinds left down. And when I pull them up every
morning I really want to find them up next morning. But I find them down.
So perhaps it really is a Mede. To tell the truth, the distinction between the
two is not so clear as it ought to be. I must try to come to some arrangement
with the housekeeper about it.

THE CUPBOARD

It was the landlord who first called my attention to the cupboard; I

should never have noticed it myself.

"A very useful cupboard you see there," he said, "I should include that
in the fixtures."

"Indeed," said I, not at all surprised; for the idea of his taking away the
cupboard had not occurred to me.

"You won't find many rooms in London with a cupboard like that."