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    Acetone Butanolfermentation 160109053032

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    SHIVANI ITOKAR

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    Acetone Butanolfermentation 160109053032

    Uploaded by

    SHIVANI ITOKAR
    7 views13 pages

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    acetone-butanolfermentation-160109053032

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    7 views13 pages

    Acetone Butanolfermentation 160109053032

    Uploaded by

    SHIVANI ITOKAR

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 13

    Acetone-Butanol

    fermentation
    Noufal N
    S3 MSc Microbiology
    School of Biosciences
    M.G.University
    • 1862 Pasteur discovered butanol fermentation in
    bacteria.
    • 1911 Fernbach described several species of bacteria
    which conduct acetone-butanol-fermentation.

    • 1916 acetone-butanol fermentation


    was developed by Chaim Weizmann
    using Clostridium acetobutylicum.

    • Acetone: production of explosives &


    • Butanol: making synthetic rubbers.
    • In acetone-butanol fermentation, acetone and butanol are
    produced from glucose using strains of Clostridia, which
    are strictly anaerobic bacteria. Further, ethanol is also
    produced.
    • The ABE fermentation produces solvents in a ratio of 3
    parts acetone, 6 parts butanol to 1 part ethanol.
    • Clostridium acetobutylicum is the most well-studied
    and widely used species, although Clostridium
    beijerinckii has also been used with good results.
    • Used for commercial fermentation in Europe and America until
    the 1970s, currently only in China.
    • An obligately anaerobic, Gram-positive and endospore- forming
    rod.
    • Not pathogenic or toxigenic to humans, animals, or plants.

    Clostridium acetobutylicum Clostridium beijerinckii


    Metabolic pathways of A.B.E. fermentation comprise two
    • ƒ
    characteristic phases: Acidogenesis and Solventogenenis .
    Organism:
    1. Clostridium acetobutylicum – 1st organism –
    industrial production of acetone from starch.
    2. Clostridium saccharoacetobutylicum – convert
    molasses into acetone & butanol.

    • submerged cultures
    • Substrate: sterile diluted molasses or cooked corn
    meal.
    • pH : 7.2
    • Type of fermentation: anaerobic
    • By-products : CO₂( preparation of dry ice) & H (fuel)
    • Product recovery : fractional distillation
    • Large bioreactors – 200000-1000000 Lr
    • CO2 was bubbled through the culture to ensure that O2
    was excluded.
    • Fermentation – biphasic Acidogenesis

    Solventogenenis
    • Acidogenesis – forming acetate , butyrate , hydrogen ,
    and CO2.
    • Solventogenesis – forming butanol, acetone , and
    ethanol.
    • After 40-60 hrs – 12-20gm/L of solvent(6B:3A:E1).
    • The solvents was removed by distillation.
    • The remaining microbial dried solids were used as
    high nutrient animal feed.
    `
    uses:
    • Butanol is a valuable solvent for the production of
    lacquer(used to prepare the finish of
    automobiles),latex,enamels….
    • Used as biofuel(it has similar physical properties to
    gasoline).
    • Used as an additive to plastics to keep them flexible.
    • Used as a solvent in the manufacture of antibiotics ,
    vitamins , and hormones.
    • Acetone is important in making cordite.
    Thank
    you

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