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The Big Squeeze Lab

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The​ ​Big​ ​Squeeze​​ ​Lab Name:​ ​ ​___________________________________

Cell​ ​Energy​ ​-​ ​Anaerobic​ ​Respiration ​ ​ ​I​ ​worked​ ​with:​ ​___________________________________

Introduction​:​ ​ ​As​ ​you​ ​know,​ ​there​ ​are​ ​two​ ​types​ ​of​ ​cellular​ ​respiration.
Aerobic​ ​respiration​ ​is​ ​the​ ​breakdown​ ​of​ ​sugars​ ​in​ ​the​ ​presence​ ​of​ ​oxygen​ ​to
produce​ ​ATP​ ​energy,​ ​CO​2​​ ​and​ ​H2​​ O.​ ​ ​Anaerobic​ ​respiration​ ​is​ ​the​ ​breakdown​ ​of
sugars​ ​in​ ​the​ ​absence​ ​of​ ​oxygen​ ​to​ ​produce​ ​ATP​ ​energy.​ ​ ​There​ ​are​ ​two​ ​kinds​ ​of
anaerobic​ ​respiration:​ ​ ​ ​alcoholic​ ​fermentation​ ​and​​ ​lactic​ ​acid​ ​fermentation​.​ ​ ​ ​In
this​ ​lab​ ​we​ ​will​ ​be​ ​examining​ ​why​ ​we​ ​fatigue​ ​and​ ​get​ ​sore​ ​during​ ​prolonged
exercise.​ ​ ​Partners​ ​(2)​ ​will​ ​have​ ​one​ ​tennis​ ​ball​ ​and​ ​take​ ​turns​ ​either​ ​squeezing​ ​or
timing/recording.

Objectives​:​ ​ ​By​ ​the​ ​end​ ​of​ ​this​ ​activity,​ ​you​ ​will​ ​be​ ​able​ ​to…
•​ ​ ​Record​ ​and​ ​graph​ ​experimental​ ​data​ ​and​ ​manipulate​ ​an​ ​experiment​ ​with​ ​testable​ ​hypotheses.
•​ ​ ​Determine​ ​when​ ​muscle​ ​cells​ ​convert​ ​from​ ​the​ ​aerobic​ ​respiration​ ​pathway​ ​to​ ​anaerobic​ ​respiration.
•​ ​ ​Analyze​ ​the​ ​importance​ ​of​ ​the​ ​metabolic​ ​pathway​ ​of​ ​anaerobic​ ​respiration​ ​and​ ​its​ ​effect​ ​in​ ​muscle​ ​cells.

Instructions​:​ ​ ​PART​ ​ONE


1. ​ ​While​ ​1​ ​person​ ​squeezes​ ​(the​ ​“squeezer”)​ ​will​ ​count​ ​how​ ​many​ ​times​ ​they​ ​can​ ​fully​ ​squeeze​ ​a​ ​tennis​ ​ball​ ​over​ ​a
20​ ​second​ ​time​ ​period​.
2. The​ ​“squeezer”​ ​will​ ​rest​ ​for​ ​20​ ​seconds​ ​between​ ​each​ ​of​ ​the​ ​ten​ ​trials​ ​and​ ​write​ ​down​ ​their​ ​count.
3. The​ ​“timer”​ ​will​ ​start​ ​the​ ​stop​ ​watch​ ​and​ ​time​ ​each​ ​20​ ​second​ ​trial​ ​and​ ​each​ ​20​ ​second​ ​rest​ ​period​.
4. After​ ​each​ ​trial​ ​the​ ​“timer”​ ​will​ ​jot​ ​down​ ​how​ ​many​ ​squeezes​ ​their​ ​partner​ ​did​ ​in​ ​the​ ​elapsed​ ​time​ ​on​ ​their
partner’s​ ​Data​ ​table​ ​One.

Data​ ​Table​ ​One:


Trial​ ​1 Trial​ ​2 Trial​ ​3 Trial​ ​4 Trial​ ​5 Trial​ ​6 Trial​ ​7 Trial​ ​8 Trial​ ​9 Trial​ ​10
#​ ​of​ ​squeezes​ ​in
20​ ​seconds

Graph​ ​#​ ​1​:​ ​ ​Be​ ​sure​ ​to​ ​include​ ​a​ ​title​ ​(remember​ ​you​ ​are​ ​comparing​​ ​trials!)​ ​ ​Write​ ​in​ ​an​ ​appropriate​ ​scale​ ​for​ ​the​ ​y-axis.

Title: ___________________________________________________
Instructions​:​ ​ ​PART​ ​TWO
5. ​ ​Let’s​ ​change​ ​things​ ​up​ ​a​ ​bit.​ ​ ​Decide​ ​with​ ​your​ ​partner​ ​on​ ​a​ ​new​ ​amount​ ​of​ ​time​ ​to​ ​“squeeze,”​ ​“rest,”​ ​or​ ​some
other​ ​variable​ ​(switching​ ​to​ ​the​ ​less​ ​dominant​ ​hand,​ ​a​ ​partner​ ​of​ ​a​ ​different​ ​gender,​ ​etc.)

Describe​ ​the​ ​variable​ ​you​ ​will​ ​manipulate​ ​here: ________________________________________________________

Hypothesis​​ ​:​ ​ ​How​ ​do​ ​you​ ​think​ ​that​ ​your​ ​new​ ​“squeeze”​ ​and​ ​“rest”​ ​times​ ​(or​ ​other​ ​variable)​ ​will​ ​affect​ ​your​ ​results?

Data​ ​Table​ ​Two:​​ ​ ​Put​ ​your​ ​ideas​ ​to​ ​the​ ​test!​ ​ ​Make​ ​sure​ ​to​ ​fill​ ​out​ ​your​ ​data​ ​table​ ​(for​ ​each​ ​person)​ ​below.
Trial​ ​1 Trial​ ​2 Trial​ ​3 Trial​ ​4 Trial​ ​5 Trial​ ​6 Trial​ ​7 Trial​ ​8 Trial​ ​9 Trial​ ​10
#​ ​of​ ​squeezes​ ​in
______​ ​seconds

Now,​ ​graph​ ​your​ ​results​ ​again!

___________________________________

Analysis​ ​and​ ​Conclusion​ ​Questions:​​ ​ ​Please​ ​answer​ ​the​ ​following​ ​questions.

1. What​ ​do​ ​“aerobic”​ ​and​ ​“anaerobic”​ ​mean?

2. What​ ​produces​ ​more​ ​ATP​ ​energy?​ ​ ​Aerobic​ ​or​ ​Anaerobic​ ​Respiration?


3. What​ ​type​ ​of​ ​cellular​ ​respiration​ ​were​ ​your​ ​hand​ ​muscles​ ​using​ ​before​ ​squeezing​ ​the​ ​tennis​ ​ball​ ​and​ ​what​ ​type
of​ ​cellular​ ​respiration​ ​were​ ​your​ ​hand​ ​muscles​ ​using​ ​once​ ​you​ ​started​ ​to​ ​get​ ​sore​?)

Before:​ ​ ​___________________________________ After:​ ​ ​________________________________________

4. Did​ ​squeezing​ ​the​ ​tennis​ ​ball​ ​get​ ​easier​ ​or​ ​harder​ ​to​ ​do​ ​over​ ​the​ ​ten​ ​trials?​ ​ ​Explain.

5. ​ ​At​ ​what​ ​point​ ​in​ ​experiment​ ​one​ ​do​ ​you​ ​think​ ​that​ ​your​ ​muscle​ ​cells​ ​converted​ ​from​ ​aerobic​ ​respiration​ ​to​ ​lactic
acid​ ​fermentation?​ ​ ​(Refer​ ​to​ ​your​ ​graph​ ​number​ ​one.)

6. At​ ​what​ ​point​ ​in​ ​experiment​ ​two​ ​do​ ​you​ ​think​ ​that​ ​your​ ​muscle​ ​cells​ ​converted​ ​from​ ​aerobic​ ​respiration​ ​to​ ​lactic
acid​ ​fermentation?​ ​ ​(Refer​ ​to​ ​your​ ​graph​ ​number​ ​two.)

7. Why​ ​did​ ​your​ ​muscles​ ​start​ ​to​ ​get​ ​sore​ ​after​ ​a​ ​while?​ ​ ​ ​(See​ ​question​ ​1​ ​under​ ​the​ ​PRODUCTS​ ​section.)

Extension​ ​Questions​:​ ​ ​Please​ ​answer​ ​the​ ​following​ ​questions.

8. ​ ​What​ ​were​ ​the​ ​differences​ ​between​ ​your​ ​two​ ​experiments?​ ​ ​How​ ​did​ ​changing​ ​a​ ​variable​ ​(i.e.​ ​squeeze​ ​time,
rest​ ​time​ ​or​ ​some​ ​other​ ​variable​ ​you​ ​chose)​ ​lead​ ​to​ ​different​ ​results?​ ​ ​Discuss​ ​your​ ​findings​ ​here.

9. What​ ​would​ ​happen​ ​if​ ​you​ ​did​ ​not​ ​stop​ ​squeezing​ ​the​ ​tennis​ ​ball?​ ​ ​What​ ​do​ ​you​ ​think​ ​would​ ​happen​ ​to​ ​your
muscle​ ​cells​ ​and​ ​body​ ​as​ ​a​ ​whole?​ ​ ​Could​ ​you​ ​survive​ ​off​ ​of​ ​lactic​ ​acid​ ​fermentation​ ​alone?

10. Explain​ ​how​ ​the​ ​food​ ​you​ ​ate​ ​today​ ​contributed​ ​to​ ​the​ ​energy​ ​you​ ​used​ ​for​ ​this​ ​lab:

11. What​ ​did​ ​you​ ​learn​ ​today​ ​that​ ​you​ ​didn’t​ ​know​ ​before?

12. After​ ​today’s​ ​lesson​ ​on​ ​cellular​ ​respiration,​ ​what​ ​is​ ​still​ ​“cloudy”​ ​or​ ​difficult​ ​for​ ​you​ ​to​ ​understand?

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