Slit Skin Smear Test by Dalman Jhaziel
Slit Skin Smear Test by Dalman Jhaziel
Slit Skin Smear Test by Dalman Jhaziel
SLIT SKIN
SMEAR TEST
GROUP 1
BSN 1-YB-17
TODAY'S DISCUSSION
TOPIC OUTLINE
This photomicrograph
This photomicrograph reveals some of the
reveals some of the classic histopathologic changes in a
histopathologic changes specimen of human
found in a skin section from testicular tissue, which
an individual with a case of included a large number of
the leprosy. “foam cells”. These changes
were attributed to a case of
multibacillary (MB) leprosy.
II. DEFINITION: DESCRIPTION OF LAB DX PROCEDURES
HOW DOES A SLIT-SKIN SMEAR TEST
WORK? A skin smear is a test in which a sample
of material is collected from a tiny cut in
the skin and then stained for M. leprae,
an acid-fast bacillus.
This acid-fast-stained
This photomicrograph of a
photomicrograph of a tissue
skin tissue sample from a
sample extracted from a
patient with leprosy shows a
patient with leprosy shows a
cutaneous nerve, which had
chronic inflammatory lesion
been invaded by numerous
known as a granuloma,
M. leprae bacteria (shown in
within which numerous red-
red).
colored M. leprae bacteria are
visible.
SLIT SKIN SMEAR
III. MATERIALS (EQUIPMENT)
1 Wash your hands (1) and put on gloves. 6 Pinch the skin firmly between your
thumb and forefinger; maintain pressure
Take a new, clean, unscratched to press out the blood.
2
microscope slide. Using a slide marker, 7 Make an incision in the skin about 5 mm
write the patient identification (ID) long and 2 mm deep (3). Keep on
number at the bottom of the slide (2). pinching to make sure the cut remains
This number must be on the request bloodless. If bleeding, wipe the blood
form. away with cotton wad.
3 Clean the skin at the smear sites with a 8 Turn the scalpel 90° and hold it at a right
cotton wad drenched in alcohol. Let it angle to the cut. Scrape inside the cut
dry. once or twice with the side of the
scalpel, to collect tissue fluid and pulp.
4 Light the spirit burner.
There should be no blood in the
specimen, as this may interfere with
5 Put a new blade on the scalpel handle. If
staining and reading.
you put the scalpel down, make sure the
blade does not touch anything.
9 Stop pinching the skin and absorb any 14 Dress the wounds and thank the patient
bleeding with a wad of cotton.
10 Spread the material scraped from the Let the slide dry for 15 minutes at room
15
incision onto the slide, on the same side temperature, but not in direct sunlight.
as the ID number. Spread it evenly with
16 Fix the smears by passing the slide, with
the flat of the scalpel, making a circle 8
the smears upwards, slowly through the
mm in diameter (4).
flame of a spirit burner, 3 times (5). Do
11 Rub the scalpel with a cotton wad
not overheat. The slide should not be
drenched in alcohol. Pass the blade
too hot to touch.
through the flame of the spirit burner for
17 Put the slide in a slide box and send to
3 to 4 seconds. Let it cool without
the laboratory with the skin smear
touching anything.
request form.
12 Repeat the steps above for the second
site. Spread this smear next to, but not
touching, the first one.
SLIT SKIN
SMEAR TEST
Group Members:
Brinio, Zachary
Casinto, Czarina
Corpuz, Ian Christopher
Dabay, John Nicole
Dalman, Jhaziel
BSN 1-YB-17