A Rapid Test For Detection of Dengue Fever
A Rapid Test For Detection of Dengue Fever
A Rapid Test For Detection of Dengue Fever
DG-RD0102_2014-01
ASSAY PROCEDURE
C = Control line
These lines are not seen
T2 = IgM Test line
before the cassette is used.
T1 = IgG Test Line
Invalid: Control line (C) is absent. If this occurs, the assay should be
1. Bring test cassette and chase buffer to room temperature (if repeated using a new test cassette.
precipitates are noted in the chase buffer reagent, shake the bottle
vigorously and allow to warm up further). Positive results may appear as early as 5-10 minutes. Negative
2. Gently tear open the pouch and remove the test cassette. Lay the test results must be confirmed after 20 minutes.
device on a clean, flat work surface. The results are stable for up to 30 minutes. Do not read the results
3. Label the test cassette with the sample name. after 30 minutes.
4. Pipette 5 µl of serum, plasma, or whole blood into the upper portion of
the sample well (as marked in the image above). Make sure that there
are no air bubbles. EXPECTED VALUES:
5. Add 2 drops of buffers into the same sample well of the test device. Primary dengue is characterized by the presence of detectable IgM
Sample will start wicking up. antibodies 5 days after the onset of infection.
6. Read the test result within 20 minutes.
Secondary dengue is characterized by the elevation of specific IgM
antibodies and the elevation of specific IgG antibodies. Usually IgM
Negative results must be confirmed only after 20 minutes. Do not
antibodies will rise within 1-2 days after the onset of symptoms and IgG
read results after 30 minutes.
antibodies will be detectable after 20 days of infection.
REFERENCES
1. Sabin, AB and Schlesinger RW. Production of immunity to dengue
with virus modified by propagation in mice: Science (1945),
101:640.
2. Lam, SK. Dengue haemorrhagic fever. Rev. Med. Micro. (1995),
6:39-48.
3. Innis, BL, Nisalak, A., et.al. An enzyme-linked immunosorbent
assay to characterize dengue infections where dengue and
Negative Positive for Positive for Positive for Japanese encephalitis co-circulate. Am. J. Trap. Med. Hygiene
IgM IgG IgM & IgG (1989), 40:418-427.
4. CDC/NIH Guidelines. Biosafety in Microbiological and Biomedical.
5. Laboratories. 2nd Edition, 1988
1. Negative: 6. Siti-Strong. Diagnosis, prevention, and treatment of tropical disease,
Only control line (C) is visible. No IgG or IgM antibodies were detected. 7th ed., Philadelphia, The Ablakiston Company.
The result does not exclude dengue infection. If symptoms persist, a
new sample should be drawn from the patient in 3-5 days and then
should be retested (see the limitations section). ORDER INFORMATION
2. Positive for IgM: Product Code Description Packing Size
Coloured bands appear at the control line (C) and test line (T2). The
test is positive for IgM antibodies. This is indicative of a primary dengue RDG-RD0102 Dengue IgG/IgM Rapid 25 tests / kit
infection (see the limitations section).
3. Positive for IgM and IgG: MANUFACTURER
Coloured bands appear at the control line (C) and both test lines (T1 Reszon Diagnostics International Sdn. Bhd.
and T2). The test is positive for IgM and IgG antibodies. This is Revongen Corporation Center, No.12A, Jalan TP5, Taman Perindustrian
indicative of a secondary dengue infection (see the limitations section). UEP, 47600 Subang Jaya, Selangor, Malaysia
4. Positive for IgG: Tel : 603-8025 1603
Coloured bands appear at the control line (C) and test line (T1). The Fax : 603-8025 1637/1354
test is positive for IgG antibodies. This is indicative of a past dengue Email : info@reszonics.com
infection (see the limitations section). Website : www.reszonics.com
DG-RD0102_2014-01