Dionex Autotrace 280 Spe Instrument Operator'S Manual: Document No. 065330 Revision 05 March 2020
Dionex Autotrace 280 Spe Instrument Operator'S Manual: Document No. 065330 Revision 05 March 2020
Dionex Autotrace 280 Spe Instrument Operator'S Manual: Document No. 065330 Revision 05 March 2020
Operator’s Manual
1 • Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3 Safety Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.3.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.4 Regulatory Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4.1 Notice on Lifting and Handling of Thermo Scientific
Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
1.4.2 Notice on the Proper Use of Thermo Scientific Instruments . . . 7
1.4.3 Notice on the Susceptibility to Electromagnetic Transmission . 7
1.4.4 WEEE Compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2 • Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Operating Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Front Panel Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.1.2 Front Panel Display Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.1.3 Cartridges . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2 Left-Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3 Right-Side View . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.4 Fluid Schematic . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
2.5 Solid-Phase Extraction Process . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4 • Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.1 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
4.2 Dionex AutoTrace 280 Diagnostic Methods . . . . . . . . . . . . . . . . . . . . . 70
4.3 General Operation Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
4.4 Liquid Handling Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
5 • Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
5.1 Replacing Tubing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
5.2 Tightening the Nitrogen Manifold Fittings . . . . . . . . . . . . . . . . . . . . . .83
5.3 Replacing a Cartridge Holder O-Ring . . . . . . . . . . . . . . . . . . . . . . . . . .84
5.4 Replacing a Disk Holder O-Ring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85
5.5 Replacing the Liquid Handling Syringe . . . . . . . . . . . . . . . . . . . . . . . . .87
5.6 Replacing the Air Push Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
5.7 Converting a Cartridge Holder (Standard Dionex AutoTrace 280) . . . .96
5.8 Converting a Cartridge Holder (Dionex AutoTrace 280 PFAS) . . . . . .99
5.9 Cleaning a Sample Pump Cylinder and Piston . . . . . . . . . . . . . . . . . . .101
5.10 Calibrating a Sample Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .103
5.11 Replacing a Sample Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .104
5.12 Replacing the 12-Port Valve Rotor . . . . . . . . . . . . . . . . . . . . . . . . . . .107
5.13 Replacing the 12-Port Valve Stator . . . . . . . . . . . . . . . . . . . . . . . . . . .108
5.14 Replacing the 12-Port Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109
5.15 Replacing the Air Push Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112
5.16 Replacing the Switching Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .114
5.17 Running the Benchmark Test Diagnostic Method . . . . . . . . . . . . . . . .117
5.18 Aligning the Elution Nozzles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119
5.19 Replacing the Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . .121
A • Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
A.2 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
A.3 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
A.4 Gas Regulator and Gas Gauge Range . . . . . . . . . . . . . . . . . . . . . . . . . 124
A.5 Front Panel Display and Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
A.6 Liquid Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
A.7 Solid-Phase Extraction Configurations . . . . . . . . . . . . . . . . . . . . . . . . 124
A.8 Collection Container Racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
A.9 Sample Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 125
B • Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
B.2 Unpacking the Dionex AutoTrace 280 . . . . . . . . . . . . . . . . . . . . . . . . 127
B.3 Installation Instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
B.3.1 Connecting the Solvent Reservoirs . . . . . . . . . . . . . . . . . . . . 128
B.3.2 Installing the Waste Drain and Tubing . . . . . . . . . . . . . . . . . . 133
B.3.3 Connecting the Exhaust Hose . . . . . . . . . . . . . . . . . . . . . . . . . 137
B.3.4 Connecting the Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . 137
B.3.5 Checking the Compression Fittings . . . . . . . . . . . . . . . . . . . . 139
B.3.6 Installing the AutoTrace Software . . . . . . . . . . . . . . . . . . . . . 140
B.3.7 Selecting the Method Type . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
B.3.8 Connecting to the PC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 142
B.3.9 Connecting the Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . 143
D • Glossary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .151
Index
1.1 Overview
The Thermo Scientific™ Dionex™ AutoTrace™ 280 SPE Instrument (called Dionex
AutoTrace 280 for short) is an automated solid-phase extraction (SPE) system for
use with large samples (20 mL to 4 L) to isolate trace organics in water or aqueous
matrices. The compounds of interest are trapped on SPE adsorbents (cartridge or
disk format) and then eluted with strong solvents to generate an extract ready for
an analysis.
A modified version, the Thermo Scientific™ Dionex™ AutoTrace™ 280 PFAS, is
available for extraction of PFAS (per- and polyfluoroalkyl) from drinking water.
Unless otherwise stated, information in this manual applies to both the standard
and PFAS systems.
The Dionex AutoTrace 280 is available in both cartridge and disk models:
• Cartridge models use SPE devices in the cartridge format. Polymer cartridges
are available in the following sizes: 1 mL, 3 mL, and 6 mL.
Note that the 6 mL cartridge system is available in both Teflon and PEEK™
formats.
Glass cartridges are available in one size: 6 mL.
• Disk models use SPE devices in the disk format. Disks are available in one
size: 47 mm.
The Dionex AutoTrace 280 saves times, solvent, and labor, ensuring high
reproducibility and productivity for analytical laboratories. The instrument can
process up to six samples in 2 to 3 hours with only 15 minutes of operator
involvement. The Dionex AutoTrace 280 uses powerful pumps (with no check
valves) and proven constant flow technology to efficiently process even the most
difficult samples. With the Dionex AutoTrace 280 and a Thermo Scientific™
Dionex™ Accelerated Solvent Extraction (ASE™) system, laboratories can
effectively automate the solvent-extraction process for liquid and solid matrices.
The water samples are loaded or directed through the SPE cartridge or disk, using
a pump. (There is a separate pump for each of the six channels.) Loading is
performed in parallel, since this is the rate-limiting step of the SPE process.
Analytes are eluted from the SPE material one channel at a time, using positive
pressure. Positive pressure ensures more uniformity when loading or eluting,
which leads to better reproducibility. Effluent from the SPE adsorbents can be
collected in six different elution containers (11 mm GC vials, 15 mL conical
tubes, 16 x 100 mm test tubes, 17 x 60 mm vials, 4 mL screw cap vials, or 40 mL
collection vials).
AutoTrace Software
AutoTrace software version 1.0.0 (or later) is provided with the system. The
software can be used to create methods that automate the following SPE
processes:
• Conditioning the cartridge or disk
• Loading sample onto the cartridge or disk
• Rinsing the cartridge or disk
• Drying the cartridge or disk
• Eluting the sample
The AutoTrace software must be installed on a PC running either Microsoft
Windows10 Pro or Windows 8.1 Pro. For communication between the Dionex
AutoTrace 280 and the PC, the Dionex AutoTrace 280 must be connected to a
USB (Universal Serial Bus) 2.0 port on the PC.
NOTE If you connect the USB cable to a USB 3.0 port, the
system will not operate correctly.
Signale une situation de danger immédiat qui, si elle n'est pas évitée,
entraînera des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures mineures à modérées. Également
utilisé pour signaler une situation ou une pratique qui pourrait
gravement endommager l'instrument mais qui n'entraînera pas de
blessures.
Warnhinweise in Deutsch
Notes
This symbol indicates that the equipment must not be thrown into general
waste and should be collected separately and processed in accordance
with local and state requirements.
Conformité DEEE
Ce symbole indique que l'équipement ne doit pas être jeté avec les déchets
ordinaires, mais doit être collecté séparément et traité conformément aux
règlementations locales et nationales.
WEEE Konformität
Instrumente mit diesem Zeichen sind nicht für den normallen Abfall
bestimmt; Entsorgung soll den lokalen Vorschriften entsprechend
ausgeführt werden.
4
Figure 2-1. Dionex AutoTrace 280 Front View (Cartridge Model)
# Part Function
1 Sample Pumps Pumps sample from sample containers to switching
valves.
2 Cartridge/Disk Holders Holds the SPE cartridges or disks (depending on the
Dionex AutoTrace 280 model) for the extraction
process.
# Part Function
3 Elution Station Collects aqueous waste, solvent waste, or sample
effluent on the elution station (see Figure 2-4).
4 Front Panel Communicates system status to the user.
The keypad offers immediate control of the most frequently used Dionex
AutoTrace 280 functions:
Button Function
LOAD Increments the method number displayed on
the front panel.
PAUSE • Pressing PAUSE while the system is
idle displays the START screen.
• Pressing PAUSE while a method is
running suspends operation (after the
step in process is completed).
CONT • Pressing CONT resumes operation after
the user presses PAUSE, responds to
an error condition, or performs some
type of manual intervention (rinses the
sample container, for example).
• Pressing CONT once selects the
method number currently displayed;
pressing CONT a second time starts the
selected method.
PAUSE + CONT Pressing the PAUSE and CONT buttons
simultaneously aborts the method currently
running.
AutoTrace 280
SPE Instrument
Firmware Version
v01.00.00
When the START screen is displayed, you can press LOAD to select the
required method. There are three types of methods:
• Methods 1 through 24 are operational methods created by the user in
the AutoTrace software and then downloaded from the PC to the
Dionex AutoTrace 280. For details about creating methods, refer to
the AutoTrace software Help.
• Methods 25 through 31 are diagnostic methods (preprogrammed by
Thermo Fisher Scientific) that can be selected and run from the
Dionex AutoTrace 280 front panel. For details about when to run the
diagnostic methods, see Section 4.2.
• Methods 32 through 34 are reserved for use by Thermo Fisher
Scientific Manufacturing personnel.
2.1.3 Cartridges
The Dionex AutoTrace 280 uses silica-based Thermo Scientific™
Dionex™ SolEx® Solid-Phase Extraction (SPE) Cartridges. The cartridges
are available in C8, C18, graphite, and polymeric functionalities. The C18
material is available as endcapped or nonendcapped silica to provide the
selectivity required by various applications.
The 1 mL cartridges contain 0.1 g of packing, the 3 mL cartridges contain
0.5 g of packing, and the 6 mL cartridges contain 1 g of packing. For a list
of Dionex SolEx cartridges available for use with the system, see
Appendix C.
11
12 10
9
1
2
8
3
4 5 6 7
# Part Function
1 Solvent Ports (Behind door, not shown) Input ports for five
solvents.
2 Waste Port (Behind door, not shown) Output port for system
waste from purging solvent lines and/or washing the
liquid handling syringe.
3 Gas Inlet Input for the clean gas supply.
# Part Function
4 Air Push Syringe Draws in and delivers air that follows solvent
transfer (to ensure that transfer is complete).
5 Liquid Handling Draws in and dispenses solvent through the system.
Syringe
6 Gas Regulator Knob Regulates the gas pressure in the system that is used
for drying cartridges or disks and for sample
concentration.
7 Gas Pressure Gauge Indicates the gas pressure set by the knob.
8 Elution Rack Holds tubes or vials for collection of sample effluent.
9 Elution Station Directs effluent to one of two waste positions, or
collects effluent in the collection container by
moving it into the appropriate position.
10 12-Port Valve Controls the flow of solvent to whatever position is
specified in the method step.
11 Switching Valves Directs the flow of gas, solvent, or sample to the
cartridge or disk holders.
12 Air Push Valve (3-Way Draws in or vents air for the air push syringe.
Valve)
Aqueous Waste Tubing Routes sample and aqueous effluent to waste.
(Not shown)
Solvent Waste Tubing Routes solvent effluent to waste.
(Not shown)
5 4
# Part Function
1 Sample Input Lines Connects the system to sample containers.
2 Exhaust Port Outlet for routing solvent vapors to a suitable vent
location.
3 USB Port Connects the USB cable from the Dionex
AutoTrace 280 to a USB 2.0 port on the PC on
which the AutoTrace software is installed.
Note: If you connect the USB cable to a USB 3.0
port, the system will not operate correctly.
# Part Function
4 Fuse Holder, Power • The fuse holder contains two fast-blow IEC
Switch, and Power 127 fuses rated 3.15 A (P/N 954745). For
Receptacle instructions on how to change the fuses, see
Section 5.19.
• The power switch provides on/off control of
power to the Dionex AutoTrace 280.
• The power cord plugs into the IEC 320 three-
prong receptacle.
5 Model Data Label The model data label lists fuse and power
information, as well as the Dionex AutoTrace 280
serial number. You will be asked to provide the
serial number when ordering replacement parts.
The power supply cord is used as the main disconnect device. Make
sure the socket-outlet is located near the Dionex AutoTrace 280 and is
easily accessible.
Pump
#1
SPE
Cartridge
or Disk #1
Switching
Valve #1
Incoming
Sample
#1
To Valves
Regulator 2-6
Gas Nitrogen Manifold
12-Port To Valves
Air Valve
Air 2-6
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Solvent 5
Waste
Air Syringe Liquid
Handling
Syringe
Start
SPE method
12-port valve
selects one of five
solvents
Continued...
=Solvent
=Air
Pump =Gas
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous Solvent Collect
Waste Waste
Regulator
Gas Nitrogen Manifold
12-Port
Air Air Valve
Vent Valve
Volume according
to Air Factor setting
Volume according
to solvent volume
for condition step Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Continued...
12-port valve
switches to the
switching valve
Liquid handling
syringe dispenses
conditioning solvent
Continued...
Figure 2-9. Condition Cartridge: Liquid Syringe Dispenses Solvent Flow Chart
=Solvent
=Air
Pump =Gas
#1
SPE
Cartridge #1 Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste or Solvent
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Air Air Valve
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-10. Condition Cartridge: Liquid Syringe Dispenses Solvent Diagram
Continued...
Continued...
=Solvent
=Air
Pump =Gas
#1
Regulator
Gas Nitrogen Manifold
12-Port
Air Air
Valve
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-12. Condition Cartridge: Air Push with Air Factor of 1.0 Diagram
=Solvent
=Air
Pump =Gas
#1
SPE
Cartridge #1 Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste or Solvent
Waste
Solvent stops here
with 0.5 Air Factor
Regulator
Gas Nitrogen Manifold
12-Port
Air Air Valve
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Liquid Solvent 4
Air Syringe Solvent 5
Handling Waste
Syringe
Figure 2-13. Condition Cartridge: Air Push with Air Factor of 0.5 Diagram
Continued...
Continued...
=Sample
=Gas
Pump
#1
SPE
Cartridge #1 Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air
Air Valve
Vent
Solvent 1
Solvent 2
Solvent 3
Air Syringe Liquid Solvent 4
Solvent 5
Handling Waste
Syringe
Figure 2-15. Load Sample Diagram
Continued...
Continued...
Figure 2-16. Cartridge Rinse: Liquid Handling and Air Push Syringes Fill Flow Chart
=Solvent
=Air
=Sample
Pump =Gas
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste Solvent Collect
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Air Valve
Vent Air
Valve
Volume according
to internal setting
Volume according
to solvent volume
for rinse step Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-17. Cartridge Rinse: Liquid Handling and Air Push Syringes Fill Diagram
Continued...
12-port valve
switches to the
switching valve
Liquid handling
syringe dispenses
rinse solvent
Continued...
Figure 2-18. Cartridge Rinse Liquid Handling Syringe Dispenses Solvent Flow Chart
=Solvent
=Air
=Gas
Pump
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste or Solvent
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air Air
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-19. Cartridge Rinse: Liquid Handling Syringe Dispenses Solvent Diagram
Continued...
Continued...
=Air
=Gas
Pump
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste or Solvent
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air Air
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-21. Cartridge Rinse: Air Push Diagram
Continued...
Switching valve
selects gas port
Continued...
=Gas
Pump
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste or Solvent
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air Air
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-23. Dry with Gas Diagram
Continued...
Continued...
Figure 2-24. Elute to Collect: Liquid Handling and Air Push Syringes Fill Flow Chart
=Solvent
=Air
=Gas
Pump
#1
SPE
Cartridge #1
Switching
Valve #1
Incoming
Sample
#1
Aqueous
Waste Solvent Collect
Waste
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air Air
Vent Valve
Volume according
to internal setting
Volume according
to solvent volume
for elution step Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-25. Elute to Collect: Liquid Handling and Air Push Syringes Fill Diagram
Continued...
Continued...
Figure 2-26. Elute to Collect: Liquid Handling Syringe Dispenses Solvent Flow Chart
=Solvent
=Air
Pump =Gas
#1
SPE Switching
Cartridge #1
Valve #1
Incoming
Sample
#1
Collect
Regulator
Gas Nitrogen Manifold
12-Port
Air Valve
Air
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-27. Elute to Collect: Liquid Handling Syringe Dispenses Solvent Diagram
Continued...
=Air
=Gas
Pump
#1
SPE
Cartridge #1 Switching
Valve #1
Incoming
Sample
#1
Collect
Regulator
Gas Nitrogen Manifold
12-Port
Valve
Air Air
Vent Valve
Solvent 1
Solvent 2
Solvent 3
Solvent 4
Air Syringe Liquid Solvent 5
Handling Waste
Syringe
Figure 2-29. Elute to Collect: Air Push Diagram
Operation Description
Conditioning the Use Condition Cartridge or Condition Disk to pass
cartridge or disk the solvent through the cartridge or disk, respectively,
and direct the effluent to waste.
Loading the sample Use Load Sample to pump sample from the sample
container to the cartridge or disk for extraction.
Rinsing the cartridge Use Cartridge Rinse or Disk Rinse after loading the
or disk sample path and the cartridge or disk, respectively.
The effluent is directed to waste.
Washing the syringe Use Wash Syringe to prewash the liquid handling
solvent syringe with the selected solvent. The resulting
solvent waste exits through the waste port.
Eluting the sample Use Elute to Collect to pass solvent through the
cartridge or disk and direct the eluent into the
collection container.
Operation Description
Rinsing the sample Use Rinse Sample Container to manually add
container solvent and swirl to rinse the sample container walls
before continuing.
Drying with gas Use Dry with Gas to flow gas through the cartridge
or disk, clearing the path of residual water.
Pausing operation Use Pausing Operation to delay operation or to stop
operation and signal manual intervention.
Increasing absorption Use Elute to Soak before an Elute to Collect step to
time allow solvent to soak the cartridge or disk.
Cleaning the sample Use Clean Sample Path method steps between
path sample batches to prevent cross-contamination.
Concentrating the Use Concentrate Sample for automated
sample concentration of the eluent.
This section describes the steps involved in operating the Dionex AutoTrace 280:
• Selecting the Dionex AutoTrace 280 method type (see Section 3.1)
• Creating a method (see Section 3.2 and Section 3.3)
• Loading a method (see Section 3.4)
• Preparing the Dionex AutoTrace 280 for operation (see Section 3.5)
• Running a method (see Section 3.6)
• Post-run activities (see Section 3.7)
If... Then...
You need to improve recovery Decrease the flow rate(s)
You need to improve throughput Increase the flow rate(s)
The liquid handling syringe stalls Decrease the flow rate(s)
You are using viscous liquids Adjust the Push Delay
You need to decrease the amount of air Reduce the Air Factora
between multiple conditioning steps
Table 3-2. Setup Parameters
a. When using an Air Factor of less than 1, always perform a Cartridge
Rinse (or Disk Rinse) after loading the sample.
If... Then...
The sample contains volatile compounds Add a Pause and Alert step
between the conditioning and
loading steps (to ensure that
samples are not introduced
before the system is ready)
The manual method requires a “rinse with Use a Condition Cartridge (or
water” step without allowing the water to Condition Disk) step rather than
leave the cartridge or disk a Cartridge Rinse (or Disk
Rinse) step, so that a smaller air
push is used
You need an extended contact time Introduce a Timed Pause step
between solvent and packing material
The sample contains a large amount of Add 5% to 7% to your volume
solids entry for the Load Sample step
–or–
Filter the sample, collect the
filtrate, and place this in the
sample container
Table 3-3. Method Step Considerations
If... Then...
You are going between immiscible Perform a Wash Syringe step
solvents in the method with an intermediate solvent
before performing the next step
(even if Autowash is being
used)
You need syringe washing to appear in Disable Autowash and use
your method printout for auditing Wash Syringe method steps
purposes where needed
You are running with the Air Factor Perform a Cartridge Rinse (or
parameter set to less than one Disk Rinse) step between
sample loading and elution
The elution solvent is immiscible in water Perform a Dry with Gas step to
remove all water from the
cartridges or disks and eliminate
channeling
You are not drying the cartridge or disk Perform a Cartridge Rinse (or
after loading sample Disk Rinse) step before elution
There is water in your extract Increase the pressure and/or time
of the Dry with Gas step
The elution solvent is not reaching the Use a volume of 3 mL or greater
packing material in the first Elute to in the elution step
Soak step
You need to increase the contact time Use an Elute to Soak step
between solvent and packing material for before Elute to Collect
extraction of analytes
You need to minimize the amount of air Use an Elute to Soak step
introduced between multiple elution steps before Elute to Collect
You need to collect more than the Use an Elute to Collect, 2nd
maximum elution volume of your Tube step to double the
collection container collection volume
You need to improve throughput Decrease times wherever
possible
You need to improve recovery See Table 3-1
If... Then...
You need to run a solvent through the Use a Clean Sample Path step
sample path (from sinkers, through the to designate either of the waste
sample lines, sample pumps, switching positions
valves, and cartridges or disks, to a –or–
designated collection position) Use a Rinse Sample Container
step to designate either of the
For example, in a conditioning step waste positions or the collection
requiring 40 mL of water, rather than container position
using four Condition Cartridge (or
Condition Disk) steps of 10 mL each,
you can save time by using one 40 mL
Clean Sample Path step to aqueous
waste. Follow this with a Pause and
Alert step for moving the lines from the
water container to the sample containers.
Table 3-3. Method Step Considerations (Continued)
2. On the Set Up Methods window toolbar, click Load. The Find Method
window appears. The window contains a list of all the methods that were
previously defined and saved.
3. Double-click the name of the method you want to load.
4. Click Close to return to the Set Up Methods window. The settings for the
selected method are loaded into the right pane of the Set Up Methods window.
cartridge holder O-ring due to the presence of packing debris on the inside
walls of the SPE cartridge.
12. For the Dionex AutoTrace 280 cartridge model:
a. Place cartridges in each holder to be used by sliding a cartridge upward
onto the bottom of the cartridge holder's plunger.
b. Push down on the lever until the assembly “clicks” into place and the
holder's LED comes on (see Figure 3-1).
LED
Lever
Plunger
Cartridge
LED
Lever
Clip
Locking Collar
The display also reports when manual intervention is required and when the
sample run is finished.
Some method steps cause the system to pause operation and wait for manual
intervention. When this happens, the system beeps (if the Beeper On check box in
the Setup Parameters dialog box is selected) and the front panel displays a
message indicating the required action. Table 3-1 lists these front panel messages.
When you complete the manual steps, press CONT to have the system continue
running the method.
Lever
Clip
Never leave the cartridge holders in the down position without the
cartridges in place; this can deform the O-ring.
Lever
Clip
Locking Collar
5. When the system pauses the first time, insert the sample lines into a
clean container filled with a solvent.
Do not use acetone as a solvent to clean the sample path; it is harmful
to the sample pumps.
1. Fill the solvent reservoirs with the solvent of choice for flushing.
2. Check that the tubing is fully submerged in the solvent.
3. Swirl the solvent in the containers to rinse the outside surface of the
tubing and weights.
4. Select the Prime Solvents diagnostic method:
a. Press LOAD to display the LOADING METHOD screen.
b. Press LOAD to increment the displayed method to 29.
5. Press CONT to run the method. The method draws enough solvent
from each port to prime the liquid lines.
6. When the system pauses, press CONT.
Do not run pumps dry. Always leave deionized water in the sample
lines when the system is not in use.
• Clean the cartridge or disk holders, racks, and other system surfaces
with warm, soapy water.
• Check that all syringe and valve fittings are finger-tight.
• Check the O-rings in the bottom of the cartridge holders for cracking
or wear and replace as needed (see Section 5.3).
• Check overall system performance by running the Benchmark Test
diagnostic method (see Section 5.17). The Benchmark Test checks
the operation of the front panel, elution station, syringes, pumps, and
valves.
This chapter is a guide to troubleshooting minor problems that may occur while
operating the Dionex AutoTrace 280:
• Section 4.1 describes error messages and how to troubleshoot them.
• Section 4.2 describes the Dionex AutoTrace 280 diagnostic methods.
• The remaining sections in this chapter describe routine operating problems
and how to resolve them.
If you are unable to resolve a problem by following the instructions here, contact
Technical Support for Dionex products:
• In the U.S. and Canada, call 1-800-532-4752 and select option 2.
• Outside the U.S. and Canada, call the nearest Thermo Fisher Scientific office.
Please have this chapter at hand when talking with Technical Support personnel.
Pump Errors
Pump x stalled
Cause: Flow rate is too high for the cartridge or disk installed.
Action: Reduce the flow rate of the sample pump.
Cause: Sample may contain particles that are clogging the cartridge or disk.
Action: Filter the sample as required. Be careful not to lose analytes that
would affect recovery; for tips on optimizing sample recovery, see
Section 3.3.1.
Cause: High backpressure due to a tubing blockage.
Action: Replace blocked tubing with tubing of the same length and ID (see
Section 5.1).
Cause: Loose connection between the pump motor and the sensor connector.
Action: Reseat the pump connector.
Cause: The pump motor or sensor has failed.
Action: Replace the pump (P/N 071529 for the cartridge model; P/N 071530
for the disk model) (see Section 5.11).
Action: If the error message appears again, contact Technical Support for
Dionex products.
Syringe Errors
Valve Errors
Cause: Loose connection between the valve and the Valve Distribution PC
board.
Action: Reseat the valve connector.
Cause: Valve rotor is binding.
Action: Replace the rotor and stator (see Section 5.12 and Section 5.13).
Cause: Malfunction of the motor drive and/or electronics.
Action: Contact Technical Support for Dionex products.
4b. Valve fitting is loose. 4b. Make sure valve fittings are
securely finger-tight. If
tightening a fitting does not stop
a leak, replace the fitting (see
Section 5.1).
5. Liquid volumes 5a. Flow rates are too high, 5a. Reduce the flow rates.
delivered are incorrect. causing gas bubbles to form.
This chapter describes Dionex AutoTrace 280 service and repair procedures that
users can perform. All procedures not included here, including electronics-related
repair procedures, must be performed by Thermo Fisher Scientific personnel. For
assistance, contact Technical Support for Dionex products:
• In the U.S. and Canada, call 1-800-532-4752 and select option 2.
• Outside the U.S. and Canada, call the nearest Thermo Fisher Scientific office.
Please have the following information at hand when talking with the Technical
Support Representative:
• Dionex AutoTrace 280 serial number (printed on the model data label)
• AutoTrace software version (select Help > About to display the version)
Before replacing any part, see the troubleshooting information in Chapter 4 to
correctly identify the cause of the problem.
Substituting non-Dionex/Thermo Fisher Scientific parts may impair
the performance of the Dionex AutoTrace 280, thereby voiding the
product warranty. For details, see the warranty statement in the
Dionex Terms and Conditions.
For your reference, the Dionex AutoTrace 280 is plumbed with the tubing listed in
Table 5-3.
2. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
3. Remove the fittings at both ends of the tubing assembly to be replaced. As
you do, note the order and orientation of the fitting components. Also note the
tubing marker at each end of the tubing assembly.
4. (Optional) If the tubing assembly is connected to a switching valve, remove
the switching valve from the left-side panel to improve access to the fittings.
5. Remove the tubing assembly from any tubing clips.
6. Route the new tubing assembly through the same clips as the old tubing
assembly.
7. Install the new fittings and tubing markers in the same order and orientation
as the parts they are replacing. Tip: When installing the fittings, you may
want to use a small piece of emery cloth or fine sandpaper to hold the tubing
in place.
8. Install the fittings into the ports of the component. Reinstall the component, if
it was removed for easier access. Tighten fittings securely to prevent leaks.
Tip: If necessary, use latex gloves to obtain a tight grip.
9. If you removed the Dionex AutoTrace 280 rear panel, reinstall the rear panel
with the six screws previously removed.
10. Reconnect the power cord and turn on the power.
11. Place solvent lines in the solvent reservoirs.
12. Run the Benchmark Test diagnostic method (#30) (see Section 5.17).
2. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
Parts Needed
• Lint-free laboratory tissue
• Cartridge holder O-ring (see Appendix C for part numbers)
Parts Needed
• Disk Holder O-Ring Kit (P/N 071059)
Locking
Collar
Snap Ring Washer O-Ring
7. Remove the O-ring from the top part of the disk holder (see Figure 5-1):
a. Turn the top part of the disk holder upside down.
b. Rest one thumb and finger on the O-ring at opposite sides of the disk
holder. Press firmly on the O-ring.
c. While applying steady pressure to the O-ring, begin sliding your thumb
and finger around the O-ring. The pressure will cause the O-ring to pop
out of the groove slightly; when this happens, slide the tip of your index
finger under the O-ring and remove it from the groove.
Parts Needed
• Liquid handling syringe, 10 mL (P/N 070579)
Screw Housing
Black Thumbscrew
Plunger
Driving Arm
Thumbscrew
2. Remove the black thumbscrew from the bottom of the syringe plunger.
3. Gently slide the syringe assembly out of the housing.
4. Loosen the small black thumbscrew on the mounting bracket.
5. While holding the black mounting bracket in your left hand and the syringe in
your right hand, turn the syringe counterclockwise until it disengages from the
fitting.
6. Separate the syringe from the fitting.
Fitting
Plunger
Driving Arm Knurled
Thumbscrew
Thumbwheel
Top View
Luer-Lock Fitting
Knurled Knob
Chrome
End Piece Housing Tab
Syringe Clamp
Syringe Plunger
Driving Arm
Thumbscrew
2. Remove the black thumbscrew from the bottom of the syringe plunger.
3. Gently slide the syringe assembly out of the housing.
4. While holding the luer-lock fitting in your left hand and the syringe in your
right hand, turn the syringe counterclockwise until it disengages from the
fitting.
Plunger
Knurled Thumbscrew
Thumbwheel
4. Turn the thumbwheel to the right just until the clicking sound stops. Then,
turn it one more partial turn (see Figure 5-7).
Top View
Thumbwheel
Turn thumbwheel one more
partial turn to the right by
the amount shown.
Figure 5-7. Thumbwheel Rotation
Parts Needed
• Obtain the appropriate plunger assembly for the new cartridge size. For
descriptions and part numbers, see Appendix C.
LED
PTFE
Tubing
Tubing Clamp
Stainless
Tubing
Plunger
Assembly
3. Grasp the bottom of the PTFE tubing and the tubing clamp, and then carefully
pull upward until they slide off the top end of the stainless tubing.
4. Disengage the plunger clamp, and then move the plunger up and out of the
cartridge.
5. Unscrew the plunger assembly (see Figure 5-8) and pull it down to remove it
from the cartridge holder.
6. Set the plunger assembly aside.
7. Repeat Step 1 through Step 6 for each cartridge holder you need to convert.
3. Carefully, without developing kinks in the tubing, push the PTFE tubing end
downward, over the top of the stainless tubing, as shown in Figure 5-9.
PTFE Sleeve
PTFE Tubing
Stainless Tubing
5. Unscrew the plunger assembly (see Figure 5-11). Pull down on the assembly
to remove it from the cartridge holder.
6. Set the plunger assembly aside.
7. Repeat Step 1 through Step 6 for each cartridge holder you need to convert.
View A View B
5 4
6 3
4
5
# Part
1 Tubing assembly (including 1/4-28 PEEK nut and ferrule)
2 Coupler
3 Double-cone ferrule (Not shown in View A)
4 Split-cone fitting
5 Stainless steel tubing
6 Plunger assembly
3. For the Dionex AutoTrace 280 disk model: Remove the pulse damper from
the pump outlet.
4. Use the #2 Phillips screwdriver to remove the two Phillips screws on the front
of the pump. Remove the black cover.
5. Remove the white plastic pump head by pulling it straight away from the
pump.
6. Unscrew the metal retainer from the pump head. Tip: If necessary, use pliers
to loosen the retainer.
Note the order of the parts inside the retainer: The white washer is closest to
the retainer; the two red seals are closest to the pump head.
7. Rinse the bore in the pump head, the washer, and both seals with deionized
water. Be careful not to scratch the ID of the pump head bore; scratches will
prevent a proper seal.
8. Remove the piston from the pump by angling the piston and pulling it out.
9. Rinse the piston with deionized water. Be careful not to scratch the piston;
scratches will prevent a proper seal.
10. Place the metal retainer onto the piston, followed by the white washer and the
two red seals. The red seals must be placed onto the piston carefully, so as not
to deform them as they pass across the notched cutout of the piston. Install the
seals in the retainer in the order in which they were removed.
11. Reinstall the piston/retainer/washer/seal assembly by inserting the dowel pin
at the end of the piston into the hole in the bearing inside the pump drive bore.
12. Screw the pump head carefully onto the piston/retainer/washer/seal assembly.
Tighten the retainer. Using pliers, turn the retainer 10 degrees past finger-
tight.
13. Replace the black cover and reinstall the two Phillips screws on the front of
the pump.
14. Replace the compression fittings and the inlet and outlet tubing.
15. For the Dionex AutoTrace 280 disk model: Reinstall the pulse damper.
16. To verify that the pump is running correctly, run the Calibrate Pump
diagnostic method (#31). While the method runs, check for leaks from the
pump and pump volume accuracy.
Calibration Screw
Getting Started
Before removing the pump, follow the steps below to create and run a method that
will dry the line that runs from the pump to the switching valve. This prevents
leaking during the pump replacement.
1. In the AutoTrace software main window, click Methods. The Set Up Methods
window opens.
2. On the Set Up Methods window toolbar, click New.
3. In the Name box, enter Dry Lines as the method name.
4. Click the Edit button.
5. Under AutoTrace Method Step, expand the Solid-Phase Extract option to
display the SPE commands.
6. Double-click the Load Sample command and set the volume to 10.0 mL.
7. Double-click the Dry with Gas command and set the time to 0.5 min.
8. To save the method, click Save on the toolbar.
9. On the toolbar, click the down arrow next to Command and select Download
Method.
10. If the Set Up Methods window is not currently open, the Find Method
window opens. Double-click Dry Lines, the name of the method you want to
download.
11. When asked to confirm that you want to download the method to the Dionex
AutoTrace 280, click Yes.
12. Place the sample lines in an empty, clean container.
13. Run the Dry Lines method.
14. When the method has finished running, turn off the Dionex AutoTrace 280
power switch and disconnect the power cord from both its source and from
the Dionex AutoTrace 280 right-side panel.
Compression
Screws
Tubing
2. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
3. Locate the back of the defective pump and follow the pump’s wires to the
CPU board. Disconnect the pump from the board.
4. Remove the two screws that secure the pump to the pump mounting bracket.
Set the screws aside.
5. Carefully pull the pump away from the mounting bracket.
NOTE Do not run the 12-Port Valve Test unless all solvent lines
have been primed.
5. Open the left-side door. Disconnect all of the fittings on the 12-port valve
stator. If a fitting is too tight to be removed, loosen it with a 1/4-in wrench.
6. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
7. Remove the electrical cable from the bottom of the 12-port valve.
8. Note the four Phillips screws next to the valve stator. (The screws are on the
outside, near the face of the 12-port valve.) While holding the valve in place,
remove the four screws.
NOTE If you do not support the valve, it will fall down inside
the Dionex AutoTrace 280 when you are undoing the last
screw.
2. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
3. Remove the three white compression
fittings on the air push valve (see
Figure 5-15).
Compression
Fitting (3)
4. Locate and disconnect the valve wiring connector from the Valve Distribution
PC board (see Figure 5-16).
Alignment
Notch
5. Using the #2 Phillips screwdriver, remove the six Phillips screws securing the
rear panel to the system. Set the panel and screws aside.
6. Locate the connector for the defective valve on the Valve Distribution PC
board (see Figure 5-19). Unplug the connector.
7. Follow the group of green wires and carefully undo the wires from the wire
fasteners.
8. From the left side of the Dionex AutoTrace 280, locate the mounting bracket
that holds the defective valve to the side panel.
9. Remove the two screws that hold the mounting bracket to the side panel. Set
the screws aside.
10. Route the defective valve wires from the inside of the system through the
hole.
11. Remove the valve from the Dionex AutoTrace 280.
12. Remove the two screws in the valve to separate the valve from its bracket.
Parts Needed
• Six 16 x 100 mm test tubes and elution rack
–or–
For alignment of the 40 mL elution rack only: Six 40 mL collection vials and
elution rack
• Sample container with at least 100 mL of water
• Solvent containers filled with colored water
Tools Needed
• Small pair of pliers
Elution Nozzle
Vial #6
5. If a nozzle contacts the side of the vial, use a small pair of pliers to adjust the
nozzle toward the center of the vial. Figure 5-21 shows an example of a
nozzle that is aligned.
Elution Nozzle
6. Continue running the Benchmark Test. Verify that each nozzle delivers
solvent into the vial without spilling.
4. Insert two new 3.15 amp IEC127 fast-blow fuses into the springs in the fuse
drawer. Press gently to fully insert the fuses into the drawer.
5. Insert the fuse drawer into the right-side panel and press until the drawer
snaps into place.
6. Reconnect the power cord and turn on the power switch.
7. Verify that the READY light comes on and the system operates normally.
A.1 Electrical
Power 100 to 240 Vac (±10%), 47 to 63 Hz. The Dionex AutoTrace 280
Recommendations main power supply is auto-sensing and requires no voltage or
frequency adjustment.
Typical Input 100 W
Power
Maximum Line 1.2 A
Draw
Fuse Two fast-blow IEC 127 fuses (P/N 954745) rated 3.15 A
Requirements
A.2 Environmental
Operating 10 to 40 C (50 to 104 F)
Temperature Note: The Dionex AutoTrace 280 is intended for operation
indoors only.
Operating 20% to 80% relative humidity (noncondensing)
Humidity
Operating Altitude -61 to 2000 meters (-200 to 6562 ft)
A.3 Physical
Dimensions 69 × 57 × 63.5 cm (27 × 23 × 25 in)
(h × w × d) Sample rack: 42.16 × 33.02 cm (16.6 × 13.0 in)
Do not run the sample pumps dry. Always leave deionized water in the
sample lines when the system is not in use.
Lift the Dionex AutoTrace 280 only from the bottom and/or sides of the
instrument. Use caution when lifting the Dionex AutoTrace 280: it
weighs 43.09 kg (95 lb).
Wenn Sie den Dionex AutoTrace 280 anheben oder bewegen möchten,
greifen Sie bitte unter den Boden oder heben Sie das Gerät an den
Seiten an. Seien Sie vorsichtig, wenn Sie den Dionex AutoTrace 280
anheben. Das Gerät wiegt 43.09 kg.
1. Open the Dionex AutoTrace 280 shipping container. Remove the Dionex
AutoTrace 280 and place it on the workbench.
2. Unpack the Ship Kit (standard Dionex AutoTrace 280, P/N 071383; Dionex
AutoTrace 280 PFAS, P/N 22136-62005) and all other items included in the
shipping container. Place the items in a convenient location and check them
against the packing list. If there are any discrepancies, immediately report
them to Thermo Fisher Scientific.
To connect solvent tubing lines on the left side of the system to the
solvent reservoirs:
1. Position the reservoirs to the left of the standard Dionex AutoTrace
280, keeping the following considerations in mind:
• The reservoirs must be within reach of the tubing that extends
from the left side of the system.
2. Uncap the solvent reservoirs and fill them with the required solvents.
3. Locate the tubing weights (P/N 071360) and barbed fittings
(P/N 070357) on the instrument assembly.
4. Insert the end of the tubing from solvent port #1 through the hole in
one of the reservoir caps. Cut the tubing length, if necessary, to avoid
slack or kinks in the solvent lines.
5. Push the tubing end through the small hole in the top of one of the
tubing weights until the tubing extends through the opposite end (see
Figure B-1).
Small Hole
Tubing Weight
6. Push a barbed fitting into the end of the tubing (see Figure B-2).
Barbed
Fitting
Figure B-2. Barbed Fitting
To hold the tubing in your fingers while installing the barbed fitting,
grasp the tubing with a flat rubber band or a piece of emery cloth.
7. Gently pull the tubing upward until the barbed fitting seats itself
within the recess of the tubing weight (see Figure B-3).
Pull upward
Recessed Area
Figure B-3. Pulling Tubing Upward
8. Cap the bottle and adjust the length of tubing until the weight touches
the bottom surface of the reservoir (see Figure B-4).
Tubing Weight
Figure B-4. Tubing Weight in Reservoir
To connect the solvent tubing lines on the left side of the system to the
solvent reservoirs:
1. Position the reservoirs to the left of the Dionex AutoTrace 280 PFAS,
keeping the following considerations in mind:
• To prevent contamination, wear cleanroom gloves (lint-free,
particle-free, and oil-free) when handling tubing or making
tubing connections.
• The reservoirs must be within reach of the tubing that extends
from the left side of the system.
• The most precise liquid transfer occurs when the solvent
reservoirs are positioned slightly higher than the system's 12-port
valve. Thermo Fisher Scientific recommends that you do not
place the solvent reservoirs on the top of the system.
2. Uncap the solvent reservoirs and fill them with the required solvents.
3. Locate the tubing weights (P/N 22163-20003), 1/4-28 PEEK nuts
(P/N 00101-07-00027), and ferrules (P/N 00101-18017) provided in
the Dionex AutoTrace 280 PFAS Ship Kit (P/N 22136-62005).
4. Insert the end of the tubing from solvent port #1 through the hole in
one of the reservoir caps. Cut the tubing length, if necessary, to avoid
slack or kinks in the solvent lines.
5. Install a 1/4-28 PEEK nut and a ferrule on one end of the tubing.
6. Secure the 1/4-28 PEEK nut at the end of the tubing to the tubing
weight.
Tubing
Tubing Weight
7. Cap the bottle and adjust the length of tubing until the weight touches
the bottom surface of the reservoir (see Figure B-4).
Tubing Weight
Figure B-6. Tubing Weight in Reservoir
Hose Fittings
Waste Drain
2. Decide whether you want the waste tubing to extend from the left or
right side of the Dionex AutoTrace 280, and then orient the waste
drain's hose fittings to the appropriate side.
3. Install the waste drain onto the elution station's two ball studs, making
sure the waste drain snaps into place and sits flush on the base plate
(see Figure B-8).
Ball Stud
Base Plate
Figure B-8. Ball Stud and Base Plate
4. Push the waste drain toward the back of the system; it moves only
about 3 mm (1/8 in). This correctly positions the drain under the
manifold.
5. Note the 2.44-m (8-ft) length of tubing (P/N 071147) connected to the
caps of the two 12-liter waste containers (P/N 071159). One piece of
tubing is for aqueous waste; the other is for solvent waste. Screw the
caps onto the waste containers.
6. With one hand supporting the waste drain, press the free ends of the
waste tubing onto the drain's hose fittings (see Figure B-9).
8. Locate the tubing port labeled WASTE directly below the five solvent
tubing lines on the left side of the system (see Figure B-10).
Waste Port
Figure B-10. Waste Tubing Port
9. Insert the waste tubing from this port into one of the bottles supplied
with the system, keeping the following in mind:
• Make sure the end of the tubing extends just below the top of the
container.
• Avoid sharp turns and uphill pieces of tubing from the system to
the container.
Exhaust
Port
Gas Inlet
Figure B-14. Gas Inlet
4. Using the supplied 1/4-in NPT x 1/8-in barb fitting (P/N 071151),
connect the other end of the tubing to the gas supply.
5. Turn on the gas supply. The gas supply must be clean and filtered
with an incoming pressure of no more than 0.69 MPa (100 psi).
6. Before operating the Dionex AutoTrace 280, you must set the system
pressure to 0.07 MPa (10 psi). See Section 3.5 for instructions.
10. Turn on the power to the Dionex AutoTrace 280 and the PC.
1. Locate the USB cable (P/N 960777) provided in the Ship Kit
(standard Dionex AutoTrace 280, P/N 071383; Dionex AutoTrace
280 PFAS, P/N 22136-62005).
The flat, rectangular end of the cable is the “A” connector. The
smaller, hexagonal end of the cable is the “B” connector.
2. Plug the “A” connector of the USB cable into a USB 2.0 port on the
PC on which AutoTrace software is installed.
3. Plug the “B” connector of the USB cable into the USB receptacle on
the right side of the Dionex AutoTrace 280 (see Figure 2-5).
The power supply cord is used as the main disconnect device. Make
sure the socket-outlet is located near the Dionex AutoTrace 280 and is
easily accessible.
Disks
071059 Disk Holder O-Ring Kit Pkg. of 6
Syringes
070579 Liquid handling syringe, 10 mL 1
070296 Air push syringe, 10 mL 1
Sample Pumps
071529 Sample pump for Dionex AutoTrace 280 cartridge 1
model
071530 Sample pump for Dionex AutoTrace 280 disk 1
model
Valves
071329 12-port valve and motor 1
072592 12-port valve rotor 1
072593 12-port valve stator 1
072589 12-Port Valve Fittings Kit (includes 14 nuts and 1
ferrules)
070325 Air push valve 1
070273 Switching valve 1
Miscellaneous
071453 Dionex AutoTrace 280 Preventive Maintenance Kit 1
(for cartridge model)
075900 Dionex AutoTrace 280 Preventive Maintenance Kit 1
(for disk model)
083614 Dionex AutoTrace 280 Sample Cleanup Kit 1
954745 Fuse, 3.15 amp IEC127 fast-blow 1
960777 USB cable, 1.8 m (6 ft) 1
960779 USB cable, 5 m (5.5 yds) 1
Air Factor
The adjustable factor that determines the distance liquid is pushed beyond the 12-
port valve and the switching valves. The air push factor is used in Solvent
Conditioning and Elute to Soak steps to push the liquid partially through the
cartridge or disk path. This prevents the bed of the cartridge or disk from
becoming dried out.
The selected setting (from 0.3 to 5) increases or decreases, respectively, the
standard 1.2 mL of air that follows the liquid being sent through the cartridge by a
factor of 0.3 to 5 times.
If volumes greater than 5 mL are required, the Dry with Gas step in “Creating a
Method to Clean the Sample Lines” on page 45 is recommended.
Air Push
A flow of air generated by the air push syringe that clears liquid from the liquid
handling syringe and tubing lines after conditioning, rinsing, eluting, and syringe
washing steps.
AutoTrace Software
Software used to set up, create, and review a Dionex AutoTrace 280 method and
then run the method to control operation of the Dionex AutoTrace 280.
Flow Rate
The speed (in mL/min) at which to run the Dionex AutoTrace 280 syringes. Flow
rates for solid-phase conditioning, loading, rinsing, and eluting operations are
designated in the Set Up Parameters window in the AutoTrace software.
Method
The list of steps that defines the operating conditions for a run. Methods are
created in the AutoTrace software and used by the software to run the samples.
Push Delay
The duration (in seconds) of the pause after liquid has been sent through the
cartridge or disk. This delay allows the pressure to equilibrate.
C D
Cables Daily maintenance, 64
Power, 143 Daily operation checklist, 64
USB, 143 Danger icon, 4
Calibrate Pump diagnostic method, 71 Diagnostic methods, 12, 70
Cartridge conditioning diagrams Dionex Technical Support, 4, 67, 79
Air Push with Air Factor of 0.5, 24 Disk holder
Air Push with Air Factor of 1.0, 23 Compression fittings, 140
Liquid Handling and Air Push Syringes Dispersing disks, 53, 148
Fill, 19 Removing used disks, 60
Liquid Syringe Dispenses Solvent, 21 Disk holder O-ring
Cartridge conditioning flow charts Leaks, 76
Air Push, 22 Replacing, 85
Liquid Handling and Air Push Syringes Dispersing disks, 53, 148
Fill, 18 Display and keypad specifications, 124
Liquid Syringe Dispenses Solvent, 20 Dry with Gas diagram, 34
Cartridge holder, 9 Dry with Gas flow chart, 33
Compression fittings, 140
Conversion procedure, 96, 99
LED does not light, 76 E
Cartridge holder O-ring
Electrical specifications, 123
Leaks, 52
Elute to Collect diagrams
Replacing, 84
Air Push, 40
Cartridge rinse diagrams
Liquid Handling and Air Push Syringes
Air Push, 32
Fill, 36
Liquid Handling and Air Push Syringes
Liquid Handling Syringe Dispenses
Fill, 28
Solvent, 38
Liquid Handling Syringe Dispenses
Solvent, 30 Elute to Collect flow charts
Cartridge rinse flow charts Air Push, 39
Air Push, 31 Liquid Handling and Air Push Syringes
Liquid Handling and Air Push Syringes Fill, 35
Fill, 27 Liquid Handling Syringe Dispenses
Liquid Handling Syringe Dispenses Solvent, 37
Solvent, 29 Elution containers, 2
Cartridges, 12 Elution manifold
Removing packing debris, 52 Nozzle clogged, 77
Removing used cartridges, 59 Elution nozzles
Caution icon, 4 Aligning, 119
Compression fittings Elution rack, 14
Checking tightness, 139 Elution station, 10, 14
Computer connection, 142 Position is incorrect, 77
Elution volume, maximum
S
N Safety icons, 4 – 5
Safety messages, 4 – 5
Nitrogen manifold fittings
Sample Cleanup Kit, 2
Tightening, 83
Sample input lines, 15
Nitrosamine compounds, 2
Cleaning, 60
NOTES in manual, 5
Connecting to sample containers, 54
Sample pumps, 9
Calibrate Pump diagnostic method, 71
O Calibrating, 103
Operating humidity, 123 Cleaning cylinder and piston, 101
Operating temperature, 123 Compression fittings, 140
Operation Failure to start, 77
Application tips, 47 Replacing, 104
Overview, 43 Sample rack
Shutdown, 66 Installing, 53
See also Methods Service procedures
12-port valve replacement, 109
Air push syringe replacement, 91
P Air push valve replacement, 112
Benchmark Test diagnostic method, 117
Pausing a run, 56
Cartridge holder conversion, 96, 99
PC specifications, 142
Valve leaks, 73
Water coming off with eluent, 77
Wrong liquid volumes delivered, 73
Wrong solvent dispensed, 73
Wrong volume from sample container, 77
Tubing connections
Replacing, 79
Tubing Kits, 80
U
USB cable, 149
Installation, 143
USB port, 3, 15
V
Valve
See 12-port valve
See Air push valve
See Switching valves
Volatile compounds
Recovery is low, 76
W
Warning icon, 4
Waste drain connections
Installation, 133
Waste lines, 14, 135
Waste ports, 13