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    Phagocytosis Block 5

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    Mark Johnuel Duavis
    Phagocytosis is a form of endocytosis where macrophages, neutrophils, and dendritic cells internalize pathogens. [1] These immune cells contain, kill, and process microorganisms for antigen presentation, representing a vital part of the innate immune response. [2] The document describes a procedure where buffy coat from blood is mixed with bacterial cultures, incubated, then smeared and stained to observe phagocytosis under a microscope. [3] Finding only lymphocytes in the smear would signify that phagocytosis did not occur, as these are not phagocytic immune cells.

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    Phagocytosis Block 5

    Uploaded by

    Mark Johnuel Duavis
    88 views25 pages
    Phagocytosis is a form of endocytosis where macrophages, neutrophils, and dendritic cells internalize pathogens. [1] These immune cells contain, kill, and process microorganisms for antigen presentation, representing a vital part of the innate immune response. [2] The document describes a procedure where buffy coat from blood is mixed with bacterial cultures, incubated, then smeared and stained to observe phagocytosis under a microscope. [3] Finding only lymphocytes in the smear would signify that phagocytosis did not occur, as these are not phagocytic immune cells.

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    Phagocytosis Presentation School of Medicine

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    Phagocytosis is a form of endocytosis where macrophages, neutrophils, and dendritic cells internalize pathogens. [1] These immune cells contain, kill, and process microorganisms for antigen presentation, representing a vital part of the innate immune response. [2] The document describes a procedure where buffy coat from blood is mixed with bacterial cultures, incubated, then smeared and stained to observe phagocytosis under a microscope. [3] Finding only lymphocytes in the smear would signify that phagocytosis did not occur, as these are not phagocytic immune cells.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    88 views25 pages

    Phagocytosis Block 5

    Uploaded by

    Mark Johnuel Duavis
    Phagocytosis is a form of endocytosis where macrophages, neutrophils, and dendritic cells internalize pathogens. [1] These immune cells contain, kill, and process microorganisms for antigen presentation, representing a vital part of the innate immune response. [2] The document describes a procedure where buffy coat from blood is mixed with bacterial cultures, incubated, then smeared and stained to observe phagocytosis under a microscope. [3] Finding only lymphocytes in the smear would signify that phagocytosis did not occur, as these are not phagocytic immune cells.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 25

    EXERCISE 5

    PHAGOCYTOSIS
    Block 5
    Batch Tritonos
    What is phagocytosis?
    • Phagocytosis:
    - a specific form of endocytosis by which
    cells internalize solid matter, including
    microbial pathogens.
    - Macrophages, Neutrophils and Dendritic
    cells
    - In these cells, phagocytosis is a
    mechanism by which microorganisms can
    be contained, killed and processed for
    antigen presentation and represents a vital
    facet of the innate immune response to
    pathogens, and plays an essential role in
    initiating the adaptive immune response.
    Materials:

    Freshly drawn blood for buffy coat


    sample using heparinized tube

    Buffy
    coat
    Materials:
    Broth cultures of Staphylococcus
    aureus and Escherichia coli

    Staphylococcus aureus

    Escherichia coli
    Materials:
    c. Pasteur pipettes d. Wright stain set-up
    Procedure:
    1. Transfer 5 ml blood into an evacuated tube with heparin. Separate
    plasma by centrifugation and discard.

    2. With a Pasteur pipette extract the buffy coat

    Buffy
    coat
    Procedure:
    3. Place 4 drops of buffy coat into an empty tube.

    4. Add 2 drops of assigned broth culture to the tube with buffy coat.
    Blocks 1,3,5,7: Staphylococcus aureus broth
    Blocks 2,4,6,8: Escherichia coli broth

    5. Incubate the inoculated tubes at 37 degrees Celsius for 30 minutes.


    Procedure:
    6. Place 1 drop of the incubated specimen onto a slide and make a smear.

    7. Air dry the smear.


    Procedure:
    8. Stain the prepared smear with Wright’s stain in the following manner
    a. Place the slide in a horizontal position on a rack and add Wright’s
    stain covering the surface of the smear completely. Allow the stain to
    stand for 1-3 minutes
    b. Add an equal amount of drops of buffered water to bring the
    meniscus to the margin. Leave the mixture for 8-10 minutes. The
    presence of a green metallic sheen is indicative of proper staining.
    c. Wash the stain off with running water. Do not pour off the stain before washing
    because this will leave a precipitate on the smear.

    d. Wipe the bottom of the slide to remove the dried stain.


    DO NOT BLOT DRY
    Results:
    • Block 1
    Results:
    Block 2
    Results:
    Block 4
    Results:
    Block 5
    Results:
    Block 6
    Results:
    Block 7
    Results:
    Block 8
    Phagocytic cells

    macrophage neutrophil dendritic cell


    Steps in phagocytosis:
    CHEMOATTRACTANTS
    • Bacterial products
    • Activated complement factors (c3a,c5a)
    Step 1: Activation of the Phagocyte • Arachidonic acid metabolites (LTB4)
    • Cytokines: such as IL-1, IL-8
    Step 2: Chemotaxis of Phagocytes
    (for wandering macrophages, neutrophils, and eosinophils)
    Step 3: Attachment of the Phagocyte to the Microbe or Cell
    Step 4: Ingestion of the Microbe or Cell by the Phagocyte
    Step 5: Destruction of the microbe or cell
    Phagocytosis
    Question

    Give the significance of finding only


    lymphocytes in the smear.


    Thank you!

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