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How Wavelength of Light Affects Photosynthesis.: Control Group

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How wavelength of Light affects photosynthesis.

The null hypothesis is that Wavelength of light that is green light decrease photosynthesis.

The alternates hypothesis states increasing wavelength of light such as red or violet will have a
significant positive impact on the potential growth of the plants as they are green they are
absorbing the other wavelengths of light but reflecting back the green. This would allow them to
go through many steps of Light dependent and light Independent reaction, producing greater
amount of oxygen and glucose.

The Independent variable


The wavelength of the light is being varied by altering the light color ( 5 different colors)
including white color.

Dependent variable
The rate at which the plant Elodea synthesises

Control variable
Light intensity: the ​lamp will be kept 10cm away from the Elodea plant at all times hence no
additional effect on the rate of photosynthesis.

Room temperature in degrees Celsius: the temperature of the room will be kept 25 degrees celsius
at all times as well as no air conditioning or open windows will be present to prevent any additional
affect on the rate of photosynthesis.

Concentration of Sodium hydrogen Carbonate: the concentration will be 5 mol/dm3 at all times to
prevent any additional affect on the rate of photosynthesis.

Time provided for measuring the rate of photosynthesis: the bubbles produced will be measured
every 40 seconds.

Control group: ​Another Elodea plant is given normal conditions and they are not provided with
additional features that could increase or decrease their rate of photosynthesis. Their wavelength of
light should be red at all times. This allows us to compare the rate at how both the Elodea plants
differ in terms of photosynthesis.

Apparatus:
-Light bulb
-Elodea plant
- 1% of 200cm3 of Sodium hydrogen carbonate
-200 cm3 beaker
-thermometers
-safety goggles
-5 colour filters
-boiling tube
-stop watch
-ruler

Method
1- set up the apparatus
2- add your sample of elodea plant into the boiling tube
3-Fill boiling tube containing the elodea plant with 1% Sodium Hydrogen Carbonate. Fill it till it
covers the plant.
4-record the temperature (make sure it is at 25 degrees celsius)
5-Plug in the 100W light bulb in and turn it on to its side so that it is facing the beaker. Place it 10cm
away from the beaker.
6-Allow the beaker to adapt for 2 minutes and return to starting temperature before beginning net
experiment
7-After 2 minutes count the number of bubbles that appeared with 40 second increments in 2 ½
minutes.
8-Record the number of bubbles given off
9-Change the colour of the filter and allow the plant to acclimatise for 2 minutes again before
repeating the experiment.
10-Repeat the experiment again with a different wavelength of light
11- put your results in a table and draw the graph: showing how rate alters with wavelength of
light.

Risk assessment

Risk Why it is harmful Solution

Bright Light It could cause damage to the The student is not facing the
retina of the eye light directly

Sodium hydrogen carbonate Could cause damage to the Wear safety goggles
eyes if comes in contact with
it

Beaker Made of glass and can break It is kept away from the edge
of the floor

By: Suraksha Mukesh

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