Boza-Oviedo 2012 Zootaxa
Boza-Oviedo 2012 Zootaxa
Boza-Oviedo 2012 Zootaxa
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Abstract
We describe five new species of lungless salamanders (Plethodontidae) from high mountain habitats along the border between
Costa Rica and Panama: Bolitoglossa splendida, Bolitoglossa aureogularis, Bolitoglossa kamuk, Nototriton matama, and
Oedipina nimaso. We also present phylogenetic hypotheses for the new taxa (with the exception of the Oedipina) and their rel-
atives based on mitochondrial DNA sequence data. The new species were discovered on a series of expeditions to remote areas
(primarily over 1000 m elevation) of the Cordillera de Talamanca, Costa Rica.
Key words: Bolitoglossa aureogularis sp. nov., B. kamuk sp. nov., B. splendida sp. nov., Nototriton matama sp. nov.,
Oedipina nimaso sp. nov., biogeography, Central America, Cordillera de Talamanca, taxonomy
Resumen
Se describen cinco nuevas especies de salamandras sin pulmones (Plethodontidae) de hábitats montanos altos de la región fron-
teriza entre Costa Rica y Panamá: Bolitoglossa splendida, B. aureogularis, B. kamuk, Nototriton matama, y Oedipina nimaso.
Además, se presentan hipótesis filogenéticas basadas en secuencias de ADN mitocondrial para los nuevos taxones con excep-
ción de Oedipina. Las nuevas especies fueron descubiertas durante una serie de expediciones a regiones remotas (principal-
mente sobre los 1000 m de elevación) de la Cordillera de Talamanca, Costa Rica.
Introduction
Despite its relatively small land area, Costa Rica has a highly diverse amphibian fauna, of which 44 species are
salamanders of the family Plethodontidae (www.AmphibiaWeb.org). Among tropical countries only Mexico and
Guatemala, both considerably larger, have more known species of plethodontid salamanders, and Costa Rica has
more described salamander species for its size than any other country (Bolaños & Wake 2009). While the
amphibian fauna of much of the country is well known compared with other countries in Central America,
particularly at long-term research sites such as Monteverde and La Selva, other areas of the country have been
much less thoroughly explored biologically. The rugged Cordillera de Talamanca of southeastern Costa Rica
(southeast of the Chirripó Massif) and western Panama is one of the most poorly explored montane areas of Central
America; the portion within the transnational Parque La Amistad is roadless and access is difficult, which has
hindered biological exploration in the past. The Cordillera de Talamanca consists of uplifted Neogene-Quaternary
rock and reaches elevations up to 3 820 m (Marshall 2007). Collections from other areas of the border region
Itinerary of expeditions
The first of all our expeditions was on April 17-20, 1984 and was carried out by Douglas C. Robinson, Gilbert
Barrantes and F. Bolaños. The trip was part of a larger trip to the Caribbean of Costa Rica and the Talamancan part
of the trip started in Suretka (Talamanca, Limón, 9.5785° N, 82.9445° W, 50 m elevation; all GPS coordinates in
WGS84 datum), where the team arrived on April 17 (Fig. 1). The next day they hiked past Amubri to Río Lari,
arriving at the northeast slope of Cerro Nimaso (9.4833° N, 82.0278° W, 700 m elevation), where they spent two
nights. On April 20, they went back to Suretka and moved to another site out of the Talamancan mountain range. A
two-day effort was made at the second site and many new records for the country were collected there. The two
salamanders collected, both on the NE slope of Cerro Nimaso, were Oedipina nimaso sp. nov. and the first Costa
Rican report of Bolitoglossa schizodactyla. All areas sampled were within a reserve for indigenous people, which,
while forested, showed clear signs of use.
The Trans-Talamancan expedition took place between February 18 and March 10, 2007. The trip was
organized by botanists of the Instituto Nacional de Biodiversidad (INBio), with the participation of E. Boza-
Oviedo, whose role was to evaluate amphibians and reptiles. The trip began in the town of Ujarrás (Buenos Aires,
Puntarenas, 9.2331° N, 83.3066° W, 500 m elevation) in the Pacific versant of the Talamanca mountain range (Fig.
1). After two days of 6 hr hikes, the team reached the first camp, located near the headwaters of Río Lori (9.3580°
N, 83.2295° W, 1817 m elevation), a tributary of Río Coén near the continental divide on the Caribbean versant.
The team sampled for nine days in an area of cloud forest vegetation. In the vicinity of the camp, a lone specimen
of Bolitoglossa splendida sp. nov. was found. In a cloud forest at 2100 m elevation (9.3416° N, 83.2320° W), 2 hr
from camp on foot, the first three specimens of B. aureogularis sp. nov. were found. A one-day hike led the team to
the second camp (9.3925° N, 83.2119° W), located at 1700 m on the bank of the upper stretches of the Río Coén,
where they remained for 6 days; this area is also cloud forest and near the continental divide. Here two additional
specimens of B. aureogularis sp. nov. were found in the vicinity of the camp. The team then began the return trip,
spending two days in a third camp and another day in a fourth camp; at the fourth camp (9.4437° N, 83.2040° W),
B. robusta was captured while trapping beetles at 1000 m elevation. They then walked to the Río Coén and on to
Sepeque (9.5611° N, 83.9909° W, 100 m elevation), where they traveled by boat to Suretka. Areas searched on the
trip were between 400 m and 2300 m elevation.
The third expedition, to Laguna Dabagri (9.6183° N, 83.2833° W, 1000 m elevation), lasted from July 17–30,
2007. The trip started in Alto Blei (9.6175° N, 83.2489 ° W, 800 m elevation), an indigenous community near La
Amistad International Park in the Caribbean part of the Talamanca Mountain Range (Fig. 1), where the group
arrived by helicopter. The trip covered an altitudinal range of 800-1500 m. The lake is located at 1000 m (9.6183°
N, 83.2833° W) in Fila Matama (near Fila Lleskila) where most sampling effort was made. Little description of this
trip is given because no salamanders were found.
The fourth expedition, to Fila Matama, lasted from October 21 to November 3, 2007. The trip started at the
town of Aguas Zarcas (Limón, Limón), also organized by botanists but with the participation of E. Boza-Oviedo
and Ruth Delgado to evaluate amphibians and reptiles. From the village, they traveled on the Río Aguas Zarcas, a
tributary of Río Banano (Fig. 1). The first camp was established en route to Fila Matama on a hill north of the ridge
at 750 m elevation (9.8242° N, 83.1617° W), where only one night of sampling took place. The next day, the group
moved to the second camp at 1300 m elevation near the top of the ridge (Fila Matama, 9.8132° N, 83.1681°W),
where they sampled for nine days in wet forest. Specimens of Nototriton matama sp. nov. were found near the
camp in moss mats. The team spent two more days in camp 1 on the return trip to Aguas Zarcas. The only other
salamander found on this trip was Bolitoglossa colonnea, one specimen from ca 750 m (9.8242° N, 83.1617° W) m
and another at 1200 m elevation (9.8174° N, 83.1667° W). Areas searched on this trip were between 200 m and
1400 m elevation.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 37
FIGURE 1. Map of the border region between Costa Rica and Panama, showing expedition routes and type localities for new
salamander species.
Fieldwork and sampling methods: Salamanders were sought by day in moss mats, under logs, by raking through
leaf litter, and by opening bromeliads both in trees and on the ground. By night, salamanders were sought using
lights. Identification of plant species as part of habitat descriptions was with the help of botanists, who studied
fresh material collected during these surveys. Temperature measurements were taken using a standard laboratory
alcohol thermometer. All salamanders were fixed in 10% formalin and transferred to ethanol for long-term storage.
Tissue samples (liver or tail tip) were taken from salamanders from all but the 1984 trip to Cerro Nimaso, and tis-
sues were preserved in DMSO buffer or in 95% ethanol.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 39
Morphology: The following measurements were used for morphological comparison: snout to posterior angle
of vent (standard length, SL), head width (HW), snout to gular fold (SG), head depth at posterior angle of jaw
(HD), eyelid length (EL), eyelid width (EW), anterior rim of orbit to tip of snout (ES), horizontal eye diameter
(ED), interorbital distance between angle of eyes (intercanthal distance, IC), interorbital distance between eyelids
(IO), snout to forelimb (SF), distance separating external nares (internarial distance, IN), snout projection beyond
mandible (SP), shoulder width (SW), snout to anterior angle of vent (SAV), axilla-groin distance (AX), number of
costal interspaces between adpressed limbs (limb interval, LI), forelimb length (FLL), hind limb length (HLL),
hand width (HAW), foot width (FW), length of third (longest) toe (T3), length of fifth toe (T5). Measurements
were made using digital or dial calipers or a dissecting microscope fitted with an ocular micrometer; standard
length (SL) was measured from the anterior tip of the snout to the posterior angle of the vent. Limb interval equals
the number of costal interspaces between the tips of adpressed fore- and hind limbs, measured in one-half
increments (e.g., 3, 3.5). Radiographs were prepared for the holotype of the newly described Oedipina. Counts of
presacral (trunk) vertebrae do not include the first, or atlas, vertebra. Tooth counts are based on direct counts of
clearly ankylosed teeth. Numbers of maxillary (MT) and vomerine (VT) teeth in each holotype are provided for
right and left sides, along with number of premaxillary teeth (PMT); these counts are summed for other individuals.
Institutional abbreviations are as listed in Leviton et al. (1985). Color information was derived from photographs of
living specimens.
Phylogenetic analysis: DNA was extracted from liver tissue or tail tips using a Qiagen DNEasy tissue kit
(Qiagen, Valencia, California). We sequenced DNA from field-collected samples of Bolitoglossa and Nototriton
for the 16S rRNA (16S) and cytochrome b (cyt b) mitochondrial genes. Primers MVZ117 and MVZ98 (Palumbi et
al. 1991) were used for 16S and primers MVZ15 and MVZ16 (Moritz et al. 1992) for cyt b. Reactions were run at
94 ºC for 2 min, 38 cycles of 94 ºC for 30 s, 48 ºC for 30 s (16S) or 1 min (cyt b), 72 ºC for 1 min, with a final cycle
at 72 ºC for 8 min. A list of samples used for DNA sequencing and phylogenetic analysis, along with GenBank
numbers, is given in Table 1. We aligned these sequences with available sequences for Talamancan salamanders
(Bolitoglossa) or other members of the genus (Nototriton) from GenBank using the program MUSCLE 3.6 (Edgar
2004) and concatenated alignments for 16S and cyt b. Sequences of cyt b for Nototriton were trimmed to match the
shorter fragment length of available sequences from GenBank. The aligned 16S sequences had a length of 529 bp
(Bolitoglossa) and 517 bp (Nototriton), while the cyt b alignment had a length of 809 bp (Bolitoglossa) and 385 bp
(Nototriton). We used the program RAxML (Stamatakis 2006) to estimate a phylogeny with maximum likelihood
under the General Time Reversible substitution model (Tavaré 1986) with invariable sites and among-site rate
variation (GTR+I+G) substitution model with 4 data partitions (corresponding to 16S and codon positions 1, 2 and
3 of cyt b) for both Bolitoglossa and Nototriton, and 1000 bootstrap replicates were performed to assess nodal
support. We also estimated the phylogeny using a partitioned Bayesian phylogenetic analysis implemented in
MrBayes 3.1.2 (Huelsenbeck & Ronquist 2001). As for the ML analysis, the data were partitioned by gene (16S
and cyt b), and the cytochrome b gene was partitioned by codon position. We used MrModeltest 2.2 (Nylander
2004) to select a substitution model for each partition. The following substitution models were used for each
partition: GTR+I+G for Bolitoglossa 16S, Bolitoglossa cyt b codon position 3, and Nototriton 16S, Kimura (1980)
model with a rate heterogeneity parameter (K80+G) for Bolitoglossa cyt b codon position 1, K80+I+G for
Nototriton cyt b codon position 1, Hasegawa-Kishino-Yano (Hasegawa et al. 1985) model with a rate heterogeneity
parameter (HKY+G) for Bolitoglossa cyt b codon position 2, HKY for Nototriton cyt b codon position 2, and
GTR+G for Nototriton cyt b codon position 3. The analysis was run for 20,000,000 generations, and 5,000,000
generations were discarded as burn-in. Bolitoglossa mexicana was used as an outgroup for all phylogenetic
analyses for Bolitoglossa, and Oedipina alleni was used as an outgroup for all phylogenetic analysis for Nototriton.
Convergence of split frequencies was assessed using the compare and sliding window plots in program AWTY
(Nylander et al. 2008). Average pairwise Tamura-Nei (TN) (Tamura & Nei 1993) distances between taxa for both
16S and cytb were calculated using Arlequin v3.5 (Excoffier et al. 2005).
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 41
TABLE 1. (Continued)
Voucher Number Species GenBank 16S GenBank cyt b
MVZ 231322 Bolitoglossa pesrubra — AF212074
DBW 5117 Bolitoglossa pesrubra — AF212081
DBW 5118 Bolitoglossa pesrubra — AF212082
Cerro Asunción Bolitoglossa pesrubra — AF212080
Cerro Sakira Bolitoglossa pesrubra — AF212079
23.5 km SE El Empalme Bolitoglossa pesrubra — AF212078
La Georgina Bolitoglossa pesrubra — AF212083
Ojo de Agua Bolitoglossa pesrubra — AF212077
Salsipuedes Bolitoglossa pesrubra — AF212070
Villa Mills Bolitoglossa pesrubra — AF212084
UCR 20489 Bolitoglossa robinsoni JQ899161 JQ899191
UCR 19835 Bolitoglossa splendida JQ899150 JQ899181
CH 7478 Bolitoglossa sombra JQ899165 JQ899168
UCR 225871 Bolitoglossa sombra AY526136 AY526174
MVZ 194828 Bolitoglossa subpalmata AF212091 EU448107
UCR 12065 Bolitoglossa tica AY526137 AF212089
UCR 20514 Bolitoglossa tica JQ899162 JQ899192
UCR 12071 Nototriton abscondens AF199130 AF199130
JHT 2420 Nototriton barbouri GU971734 GU971734
UTA A-51490 Nototriton brodiei AF199139 AF199139
MVZ 207122 Nototriton gamezi AF199135 AF199135
MVZ 207106 Nototriton guanacaste AF199140 AF199140
USNM 497540 Nototriton lignicola AF199141 AF199141
MVZ 263852 Nototriton limnospectator JN377383 JQ899197
UCR 20215 Nototriton matama JQ899166 JQ899195
MVZ 225899 Nototriton picadoi AF199144 AF199144
UCR 12057 Nototriton richardi AF199146 AF199146
MVZ 230241 Nototriton saslaya GU981761 —
USNM 339712 Nototriton sp. AF199136 AF199136
USAC 3357 Nototriton stuarti JQ899167 JQ899196
UF 155377 Nototriton tomamorum GU971731 GU971732
MVZ 190857 Oedipina alleni AF199207 AF199149
Holotype. UCR 19835, an adult female from the headwaters region of the Lori branch of the Río Coén near Cerro
Arbolado (coordinates 9.3567º N, 83.229º W) at 1826 m elevation, Provincia de Limón, Costa Rica, collected 19
February, 2007 by Marco Moraga and Eduardo Boza-Oviedo.
TABLE 2. Measurements for specimens used in description of new species, as well as other specimens collected on Talaman-
can expeditions used for comparison to new taxa.
Coloration of the holotype in life (Fig. 2). Specimen is glossy dark black, somewhat paler ventrally than
dorsally and laterally. A broad bright red to orange-red band arises on back of head and extends to posterior end of
body. Band is bifurcated anteriorly into two stripes that arise from posterior margin of eyelids. Medial borders of
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 43
these stripes converge, meeting at about level of nuchal fold, behind angle of the jaw and about 3 mm anterior to
level of gular fold. Band bright and uninterrupted although there is some fine speckling of melanin in area anterior
to shoulders. Lateral border of band irregular, encroaching onto the dorsal surface immediately in front of
shoulders and then descending onto upper flanks immediately behind shoulders. Black pigment present between
band and insertion of both forelimbs and hind limbs. Bright yellow spots with glossy enamel-like finish present
laterally and ventrolaterally. On right side there is one spot in front of limbs, six spots on side of trunk, and one
behind limbs; on left side there are five spots on side of trunk and one on posterior insertion of hind limb. Venter
immaculate.
FIGURE 2. A) Dorsal and B) ventral views of the holotype (UCR 19835) of Bolitoglossa splendida in life. C) Dorsal view of
left hand and D) left foot of holotype of B. splendida. Photos A and B by Alex Monro and E. Boza-Oviedo, C and D by A.
García-Rodríguez. The bar in hand and feet are 1 mm length.
Habitat and range. The species is known only from the type locality, which lies a short distance north of the
continental divide along a well-known trail that crosses from the Pacific side to the Caribbean side of the main Cor-
dillera de Talamanca in eastern Costa Rica. The type locality lies along the Río Lori, a highland tributary of the Río
Coén, which flows north and east toward the Caribbean. The crest of the Cordillera de Talamanca is relatively low
in this area, and the type locality is below Cerro Arbolado (2500 m elevation). The specimen was active on the
upper side of a Heliconia sp. leaf at 19:50 h, 1.1 m above ground. It was about 100 m from the river and 30 m from
a smaller stream. Air temperature was 13 °C on a day that had a heavy rain at 11:00-14:00, and the vegetation and
soil were moist. The vegetation at this locality is mature cloud forest, consisting primarily of members of the
Araceae (e.g. Philodendron, Anthurium, Monstera), Heliconiaceae (Heliconia), Marantaceae, Begoniaceae, Acan-
thaceae, Arecaceae (Geonoma), Commelinaceae, Rubiaceae, Urticaceae, Melastomataceae, Bromeliaceae, Eri-
caceae, Piperaceae, bryophytes and ferns.
Etymology. This species name is derived from splendidus (L.), a word that denotes the exceptionally brilliant
coloration of this species.
Holotype. UCR 19893, an adult female from along the Río Coén on the Trans-Talamancan trail near Cerro Arbo-
lado (9.3925º N, 83.2119º W) at an elevation of 1680 m approximately 8 km N of the continental divide, Provincia
de Limón, Costa Rica, collected by Eduardo Boza-Oviedo on 1 March 2007.
Paratypes. UCR 19892, same data as holotype; UCR 19857–59 (3 specimens), 9.3416º N, 83.232º W, 2102
m, in headwaters area of Río Coén, about 2 km N continental divide, Provincia de Limón, Costa Rica, collected by
Eduardo Boza-Oviedo on 22 February 2007.
Diagnosis. Assigned to Bolitoglossa because it lacks a sublingual fold (Wake & Elias 1983), and to subgenus
Eladinea based on mtDNA sequence data. A medium-sized member of the genus Bolitoglossa (subgenus Eladinea)
with moderate webbing of the digits of the hands and feet that differs from all other species in the genus by its
unique coloration (Fig. 3) of reddish tan to yellow dorsal coloration with black flanks and a venter marked by
bright yellow gular and yellow-brown chest regions, with a pair of dirty white patches on the ventrolateral surfaces
of the posterior venter. In comparison to members of the B. robinsoni complex, this species is much smaller and
more slender, in addition to the coloration differences.
FIGURE 3. A) Dorsal and B) ventral views of holotype (UCR19893) of Bolitoglossa aureogularis in life. C) Paratype
(UCR19857) of B. aureogularis in life. D) Dorsal view of right hand and E) right foot of holotype. Photos A, B and C by E.
Boza-Oviedo and Roney Samaniego, D and E by Adrián García-Rodríguez. The bar in hand and feet are 1 mm length.
Description. A slender species of moderate size compared to other members of its genus. SL of holotype, the
only adult specimen available (48.8 mm), nearly identical to that of its close geographic neighbor Bolitoglossa
splendida and close to mean value of such Talamancan species as B. pesrubra, B. subpalmata (García-París et al.
2008), and B. gomezi (Wake et al. 2007). Tail slender but relatively short (tip broken). Head narrow (Wake &
Brame 1972); SL/HW = 8.0. Relatively short snout broadly rounded. Small nostrils are typical for this genus.
Nasolabial protuberances not pronounced; paler than surroundings and appear to be pigmented with white. Eyes
small, do not protrude beyond lateral margins of head, not visible in ventral view. Teeth moderate in size and
numerous (57 MT, 6 PMT, 27 VT). Limbs relatively short with LI of 3.5. Hands and feet moderate in size; FW =
5.1 mm. Digits well-differentiated but short and knob-like with distinct subdigital pads on longer digits. Webbing,
reaching between first and second phalangeal articulations of longest digits; webbing more extensive in foot than in
hand. Fingers, in order of decreasing length, are 3-4-2-1; toes are 3-4-2-5-1. Postiliac glands pale and
inconspicuous.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 45
Measurements (in mm), limb interval and tooth counts of the female holotype (Table 2). HW 6.1, SG
10.1, HD 2.3, EW 1.3, EL 2.7, ES 1.7, ED 2.2, IC 3.5, IO 2.4, SF 12.5, IN 1.5, SP 0.4, SW 4.8, SL 48.8, SAV 45.0,
AX 26.2, LI 6.5, FLL 9.3, HLL 10.2, HAW 3.3, FW 4.3, T5 0.9, T3 1.4. Numbers of teeth: PMT 6, MT 29/28, VT
13/14.
Coloration of the holotype in life (Fig. 3). Dorsal coloration golden-tan with some bright highlights on dorso-
lateral regions and with some narrow streaks of dark brown. Golden-tan coloration in form of broad band extending
from snout onto tail. Tail becomes increasingly reddish brown posteriorly. Lateral surfaces dark brown with numer-
ous white speckles. Dark coloration forms lateral margin of dorsal band and continues onto tail and forward all the
way to eye and is present almost to tip of snout. Dark coloration extends to area above limb insertions so light band
does not contact limbs. Dorsal surfaces of limbs similar to dorsal band in color. White pigment present ventrolater-
ally along trunk and becomes prominent on venter, where pair of lightly colored patches is separated by region of
dark pigmentation. Gular area bright yellow, which becomes golden on chest before fading into darker color in
midtrunk region. Venter of tail speckled with white and tan spots. Iris golden.
Habitat and range. The species is known from two nearby localities on the Caribbean slope of the Cordillera
de Talamanca, both with mature cloud forest similar to the habitat at the type locality of Bolitoglossa splendida.
The first locality is at ca. 2100 m elevation, 700 m from the nearest stream; the second, the type locality, is located
at 1680 m elevation near the river edge.
Variation. There are four paratypes but only one approaches maturity in size. All specimens show coloration
typical of the holotype, but some are more reddish gold dorsally and they are covered with tiny white speckles.
Even the smallest individual (UCR 19858, 9.2 mm SL) displays the characteristic ventral coloration. This is a slen-
der species with a small, narrow head and slender trunk and tail.
Etymology. The species name is derived from aurea (L.), golden, and gula (L.), throat, in recognition of the
unusual bright yellow coloration of the gular and chest regions of this species.
Natural history and behavioral observations. The specimens from the first site were inactive in bromeliads
(2.8–6.2 m above ground) during daytime, one in a cavity formed by the bromeliad roots in the trunk of the tree and
the other two inside the leaves. All were in different plants in two trees, with two found in separate branches of the
same tree; the temperature of the retreats was 12 °C with an air temperature of 15 °C. The juvenile and holotype
from the second locality were found active at night on low vegetation or in the leaf litter (0–1.45 m), 1.8–3.7 m
from the river with an air temperature of 15–18 °C during the observation period. Behavioral observations were
made from 20:00–00:03 before collecting both specimens. The juvenile moved within the Araceae leaf where it
was found but never went away. The adult female (holotype) climbed to the top of two shrubs and one palm seed-
ling and moved through the leaf litter between plants. On two occasions, it held up a third of its body off surface of
the leaf, and sometimes used its tail as a prehensile organ when moving along steams.
Holotype. UCR 20852, a young adult male from the sub-páramo region atop Cerro Apri in the Kamuk Massif
(coordinates 9.2546º N, 83.059º W) at an elevation of 3126 m, on the continental divide, Provincia de Limón,
Costa Rica, collected 18 December, 2007, by Guillermo Artavia, Gerardo Chaves, Sean Rovito, Guido Saborío and
Hugo Solano.
Paratypes. UCR 20853, 20854; same data as holotype.
Diagnosis. Assigned to Bolitoglossa because it lacks a sublingual fold (Wake & Elias 1983), and to subgenus
Eladinea based on mtDNA sequence data. A slender member of the subpalmata group of Bolitoglossa (Eladinea)
distinguished from all other members of the group by mitochondrial DNA sequences; further distinguished from B.
pesrubra by absence of reddish proximal limb segments and absence of dorsal spots and blotches; from B. gracilis
by more robust habitus and absence of yellowish coloration; from B. tica by smaller hands and feet.
Description. Known only from juveniles and small adults. Appears to be relatively small, slender compared to
other members of genus. SL in two small adult males is 34.8 mm and 33.0 mm. Tails slender, about same length as
SL; SL/TL in two males is 0.99 and 0.98. Head narrow; SL/HW is 6.0 and 6.2. Head well demarcated from neck.
FIGURE 4. A) Dorsal and B) ventral photos of holotype (UCR 20852) of Bolitoglossa kamuk in life. C) Juvenile paratype
(UCR 20854) of B. kamuk in life, showing variation in coloration. D) Dorsal view left hand and E) right foot of preserved holo-
type. Photos A, B and C by S. Rovito, D and E by Adrián García-Rodríguez. The bar in hand and feet are 1 mm length.
Measurements (in mm), limb interval and tooth counts of the male holotype (Table 2). HW 5.8, SG 8.5,
HD 3.1, EW 1.0, EL 2.5, ES 1.7, ED 1.6, IC 2.6, IO 2.6, length of groove extending posteriorly from eye 2.3, dis-
tance between nuchal groove and gular fold 2.6, SF 10.8, IN 1.1, SP 0.4, SL 34.8, SAV 32.0, AX 19.2, LI 1.5, TL
35.2, tail width at base 2.4, tail depth at base 3.0, FLL 9.0, HLL 9.3, HAW 2.5, FW 3.7, T5 0.7, T3 1.1, mental
gland width 1.3, mental gland length 1.2. Numbers of teeth: PMT 2, MT 17/17, VT 8/8, arranged in a single row.
Coloration of the holotype in life (Fig. 4). Dorsum and dorsal surface of tail and hind limbs black with
numerous, uniformly distributed gold flecks. This coloration extends to lateral midline, where gold flecks become
much less numerous. Background of fore limbs somewhat lighter. Gold flecks less numerous on top of head. Venter
is dark grey, with a few gold specks, particularly towards sides of body. Gular region, underside of limbs, and ante-
rior portion of tail pale grey with a few golden specks, while posterior half of the tail darker grey.
Coloration of the holotype in alcohol. Region of head between snout and eyes brown but darkened due to
presence of skin glands that are heavily pigmented peripherally; frontal and parietal areas brown with skin glands
with lighter edges; orbits completely black, canthus rostralis strongly marked numerous black spots on dark brown
background; ventral area cream with numerous black spots that are even more concentrated in anterior region,
making gular region lighter. Dorsum of trunk dark brown that darkens posteriorly; flanks marked with numerous
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 47
black spots on background passing from brown to clear cream from dorsum to venter; venter cream with numerous
dots but still noticeably lighter than dorsum. Tail dark; dorsal part of first three post-sacral segments vertebrae and
underside of first 11 caudal segments maintains color of trunk, while progressively darkening posteriorly, and
becoming completely black. Both hind limbs and forelimbs appear black due to presence of numerous black spots
on dark brown background; dorsal part of hands and ventral part of hands and feet lighter than rest of limb.
Variation. The juvenile paratype (UCR 20854) is orange in dorsal coloration with both dark grey and lighter
colored specks. A darker grey patch is present on the top of the head posterior to the eyes and on the orbits. The
dorsal surface of the limbs and tail are bright orange, changing to a darker orange-grey at the tip of the tail. The
gular region is a pale yellow-orange color, while the venter is a pale golden color with numerous black specks. The
underside of the tail is a uniform orange.
Habitat and range. This species is known only from the type locality on Cerro Apri, southwest of Cerro
Kamuk just off the continental divide. Habitat consists of sub-páramo vegetation with extensive, deep moss mats,
spongy soil, ferns, and small, isolated trees with arboreal bromeliads. The species was found both within these bro-
meliads and under moss.
Etymology. The species is from the Kamuk Massif, named for one of the dominant peaks in the region, Cerro
Kamuk. The scientific name is a noun in apposition.
Holotype. UCR 20215, an apparently mature female from the southeastern end of the Fila Matama (coordinates:
9.8071º N, 83.1683º W) at an elevation of 1300 m, collected on 30 October 2007, by Eduardo Boza-Oviedo and
Ruth Delgado.
Paratypes. UCR20168, 20169, 20171, same data as holotype.
Diagnosis. Assigned to Nototriton because it has a well-developed sublingual fold and has fewer than 17 ver-
tebrae in the trunk, and to the picadoi group based on mtDNA sequence data and on the basis of having rounded
digital tips rather than pointed ones (as in the richardi group). A small member of the Nototriton picadoi group dis-
tinguished from all other members of the group by relatively enlarged and elongated nostrils and small, very nar-
row hands and feet and narrow heads; distinguished from N. picadoi by smaller size (holotype 23.6 mm SL, N.
picadoi reaches 32 mm SL), relatively larger nostril (0.02 times SL, vs. 0.010–0.016 in N. picadoi), and very nar-
row feet (0.05 times SL, vs. 0.06–0.07 in N. picadoi) with pointed outer toe tips (vs. rounded toe tips); distin-
guished from members of the N. richardi group by discrete columnar digits not fused together and by rounded
rather than pointed tips of the longest digits.
Description. A diminutive, slender species compared to other members of its genus. Sole adult specimen
(based on size and proportions), the holotype, has a SL of 23.6 mm. Because the holotype is a female, typically the
larger sex, maximum size of this species is expected to be not much larger than this specimen. Head small and nar-
row (SG/SL= 0.17; HW/SL= 0.14) with broadly truncated snout. Nostrils enlarged and elongated, with maximal
dimension of 0.5 mm. One can see into nostrils from a dorsal view; nostrils oriented mainly frontally. Eyes rela-
tively large and protuberant, visible protruding from side of head when viewed ventrally. Head only moderately
differentiated from trunk, mainly by somewhat enlarged parotoid glands located in temporal region of head. Paro-
toid glands clearly evident as swollen, lightly pigmented oval structures. Teeth moderately abundant; PMT 4, MT
31, VT 11 in holotype. Trunk slender, limbs relatively short (0.18 SL, LI 5). Slender tail only slightly tapered
before a break. Hands and feet bear well-formed, columnar digits that are only slightly webbed basally. Longer dig-
its terminate in rounded tips, but shorter digits have bluntly pointed tips. Fingers, in order of decreasing length, are
3-2-4-1; toes are 3-4-2-5-1.
Measurements (in mm), limb interval and tooth counts of the female holotype (Table 2). HW 3.2, SG 4.2,
HD 1.8, EW 0.6, EL 1.3, ES 0.8, ED 1.0, IC 1.8, IO 0.9, length of groove extending posteriorly from eye 1.0, dis-
tance between nuchal groove and gular fold 0.9, SF 6.8, IN 0.4, SP 0.2, SL 23.6, SAV 21.8, AX 13.4, LI 5, tail bro-
ken at 11.0, tail width at base 1.6, tail depth at base 1.8, FLL 3.7, HLL 4.2, HAW 1.0, FW 1.2, T5 0.2, T3 0.6,
parotoid width 0.5, parotoid length 1.3, nostril diameter 0.5. Number of teeth: PMT 4, MT 15/16, VT 5/6.
FIGURE 5. A) Dorsal view of holotype (UCR 20215) and B) ventral photo of the paratype (UCR 20169) of Nototriton
matama in life. C) Dorsal view of right hand and D) right foot of holotype. Photo A by E. Boza-Oviedo, B by Alex Monro and
E. Boza-Oviedo, C and D by A. García-Rodríguez. The bar in hand and feet are 1 mm length.
Habitat and range. The species is known only from the type locality along the Matama ridge of the Caribbean
slope of the Chirripó Massif. The locality has mature cloud forest that includes members of: Araceae (e.g. Philo-
dendron, Anthurium, Monstera), Begonia (Begoniaceae), palms (Arecaceae), Ericaceae, Melastomataceae, Maran-
taceae, Urticaceae (e.g. Pilea), Acanthaceae, Cyclanthaceae (e.g. Cyclanthus, Carludovica), Rubiaceae, Heliconia
(Heliconiaceae), Piperaceae (e.g. Piper), bromeliads, ferns (e.g. Cyatheaceae and no tree ferns), and bryophytes.
Humidity was at or near 100% during the time spent at the site. The specimens were found during daytime in moss
mats at 0.95–2.1 m above ground and 400–600 m from the nearest stream. One specimen was found between a
plant stem and the moss, while the others were within the moss (35–90 mm wide), some in moss in vertical parts of
the trunk and others in moss hanging off the branches. Plagiochila spp was the most frequent bryophyte in the
moss mats. The temperature ranged from 16–17 °C within moss mats and 17–18 °C in the air. Three specimens
were found in the same tree.
Etymology. The species was discovered near the terminus of the Fila de Matama, a large mountain ridge that
arises as a part of Cerro Chirripó, the highest mountain in Costa Rica. The scientific name is a noun in apposition.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 49
Oedipina nimaso sp. nov.
Nimaso Worm Salamander
Figure 6
Holotype. UCR 8391, a subadult male from Cerro Nimaso, Prov. Limón, Costa Rica, 1093 m, collected by D. Rob-
inson, Federico Bolaños, and Gilbert Barrantes on April 14, 1984.
Diagnosis. A small, extremely slender member of Oedipina (Oedopinola), based on having greater than 14 but
fewer than 20 trunk vertebrae (García-París & Wake 2000; McCranie et al. 2008), distinguished from other mem-
bers of that clade by the combination of its small size, slender habitus, its long pointed snout and very narrow hands
and feet with pointed digital tips. Distinguished from Costa Rican and Panamanian members of the clade as fol-
lows: from O. carablanca by smaller size, very narrow pes (vs. very broad and webbed in O. carablanca) and
manus relative to SVL with reduced numbers of phalanges (0-1-2-1 manus vs. 1-2-3-2 in O. carablanca; 0-1-2-1-1
pes vs. 1-2-3-(2,3)-2 in O. carablanca) and little white dorsal pigment vs. extensive white pigment on head and
body in O. carablanca; from O. parvipes and O. maritima by narrower and more syndactylous hands and feet,
rounded snout and relatively large and numerous maxillary teeth (max 8 in O. maritima, fewer than 5 in Panama-
nian O. parvipes); from O. alleni in being much smaller and less robust with much narrower pes and shorter digits,
and in having more maxillary teeth (20 vs. 5 or fewer in O. alleni); from O. savagei by being less robust and in hav-
ing shorter limbs (limb interval 9.5 vs. less than 7 in O. savagei) and narrower pes (1.2 vs. 1.9 in O. savagei), and
in lacking white pigment on the back of the head and a dorsal stripe on the trunk; from O. fortunensis by having
shorter limbs (limb interval 9.5 vs. 8 in O. fortunensis), narrower pes (1.2 mm vs. 1.7 in O. fortunensis), and a
shorter, more pointed head (SL/SG = 6.4 vs. 5.2 in O. fortunensis); and from O. complex by having a longer tail
(SVL/TL less than 0.73 vs. 0.89 in O. complex), broader head (SVL/HW 8.6 vs. 10.2 in O. complex) and narrower
pes with a long, pointed third toe (vs. short rounded toe in O. complex).
Description. A diminutive, slender species compared to other Oedopinola. Sole specimen, the holotype, has a
SL of 30.8 mm. Holotype very slender (Fig. 6) with narrow head (SL/HW 8.6) and rather long snout (SL/SG = 6.4)
that is more rounded than pointed. Eyes small and only slightly protuberant. Limbs long and slender (SL/HLL =
4.5) and the right hindlimb is missing. Manus and pes flat and very narrow (SL/FW = 25.7), with digits poorly
defined and fused together. Longest digit with long, sharply pointed tip that is slightly bent in a preaxial direction.
Relatively numerous maxillary teeth relatively large and single premaxillary tooth is long and hooked, suggesting
that individual is near or at sexual maturity.
FIGURE 6. A) Dorsal and B) ventral views of preserved holotype (UCR 8391) of Oedipina nimaso. C) Dorsal view of left
hand and D) left foot of holotype. Photos A and B by Humberto Lezama, C and D by A. García-Rodríguez. The bar in hand and
feet are 1 mm length.
Measurements (in mm), limb interval and tooth counts of the male holotype (Table 2). HW 3.6, SG 4.8,
HD 1.6, EW 0.4, EL 1.1, ES 1.3, ED 0.7, IC 1.0, IO 1.2 , length of groove extending posteriorly from eye 0.8, dis-
tance between nuchal groove and gular fold 1.4, SF 7.3, IN 1.0, external naris to snout 0.4, SP 0.3, SL 30.8, SAV
26.6, AX 19.8, LI 9.5, TL 42.0 (tail broken at tip, only slightly longer in life), tail width at base 2.1, tail depth at
base 1.8, FLL 6.2, HLL 6.9, HAW 1.0, FW 1.2, T5 0.8, T3 1.1, parotoid width 1.4, parotoid length 2.9, nostril
diameter 0.6. Number of teeth: PMT 1, MT 9/11, VT 6/6.
Molecular phylogenetics
Two of the new species, Bolitoglossa splendida and B. kamuk, are part of the B. subpalmata clade (BS=100,
PP=1.0), comprising B. subpalmata, B. pesrubra, B. bramei, B. gomezi, B. gracilis, and B. tica (Fig. 7). This clade
corresponds to the B. subpalmata species group of Parra-Olea et al. (2004) and includes additional species not
described at the time. Bolitoglossa kamuk is strongly supported as the sister taxon of B. pesrubra (BS=92,
PP=0.99). Bolitoglossa tica and B. subpalmata are supported as sister species (BS=96, PP=0.97), while relation-
ships between other members of the clade, including B. splendida, are not resolved. The recently described B.
gomezi (Wake et al. 2007), which previously lacked molecular data, is a member of this group based on the
mtDNA phylogeny. A group of several species (B. epimela, B. cerroensis, B. marmorea, and B. minutula) is
resolved as the sister clade of the B. subpalmata clade with strong support (BS=100, PP=0.90). Bolitoglossa
aureogularis is the sister species of the recently described B. robinsoni (Bolaños & Wake 2009) with strong support
(BS=100, PP=1.0), based on a single specimen identified as B. robinsoni from Valle del Silencio (Fig. 7). Bolito-
glossa compacta, B. nigrescens, and B. sombra form a clade that is strongly supported in the Bayesian results
(PP=0.95), but not in the likelihood results (BS=42). Bolitoglossa compacta was included in the B. adspersa spe-
cies group of Parra-Olea et al. (2004), while B. nigrescens was included in the schizodactyla species group. These
species have few members of their species groups represented in our phylogeny, which focuses primarily on mon-
tane Talamancan taxa. The sample of B. sombra is nearly identical in sequence to both a newly sequenced sample
of B. nigrescens from Tarbaca, Prov. San José, Costa Rica (UCR 20539) (GTR distance of 0.002 for 16S and 0.003
for cyt b) and a sample of B. sombra from western Panama (CH 7478) (GTR distance of 0 for 16S and 0.001 for cyt
b). The Bayesian and ML results conflict in the placement of the B. compacta-B. nigrescens and B. aureogularis
clades. In the Bayesian results, the B. compacta-B. nigrescens clade is the sister group of B. aureogularis and B.
robinsoni (PP=0.95). In the ML results, the B. aureogularis-B. robinsoni clade is sister to the B. subpalmata and B.
epimela clades (BS=96), while the B. compacta-B. nigrescens clade is sister to all other Costa Rican salamanders
included in the analysis. Additional sequence data, as well as an expanded taxon set that includes more members of
the adspersa and schizodactyla species groups, may be needed to resolve the relationships between these clades.
Genetic distances between species included in our phylogenetic analysis are given in Table 3.
The sequenced sample of Nototriton matama is part of a clade containing other members of the N. picadoi
group (Papenfuss & Wake 1987; García-París & Wake 2000), including N. saslaya (Köhler 2002), with strong sup-
port (BS=97, PP=97), and is closely related to N. picadoi, N. gamezi and N. abscondens (Fig. 8). The relationships
between N. matama, N. picadoi, N. abscondens and N. gamezi are not resolved in the mtDNA phylogeny. The
genetic distances between members of the N. picadoi group are rather small. The largest genetic distances within
the group are between N. saslaya and N. picadoi for 16S (GTR distance=0.034) and between N. abscondens and N.
guanacaste for cyt b (0.043); a cyt b sequence was not available for N. saslaya. The smallest divergence within the
clade is between N. matama and N. picadoi (GTR distance=0.006 for 16S and 0.011 for cyt b). Genetic distances
between species of Nototriton included in our phylogenetic analyses are given in Table 4.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 51
52 · Zootaxa 3309 © 2012 Magnolia Press BOZA-OVIEDO ET AL.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 53
FIGURE 7. Phylogeny of mtDNA data for Bolitoglossa constructed using Bayesian analysis. Maximum likelihood bootstrap
values from RAxML analysis shown above branches, Bayesian posterior probabilities (multiplied by 100) shown below
branches. Support values below 50 not shown.
Discussion
Taxonomic comments and comparisons. Bolitoglossa is the largest and most widespread plethodontid genus,
with about 120 described species. Its known geographic distribution occupies nearly all of the tropical range of sal-
amanders in Central and South America. With the three new species, the genus is represented by 26 species in
Costa Rica. The eastern Talamancan region is especially rich in species of Bolitoglossa, which range nearly to the
tops of the highest mountains; for example, B. bramei, B. kamuk, B. pygmaea, and B. robinsoni have been found
above 3000 m elevation. Until now, these Talamancan species have generally been represented by few specimens.
Further complicating matters is the fact that certain taxa have lacked tissue samples. In this study we present a phy-
logenetic hypothesis based on mitochondrial DNA sequences, and there are some surprises. As anticipated, the B.
subpalmata group is widely distributed in eastern Costa Rica, and here we add two new species, B. splendida and
B. kamuk. The former is represented by a single individual, but it has a distinctive, unique color pattern and we also
have tissue that shows how genetically distinctive the species is within the group. The latter species, B. kamuk, is
less distinctive; the three individuals sampled for mtDNA are nearly identical and together they form a clade that is
sister to the remaining samples of B. pesrubra. In the phylogeny presented, B. pesrubra-B. kamuk, B. bramei, B.
gomezi, B. splendida and a clade comprising ((B. tica-B. subpalmata)(B. gracilis)) form essentially a polytomy.
These taxa are mainly allopatric, as far as current knowledge is concerned. While the known distributions of B.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 55
bramei and B. gomezi closely approach each other near the Costa Rica-Panama border, B. gomezi has generally
been found at lower elevations (1170–2120 m) than B. bramei (1900–3200 m). To the northwest, along the cordil-
leran spine, B. gomezi and B. kamuk are sympatric at Potrero Grande. Although the distribution of B. kamuk is
poorly known, it is replaced further to the northwest by B. pesrubra, which occurs as far to the southeast as Cerro
Chirripó Grande. Bolitoglossa pesrubra then persists to the northwest in the region known as Cerro de la Muerte
and neighborhood until being replaced at lower elevation (about 2500 m) by B. tica. We suspect B. tica and B. gra-
cilis, which occurs at even lower elevation (to 1200 m) to the east of the main cordilleran spine, may occur in sym-
patry between the Río Reventazón valley in the vicinity of Tapantí and El Empalme. Finally, B. subpalmata occurs
in the Cordillera Volcánica Central and mountains to the northwest as far as Volcán Cacao. Thus, members of the B.
subpalmata group occupy much of the suitable terrain above about 1200 m from northwestern Costa Rica to west-
ern Panama.
Our phylogeny of Bolitoglossa includes several species for which no published sequence data exists. We
assign UCR 20532 to B. compacta based on its red-orange blotched dorsal color pattern; this specimen is from
Valle del Silencio, an area where B. compacta is known to occur (Hanken et al. 2005). Bolitoglossa robinsoni is
known from the type locality at Cerro Echandi on the Costa Rica-Panama border, with several other morphologi-
cally divergent populations along the continental divide near the border tentatively assigned to this species. These
divergent populations, including Valle del Silencio, the farthest locality from Cerro Echandi, may belong to a sepa-
rate species from B. robinsoni (Bolaños & Wake 2009); the Valle del Silencio specimen is here assigned to B. rob-
insoni based on overall morphology, pending the acquisition of topotypic material. We assigned UCR 20539, from
Tarbaca, Aserrí, San José to B. nigrescens because B. nigrescens is the only large, black Bolitoglossa that is known
from the area (Hanken et al. 2005; García-París et al. 2008). We assigned another specimen (CH 7478) used in our
phylogenetic analysis to B. sombra, based on both geographic distribution and its similarity in mtDNA sequence to
B. sombra, although we lack morphological data for this specimen. Finally, we tentatively assign several samples
collected on the trips to Cerro Kamuk, Cerro Amuo and Tres Colinas to B. gomezi, based on external morphologi-
cal similarity to other specimens of B. gomezi and because these specimens cannot be assigned to B. bramei or B.
robinsoni. As with B. robinsoni, definitive identification of these specimens will require additional material from
the type locality of B. gomezi.
A surprise from our molecular analysis was the discovery of a previously unknown clade including, at present,
two taxa, B. aureogularis and B. robinsoni. This well supported clade (BS=100, PP=0.85) is either the sister group
(in the ML results) to the two clades (B. subpalmata and B. epimela) previously considered to constitute the Central
American subclade of Bolitoglossa (Eladinea) (Parra-Olea et al. 2004), or the sister clade (in the Bayesian results)
to the largely South American subclade of Bolitoglossa (Eladinea) here represented by B. compacta, B. nigrescens,
and B. sombra, (Parra-Olea et al. 2004; Hanken et al. 2005). The known geographic distributions of the two spe-
cies in this new clade are separated, B. aureogularis to the north and west in eastern Costa Rica, and B. robinsoni to
the south and east in the border region and into western Panama. The larger and more robust B. robinsoni is easily
distinguished from the smaller and more slender B. aureogularis by coloration and body form.
While our phylogeny supports higher species diversity in the Talamanca region than was previously known, it
also calls into question the distinctiveness of B. sombra. Although B. sombra was originally distinguished from B.
nigrescens based on morphological differences (more slender trunk, shorter limbs, and fewer teeth compared to B.
nigrescens), these differences were very slight (shown in Fig. 3–4 of Hanken et al. 2005). In our mtDNA phylog-
eny, a sample of B. sombra (MVZ 225875) from the type locality near Las Tablas, Costa Rica and another from
Panama are nearly identical in sequence to the available sample of B. nigrescens. The lack of sequence divergence
between the two taxa, coupled with the lack of substantial morphological differences, does not support the status of
B. sombra as a distinct species. The type specimen of B. nigrescens is a subadult, and no additional topotypic spec-
imens are available for the species. Furthermore, we have not had the opportunity to examine the Panamanian spec-
imen of B. sombra whose sequence we used in our phylogenetic analysis. We refrain from declaring B. sombra to
be a synonym of B. nigrescens at present, pending discovery and study of topotypic material for B. nigrescens.
Nototriton includes fifteen described species presently arranged in three species groups: barbouri (N. bar-
bouri, N. brodiei, N. lignicola, N. limnospectator, N. picucha, N. stuarti), picadoi (N. abscondens, N. gamezi, N.
guanacaste, N. major, N. saslaya and N. matama) and richardi (N. richardi, N. tapanti) (García-París & Wake
2000; Köhler 2002). Townsend et al. (2010) described N. tomamorum but refrained from assigning it to an existing
species group. The known distribution of the barbouri group is restricted to eastern Guatemala and Honduras
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 57
a subclade proposed as the sister taxon of O. elongata + O. carablanca, McCranie & Townsend 2011) are known
only from central/northern Honduras and to the north as far as Chiapas, southern Mexico. Based on their small geo-
graphic ranges and distance from Costa Rica, all are unlikely to be conspecific with, or even related closely to O.
nimaso. The new taxon is readily distinguished by its minute size from species that reach much larger sizes (O.
elongata to over 66 mm SL, O. tomasi to 63 mm SL, O. gephyra to over 58 mm SL, O. alleni to over 57 mm SL, O.
carablanca to over 54 mm SL, O. parvipes to over 52 mm SL; Brame 1968, McCranie 2006). All of these species
have a broader manus and pes than O. nimaso. Because sample sizes are so small, statistical comparisons are effec-
tively impossible, but if we use molecular studies of the subgenus (e.g., García-París & Wake 2000, McCranie &
Townsend 2011) for comparisons, species ranges are typically very small, and thus any species from west of the
cordilleran divide in Costa Rica/Panama are unlikely on distribution alone to be conspecific with O. nimaso; in
addition, they are all larger and more robust. If we restrict our consideration to species that are known to occur
north and east of the cordilleran divide, the candidates are O. fortunensis, O. maritima, O. complex, and O. parvi-
pes. Only the latter two are represented by good sample sizes, but both are likely to be species complexes, or
agglomerations of morphologically similar forms that may not even be close relatives (based on unpublished mor-
phological studies of DBW). Brame (1968) presents plots of head width against SL, maxillary tooth totals against
SL, hind limb length against SL, and foot width against SL for his samples identified as O. complex and O. parvi-
pes. The single specimen of O. nimaso falls approximately on the same regression as O. complex with respect to
foot width, but the specimen of O. complex closest in size to the holotype is much larger, and O. parvipes has
broader feet. The limb length of the holotype of O. nimaso is roughly on the regression line for O. parvipes but
above (longer) that for O. complex. Total numbers of maxillary teeth are much higher than for either O. complex of
roughly comparable size, or for all sizes of Panamanian representatives of O. parvipes. Head widths appear to be
comparable in the three species.
McCranie and Townsend (2011) present the most complete molecular phylogeny for Oedopinola, including 10
of the 12 species. The gephyra subclade has been discussed above and on morphological grounds O. nimaso is
readily excluded from this northern subclade. A second subclade includes O. alleni and O. savagei, morphologi-
cally differentiated taxa from the Pacific coast and nearby slopes, and we think it unlikely that O. nimaso is related
to them. The final subclade includes O. complex (topotypic material), O. maritima (topotypic material), and O.
parvipes (from central Panama, unlikely to be conspecific with topotypic material, which is unavailable for molec-
ular studies). We think it likely, on geographic as well as morphological grounds, that O. nimaso will prove to be a
member of this subclade. O. fortunensis (the only taxon other than O. nimaso excluded from the molecular analy-
sis) might be a member either of this subclade or the alleni - savagei subclade.
Despite having only a single specimen, we decided that the most responsible course of action is to recognize
O. nimaso as a new taxon. Both specimens of O. fortunensis have a unique color pattern (see Köhler et al. 2007,
AmphibiaWeb) and the holotypes (the only specimens for which measurements are available) differ in important
morphological traits, so there is no basis for assigning the single specimen to O. fortunensis. To assign it to either
O. complex or O. parvipes seems unwise, given that the unique specimen does not fit into either of these complexes
with respect to morphology, and especially in light of the likelihood that both are species complexes (or, most
likely, taxa of convenience, given the large geographic ranges of each in a subgenus noted for the narrow, restricted
geographic distributions of the included species).
Biogeography. The results of our fieldwork and phylogenetic analyses shed light on the biogeography of the
Talamancan region. Based on present knowledge of the geographic distributions of salamanders in southern Cen-
tral America, Bolitoglossa (Eladinea) reaches its northern limit in northwestern Costa Rica, with B. subpalmata
and B. robusta on Volcán Cacao (Savage 2002). The subgenus Eladinea thus does not cross the Nicaraguan depres-
sion, and is restricted to the Chorotega and Chocó blocks of Central America (Marshall 2007) as well as northern
South America. Within the Cordillera de Talamanca, if we restrict our discussion to elevations above 1500 m, the
majority of known salamanders are members of the subpalmata and epimela species groups of Eladinea (Parra-
Olea et al. 2004); both of these species groups are restricted to the Chorotega block. The only salamanders that
have been found above 1500 m in the Cordillera de Talamanca from other species groups are B. compacta (in the
adspersa group), B. aureogularis and B. robinsoni, and the large, black species (such as B. nigrescens, B. robusta,
and B. sombra) that are members of the schizodactyla group. Parra-Olea et al. (2004) placed B. diminuta in the
schizodactyla species group based on partial mtDNA sequences, while Savage (2002) stated that it probably
belongs to the subpalmata species group. The description of two new species (B. kamuk and B. splendida) within
Acknowledgments
S. Rovito was partially supported by the National Science Foundation AmphibiaTree grant (EF-0334939) and an
NSF Bioinventory grant (DEB 1026393). We thank Javier Guevara from the Ministerio de Ambiente, Energía y
Telecomunicaciones who provided collecting and export permits and J. Townsend for providing sequence data for
Nototriton. The trips to different sites of the southeastern Talamanca were financed by Vicerrectoría de Investi-
gación of Universidad de Costa Rica (UCR), Instituto Nacional de Biodiversidad de Costa Rica (INBio), NSF grant
EF-0334939, and Baseline Tools for Management of PN La Amistad (No. 15027) financed by the Darwin Initiative
to Alex Monro (Natural History Museum, London, England) in collaboration with Nelson Zamora (INBio). We
also thank the Spanish Government by its “Agencia Española de Cooperación Internacional y de Desarrollo” under
the funds D/027406/09 for 2010 and D/033858 for 2011 to Universidad de Las Palmas de Gran Canaria and Uni-
versidad de Costa Rica. Thanks to botanists Carlos Morales (Universidad de Costa Rica), Alexander Rodríguez,
Daniel Solano, Daniel Santamaría, Nelson Zamora, Francisco Morales (all from INBio), Barry Hammel (Missouri
Botanical Garden) and Alex Monro. Humberto Lezama helped with O. nimaso pictures. We would also like to
thank the many people that helped before, during, and after the field trips, especially Carlos Godínez, William
Gamboa, Ruth Delgado, Carlos Solano, and Frank González.
References
AmphibiaWeb (2011) Information on amphibian biology and conservation. Berkeley, California: http://amphibiaweb.org/
(Accessed: November 21, 2011).
Bolaños, F. & Wake, D.B. (2009) Two news species of montane web-footed salamanders (Plethodontidae: Bolitoglossa) from
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 59
the Costa Rica-Panamá border region. Zootaxa, 1981, 57–68.
Brame, A.H. Jr. (1968) Systematics and evolution of the Mesoamerican salamander genus Oedipina. Journal of Herpetology, 2,
1–64.
Edgar, R.C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinfor-
matics, 5, 1–19.
Excoffier, L., Laval, G., & Schneider, S., (2005) Arlequin (Version 3.0): an integrated software package for population genetics
data analysis. Evolutionary Bioinformatics Online, 1, 47–50.
García-París, M. & Wake, D.B. (2000) Molecular phylogenetic analysis of relationships of the tropical salamander genera
Oedipina and Nototriton, with descriptions of a new genus and three new species. Copeia, 2000, 42–70.
García-París, M., Parra-Olea, G., & Wake, D.B. (2008) Description of a new species of the Bolitoglossa subpalmata group
(Caudata: Plethodontidae) from Costa Rica. Herpetological Journal, 18, 23–31.
Good, D.A. & Wake, D.B. (1993) Systematic studies of the Costa Rican moss salamanders, genus Nototriton, with descriptions
of three new species. Herpetological Monographs, 7, 131–159.
Hanken, J., Wake, D.B., & Savage, J.M. (2005) A solution to the large black salamander problem (genus Bolitoglossa) in Costa
Rica and Panamá. Copeia, 2005, 227–245.
Hasegawa, M. Kishino, H., & Yano, T. (1985) Dating of human-ape splitting by a molecular clock of mitochondrial DNA.
Journal of Molecular Evolution, 22, 160–174.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–755.
Kimura, M. (1980) A simple method for estimating evolutionary rates of base substitutions through comparative studies of
nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
Köhler, G. (2002) A new species of salamander of the genus Nototriton from Nicaragua (Amphibia: Caudata: Plethodontidae).
Herpetologica, 58, 205–210.
Köhler, G., Ponce, M., & Batista, A. (2007) A new species of worm salamander (genus Oedipina) from Fortuna, western central
Panama (Amphibia, Caudata, Plethodontidae). Senckenbergiana Biologica, 87, 213–217.
Leviton, A.E., Gibbs, R.H., Jr., Heal, E., & Dawson, C.E. (1985) Standards in herpetology and ichthyology: Part I. Standard
symbolic codes for institutional resource collections in herpetology and ichthyology. Copeia, 1985, 802–832.
Marshall, J.S. (2007) The geomorphology and physiographic provinces of Central America. In: Bundschuh, J. and Alvarado,
G.E. (Eds), Central America: Geology, Resources and Hazards. Taylor & Francis, Oxford, UK, pp. 75–122.
McCranie, J.R. (2006) New species of Oedipina (Amphibia: Caudata) from Parque Nacional El Cusuco, northwestern Hondu-
ras. Journal of Herpetology, 40, 291–293.
McCranie, J.R. & Townsend, J.H. (2011) Description of a new species of worm salamander (Caudata: Plethodontidae) in the
subgenus Oedipinola from the central portion of the Cordillera Nombre de Dios, Honduras. Zootaxa, 2990, 59–68.
McCranie, J.R., Vieites, D.R., & Wake, D.B. (2008) Description of a new divergent lineage and three new species of Honduran
salamanders of the genus Oedipina (Caudata, Plethodontidae). Zootaxa, 1930, 1–17.
Moritz, C., Schneider, C.J., & Wake, D.B. (1992) Evolutionary relationships within the Ensatina eschscholtzii complex confirm
the ring species interpretation. Systematic Biology, 41, 273–291.
Nylander, J.A.A. (2004) MrModeltest. Program distributed by the author. Evolutionary Biology Center, Uppsala University,
Uppsala, Sweden.
Nylander, J.A.A., Wilgenbusch, J.C., Warren, D.L., & Swofford, D.L. (2008) AWTY (Are We There Yet?): a system for graph-
ical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics, 24, 581–583.
Palumbi, S.R., Martin, A.P., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The Simple Fool's Guide to PCR.
Special Publication, Department of Zoology, University of Hawaii, Honolulu, Hawaii, USA.
Papenfuss, T.J. & Wake, D.B. (1987) Two new species of plethodontid salamanders (genus Nototriton) from Mexico. Acta Zoo-
logica Mexicana (ns), 21, 1–16.
Parra-Olea, G., García-París, M. & Wake, D.B. (2004) Molecular diversification of salamanders of the tropical American genus
Bolitoglossa (Caudata: Plethodontidae) and its evolutionary and biogeographical implications. Biological Journal of the
Linnean Society of London, 81, 325–346.
Savage, J.M. (2002) The Amphibians and Reptiles of Costa Rica. The University of Chicago Press, Chicago, U.S.A. 934 pp.
Stamatakis, A. (2006) RAxML-VI-HPC: Maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed
models. Bioinformatics, 22, 2688–2690.
Tamura, K. & Nei, M. (1993) Estimating the number of nucleotide substitutions in the control region of mitochondrial DNA in
humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.
Tavaré, S. (1986) Some probabilistic and statistical problems on the analysis of DNA sequences. Lectures on Mathematics in
the Life Sciences, 17, 57–86.
Townsend, J.H., Butler, J.M., Wilson, L.D., & Austin, J.D. (2010) A distinctive new species of moss salamander (Caudata:
Plethodontidae: Nototriton) from an imperiled Honduran endemism hotspot. Zootaxa, 2434, 1–16.
Townsend, J.H., Medina-Flores, M., Murillo, J.L., & Austin, J.D. (2011) Cryptic diversity in the Chortís Highland moss sala-
manders (Caudata: Plethodontidae: Nototriton) revealed using mtDNA barcodes and phylogenetics, with a new species
from eastern Honduras. Systematics and Biodiversity, 9, 275–287.
Wake, D.B. (1966) Comparative osteology and evolution of the lungless salamanders, Family Plethodontidae. Memoirs of the
Southern California Academy of Sciences, 9, 1–111.
NEW SPECIES OF SALAMANDERS FROM COSTA RICA Zootaxa 3309 © 2012 Magnolia Press · 61