Merck Chrom Media Portfolio PDF
Merck Chrom Media Portfolio PDF
Merck Chrom Media Portfolio PDF
Fractogel® EMD TMAE Acidic, neutral, recombinant • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1168810010
(M) media proteins and peptides from • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250540001 100 ml 1168810100
Base matrix: Methacrylate different sources, (including 5 ml (8*100 mm) 1250690001 500 ml 1168810500
• pH stability range pH 2-12, Pressure limit 8 bar
plasma proteins). DNA and virus 5L 1168815000
• Linear flow rate up to ~250 cm/h RoboColumns®:
removal, plasmid isolation.
0.2 ml (5*10 mm) 1250840001
0.6 ml (5*30 mm) 1251360001
Strong anion exchangers (AEX)
Fractogel® EMD TMAE • Particle size: 20-40 µm (S) type MiniChrom Columns: 10 ml 1168870100
Trimethylammoniumethyl
(S) media • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250570001 100 ml 1168870500
Base matrix: Methacrylate 5 ml (8*100 mm) 1250710001 500 ml 1168875000
• pH stability range pH 2-12, Pressure limit 8 bar
5L
• Linear flow rate up to ~150 cm/h (S-type) RoboColumns®:
TMAE
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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD DEAE Acidic, neutral, recombinant • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168830010
(M) media proteins and peptides from • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250580001 100 ml 1168830100
Base matrix: Methacrylate different sources, (including 5 ml (8*100 mm) 1250790001 500 ml 1168835000
• pH stability range pH 2-13
plasma proteins) and DNA 5L
• Pressure limit 8 bar RoboColumns®:
Diethylaminoethyl
removal.
• Linear flow rate up to 170 cm/h 0.2 ml (5*10 mm) 1251450001
Due to the titration behaviour, 0.6 ml (5*30 mm) 1251510001
DEAE
Weak anion exchangers (AEX)
Fractogel® EMD DMAE Strong acidic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168840010
(M) media
Dimethylaminoethyl
proteins (e.g. plasma proteins), • Protein binding capacity about 100 mg BSA/ml of gel 1 ml (8*20 mm) 1250590001 100 ml 1168840100
Base matrix: Methacrylate peptides, and nucleic acids. 5 ml (8*100 mm) 1250800001 500 ml 1168840500
• pH stability range pH 2-13
5L 1168845000
Due to the titration behavior, RoboColumns®:
DMAE
• Pressure limit 8 bar
the ion exchange media can be • Linear flow rate up to 170 cm/h 0.2 ml (5*10 mm) 1251460001
used from pH 2 up to pH 8.5. 0.6 ml (5*30 mm) 1251520001
Blood fractionation.
Eshmuno® S media Strong basic and neutral • Particle size d50: 85 µm MiniChrom Columns: 10 ml 1200780010
Base matrix: Hydrophilic proteins (e.g. antibodies) and • P rotein binding capacity 135-195 mg lysozyme/ml of 1 ml (8*20 mm) 1250640001 100 ml 1200780100
Polyvinyl Ether peptides, designed specifically settled resin, IgG Dynamic Capacity ≥ 60 mg/ml 5 ml (8*100 mm) 1250730001 500 ml 1200780500
for highly productive (2 min residence time, 10% BT) 5L 1200785000
RoboColumns®:
downstream bioprocessing.
• pH stability range (working): 2-12 0.2 ml (5*10 mm) 1251320001
Superior binding capacity in • pH stability range (CIP): 0-14 0.6 ml (5*30 mm) 1251400001
Strong cation exchangers (CEX)
Eshmuno® CPX media Intermediate or polishing • Mean particle size: 50 µm MiniChrom Columns: 10 ml 1200830010
Base matrix: Hydrophilic purification of monoclonal • P rotein binding capacity 110 mg plgG/ml settled resin 1 ml (8*20 mm) 1251560001 100 ml 1200830100
Polyvinyl Ether antibodies. (4 min residence time, 5% BT) 5 ml (8*100 mm) 1251570001 500 ml 1200830500
5L 1200835000
High aggregate removal • pH stability (working): 2-12
RoboColumns®:
efficiency. • pH range (CIP): 0-14 0.2 ml (5*10 mm) 1251580001
Excellent IgG dynamic binding • Pressure limit 8 bar 0.6 ml (5*30 mm) 1251590001
capacity. • L inear flow rate up to 500cm/h
(<3.0 bar net pressure)
Wide pH and conductivity
operating window.
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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD SO3- Strong basic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168820010
(M) media proteins (e.g. antibodies) and • Protein binding capacity about 130 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250610001 100 ml 1168820100
Base matrix: Methacrylate peptides. 5 ml (8*100 mm) 1250720001 500 ml 1168820500
• pH stability range pH 1-13
5L 1168825000
• Pressure limit 8 bar RoboColumns®:
• Linear flow rate up to ~200 cm/h 0.2 ml (5*10 mm) 1250900001
0.6 ml (5*30 mm) 1251390001
Sulfoisobutyl
with Fractogel® EMD SO3 (M)
media: CHRPC18821
SO3
10 cm (inner diameter) CHRPC18822
Strong cation exchangers (CEX)
Fractogel® EMD SO3- Strong basic and neutral • Particle size: 20-40 µm 10 ml 1168900010
(S) media proteins (e.g. antibodies) and • Protein binding capacity about 150 mg lysozyme/ml of gel 100 ml 1168900100
Base matrix: Methacrylate peptides. 500 ml 1168900500
• pH stability range pH 1-13
5L 1168905000
• Pressure limit 8 bar
• Linear flow rate up to ~80 cm/h
Fractogel® EMD Strong basic and neutral • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1148940010
SE Hicap (M) media proteins (e.g. antibodies) and • Protein binding capacity about 160 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250600001 100 ml 1148940100
Base matrix: Methacrylate peptides. 5 ml (8*100 mm) 1250810001 500 ml 1148940500
• pH stability range pH 1-13
5L 1148945000
• Pressure limit 8 bar RoboColumns®: 1251470001
0.2 ml (5*10 mm)
Sulfoethyl
Fractogel® EMD COO- Basic and neutral proteins and • Particle size: 40-90 μm MiniChrom Columns: 10 ml 1168860010
(M) media peptides. • Protein binding capacity about 100 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250620001 100 ml 1168860100
Base matrix: Methacrylate 5 ml (8*100 mm) 1250820001 500 ml 1168860500
Weak cation exchangers
used from pH 6 up to pH 12. • Linear flow rate up to~300 cm/h 0.2 ml (5*10 mm) 1251480001
0.6 ml (5*30 mm) 1251540001
COO-
(CEX)
Eshmuno® HCX media Direct capture and intermediate • Mean particle size: d50 , 85 µm MiniChrom Columns: 10 ml 1200870010
sulfo + carboxy + phenyl
salt-tolerant (multi-mode
Base matrix: Hydrophilic polishing from clarified feed • P rotein binding capacity (5 min residence time, 10 % 1 ml (8*20 mm) 1250660001 100 ml 1200870100
Polyvinyl Ether stocks. breakthrough) ≥ 50 mg pIgG /ml settled resin 5 ml (8*100 mm) 1250750001 500 ml 1200870500
5L 1200875000
High dynamic binding • pH stability range (working): 2-12 RoboColumns®:
CEX High
media)
capacity at high conductivity • pH stability range (CIP): 2-14 0.2 ml (5*10 mm) 1251340001
applications. • Pressure limit 8 bar 0.6 ml (5*30 mm) 1251420001
• Linear flow rate up to 1000 cm/h (<2.5 bar net pressure)
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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Hydrophobic interaction Fractogel® EMD Phenyl Weak hydrophobic • Particle size: 20-40 µm 10 ml 1161970010
chromatography (HIC) (S) media chromatography support for • Protein binding capacity about 20 mg ovalbumin/ml of gel 100 ml 1161970010
Base matrix: Methacrylate purification of proteins and 500 ml 1161970500
• pH stability range pH 2-12
antibodies, membrane proteins.
• Pressure limit 8 bar
Phenyl
• Linear flow rate up to 80 cm/h
Fractogel® EMD Chelate Various proteins, e.g. histidine- • Particle size: 40-90 µm MiniChrom Columns: 10 ml 1103380010
Immobilized metal affinity
(M) media
chromatography (IMAC)
tailed recombinant proteins or • Protein binding capacity about 60 mg lysozyme/ml of gel 1 ml (8*20 mm) 1250630001 100 ml 1103380100
Iminodiacetic acid
Base matrix: Methacrylate proteins with exposed histidine 5 ml (8*100 mm) 1250830001 250 ml 1103380250
residues. •M etal ion binding capacity about 75 μmol copper/ml 500 ml 1103380500
of gel RoboColumns®: 5L 1103385000
The metal ion can coordinate to
• pH stability range pH 1-12 0.2 ml (5*10 mm) 1251490001
Non-bonding electron pairs of
• Pressure limit 8 bar 0.6 ml (5*30 mm) 1251550001
the amino acid side chains.
• Linear flow rate up to ~240 cm/h
Eshmuno® A media High capacity Protein A • Particle size: ~50 µm MiniChrom Columns: 10 ml 1200890010
Merck Millipore proprietary
Base matrix: affinity chromatography • P rotein Binding capacity 40-55mg/ml at 3-6 min RT and 1 ml (8*20 mm) 1251600001 100 ml 1200890100
ligand recombinantely
resin for the purification of 5% breakthrough for mAbs 5 ml (8*100 mm) 1251610001 500 ml 1200890500
Fc-containing proteins, 5L 1200895000
• pH stability range 2.5-8.0 RoboColumns®:
including but not limited to 1251620001 10 L 1200899010
• Pressure limit 8 bar 0.2 ml (5*10 mm)
monoclonal antibodies 1251630001
• Linear flow rate >500 cm/hr 0.6 ml (5*30 mm)
ProSep® Ultra Plus Affinity purification of mAbs, • Particle size: 60 µm MiniChrom Columns: 2 ml 175118822
media rSPA = recombinant designed for cost effective, 1 ml (8*20 mm) 1250670001 10 ml 175118824
Protein A affinity chromatography
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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Fractogel® EMD BioSEC Separation of proteins in the • Particle size: 20-40 µm 150 ml 1103170150
(S) media range between 5kDa and 250 ml 1103170250
chromatography
• pH stability range pH 1-12
Modified pore
Size exclusion
structure
(SEC)
PharmPrep® P sorbent Small peptides (insulin), APIs, • Spherical high performance sorbent Ready to use columns for method 10, 100, 1000, 5000g*
Base matrix: Spherical antibiotics, intermediates. • Mean pore size: 10 nm development
PharmPrep® P sorbent 100
particles of silica Scout columns 250x4.6 mm
• Pressure PharmPrep® P Si <25 bar (10 µm), 10 bar RP-18e, 10 μm 1.19995
• P ressure PharmPrep® 100 RP-18e <40 bar PharmPrep® P sorbent 100 RP-18e,
(10 µm) PharmPrep® P sorbent 100
10 μm 1205710001
RP-18e, 10 µm, 20 µm
1.19996
Si 100, 10 µm, 20 µm
RP-18e, 20 μm
PharmPrep® P sorbent 100 RP-18e,
20 μm 1205720001 PharmPrep® P sorbent
Si 100, 10 μm 1.19681
Ready to use columns for
preparative chromatography PharmPrep® P sorbent
HIBAR® prepacked columns Si 100, 20 μm 1.19682
250x25 mm
Normal phase/Reversed phase chromatography and Silica gel
LiChroprep® sorbent APIs, antibiotics, steroides, • Irregular particles Ready to use columns for preparative On demand 100, 500, 1000g*
Base matrix: Irregular intermediates. • 1 5-25 µm, 25-40 µm, 40-63 µm chromatography HIBAR® prepacked
particles of silica LiChroprep® sorbent
• Mean pore size: 10 nm columns CN 40-63 µm 1.13959
250x25 mm, 250x50 mm
LiChroprep® sorbent
RP-18e, 15-25 µm 1.13901
LiChroprep® sorbent
RP-18e, 25-40 µm 1.09303
LiChroprep® sorbent
RP-8, CN, NH2, Diol
LiChroprep® sorbent
RP-8, 40-63 µm 1.09362
LiChroprep® sorbent
Diol, 40-63 µm 1.13973
LiChroprep® sorbent
NH2, 40-63 µm 1.13974
LiChroprep® sorbent
Si 60, 15-25 µm 1.09336
LiChroprep® sorbent
Si 60, 25-40 µm 1.09390
LiChroprep® sorbent
Si 60, 40-63 µm 1.13905
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Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Silica gel Natural ingredients, vitamins, • For normal phase chromatography, 500g to 400kg*
Base matrix: Irregular F&B. filtration, flash chromatography Silica gel Si 60, 15-40 µm 1.13905
Normal phase/Reversed phase chromatography and Silica gel
particles of silica • Particle size: Silica gel Si 60, 40-63 µm 1.09385
200 - 500 μm
Si 60
63 - 200 μm Silica gel Si 60, 63-100 µm 1.15101
40 - 63 μm Silica gel Si 60, 63-200 µm 1.07734
15 - 40 μm Silica gel Si 60, 200-500 µm 1.07733
• Mean pore size: 6 nm
• pH (10 % aq. Solution) 6.5 - 7.5
Aluminium Oxide Intermediates • For normal phase chromatography 1, 2, 5, 50kg*
For further details, please see our product specific brochures and Data Sheets * cat. no. needs to be complemented by the 4-digit volume indication: 10g: 0010; 100g: 0100; 1000g: 1000; 5000g: 5000 7
Media Type Product Description Ligand Application Product Characteristics Prepacked Columns Catalog No. Bulk Media Catalog No.
Chromabolt® Prepacked, prevalidated See resin for resin characteristics. Chromabolt® columns prepacked
prepacked columns chromatography columns, All columns are packed in a ISO 8 Clean Room using with Fractogel® EMD TMAE (M): CHRPC18891
with Merck Millipore resins automated packing and sanitizing equipment. This ensures 10 cm (inner diameter) CHRPC18892
for early clinical stage, process a high quality reproducible and robust manufacturing 20 cm (inner diameter) CHRPC18893
Chromatography columns prepacked
see resin
conditions. 32 cm (inner diameter) CHRPC18833
Or visit: www.merckmillipore.com/offices
www.merckmillipore.com /biochromatography
Merck Millipore, the M logo, Chromabolt, Eshmuno, Fractogel, HIBAR, LiChroprep, PharmPrep and ProSep are registered trademarks of Merck KGaA, Darmstadt, Germany.
Robocolumn is a registered trademark of Atoll GmbH, Weingarten. Lit code: PF4422EN00 Version 2.0 DP SBU-11-05184 © 2015 EMD Millipore Corporation, Billerica, MA, USA. All rights reserved.