Introduction To Genomics and Proteomics - Class Notes For Biotech. 401

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Introduction to Genomics and Proteomics - Class Notes for Biotech. 401
Book · January 2013
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Introduction to Genomics and Proteomics
Class Notes for Biotech. 401
Dr. G.S. Miglani

Visiting Professor
School of Agricultural Biotechnology

Punjab Agricultural University
Ludhiana
2012
School of Agricultural Biotechnology
Punjab Agricultural University, Ludhiana
COURSE CONTENT-CUM-LECTURE SCHEDULE
Biotech. 401 Introduction to Genomics and Proteomics 3+0
Teachers - Dr G.S. Miglani

Visiting Professor
Email: miglanigs@pau.edu
COURSE CONTENT: Genomic analysis tools: large scale DNA sequencing, genetic mapping, micro-array
technologies, robotics, and bioinformatics. Structure of genomes: bacteria, yeast, nematode,
Arabidopsis, rice, zebra fish, mouse and man. High-resolution genetic analysis: genetic markers (SSR,
AFLP and SNP’s), mapping methods (family analysis, bulked segregant analysis, identity by descent,
whole genome association) and positional gene identification. Transcript profiling: EST sequencing,
SAGE, cDNA fragment analysis methods, array-based hybridization approaches. Gene characterization:
covers DNA sequence analysis, assessment of gene expression, functional genomics approaches, and
production of transgenic plants. Genomics approaches for improvement of biotic stresses, abiotic
stresses and quality characteristics in crop plants. Analysis and characterization of proteins and
metabolites: Introduction to transcriptomics, proteomics and metabolomics; proteomics approaches to
the analysis of plant proteins, protein-protein interactions, and metabolic profiling through emerging
metabolomic techniques like 2D gel electrophoresis and Mass spectrometric and computational
techniques.
LECTURE SCHEDULE
Sr. Topic
No.
1 Introduction to genomics and proteomics
2 Genomic analysis tools
3 Large scale DNA sequencing
4 Robotics and bioinformatics
5 Micro-array technologies
6 Transcript profiling: EST sequencing, SAGE, cDNA fragment analysis methods, array-based
hybridization approaches
7 Structure of genomes: bacteria, yeast, nematode, Arabidopsis, rice, zebra fish, mouse and man
8 High-resolution genetic analysis: genetic markers (SSR, AFLP and SNP’s),
9 Genetic mapping
10 Mapping methods (family analysis, bulked segregant analysis, identity by descent, whole genome
association) and positional gene identification
11 Gene characterization: DNA sequence analysis, assessment of gene expression, functional
genomics approaches, and production of transgenic plants.
12 Genomics approaches for improvement of biotic stresses, abiotic stresses and quality
characteristics in crop plants.
13 Analysis and characterization of proteins and metabolites: Introduction to transcriptomics,
proteomics and metabolomics
14 Proteomics approaches to the analysis of plant proteins, protein-protein interactions, and
metabolic profiling through emerging metabolomic techniques like 2D gel electrophoresis and
Mass spectrometric and computational techniques.
15 Recent progresses in genomics and proteomics
16 Applications of genomics and proteomics
BOOKS RECOMMENDED
1. Gupta, P.K. 2004. Biotechnology and Genomics. First edition. Rastogi Publications, Meerut.
2. Gupta, P.K. 2009. Plant Biotechnology. Rastogi Publications, Meerut.
3. Meksem, K. and Gahl, K. 2005. Handbook of Plant Genome Mapping: Genetic and Physical
Mapping. pages 233-256. Wiley-VCH.
4. Miglani, G.S. 2007. Advanced Genetics. New Delhi: Narosa Publishing House.
5. Primrose, S.B. and Twyman, R.M. 2006. Principles of Gene Manipulation and Genomics. Blackwell
Publishing, Australia.
6. Singh, B.D. 2009. Biotechnology: Expanding Horizons. Second Edition. Kalyani Publishers,
Ludhiana.
7. Singh, B.D. 2009. Plant Biotechnology. Kalyani Publishers, Ludhiana.
8. Thompson, J.D., Schaeffer-Reiss, C., and Ueffing, M. 2008. Functional Proteomics. Methods and
Protocols. Humana Press, New York.
9. Twyman, R.M. 2004. Principles of Proteomics. Taylor & Francis.
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See discussions, stats, and author profiles for this publication at: https://www.researchgate.

Introduction to Genomics and Proteomics - Class Notes for Biotech. 401

Book · January 2013

At present I have teaching assignment View project

Induced male recombination in Drosophila View project

The user has requested enhancement of the downloaded file.

Class Notes for Biotech. 401

Dr. G.S. Miglani

School of Agricultural Biotechnology

COURSE CONTENT-CUM-LECTURE SCHEDULE

Biotech. 401 Introduction to Genomics and Proteomics 3+0

Teachers - Dr G.S. Miglani

View publication stats

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