Venipuncture: Syringe Draw Procedure Principles of Medical Technology Practice 2 Laboratory Act. 6

University of Santo Tomas
Faculty of Pharmacy
Department of Medical Technology
Venipuncture
Syringe Draw Procedure
Principles of Medical Technology Practice 2
Laboratory
Act. 6
Intended Learning Outcomes:
By the end of the session, the students should:
• Explain the advantages and disadvantages of syringe
method in venipuncture.
• Analyze the step-by-step procedure in venipuncture using
a single draw syringe.
• Perform the skills learned in venipuncture by syringe
method.
Principle
• To obtain venous blood acceptable for laboratory testing
as required by a physician
Sample:
• Venous blood collected to be aliquoted into evacuated
tubes or special collection containers
Materials
Syringe, varies in size Sterile gauze or cotton
balls
Disposable needle for
syringe, 21 or 22 gauge Adhesive bandage/
medical tape
Evacuated tubes or
special collection Biohazard sharps
containers container
Tourniquet Disposable gloves
70% isopropyl alcohol  Safety glasses and mask
Transfer device
Procedures:
1. Identify the patient.
 Inpatient – ask the name of the patient, verify the bracelet name
and hospital number/requisition information
 Outpatient - ask the name of the patient, verify the bracelet
name and hospital number/requisition information, and/or ask for
a valid identification card
2. If fasting sample is required, ask the patient when he or

she last ate.
Procedures:
3. Explain the procedure to the
patient.
4. Prevent the plunger from sticking

by pulling it halfway out and pushing
it all the way in one time.
5. Select the proper tubes to transfer

the blood to after collection. Place in
the proper order for filling.
Procedures:
6. Apply the tourniquet 3-4 inches
above the puncture site.
Do not put the tourniquet on too
tightly or leave it on the patient
longer than 1 minute.
7. Ask the patient to close his or

her hand. The patient must not be
allowed to pump the hand.
8. Place the patient’s arm in

downward position if possible.
Procedures:
9. Select the vein, noting the location and direction of
the vein.
10. Clean the venipuncture with 70% isopropyl alcohol

swab. Cleanse the area in a circular motion,
beginning at the site and working outward.
11. Put on gloves while the alcohol is drying. Do not

touch the venipuncture site.
* If you find it necessary to reevaluate the site by

palpation, the area needs to be re-cleansed before
the venipuncture is performed.
Procedures:
12. Draw the patient’s skin taut with your thumb. The
thumb should be 1 to 2 inches below the puncture site.
13. With the bevel up, align the needle with the vein
and perform venipuncture. While securely grasping the
syringe with one hand, use the other hand slowly pull
the plunger back until the desired amount of blood has
been obtained. Avoid excess probing.
• 15-30 degree angle from the arm surface (arm site)
Note: The larger median cubital* and cephalic veins are the usual choice
for venipuncture, but the basilic vein on the dorsum of the arm or dorsal
hand veins are also acceptable. Foot veins are a last resort because of the
higher probability of complications.
Procedures:
14. Release the tourniquet and ask the patient to
release the fist as soon as the blood flows freely
into the first tube or is established in the syringe.
15. Lightly place gauze square or cotton ball

above the venipuncture site. Gently remove the
needle from the arm.
16. Activate the safety shield over the needle (if

applicable).
17. Apply pressure to the site for 3 to 5 minutes.

The patient may assist if able. This is to avoid
formation of a hematoma.
Procedures:
*18. Aliquot blood into appropriate tubes.
- By puncturing the evacuated tubes
- By manual transfer of the blood into the tubes
*19. Remove the needle from the syringe,

and discard the needle into the sharps
container.
20. Dispose the syringe and transfer device

into sharps container. Do not disconnect
the syringe from the transfer device or
needle before disposal.
Procedures:
21. Recheck the identification bracelet with the labels or requisitions.
22. Label all tubes
23. Check the puncture site. Apply adhesive bandage.

*If the patient is infant, the use of bandage should be eliminated
24. Remove gloves and wash hands.
Last name, First name, MI

Patient ID
Date of extraction – time Extractor Initials
Procedures:
25. Thank the patient and transport the sample(s) to the
laboratory.
Sources:
• McCall, R. and Tankersley, C. (2011). Phlebotomy essentials. 5th ed.
Philadelphia: Wolters Kluwer Health.
• Hoeltke, L. (2013). The complete textbook of phlebotomy. 4th ed.
United States of America: Delmar Cengage Learning.
• Keohane, E., Smith, L. and Walenga, J. (2019). Rodak’s Hematology.
5th ed. Saunders.

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University of Santo Tomas

2. If fasting sample is required, ask the patient when he or

4. Prevent the plunger from sticking

5. Select the proper tubes to transfer

7. Ask the patient to close his or

8. Place the patient’s arm in

10. Clean the venipuncture with 70% isopropyl alcohol

11. Put on gloves while the alcohol is drying. Do not

* If you find it necessary to reevaluate the site by

15. Lightly place gauze square or cotton ball

16. Activate the safety shield over the needle (if

17. Apply pressure to the site for 3 to 5 minutes.

*19. Remove the needle from the syringe,

20. Dispose the syringe and transfer device

22. Label all tubes

23. Check the puncture site. Apply adhesive bandage.

Last name, First name, MI

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