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    Ear Cultures Principle: 3.6.12 Sop: Ear Culture Page 1 of 2

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    SemeeeJunior
    This standard operating procedure outlines the process for collecting and culturing ear swabs to diagnose otitis externa, an infection of the outer ear. Ear swabs are collected using sterile swabs and transported in media, then direct smears and cultures are performed on blood agar, chocolate agar, and other media. Significant growth of bacteria like Staphylococcus aureus or Pseudomonas aeruginosa indicates infection. Reports describe relevant findings from smears and cultures, including identification and susceptibility testing of significant isolates.

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    Ear Cultures Principle: 3.6.12 Sop: Ear Culture Page 1 of 2

    Uploaded by

    SemeeeJunior
    170 views2 pages
    This standard operating procedure outlines the process for collecting and culturing ear swabs to diagnose otitis externa, an infection of the outer ear. Ear swabs are collected using sterile swabs and transported in media, then direct smears and cultures are performed on blood agar, chocolate agar, and other media. Significant growth of bacteria like Staphylococcus aureus or Pseudomonas aeruginosa indicates infection. Reports describe relevant findings from smears and cultures, including identification and susceptibility testing of significant isolates.

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    3.6.12 SOP_Ear Culture

    Original Title

    3.6.12 SOP_Ear Culture

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    This standard operating procedure outlines the process for collecting and culturing ear swabs to diagnose otitis externa, an infection of the outer ear. Ear swabs are collected using sterile swabs and transported in media, then direct smears and cultures are performed on blood agar, chocolate agar, and other media. Significant growth of bacteria like Staphylococcus aureus or Pseudomonas aeruginosa indicates infection. Reports describe relevant findings from smears and cultures, including identification and susceptibility testing of significant isolates.

    Copyright:

    © All Rights Reserved
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    170 views2 pages

    Ear Cultures Principle: 3.6.12 Sop: Ear Culture Page 1 of 2

    Uploaded by

    SemeeeJunior
    This standard operating procedure outlines the process for collecting and culturing ear swabs to diagnose otitis externa, an infection of the outer ear. Ear swabs are collected using sterile swabs and transported in media, then direct smears and cultures are performed on blood agar, chocolate agar, and other media. Significant growth of bacteria like Staphylococcus aureus or Pseudomonas aeruginosa indicates infection. Reports describe relevant findings from smears and cultures, including identification and susceptibility testing of significant isolates.

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    © All Rights Reserved
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    Download as doc, pdf, or txt
    of 2

    STANDARD OPERATING PROCEDURE

    EAR CULTURES

    1. Principle

    Ear swabs are generally collected and sent to the laboratory for the diagnosis of otitis
    externa, or infections of the outer ear. They are not useful in the diagnosis of otitis media,
    or infections of the inner ear. Otitis externa is a bacterial infection of the external
    auditory canal usually caused by P. aeruginosa, S. aureus, S. pneumoniae, Group A
    streptococcus, or fungus / yeast.

    2. Materials

    a. Gram smear: clean glass slide, gram smear reagents


    b. Media: Blood Agar (BAP) and Chocolate Agar (CHOC), Mueller-Hinton agar

    c. Incubator, 35-37°C with 5% CO2 (or a candle jar)


    d. Inoculating loops
    e. Reagents and discs required for organism identification and susceptibility testing
    3. Specimen

    The ear swab is collected using a clean, sterile swab and sent in transport medium. If a
    delay in transport or processing is anticipated, the specimen should be kept refrigerated.

    4. Quality Control (QC)

    Process the specimen as soon after receipt as possible. If there is a delay in processing
    place the specimen in the refrigerator.

    Check that the patient name and identifiers on the specimen match that on the
    accompanying requisition.

    Ensure that all media and supplies used have passed the required QC and are used
    within their expiry date.

    5. Safety Precautions

    Standard safety precautions for handling of patient specimens must be applied when
    processing these specimens:

    6. Procedure

    Processing of specimens:

    Direct smear: Use a clean dry glass slide to prepare the smear, allow to dry, fix, and
    stain.

    Examine the slide under the microscope; quantitate polymorphonuclear leukocytes


    (PMNs), squamous epithelial cells and organisms seen.
    3.6.12 SOP: Ear Culture Page 1 of 2
    STANDARD OPERATING PROCEDURE

    Culture: Note that the culture plates are inoculated prior to the preparation of the smear.

    Rub the swab over a portion of the plates and then streak the inoculums in 4 quadrants
    to obtain isolated colonies. Incubate the plates in 5% CO2.

    7. Interpretation

    Examine the culture plates after 24 and 48 hours incubation.

    Any growth of S. aureus, P. aeruginosa, S. pneumoniae, Group A streptococcus or yeast


    is significant.

    For other organisms, a significant result is determined by the presence of moderate to


    heavy growth of an organism which correlates with the predominant organism on the
    Gram smear. The Gram smear should also show >1+ pus cells.

    Full identification and susceptibility testing is required for all significant organisms
    except yeast.

    8. Reporting

    a) Gram stain: Report with quantitation the presence of pus cells and organisms.

    b) Culture:

    Negative Report: "Commensal flora" or "No growth".

    Positive Report: Quantitate all significant isolates and report with appropriate
    antimicrobial susceptibilities.

    If commensal flora is also present, report with quantitation.

    9. Procedural Notes

    The external ear canal is colonized with normal skin flora such as coagulase negative
    staphylococci, diphtheroids, alpha-hemolytic streptococci and Neisseria sp. The
    isolation of these organisms is generally considered to be contamination.

    10. References

    Clinical Microbiology Procedures Handbook, 3rd Edition, 2010. ASM Press, Washington
    DC.

    3.6.12 SOP: Ear Culture Page 2 of 2

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