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    ExPASy ProtParam Tool

    Uploaded by

    Nestor Stiel
    This document provides a summary of the analysis of a 109 amino acid protein sequence using the ExPASy ProtParam tool. The analysis includes the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficients, estimated half-life, instability index, aliphatic index and grand average of hydropathicity. The instability index classifies the protein as unstable.

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    Download as PDF, TXT or read online from Scribd

    ExPASy ProtParam Tool

    Uploaded by

    Nestor Stiel
    192 views2 pages
    This document provides a summary of the analysis of a 109 amino acid protein sequence using the ExPASy ProtParam tool. The analysis includes the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficients, estimated half-life, instability index, aliphatic index and grand average of hydropathicity. The instability index classifies the protein as unstable.

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    This document provides a summary of the analysis of a 109 amino acid protein sequence using the ExPASy ProtParam tool. The analysis includes the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficients, estimated half-life, instability index, aliphatic index and grand average of hydropathicity. The instability index classifies the protein as unstable.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    192 views2 pages

    ExPASy ProtParam Tool

    Uploaded by

    Nestor Stiel
    This document provides a summary of the analysis of a 109 amino acid protein sequence using the ExPASy ProtParam tool. The analysis includes the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficients, estimated half-life, instability index, aliphatic index and grand average of hydropathicity. The instability index classifies the protein as unstable.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    You are on page 1of 2

    18/06/13

    ExPASy ProtParam tool

    ProtParam
    ProtParam
    User-provided sequence:
    1 0 2 0 3 0 4 0 5 0 6 0 M A L W M R L L P LL A L L A L W G P DP A A A F V N Q H LC G S H L V E A L YL V C G E R G F F YT P K T R R E A E D 7 0 8 0 9 0 1 0 0 L Q V G Q V E L G GG P G A G S L Q P LA L E G S L Q K R GI V E Q C C T S I CS L Y Q L E N Y C

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    References and documentation are available. Please note the modified algorithm for extinction coefficient.

    N u m b e ro fa m i n oa c i d s :1 0 9 M o l e c u l a rw e i g h t :1 1 8 6 6 . 8 T h e o r e t i c a lp I :5 . 2 2 A m i n oa c i dc o m p o s i t i o n : CSV format A l a( A ) 1 0 A r g( R ) 5 A s n( N ) 2 A s p( D ) 2 C y s( C ) 6 G l n( Q ) 7 G l u( E ) 8 G l y( G ) 1 2 H i s( H ) 2 I l e( I ) 2 L e u( L ) 2 0 L y s( K ) 2 M e t( M ) 2 P h e( F ) 3 P r o( P ) 6 S e r( S ) 5 T h r( T ) 3 T r p( W ) 2 T y r( Y ) 4 V a l( V ) 6 P y l( O ) 0 S e c( U ) 0 ( B ) ( Z ) ( X ) 0 0 0 9 . 2 % 4 . 6 % 1 . 8 % 1 . 8 % 5 . 5 % 6 . 4 % 7 . 3 % 1 1 . 0 % 1 . 8 % 1 . 8 % 1 8 . 3 % 1 . 8 % 1 . 8 % 2 . 8 % 5 . 5 % 4 . 6 % 2 . 8 % 1 . 8 % 3 . 7 % 5 . 5 % 0 . 0 % 0 . 0 % 0 . 0 % 0 . 0 % 0 . 0 %

    T o t a ln u m b e ro fn e g a t i v e l yc h a r g e dr e s i d u e s( A s p+G l u ) :1 0 T o t a ln u m b e ro fp o s i t i v e l yc h a r g e dr e s i d u e s( A r g+L y s ) :7 A t o m i cc o m p o s i t i o n : C a r b o n H y d r o g e n C H 5 3 1 8 3 5
    1/2

    web.expasy.org/cgi-bin/protparam/protparam

    18/06/13

    ExPASy ProtParam tool

    N i t r o g e n O x y g e n S u l f u r

    N O S

    1 4 1 1 5 1 8

    F o r m u l a :C H N O S 5 3 1 8 3 5 1 4 1 1 5 1 8 T o t a ln u m b e ro fa t o m s :1 6 6 6 E x t i n c t i o nc o e f f i c i e n t s :
    1c 1 E x t i n c t i o nc o e f f i c i e n t sa r ei nu n i t so f M m ,a t2 8 0n mm e a s u r e di nw a t e r .

    E x t .c o e f f i c i e n t A b s0 . 1 %( = 1g / l )

    1 7 3 3 5 1 . 4 6 1 ,a s s u m i n ga l lp a i r so fC y sr e s i d u e sf o r mc y s t i n e s

    E x t .c o e f f i c i e n t A b s0 . 1 %( = 1g / l )

    1 6 9 6 0 1 . 4 2 9 ,a s s u m i n ga l lC y sr e s i d u e sa r er e d u c e d

    E s t i m a t e dh a l f l i f e : T h eN t e r m i n a lo ft h es e q u e n c ec o n s i d e r e di sM( M e t ) . T h ee s t i m a t e dh a l f l i f ei s :3 0h o u r s( m a m m a l i a nr e t i c u l o c y t e s ,i nv i t r o ) . > 2 0h o u r s( y e a s t ,i nv i v o ) . > 1 0h o u r s( E s c h e r i c h i ac o l i ,i nv i v o ) .

    I n s t a b i l i t yi n d e x : T h ei n s t a b i l i t yi n d e x( I I )i sc o m p u t e dt ob e4 0 . 6 1 T h i sc l a s s i f i e st h ep r o t e i na su n s t a b l e .

    A l i p h a t i ci n d e x :1 0 3 . 8 5 G r a n da v e r a g eo fh y d r o p a t h i c i t y( G R A V Y ) :0 . 2 2 7

    SIB Swiss Institute of Bioinform atics | Disclaim e r

    web.expasy.org/cgi-bin/protparam/protparam

    2/2

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