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    Test For Amino Acids

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    Christian Villanueva
    The document describes several common protein and amino acid tests, including: 1. The Biuret test which detects two or more peptide bonds and results in a purple color from coordination between copper and peptide nitrogen atoms. 2. The Ninhydrin test which detects alpha-amino groups using ninhydrin and results in a purple or blue color. 3. The Xanthoproteic test which detects aromatic amino acids like tryptophan and tyrosine and results in a yellow color.

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    Test For Amino Acids

    Uploaded by

    Christian Villanueva
    2K views2 pages
    The document describes several common protein and amino acid tests, including: 1. The Biuret test which detects two or more peptide bonds and results in a purple color from coordination between copper and peptide nitrogen atoms. 2. The Ninhydrin test which detects alpha-amino groups using ninhydrin and results in a purple or blue color. 3. The Xanthoproteic test which detects aromatic amino acids like tryptophan and tyrosine and results in a yellow color.

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    the different tests for amino acids

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    Test for Amino Acids

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    The document describes several common protein and amino acid tests, including: 1. The Biuret test which detects two or more peptide bonds and results in a purple color from coordination between copper and peptide nitrogen atoms. 2. The Ninhydrin test which detects alpha-amino groups using ninhydrin and results in a purple or blue color. 3. The Xanthoproteic test which detects aromatic amino acids like tryptophan and tyrosine and results in a yellow color.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    2K views2 pages

    Test For Amino Acids

    Uploaded by

    Christian Villanueva
    The document describes several common protein and amino acid tests, including: 1. The Biuret test which detects two or more peptide bonds and results in a purple color from coordination between copper and peptide nitrogen atoms. 2. The Ninhydrin test which detects alpha-amino groups using ninhydrin and results in a purple or blue color. 3. The Xanthoproteic test which detects aromatic amino acids like tryptophan and tyrosine and results in a yellow color.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 2

    Test Biuret Test

    Reagents CuSO4 sol'n, diluted NaOH

    Test for Intact proteins and protein hydrlysates (at least tripeptide, 2 peptide bonds

    Result Pink to violet blue color

    Reaction/Principle Complexation Form. Of coordination complex Cu+2 and 4 N atoms (2 from each 2 polypeptide chain)

    Ninhyrin Test

    1,2,3-indanetrione monohydrate or triketohydintene hydrate, ethanol Conc. HNO3, conc. NaOH

    Alpha- amino group (usually a general test for amino acids)

    Xanthoproteic Test

    For W,F,Y (aromatic except for H)

    Blue to blue-violet Oxidative decarboxylation color & deamination followed by (proline:hydroxypr condensation oline gives a yellow color) Yellow sol'n/ppt. Nitration via SEAr with conc. HNO3, orange with excess NaOH

    Millon's rgt.(Hg2+ & Hg22+nitrates& nitriles/Hg(NO3)2 in conc. HNO3, w/ trace of HNO2/HgSO4,H2SO4 ,NaNO2) Hopkins-Cole Test Glyoxylic acid (Mg powder, oxalic acid, HOAc), conc. H2SO4 Millon's Test

    Phenolic group of Y

    Old rose/flesh to red ppt.

    Complexation(mercuration & nitration or nitrosation/complexation of nitrohydroxyphenyl derivatives with Hg2+)

    Indole group in W

    Pink to violet interface

    Reduction of oxalic acid to glyoxolic acid and acidcatalyzed condensation of two tryptphans w/glyoxolic acid

    Sakaguchi Test

    Alpha-naphthol, NaOBr, NaOH, urea(to stabilize color & destroy excess OBr ions)

    Un-or monosubstituted guanidines in R

    Red to red-orange Complexation (basecolor catalyzed condensation of alpaha-naphthol w/ the guanidine group of R)

    Fohl's Test

    Pb(CH3COO)2 NaOH

    Sulfur-containing C-C)

    (C,

    Brown to black ppt.

    Degradation & substitution rxn to form PbS

    Nitroprusside Test Test for Amide Pauly's Test

    Na2Fe(CN)3 in dil. NH3 NaOH Diazo rgt. (1% sulfosalicylic acid with 5% NaNO2), 10% Na2CO3

    For cys 10,20,30 amides and nitriles ; peptide bonds Detects tyr and his

    Red coloration Red litmus to blue Red coloration

    Complexation rxn Basic hydrolysis Formation of azo dyes

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