Search Results (1,051)

Although a lot of recent research revealed advantages of novel biopolymers’ implementation as active food packaging polymers, there is not an equivalent effort from industry to use such films, probably because of the required cost to change the supply chain and the equipment. This study investigates the use of two natural abundant nanoclays, laponite (Lap) and montmorillonite (Mt), as eugenol slow-release carriers for enhancing the functionality of low-density polyethylene (LDPE) active packaging films. The target is to combine the spirit of the circular economy with the existent technology and the broadly used materials to develop a novel attractive product for active food packaging applications. Utilizing a vacuum-assisted adsorption method, eugenol was successfully intercalated into Lap and Mt nanoclays, forming EG@Lap and EG@Mt nanohybrids. Testing results confirmed effective integration and dispersion of the nanohybrids within the LDPE matrix. The most promising final film seems to be the LDPE with 15% w/w EG@Lap nanohybrid which exhibited a higher release rate (k₂ = 5.29 × 10⁻⁴ s⁻¹) for temperatures ≤70 °C, similar mechanical properties, a significantly improved water barrier (D_wv = 11.7 × 10⁻⁵ cm²·s⁻¹), and a slightly improved oxygen barrier (Pe_O2 = 2.03 × 10⁻⁸ cm²·s⁻¹) compared with neat LDPE. Antimicrobial and sensory tests on fresh minced pork showed two days’ shelf-life extension compared to pure LDPE and one more day compared to LDPE with 15% w/w EG@Mt nanohybrid. Full article

Objectives: To report ophthalmological and microvascular findings in patients with post-acute COVID-19. Methods: In this prospective, monocentric cohort study, we included patients with post-acute COVID-19 who presented with ophthalmological symptoms. All patients underwent indocyanine green angiography (ICGA), OCT, OCT-angiography, adaptive optics, and GlycoCheck assessments. Results: We included 44 patients, predominantly female (81.8%), with a mean age of 47.5 ± 11.5 years. Key ICGA findings revealed hyperreflective dots in 32 eyes (36.4%) and hemangioma-like lesions in 7 eyes (8.0%). Capillary non-perfusion in the superficial capillary plexus (SCP) and deep capillary plexus (DCP) was observed in 42 eyes (47.7%) and 21 eyes (23.9%), respectively. Eyes with hyperreflective dots exhibited a lower perfused boundary region (PBR), while those with superficial punctate keratitis showed a higher PBR (p = 0.02 and p = 0.002, respectively). Eyes with capillary non-perfusion in the SCP displayed lower capillary densities (CD4, CD5, and CD4-6; p = 0.001, 0.03, and 0.03, respectively), and eyes with non-perfusion in the DCP had lower CD4 (p = 0.03). A negative correlation was identified between capillary density and the wall-to-lumen ratio. Conclusions: Patients with post-acute COVID-19 demonstrate both retinal and choroidal vascular anomalies. Ocular pathology was associated with reduced capillary density. These injuries appear to stem more from microvascular disruptions than from persistent glycocalyx abnormalities. Full article

Background: Saryarka sheep belong to fat-tailed coarse-wool sheep breed. This breed is distinguished by increased meat productivity while being competitive in young lamb production. Live weight and body indices are relevant data for assessing sheep body constitution, which directly affects the breeding characteristics and meat productivity of animals. Objectives: This study aimed to find associations with SNPs and nine phenotypic characteristics of the Saryarka fat-tailed coarse-wool sheep breed including live weight and eight body indices (wither height, rump height, bicoastal diameter, body depth, body length, rump width, heart girth, and cannon bone circumference), and find candidate genes related to these characteristics. Methods: A total of 100 animals from the Karaganda region of Kazakhstan were used in this study. Live weight and eight body indices of sheep were measured using tape and electronic scales. The blood samples of the animals were used for DNA extraction. DNA samples were genotyped with the OvineSNP50 Genotyping BeadChip and analyzed using GWAS. Statistically significant SNPs were identified for each characteristic trait referencing the genome of Ovis aries (Oar_v3.1) using BioMart. Results: The GWAS results demonstrated a substantial chromosomal-level correlation between 32 chromosome-wide significant and suggestively significant SNPs in the studied sheep breed. Overall, seven SNPs located in seven different genes were revealed as candidates for live weight and four body indices: s20793.1 SNP in the IGFBP6 gene for live weight, OAR4_54217431.1 SNP in the ST7 gene for bicoastal diameter, s25229.1 in the SCD5 gene, and s01175.1 SNP in the DTNBP1 gene for rump width, OAR2_175574781.1 SNP in the KYNU gene for heart girth, and OAR1_209022621.1 SNP in the FGF12 gene and s15415.1 SNP in the FTO gene for cannon bone circumference. Some of these genes were previously reported to be involved in body constitution and fat deposit in other sheep breeds. Conclusions: The results of the present study open up new opportunities for targeted sheep breeding for meat and fat productivity. Full article

In the absence of data on the reporting of L. monocytogenes resistance to antibiotics, we sought to determine which clonal complexes (CCs)/sequence types (STs) circulate in the food chain in Kosovo and to determine their antibiogram profiles to a panel of 18 antibiotics. From a total of 114 isolates, 21 different typical STs were identified by multilocus sequence typing (MLST). Each isolate derived from the food categories was subjected to tests to verify its susceptibility to the selected antibiotics according to the designed Sensititre GPN3F panel. Among the different STs that were identified, CC9-ST9 was more abundant in meat products (38.75%) while CC29-ST29 was more abundant (24.0%) in dairy products. Moreover, these isolates showed marked resistance against levofloxacin (22.8%), gentamicin and rifampicin (17.5%), quinupristin/dalfopristin (14.9%), erythromycin (11.4%), penicillin (7.89%), tetracycline (1.75%), and streptomycin (0.88%). A total of 27 multiple antibiotic resistance (MAR) phenotypes were observed amongst the isolates, which ranged from 3 to 12. The ARI of the food category including meat and meat products (MMP, 0.22) and fish meat products (FMP, 0.26) were >0.2, the permissible Krumperman threshold. The number of strains with MAR values >0.2 was 34, (29.8%). The identification of typical multidrug-resistant STs among L. monocytogenes isolates in Kosovo constitutes a potential threat to food safety and public health, which requires a continuous and expanded surveillance system to prevent the further spread of antimicrobial resistant (AMR) isolates. Full article

Unlike terrestrial radio, the speed of sound in the ocean is relatively slow, which results in mobile underwater M-ary spread spectrum communication typically exhibiting significant and variable multipath effects along with strong Doppler effects, leading to rapid carrier phase shifts in the received signal that severely impact decoding accuracy. This study aims to address the issue of rapid carrier phase shifts caused by significant time-varying Doppler shifts during mobile underwater M-SS communication. This paper innovatively proposes a method for updating matched filters based on correlation cost factors. By calculating the correlation cost factors for each received symbol, the method guides the direction of Doppler estimation and updates the matched filters. After identifying the optimal match, the received symbols are shifted, correlated, and decoded. Simulation and sea trial results indicate that this method demonstrates higher computational efficiency and improved decoding accuracy compared to traditional Doppler estimation matched filters under low signal-to-noise ratio conditions, and exhibits greater robustness under complex motion conditions. Full article

Background/Objectives: Non-obstructive azoospermia (NOA) is a severe form of male infertility characterized by the absence of sperm in the ejaculate due to impaired spermatogenesis. Testicular sperm extraction (TESE) combined with intracytoplasmic sperm injection is the primary treatment, but success rates are unpredictable, causing significant emotional and financial burdens. Traditional clinical and hormonal predictors have shown inconsistent reliability. This review aims to evaluate current and emerging non-invasive preoperative predictors of successful sperm retrieval in men with NOA, highlighting promising biomarkers and their potential clinical applications. Methods: A comprehensive literature review was conducted, examining studies on clinical and hormonal factors, imaging techniques, molecular biology biomarkers, and genetic testing related to TESE outcomes in NOA patients. The potential role of artificial intelligence and machine learning in enhancing predictive models was also explored. Results: Traditional predictors such as patient age, body mass index, infertility duration, testicular volume, and serum hormone levels (follicle-stimulating hormone, luteinizing hormone, inhibin B) have limited predictive value for TESE success. Emerging non-invasive biomarkers—including anti-Müllerian hormone levels, inhibin B to anti-Müllerian hormone ratio, specific microRNAs, long non-coding RNAs, circular RNAs, and germ-cell-specific proteins like TEX101—show promise in predicting successful sperm retrieval. Advanced imaging techniques like high-frequency ultrasound and functional magnetic resonance imaging offer potential but require further validation. Integrating molecular biomarkers with artificial intelligence and machine learning algorithms may enhance predictive accuracy. Conclusions: Predicting TESE outcomes in men with NOA remains challenging using conventional clinical and hormonal parameters. Emerging non-invasive biomarkers offer significant potential to improve predictive models but require validation through large-scale studies. Incorporating artificial intelligence and machine learning could further refine predictive accuracy, aiding clinical decision-making and improving patient counseling and treatment strategies in NOA. Full article

Aiming at the problem of estimating the speed of M-ary Phase Shift Keying (MPSK) communication radiated sources and their carrying platform targets, this paper proposes a data-assisted and inter-symbol spectrum analysis-based speed estimation method for MPSK communication radiated sources. The method first demodulates a signal-carrying message symbol from the received MPSK signal; then segments the signal according to the symbol synchronization information and the symbol period; and then compensates the phase of the symbol waveform corresponding to the message data according to the demodulated message symbol; finally combines the phase-compensated symbol waveform data into a two-dimensional matrix and finds the Doppler frequency of the data at the same sampling moment of different symbols using the vertical Fourier transform to obtain the moving target speed. The speed measurement accuracy and anti-noise performance of the method are analyzed through simulation experiments, and the simulation results show that the speed measurement accuracy of the method is 98.5%. Full article

Background and Objectives: Recurrent pregnancy loss (RPL) affects numerous couples worldwide and has traditionally been attributed mainly to maternal factors. However, recent evidence highlights significant paternal influences on pregnancy viability and outcomes. This review aims to comprehensively examine male contributions to pregnancy loss, focusing on underlying mechanisms, novel biomarkers, and integrated strategies for improved reproductive success. Materials and Methods: A comprehensive narrative review was conducted by searching databases including PubMed and Embase for the literature published from January 2004 to October 2024. Studies focusing on paternal influences in RPL—encompassing oxidative stress, genetic and epigenetic mechanisms, health conditions, lifestyle factors, environmental exposures, and advancements in sperm proteomics—were included. Inclusion criteria were peer-reviewed articles in English that directly addressed paternal factors in RPL; studies not meeting these criteria were excluded. Results: The review identified that paternal factors such as advanced age, metabolic and cardiovascular health issues, chronic diseases, lifestyle habits (e.g., smoking, alcohol consumption, poor diet), and environmental exposures significantly affect sperm integrity through mechanisms like oxidative stress, DNA fragmentation, and epigenetic alterations. Advanced paternal age and poor health conditions are associated with increased risks of miscarriage and adverse pregnancy outcomes. Novel sperm proteomic biomarkers have been identified, offering potential for enhanced diagnostics and personalized interventions. Integrated approaches involving multidisciplinary assessments, preventive strategies, and genetic counseling are essential for effectively addressing RPL. Conclusions: Integrating paternal factors into clinical evaluations is crucial for effectively addressing recurrent pregnancy loss. Recognizing and modifying paternal risk factors through lifestyle changes, medical interventions, and environmental management can improve pregnancy outcomes. The findings underscore the need for incorporating paternal assessments into standard care and highlight the importance of future research focusing on standardizing diagnostic protocols, expanding studies on paternal contributions, and integrating proteomic biomarkers into clinical practice to facilitate personalized treatment strategies. Full article

The aim of our research was to evaluate and analyze serum 25(OH) vitamin D and parathyroid hormone (PTH) levels to investigate whether vitamin D deficiency serves as a risk factor for an increased incidence of acute respiratory infections (ARI) in children. Serum PTH levels were used as an indicator of vitamin D sufficiency, as normal PTH levels require an optimal concentration of 25(OH) vitamin D. The study included 129 children, divided into five subgroups: children with acute bronchopneumonia (n = 42), acute laryngotracheitis (n = 7), acute bronchiolitis (n = 32), acute bronchitis (n = 18), and a control group (n = 30). No statistically significant differences in 25(OH)D levels were observed between the overall population of children with ARI and the control group (p = 0.073). However, significant differences in 25(OH)D levels were identified between the control group and children with bronchopneumonia, acute bronchitis, and laryngotracheitis (p < 0.01, p < 0.05). Regarding PTH levels, statistical significance was found between the control group and the acute bronchiolitis group, due to the high percentage of children with hypervitaminosis in this subgroup. These results highlight the crucial role of vitamin D in the onset and progression of acute respiratory tract infections in children, emphasizing its impact on their overall respiratory health. Full article

Active galactic nuclei (AGNs) exhibit significant luminosity variations across the entire electromagnetic spectrum, with timescales ranging from hours to years. Studying the optical variability of AGNs provides crucial insights into their physical properties. In this study, we explore the intra-night optical variability (INOV) of AGNs using data from the TESS satellite’s all-sky survey. We derive differential light curves for a sample of 56 AGNs from 30-min cadence TESS full-frame images. Our analysis confirms that BL Lac objects typically exhibit stronger INOV compared to quasars, which generally show weaker variability. The duty cycle for INOV with an amplitude greater than 3% is approximately $4\pm 0.7\%$ for quasars and $22\pm 5\%$ for BL Lac objects. For INOV with an amplitude exceeding 10%, the corresponding duty cycle decreases to about $0.5\pm 0.3\%$ and $5\pm 2\%$, respectively. Furthermore, we identify a potential linear-log relationship between the duty cycle and the INOV amplitude, based on results from this study and previous findings, particularly from the ARIES program. Full article

Recognizing and classifying localized lesions on apple fruit surfaces during automated sorting is critical for improving product quality and increasing the sustainability of fruit production. This study is aimed at developing sustainable methods for fruit sorting by applying hyperspectral analysis and machine learning to improve product quality and reduce losses. The employed hyperspectral technologies and machine learning algorithms enable the rapid and accurate detection of defects on the surface of fruits, enhancing product quality and reducing the number of rejects, thereby contributing to the sustainability of agriculture. This study seeks to advance commercial fruit quality control by comparing hyperspectral image classification algorithms to detect apple lesions caused by pathogens, including sunburn, scab, and rot, on three apple varieties: Honeycrisp, Gala, and Jonagold. The lesions were confirmed independently using expert judgment, real-time PCR, and 3D fluorimetry, providing a high accuracy of ground truth data and allowing conclusions to be drawn on ways to improve the sustainability and safety of the agrocenosis in which the fruits are grown. Hyperspectral imaging combined with mathematical analysis revealed that Venturia inaequalis is the main pathogen responsible for scab, while Botrytis cinerea and Penicillium expansum are the main causes of rot. This comparative study is important because it provides a detailed analysis of the performance of both supervised and unsupervised classification methods for hyperspectral imagery, which is essential for the development of reliable automated grading systems. Support Vector Machines (SVM) proved to be the most accurate, with the highest average adjusted Rand Index (ARI) scores for sunscald (0.789), scab (0.818), and rot (0.854), making it the preferred approach for classifying apple lesions during grading. K-Means performed well for scab (0.786) and rot (0.84) classes, but showed limitations with lower metrics for other lesion types. A design and technological scheme of an optical system for identifying micro- and macro-damage to fruit tissues is proposed, and the dependence of the percentage of apple damage on the rotation frequency of the sorting line rollers is obtained. The optimal values for the rotation frequency of the rollers, at which the damage to apples is less than 5%, are up to 6 Hz. The results of this study confirm the high potential of hyperspectral data for the non-invasive recognition and classification of apple diseases in automated sorting systems with an accuracy comparable to that of human experts. These results provide valuable insights into the optimization of machine learning algorithms for agricultural applications, contributing to the development of more efficient and accurate fruit quality control systems, improved production sustainability, and the long-term storage of fruits. Full article

This scoping review explores the therapeutic strategies available for managing genitourinary syndrome of menopause (GSM), a condition often underdiagnosed and undertreated despite significantly affecting women’s quality of life. GSM results from decreased estrogen levels during menopause, leading to a range of symptoms including vulvovaginal atrophy and urinary tract issues. Material and Methods: we screened the literature for original studies with “menopause”, “hormonal therapy”, “vulvovaginal atrophy”, “urinary incontinence”, “urinary infections”, “genitourinary syndrome”. Results: A total of 451 relevant articles were retrieved. After screening, 19 articles were included in this scoping review. Discussion: First-line treatments typically include lubricants and moisturizers for short-term symptom relief, while unresolved or severe cases may warrant hormonal treatment. Topical hormonal treatments often have fewer side effects than systemic alternatives. Special attention is given to selective estrogen receptor modulators like ospemifene and steroid hormones like dehydroepiandrosterone (DHEA), which have shown beneficial effects on GSM symptoms. Moreover, innovative therapeutic approaches, such as laser treatment, are discussed in the context of their efficacy and accessibility. The safety of GSM hormonal therapy in women with a history or risk of cancer is also addressed, noting the need for more definitive research in this area. While there is a growing demand for tailored therapy, this scoping review emphasizes the importance of effective communication and counseling to allow women to make informed decisions about their treatment. Overall, this review underscores the need for increased awareness and further research into effective treatment options for GSM. Full article

In Para-judo, the technical actions and tactical situations suitable for scoring in a combat have not been studied. The aim of this research was to determine the technical–tactical patterns of scoring actions in Para-judo, focusing on weight categories. An observational methodology was employed to conduct this study. The sample consisted of all male judokas with visual impairment (n = 172) in the senior category who participated in the World Championships held in Odivelas in 2018. A total of 232 combats and 313 scoring actions were analyzed. To obtain the results, we used different analytical techniques with SPSS: descriptive analysis, chi-square test, and T-pattern analysis. The significance level used was p < 0.05. Key findings show that the majority of scoring actions occurred in the first two minutes of combat, highlighting the importance of early initiative. Techniques such as ashi-waza, te-waza, and sutemi-waza demonstrated particular effectiveness across categories, with a focus on movements like sumi-otoshi, ouchi-gari, and ko-soto-gake. Moreover, the most common grip—lapel-sleeve—proved advantageous, aiding judokas in controlling the bout and achieving scores, especially when coupled with effective transition techniques. The study’s weight-specific analysis further revealed distinct patterns, emphasizing the importance of tailored training approaches. For instance, lighter judokas benefited from countering leg attacks with arm or sacrifice techniques to score waza-ari, while heavier judokas favored leg-to-leg counterattacks, often achieving ippon. The results suggest that judokas and coaches could benefit from a more systematic focus on grip stability, strategic positional work, and transition efficiency, particularly from standing to ground. This research contributes valuable insights into optimal techniques and strategies for visually impaired judokas, providing clear guidelines for training and competition. Full article

Sierra Leone has the fourth-highest child mortality rate in Sub-Saharan Africa. This retrospective study aimed to estimate the nationwide prevalence of fever, acute respiratory infection (ARI), and diarrhea in children under 5 years in Sierra Leone, and to identify the risk factors associated with these diseases. We extracted patient information from the 2019 Sierra Leone Demographic and Health Survey data. Data were analyzed using multivariate logistic regression. In total, 8659, 8652, and 8650 children were included in the analyses of fever, ARI, and diarrhea, respectively. The analysis revealed that the nationwide prevalence rates of fever, ARI, and diarrhea were 16.8%, 14.2%, and 7.2%, respectively. Children aged 12–23 months were found to be the most susceptible. Compared to children consuming unimproved water, the highest prevalence rates of fever, ARI, and diarrhea were observed among children residing in households with tube wells or boreholes. The adjusted odds ratio for diarrhea prevalence among children drinking water from household tube wells or boreholes was 1.47 (95% confidence interval: 1.17–1.84, p < 0.001). This study has several limitations, including recall bias due to parental reporting. We suspect that the diseases may be associated with potential water contamination in tube wells or boreholes. We recommend national-level periodic inspections of water quality and community-level education on water supply management. Full article

Objectives: This study aimed to describe the clinical, optical coherence tomography (OCT) and OCT angiography (OCTA) findings and characteristics in patients with perifoveal exudative vascular anomalous complex (PEVAC) and compare the macular vascular density with the age-matched control group. Methods: We conducted a case–control study to compare demographic information, clinical observations, and OCT/OCTA findings in eyes with PEVAC (n = 5 eyes in 5 patients) and a control group of subjects matched for age (n = 9). The Advanced Retina Imaging (ARI) network algorithms were utilized to evaluate OCTA observations. Statistical analysis was performed by the nonparametric Mann–Whitney U test. Results: Patients with PEVAC had a mean (±SD) age at presentation of 70 ± 12.6 years, the mean follow-up period was 7.8 ± 5.2 months, and unilateral disease was observed. Four out of the five patients in our cohort had a history of systemically treated hypertension and dyslipidemia. Three eyes had lesions in the inner temporal retinal zone, while the remaining two eyes had lesions in the inner inferior or central zone. Retina slab analysis using OCTA showed no significant difference in vascular density parameters between the PEVAC and control groups. Conclusions: Although limited by a small sample size, our study suggests that macular vessel density shows no significant difference between PEVAC cases and control eyes. Full article