While Tweety and Aoogah are merely friends in Tweety's High-Flying Adventure (2000), in King Tweety they are blood-related cousins.
The Lanzarote grape fields the characters visit for the juice tasting are a real location in the Canary Islands. While the grapes are used to make grape juice in the film, in real life they are used to make wine.
This is the first Looney Tunes production to have explicit recognition of non-binary individuals. The Canary Islands' first ruler, General Chirp, is referred to using they/them pronouns, and at the start of Tweety's King Day coronation ball, Harold addresses the crowd as "Bird-ladies, gentledogs, nonbirdnaries, and all other lovlies in attendance", a greeting inclusive of genderless and non-binary individuals.
This film was created to commemorate Tweety Bird's 80th birthday.
The characters Melaney Blank and John Foray are named after famous Looney Tunes voice actors Mel Blanc and June Foray.