Quentin Tarantino originally intended to only have Pai Mei's lips speaking Cantonese, while his voice would be in English, imitating a bad dub job. Tarantino was going to provide the voice himself. In the end, Tarantino abandoned this idea, and Pai Mei (Chia-Hui Liu) speaks in his own voice.
In 2018, Uma Thurman posted footage online of a car accident that occurred in 2002 while filming this movie. At the time, Thurman had voiced her reservations about filming a scene while driving a car over a straight dirt road and had requested a stunt performer. However, since the scene was not considered to be a stunt and the stunt coordinator was not on-set that day, Quentin Tarantino persuaded her to shoot the scene herself. While driving the Karmann Ghia, there was an unexpected turn in the road causing Thurman to lose control of the car and crash it into a tree, leaving her with a concussion and damaged knees. She tried to obtain the footage as proof but Harvey Weinstein reportedly refused to release it unless she signed a document that would release the company from any liability. Years later, Tarantino finally gave her the footage and helped her come forward in the wake of multiple sexual assault charges against Weinstein (with Thurman also claiming to be one of his victims). Thurman and Tarantino admitted that the incident had caused a breach in trust which affected their working relationship for several years, but they reconciled afterwards and remain on good terms ever since.
Robert Rodriguez scored this movie for one dollar. Quentin Tarantino said he would repay him by directing a segment of Rodriguez's project Sin City (2005) for one dollar. This was the scene in which Dwight drives Jackie Boy's corpse.
Uma Thurman and Daryl Hannah did not get along with each other, and reportedly instructed hotel and cinema staff to ensure that they were kept separate from each other during the press tour for Volume 1. They were again at odds at the film's screening at the Cannes Film Festival and ordered separate areas to be created at the after-show party so they wouldn't clash. When the two women won "Best Fight" at the 2005 MTV Movie Awards, only Daryl attended. Uma's absence was conspicuous, considering she had gone the previous year to collect the award for her fight with Chiaki Kuriyama in Volume 1.
The brothel segment where the Bride meets with Esteban Vallejo (Michael Parks) was the last scene of the movie to be shot. It was filmed at a Mexican brothel and all of the female extras were sex workers that worked there.
Quentin Tarantino: [long take] The "Massacre at Two Pines" ends in a single long take. The camera follows The Bride down the aisle away from Bill to the altar, then floats away from the altar, down the aisle, past Bill, and out the door to the awaiting members of the DiVAS. Once the squad enters the chapel, the camera cranes up off the ground where pandemonium inside can be heard until finally fading out.
Quentin Tarantino: [Red Apple Cigarettes] The brand of cigarettes smoked in this film in American Spirits, as seen in close-ups by the eagle logo below the filter. However, a pack of Red Apples, Quentin Tarantino's signature brand of fake cigarettes, can clearly be seen on the table when The Bride meets with Esteban (Michael Parks).