During Handel Fane's interview with Sir John, Fane's cigarette reappears in his hand after he has put it out, and then disappears.
When the jury is questioning Sir John, one of the jury members (Mr. Matthews) leans forward to say "That's right", then is shown with the crowd in the next shot and he suddenly is smoking. This happens twice.
When Sir John interviews Diana in jail, they are shown sitting at opposite ends of a table in long-shot. The widths of the planks that make up the tabletop reveal that, in closeups, they are both seated at the same end of the table.
While Diana and Sir John are talking in the prison, her hands go from both gripping the table to only the right hand gripping the table between shots.
When Mr. Markham introduces his wife to Sir John and when the three of them dine together, Mrs. Markham has a corsage attached to her fur coat. Once the three of them head to the boarding house, the corsage is gone, with no explanation of its disappearance.
At the very end of the scene that the half-caste (Fane) meets Sir John in his office, Sir John can be seen to be saying lines that cannot be heard. The scene fades as his mouth is moving.
At around 80 minutes, when Sir John finishes his conversation with Diana Baring and she is being led away by the wardens, he can be heard and seen uttering a promise to her. His back is turned to the camera, his jaw can be seen, though. The scene keeps going and roughly 2 seconds later he's visibly talking again, but nothing can be heard except for the steps of the women as they leave off-screen.
When Sir John and the Markhams discuss their next move in front of the boarding house (and Hitchcock makes his cameo appearance by walking in front of them), an old-style floor microphone is plainly visible near the left edge of the screen.
At around fifty-one minutes, when Sir John, Ted Markham and his wife take their seats at the dining table. The camera dollies back too much and near the left edge of the screen Alfred Hitchcock is visible as he watches the unfolding scene.
(Probably Hitchcock, but precise identity can't be determined).
When Sir John meets Diana in jail, in the first long shot after they sat down: on the left side of the frame the set wall is missing, the studio background is visible.
When director Hitchcock makes his cameo by walking by the house where the murder was committed, the character Doucie Markham momentarily stops listening to her husband and with a slight smile watches Hitchcock pass by - something the character would not have done for an anonymous stranger.