- His character in Zombieland (2009), at one point, expresses relief at not having to update his status on Facebook. Jesse did not know what a Facebook status was until a crew member explained it. The following year, Jesse played Facebook founder, Mark Zuckerberg.
- Despite his iconic portrayal of Facebook co-founder, Mark Zuckerberg, in The Social Network (2010), Jesse himself does not have a Facebook account.
- Received his very first kiss from Jennifer Beals in Roger Dodger (2002).
- He is five months younger than Henry Cavill, making him the first actor to play Lex Luthor who is younger than the actor playing Superman.
- His mother is a professional clown who performed at children's birthday parties in their hometown of East Brunswick, N.J. for 20 years.
- Very good friends with Andrew Garfield.
- Creator of OneUpMe.com - a wordplay website. He also owns Yugoslavio.com.
- Has been playing the drums since he was age 8.
- He is the third Oscar nominated actor to play "Lex Luthor" after Gene Hackman and Kevin Spacey. He is the only one of the three not to have an Academy Award.
- Played brother to real-life sister Hallie Eisenberg in Holy Rollers (2010).
- His family is Ashkenazi Jewish (his ancestors came to the U.S. from Poland, Russia, and Ukraine).
- Studied at The New School in New York City's Greenwich Village. He applied and was accepted to New York University but declined enrollment to complete a film role.
- Has played two real-life Harvard graduate students - Benjamin Strauss in The Hunting Party (2007) and Mark Zuckerberg in The Social Network (2010).
- Wrote a book of short stories titled "Bream Gives Me Hiccups & Other Stories", and cited the work of Simon Rich (a good friend of his) and B.J. Novak (whose best-selling short story collection was "One More Thing: Stories and Other Stories" as creative inspirations.
- Older brother of Hallie Eisenberg.
- Good friends with Justin Bartha.
- Is the second person born in the 1980s to have been nominated for the Academy Award for Best Actor. The first was Ryan Gosling (born in 1980), and the next three are Eddie Redmayne (born in 1982), Andrew Garfield (born in 1983), and Daniel Kaluuya (born in 1989).
- Favorite film is Crimes and Misdemeanors (1989).
- Has a role in Cursed (2005) alongside Michael Rosenbaum who portrayed Lex Luthor in Smallville (2001).
- Good friends with his "Holy Rollers" co-star Justin Bartha, and attended Bartha's marriage to Lia Smith.
- He is first cousin with Jamey Eisenberg, famous Fantasy Sports Writer.
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