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Five executives who worked for Nasty Corporation are turned into animals by Father Time. They have to fix all the environmental disasters they've caused in return for gems so that they may become humans again.

In a series of magical missions, quick-witted YooHoo and his can-do crew travel the globe to help animals in need.

YooHoo and Friends are back to save the Yootopia suffering from pollution in the environment. In their peaceful village, their new friends are flocking to search for shelter after losing their homes on Earth. YooHoo and Friends get along with their new friends in a town filled with joy and friendship. There is a magical spring in the Yootopia, where people sat that it can make you youthful forever with a couple of sips, it also helps our friends keep pure hearts and the peace in the village. One day, Mr. Big Boss learns about this miraculous spring and plans to make big profits selling this enchanted water. He and his never-helpful-assistants, Oops and Coops, watch for an opportunity to pollute YooHoo and Friends beautiful town and drive them out to build a factory in Yootopia to produce and sell "Live Forever" water bottles.