Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 63 No. 1 pp. 63ñ67, 2006
ISSN 0001-6837
Polish Pharmaceutical Society
IN VITRO DISSOLUTION KINETIC STUDY OF THEOPHYLLINE FROM
HYDROPHILIC AND HYDROPHOBIC MATRICES
HAMZAH M. MASWADEH*, MOHAMMAD H. SEMREEN and ABDULATIF A. ABDULHALIM.
School of Pharmacy, Dep. of Pharmaceutical Technology, Al-Isra University,
P.O. Box 961582, Code No. 11196 Amman, Jordan
Abstract: Oral dosage forms containing 300 mg theophylline in matrix type tablets, were prepared by direct
compression method using two kinds of matrices, glycerylbehenate (hydrophobic), and (hydroxypropyl)methyl
cellulose (hydrophilic). The in vitro release kinetics of these formulations were studied at pH 6.8 using the USP
dissolution apparatus with the paddle assemble. The kinetics of the dissolution process were studied by analyzing the dissolution data using four kinetic equations, the zero-order equation, the first-order equation, the
Higuchi square root equation and the Hixson-Crowell cube root law. The analysis of the dissolution kinetic data
for the theophylline preparations in this study shows that it follows the first order kinetics and the release
process involves erosion / diffusion and an alteration in the surface area and diameter of the matrix system, as
well as in the diffusion path length from the matrix drug load during the dissolution process. This relation is
best described by the use of both the first-order equation and the Hixson-Crowell cube root law.
Keywords: Theophylline, glycerylbehenate, (hydroxypropyl)methyl cellulose, dissolution kinetic.
The aim of the present work was to study the
utility of using glycerylbehenate and (hydroxypropyl)methyl cellulose for the formulation of an
controlled-release anhydrous theophylline matrix
tablets and to study the in vitro release characteristics and kinetics of the prepared formulations. The
kinetics of the dissolution process was studied by
the application of four kinetic equations to the dissolution data, namely, the zero-order, the first-order,
the Higuchi square root and Hixson-Crowell cube
root law equations.
A number of methods and techniques have
been used in the manufacturing of oral extendedrelease dosage forms. Probably the simplest and
least expensive way to control the release of an
active agent, is to disperse it in an inert polymeric
matrix (1). In polymeric system, the active agent is
physically blended with the polymer powder and
then fused together by compression, moulding,
which is a common process in the pharmaceutical
industry (2-4). These dosage forms are designed to
deliver the drug at a controlled and predetermined
rate thus maintaining a therapeutically effective concentration of the drug in the systemic circulation for
a long period of time and therefore reducing the frequency of dosing and improving patient compliance
(5, 6).
Hydrophobic material such as glycerylbehenate for an insoluble matrix carrier, and a water soluble hydrophilic material such as (hydroxypropyl)methyl cellulose have been reported as a most commonly used matrix carriers (7, 8).
Anhydrous theophylline, a xanthine bronchodilator is used in the treatment of both chronic and
acute asthmatic attacks. Due to its low therapeutic
index, careful control of its release from dosage forms
has to be ensured. Faulty formulation may result in
the release of large amounts of theophylline i.e. dose
dumping and hence could produce toxic effects (9).
EXPERIMENTAL
Materials and Methods
(Hydroxypropyl)methyl cellulose (Methocel
K15 M) was obtained from Dow Chemical, USA,
glycerylbehenate (Compritol 888 ATO) from Gattefosse, France, anhydrous theophylline from Sigma
Chemical Co. England, microcrystalline cellulose
(Avicel pH 10,2) from FMC Corporation, USA,
magnesium stearate USP, was from Mallinckrodt
Chesterfield, USA and fumed silicone dioxide
(Aerosil 200) from Degussa, USA.
Preparation of direct compressible tablets
Table 1 shows the tablet formulations for direct
compression. All the ingredients were passed
* Corresponding author: e-mail: maswadehhamza@hotmail.com
63
64
HAMZAH M. MASWADEH et al.
through 125 µm sieve and retained on 90 µm sieve.
The powders were mixed together for 10 min in a
high-speed mixer (Erweka Turbula system S27,
Germany). The tablets were prepared by direct compression using single flat-faced punch 13 mm diameter (Erweka-AR 400E, Germany). The hardness of
tablets was controlled between 120-130 N.
Evaluation of the prepared tablets
Friability was determined using Erweka friabilator (TAR). The uniformity of weight and drug content were determined according to USP2002/NF 23
procedures. Mean values of weight variation, content uniformity and friability are shown in Table 2.
Dissolution studies
Dissolution study was carried out according to
the USP paddle method (Hanson Research Co.,
USA) containing 1000 mL of pH 6.8 dissolution
medium (phosphate buffer was used). The temperature of dissolution medium was controlled at 37OC ±
0.5O and stirring speed was maintained at 50 rpm.
Six tablets from each batch were tested for 8 h.
Samples (5 mL) were withdrawn at predetermined
time intervals and immediately replaced with equal
volumes of dissolution medium. Samples were filtered (0.45 Millipore filter) and then their concentrations were determined using UV/Vis spectrophotometer (Varian Australia) at 272 nm.
RESULTS AND DISCUSSION
It could be observed that the tablets prepared fulfill the USP2002/NF 23 requirements for uniformity of
weight, drug content and friability as shown in Table 2.
Figure 1 and Table 3 showed that a significant
variation exists in the in vitro release pattern of theophylline from the tablets containing (hydroxypropyl)methyl cellulose 50 mg, 75 mg and 100 mg
and those containing glycerylbehenate 50 mg, 75 mg
and 100 mg, respectively. More specifically, 60%,
65% and 38% theophylline dissolved from F1, F2
and F3, while 75%, 40% and 33% theophylline dissolved from F4, F5 and F6, respectively, within 3 h.
The results showed (Figure 1) that the release of
drug from F4, F5 and F6 [(hydroxypropyl)methyl
cellulose] was higher in the first half hour than F1,
F2 and F3 (glycerylbehenate). This is attributed to
the fact that (hydroxypropyl)methyl cellulose tablets
show erosions on their surface early in the process,
so the active agent placed in this area is immediately released to the dissolution medium. Glycerylbehenate tablets show less erosions on their surface
than (hydroxypropyl)methyl cellulose tablets and
there is no hydration or swelling at the first half
hour, so the release remained slow.
A dissolution profile comparison was done
using the similarity factor f2 to compare the dissolution profile of theophylline from prepared tablets
Table 1. The composition of 6 tablet formulations containing glycerylbehenate and (hydroxypropyl)methyl cellulose prepared by direct
compression as described in Materials and Methods.
Formula
Glycerylbehenate
(mg)
(Hydroxypropyl)
methyl cellulose
(mg)
Theophylline
(mg)
Avicel
(mg)
Aerosil
(mg)
Magnesium
stearate
(mg)
F1
F2
F3
F4
F5
F6
50
75
100
___
___
___
___
___
___
50
75
100
300
300
300
300
300
300
340
315
290
340
315
290
3
3
3
3
3
3
7
7
7
7
7
7
Table 2. Mean values and coefficient of variation of the pharmacotechnical tablets parameters
Formula
Uniformity of weight
(mg)
(C.V. %)
% Friability
Drug content
(mg)
(C.V. %)
F1
F2
F3
F4
F5
F6
698.67 ± 1.3
698.12 ± 1.6
699.89 ± 1.3
701.13 ± 0.9
701.02 ± 0.7
698.77 ± 1.4
0.378
0.226
0.306
0.405
0.337
0.502
299.5 ± 0.7
298.8 ± 1.3
298.6 ± 1.4
299.4 ± 1.2
300.2 ± 1.2
297.1 ± 1.5
65
In vitro dissolution kinetic study of theophylline...
Table 3. Dissolution rate constants and r values for all formulations obtained from the application of the zero-order, first-order,
Higuchi square root and Hixson-Crowell cube root equations.
Formula No.
Zero order
rate constant (K0)
First order
rate constant (K1)
Higuchi square root
rate constant (K2)
Hixson-Crowell
rate constant (K3)
1
10.255
r = 0.941
0.332
r = 0.994
38.014
r = 0.983
0.356
r = 0.997
2
9.858
r = 0.914
0.280
r = 0.973
37.010
r = 0.968
0.321
r = 0.982
3
5.349
r = 0.955
0.086
r = 0.976
19.667
r = 0.990
0.121
r = 0.989
4
6.836
r = 0.917
0.300
r = 0.994
25.686
r = 0.972
0.290
r = 0.990
5
4.248
r = 0.962
0.074
r = 0.980
15.555
r = 0.993
0.102
r = 0.992
6
3.550
r = 0.948
0.053
r = 0.965
13.110
r = 0.983
0.077
r = 0.979
7*
6.154
r = 0.980
0.091
r = 0.991
22.094
r = 0.992
0.130
r = 0.9967*
8**
3.950
r = 0.990
0.050
r = 0.995
14.073
r = 0.995
0.075
r = 0.998
*Theophylline capsules (THEOLIN SR 300 mg).** Theophylline tablets (UNIPHYLLIN SR 300 mg). r = correlation coefficient. Number
of data points was 8 for all formulations.
Figure 1. A plot of % dissolved versus time for the dissolution data
in accordance with the zero-order equation. F1 (◊), F2 (▲), F3 (●),
F4 (x), F5 ( ), F6 (∆), F7 (o) and F8 (Æ).
Figure 2. A linear plot of log (% remaining) versus time for the
dissolution data accordance with the first-order equation. F1 (◊),
F2 (▲), F3 (●), F4 (x), F5 ( ), F6 (∆), F7 (o) and F8 (Æ).
with those of commercial SR tablets of theophylline
used in this study. Similarity factor was calculated
from the following equation:
The results obtained from the calculation of f2
factor showed that there is a similarity of dissolution
profiles between F6 and Uniphylin SR tablets, as
well as between F3 and Uniphylin SR tablets, with
f2 values 55 and 56, respectively (f2 > 50, dissolution
profiles are defined as similar) (10, 11).
In order to describe the kinetics of the release
process of drug in the 6 formulations as well as in
the 2 standard commercial formulations, various
equations were used such as the zero-order rate
equation, which describes the systems where the
n
f2 = 50 log {[1 +1/n t=1
∑ Wt (Rt ñ Tt)2 ]-0.5 × 100}
where:
Rt = reference assay at time point t.
Tt = test assay at time point t.
n = is the number of pull points
Wt = optional weight factor.
66
HAMZAH M. MASWADEH et al.
Figure 3. A linear plot of % dissolved versus square root of time
for the dissolution data in accordance with the Higuchi square root
equation. F1 (◊), F2 (▲), F3 (●), F4 (x), F5 ( ), F6 (∆), F7 (o) and
F8 (Æ).
Figure 4. A linear plot of the cube root of the initial concentration
minus the cube root of percent remaining versus time for the dissolution data in accordance with the Hixson-Crowell cube root
law. F1 (◊), F2 (▲), F3 (●), F4 (x), F5 ( ), F6 (∆), F7 (o) and F8
(Æ).
release rate is independent of the concentration of
the dissolved species (12). The first-order equation
describes the release from systems where dissolution rate is dependent on the concentration of the
dissolving species (13). The Higuchi square root
equation describes the release from systems where
the solid drug is dispersed in an insoluble matrix and
the rate of drug release is related to the rate of drug
diffusion (14, 15). The Hixson-Crowell cube root
law describes the release from system where there is
a change in surface area and diameter of the particles or tablets (16, 17). The applicability of all of
these equations was tested in this work.
The dissolution data obtained for all formulations at pH 6.8 were plotted in accordance with the
zero-order equation i.e. percent dissolved as a function of time (Figure 1). It is evident from the figure
that the plots are curvilinear suggesting that the
release process is not zero-order in nature.
Figure 5. A linear plot for the relationship between the first-order
dissolution rate constant (K1) and the Hixson-Crowell cube root
dissolution rate constant (K3).
The dissolution data of all formulations at pH
6.8 were plotted in accordance with the first order
equation, i.e. the logarithm of the percent remained
as a function of time (Figure 2). It is evident from
Figure 2 and Table 3 that a linear relationship was
obtained with r value close to unity for all formulations showing that the release is an apparent firstorder process. This indicates that the amount of drug
released is dependent on the matrix drug load.
The dissolution results at pH 6.8 were plotted
in accordance with the Higuchi square root equation, i.e percent dissolved as a function of the square
root of time (Figure 3). A linear relationship is
obtained after an initial lag time has lapsed in all
cases. The linearity of the plots indicates that the
release process is diffusion-controlled.
The dissolution data were also plotted in accordance with the Hixson-Crowell cube root law, i.e.
the cube root of the initial concentration minus the
cube root of percent remained, as a function of time
(Figure 4). Figure 4 indicates that a linear relationship was obtained in all cases.
The relationship between the first-order dissolution rate constant (K1) and the Hixson-Crowell
cube root dissolution rate constant (K3) is best illustrated by K1 vs K3 for all the formulations (Figure 5).
A linear relationship was obtained according to the
following equation:
K1 = - 0.029 + 1.028 K3 (r = 0.991)
This equation suggests that the slope of the K1 - K3
plot is approximately one, r is very close to unity
and the intercept, 0.029 is near zero. This shows that
the change in surface area, diameter of the dissolved
particles or tablets and the change in diffusion path
length during the dissolution process follow the
cube root law.
In vitro dissolution kinetic study of theophylline...
CONCLUSION
From the results obtained in this work it can be
concluded that a significant variation exists in the in
vitro release pattern of theophylline from the tested
formulations. The use of (hydroxypropyl)methyl
cellulose (hydrophilic polymer) and glycerylbehenate (waxy material) with different amounts can
result in preparation of controlled release tablets
with different release rates.
The analysis of the dissolution kinetic data for
the theophylline preparations in this study shows
that it follows the first order kinetics and the release
process involves erosion / diffusion and an alteration
in the surface area and diameter of the matrix system
as well as in the diffusion path length from the
matrix drug load during the dissolution process. It
appears therefore that, in such situations, both the
first-order equation and the Hixson-Crowell cube
root low can best describe the kinetics of the dissolution process of theophylline from all the tested formulations.
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Received: 25.10.2005