A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …

Bacteria and archaea insert spacer sequences acquired from foreign DNAs into CRISPR
loci to generate immunological memory. The Escherichia coli Cas1–Cas2 complex mediates …

The initial stage of CRISPR–Cas immunity involves the integration of foreign DNA spacer
segments into the host genomic CRISPR locus. The nucleases Cas1 and Cas2 are the only …

Bacteria and archaea generate adaptive immunity against phages and plasmids by integrating
foreign DNA of specific 30–40-base-pair lengths into clustered regularly interspaced …

Bacteria and archaea possess a range of defense mechanisms to combat plasmids and viral
infections. Unique among these are the CRISPR-Cas (clustered regularly interspaced short …

Functional genomics efforts face tradeoffs between number of perturbations examined and
complexity of phenotypes measured. We bridge this gap with Perturb-seq, which combines …

Ribosome profiling has revealed pervasive but largely uncharacterized translation outside
of canonical coding sequences (CDSs). In this work, we exploit a systematic CRISPR-based …

Parallel applications running across thousands of processors must protect themselves from
inevitable system failures. Many applications insulate themselves from failures by …

Bacteria and archaea employ adaptive immunity against foreign genetic elements using
CRISPR-Cas systems. To generate immunological memory, the Cas1-Cas2 protein complex …

The PHD finger protein 1 (PHF1) is essential in epigenetic regulation and genome maintenance.
Here we show that the Tudor domain of human PHF1 binds to histone H3 trimethylated …